BRAF mutation and AKAP9 expression in sporadic papillary thyroid carcinomas
We aimed to determine the BRAF mutation and AKAP9 expression in papillary thyroid carcinomas (PTCs). In this study, we analysed 100 sporadic PTC specimens and we detected mutation in 62.2% of the conventional type PTCs (51/82), in 50% of the follicular variant type PTCs (3/6), in 50% of the diffuse...
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| Veröffentlicht in: | Pathology 2006-06, Vol.38 (3), p.201-204 |
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| creator | Lee, Ju-Han Seok Lee, Eung Kim, Young-Sik Hee Won, Nam Chae, Yang-Seok |
| description | We aimed to determine the BRAF mutation and AKAP9 expression in papillary thyroid carcinomas (PTCs).
In this study, we analysed 100 sporadic PTC specimens and we detected mutation in 62.2% of the conventional type PTCs (51/82), in 50% of the follicular variant type PTCs (3/6), in 50% of the diffuse sclerosing variant type PTCs (1/2), and in 30% of the microcarcinomas (3/10). All mutations involved a T→A transversion at the nucleotide 1796. The cases with BRAF mutation were significantly associated with extrathyroidal extension. We also evaluated the expression of AKAP9 protein by immunohistochemistry. The AKAP9 protein was seen as a single perinuclear dot in all the PTCs. Therefore, 58% of the specimens harboured the BRAF mutation and no case had AKAP9-BRAF fusion in the sporadic PTCs.
Our results suggest that the BRAF mutation can be a useful diagnostic and prognostic marker and a target for exploring novel cancer therapies to treat PTCs. AKAP9- BRAF fusion may be a very rare event in sporadic PTCs. |
| doi_str_mv | 10.1080/00313020600696264 |
| format | Article |
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In this study, we analysed 100 sporadic PTC specimens and we detected mutation in 62.2% of the conventional type PTCs (51/82), in 50% of the follicular variant type PTCs (3/6), in 50% of the diffuse sclerosing variant type PTCs (1/2), and in 30% of the microcarcinomas (3/10). All mutations involved a T→A transversion at the nucleotide 1796. The cases with BRAF mutation were significantly associated with extrathyroidal extension. We also evaluated the expression of AKAP9 protein by immunohistochemistry. The AKAP9 protein was seen as a single perinuclear dot in all the PTCs. Therefore, 58% of the specimens harboured the BRAF mutation and no case had AKAP9-BRAF fusion in the sporadic PTCs.
Our results suggest that the BRAF mutation can be a useful diagnostic and prognostic marker and a target for exploring novel cancer therapies to treat PTCs. AKAP9- BRAF fusion may be a very rare event in sporadic PTCs.</description><identifier>ISSN: 0031-3025</identifier><identifier>EISSN: 1465-3931</identifier><identifier>DOI: 10.1080/00313020600696264</identifier><identifier>PMID: 16753739</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>A Kinase Anchor Proteins ; Adaptor Proteins, Signal Transducing - metabolism ; Adult ; Aged ; Aged, 80 and over ; AKAP9 ; Biological and medical sciences ; Biomarkers, Tumor - metabolism ; BRAF mutation ; Carcinoma, Papillary - classification ; Carcinoma, Papillary - genetics ; Carcinoma, Papillary - secondary ; Cytoskeletal Proteins - metabolism ; DNA Mutational Analysis ; DNA, Neoplasm - analysis ; Endocrinopathies ; Female ; Humans ; Immunohistochemistry ; Investigative techniques, diagnostic techniques (general aspects) ; Lymph Nodes - pathology ; Male ; Malignant tumors ; Medical sciences ; Middle Aged ; Neoplasm Staging ; Papillary carcinoma ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Point Mutation ; Polymerase Chain Reaction ; Proto-Oncogene Proteins B-raf - genetics ; Proto-Oncogene Proteins B-raf - metabolism ; Thyroid Neoplasms - classification ; Thyroid Neoplasms - genetics ; Thyroid Neoplasms - pathology ; Thyroid. Thyroid axis (diseases)</subject><ispartof>Pathology, 2006-06, Vol.38 (3), p.201-204</ispartof><rights>2006 Royal College of Pathologists of Australasia</rights><rights>2006 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2006</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-eb63366994b19766e828d23bf89de5d45e9ae15bb12bed3091d99e07fa24b7643</citedby><cites>FETCH-LOGICAL-c499t-eb63366994b19766e828d23bf89de5d45e9ae15bb12bed3091d99e07fa24b7643</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/00313020600696264$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/00313020600696264$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,61197,61378</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17843197$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16753739$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Ju-Han</creatorcontrib><creatorcontrib>Seok Lee, Eung</creatorcontrib><creatorcontrib>Kim, Young-Sik</creatorcontrib><creatorcontrib>Hee Won, Nam</creatorcontrib><creatorcontrib>Chae, Yang-Seok</creatorcontrib><title>BRAF mutation and AKAP9 expression in sporadic papillary thyroid carcinomas</title><title>Pathology</title><addtitle>Pathology</addtitle><description>We aimed to determine the BRAF mutation and AKAP9 expression in papillary thyroid carcinomas (PTCs).
In this study, we analysed 100 sporadic PTC specimens and we detected mutation in 62.2% of the conventional type PTCs (51/82), in 50% of the follicular variant type PTCs (3/6), in 50% of the diffuse sclerosing variant type PTCs (1/2), and in 30% of the microcarcinomas (3/10). All mutations involved a T→A transversion at the nucleotide 1796. The cases with BRAF mutation were significantly associated with extrathyroidal extension. We also evaluated the expression of AKAP9 protein by immunohistochemistry. The AKAP9 protein was seen as a single perinuclear dot in all the PTCs. Therefore, 58% of the specimens harboured the BRAF mutation and no case had AKAP9-BRAF fusion in the sporadic PTCs.
Our results suggest that the BRAF mutation can be a useful diagnostic and prognostic marker and a target for exploring novel cancer therapies to treat PTCs. AKAP9- BRAF fusion may be a very rare event in sporadic PTCs.</description><subject>A Kinase Anchor Proteins</subject><subject>Adaptor Proteins, Signal Transducing - metabolism</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>AKAP9</subject><subject>Biological and medical sciences</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>BRAF mutation</subject><subject>Carcinoma, Papillary - classification</subject><subject>Carcinoma, Papillary - genetics</subject><subject>Carcinoma, Papillary - secondary</subject><subject>Cytoskeletal Proteins - metabolism</subject><subject>DNA Mutational Analysis</subject><subject>DNA, Neoplasm - analysis</subject><subject>Endocrinopathies</subject><subject>Female</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Lymph Nodes - pathology</subject><subject>Male</subject><subject>Malignant tumors</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Neoplasm Staging</subject><subject>Papillary carcinoma</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Point Mutation</subject><subject>Polymerase Chain Reaction</subject><subject>Proto-Oncogene Proteins B-raf - genetics</subject><subject>Proto-Oncogene Proteins B-raf - metabolism</subject><subject>Thyroid Neoplasms - classification</subject><subject>Thyroid Neoplasms - genetics</subject><subject>Thyroid Neoplasms - pathology</subject><subject>Thyroid. Thyroid axis (diseases)</subject><issn>0031-3025</issn><issn>1465-3931</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFq3DAURUVpaCZpP6Cb4E27cyNZsizR1WTIpGUCCSVZC1l6ZhRsyZHs0vx9NIwhi0JWD_TOfbochL4S_INggS8xpoTiCnOMueQVZx_QijBel1RS8hGtDvsyA_UpOkvpCWPMhBCf0CnhTU0bKldod_VnvS2GedKTC77Q3hbr3fpeFvBvjJDS4dH5Io0hautMMerR9b2OL8W0f4nB2cLoaJwPg06f0Umn-wRflnmOHrfXD5tf5e3dze_N-rY0TMqphJZTyrmUrCWy4RxEJWxF205IC7VlNUgNpG5bUrVgKZbESgm46XTF2oYzeo6-H--OMTzPkCY1uGQg1_IQ5qS4wIwSSTNIjqCJIaUInRqjG3J5RbA6GFT_GcyZi-X43A5g3xKLsgx8WwCdjO67qL1x6Y1rxOHzJnM_j5zzXYiD3oPup322BeopzNFnQ-_WWNKQRf51EFUyDrwB6yKYSdng3km_AnRAnA0</recordid><startdate>20060601</startdate><enddate>20060601</enddate><creator>Lee, Ju-Han</creator><creator>Seok Lee, Eung</creator><creator>Kim, Young-Sik</creator><creator>Hee Won, Nam</creator><creator>Chae, Yang-Seok</creator><general>Elsevier B.V</general><general>Informa UK Ltd</general><general>Taylor and Francis</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060601</creationdate><title>BRAF mutation and AKAP9 expression in sporadic papillary thyroid carcinomas</title><author>Lee, Ju-Han ; Seok Lee, Eung ; Kim, Young-Sik ; Hee Won, Nam ; Chae, Yang-Seok</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-eb63366994b19766e828d23bf89de5d45e9ae15bb12bed3091d99e07fa24b7643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>A Kinase Anchor Proteins</topic><topic>Adaptor Proteins, Signal Transducing - metabolism</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>AKAP9</topic><topic>Biological and medical sciences</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>BRAF mutation</topic><topic>Carcinoma, Papillary - classification</topic><topic>Carcinoma, Papillary - genetics</topic><topic>Carcinoma, Papillary - secondary</topic><topic>Cytoskeletal Proteins - metabolism</topic><topic>DNA Mutational Analysis</topic><topic>DNA, Neoplasm - analysis</topic><topic>Endocrinopathies</topic><topic>Female</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Lymph Nodes - pathology</topic><topic>Male</topic><topic>Malignant tumors</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Neoplasm Staging</topic><topic>Papillary carcinoma</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Point Mutation</topic><topic>Polymerase Chain Reaction</topic><topic>Proto-Oncogene Proteins B-raf - genetics</topic><topic>Proto-Oncogene Proteins B-raf - metabolism</topic><topic>Thyroid Neoplasms - classification</topic><topic>Thyroid Neoplasms - genetics</topic><topic>Thyroid Neoplasms - pathology</topic><topic>Thyroid. Thyroid axis (diseases)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Ju-Han</creatorcontrib><creatorcontrib>Seok Lee, Eung</creatorcontrib><creatorcontrib>Kim, Young-Sik</creatorcontrib><creatorcontrib>Hee Won, Nam</creatorcontrib><creatorcontrib>Chae, Yang-Seok</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Ju-Han</au><au>Seok Lee, Eung</au><au>Kim, Young-Sik</au><au>Hee Won, Nam</au><au>Chae, Yang-Seok</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>BRAF mutation and AKAP9 expression in sporadic papillary thyroid carcinomas</atitle><jtitle>Pathology</jtitle><addtitle>Pathology</addtitle><date>2006-06-01</date><risdate>2006</risdate><volume>38</volume><issue>3</issue><spage>201</spage><epage>204</epage><pages>201-204</pages><issn>0031-3025</issn><eissn>1465-3931</eissn><abstract>We aimed to determine the BRAF mutation and AKAP9 expression in papillary thyroid carcinomas (PTCs).
In this study, we analysed 100 sporadic PTC specimens and we detected mutation in 62.2% of the conventional type PTCs (51/82), in 50% of the follicular variant type PTCs (3/6), in 50% of the diffuse sclerosing variant type PTCs (1/2), and in 30% of the microcarcinomas (3/10). All mutations involved a T→A transversion at the nucleotide 1796. The cases with BRAF mutation were significantly associated with extrathyroidal extension. We also evaluated the expression of AKAP9 protein by immunohistochemistry. The AKAP9 protein was seen as a single perinuclear dot in all the PTCs. Therefore, 58% of the specimens harboured the BRAF mutation and no case had AKAP9-BRAF fusion in the sporadic PTCs.
Our results suggest that the BRAF mutation can be a useful diagnostic and prognostic marker and a target for exploring novel cancer therapies to treat PTCs. AKAP9- BRAF fusion may be a very rare event in sporadic PTCs.</abstract><cop>London</cop><pub>Elsevier B.V</pub><pmid>16753739</pmid><doi>10.1080/00313020600696264</doi><tpages>4</tpages></addata></record> |
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| subjects | A Kinase Anchor Proteins Adaptor Proteins, Signal Transducing - metabolism Adult Aged Aged, 80 and over AKAP9 Biological and medical sciences Biomarkers, Tumor - metabolism BRAF mutation Carcinoma, Papillary - classification Carcinoma, Papillary - genetics Carcinoma, Papillary - secondary Cytoskeletal Proteins - metabolism DNA Mutational Analysis DNA, Neoplasm - analysis Endocrinopathies Female Humans Immunohistochemistry Investigative techniques, diagnostic techniques (general aspects) Lymph Nodes - pathology Male Malignant tumors Medical sciences Middle Aged Neoplasm Staging Papillary carcinoma Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Point Mutation Polymerase Chain Reaction Proto-Oncogene Proteins B-raf - genetics Proto-Oncogene Proteins B-raf - metabolism Thyroid Neoplasms - classification Thyroid Neoplasms - genetics Thyroid Neoplasms - pathology Thyroid. Thyroid axis (diseases) |
| title | BRAF mutation and AKAP9 expression in sporadic papillary thyroid carcinomas |
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