Effect of ionic environments on the adsorption and diffusion characteristics of serine alkaline protease enzyme in polyethersulfone ultrafiltration membranes
Static adsorption of serine alkaline protease (SAP) enzyme on hydrophobic polyether sulfone (PES) ultrafiltration membranes in different ionic environments was investigated. The amount of SAP adsorbed on membranes was the lowest at its isoelectric point (IEP) where the maximum adsorption was obtaine...
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Veröffentlicht in: | Journal of colloid and interface science 2006-07, Vol.299 (2), p.806-814 |
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description | Static adsorption of serine alkaline protease (SAP) enzyme on hydrophobic polyether sulfone (PES) ultrafiltration membranes in different ionic environments was investigated. The amount of SAP adsorbed on membranes was the lowest at its isoelectric point (IEP) where the maximum adsorption was obtained below the IEP of the enzyme. The extent of SAP adsorption in the phosphate buffer solutions including different salts followed the order: (NH
4)
2HPO
4 > KH
2PO
4 > Na
2HPO
4−NaH
2PO
4 (buffer) > CaCl
2 > ((NH
4)
2HPO
4 + H
2PO
4 + CaCl
2), which was consistent with the Hofmeister series. The zeta potentials of membranes contacted with the ionic species were calculated by streaming potential measurements and found that the increase in ionic strength decreased the electrical double layer thickness leading to a decrease in adsorption. A model based on mass balance was developed to calculate the diffusion coefficient of SAP in PES membranes. Employing experimental data evaluated in a diffusion cell along with the data of adsorption isotherms, diffusion coefficients of SAP in PES membranes in the presence of different ionic species were calculated. To detect the structural changes occurred, membrane surfaces were analysed by Fourier transform infrared-attenuated total reflectance (FTIR-ATR) measurements.
Membrane fouling consists of adsorption and aggregation. Determinations of the parameters that affect the membrane fouling and developing methods for fouling analysis are important for membrane separation systems. |
doi_str_mv | 10.1016/j.jcis.2006.03.020 |
format | Article |
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4)
2HPO
4 > KH
2PO
4 > Na
2HPO
4−NaH
2PO
4 (buffer) > CaCl
2 > ((NH
4)
2HPO
4 + H
2PO
4 + CaCl
2), which was consistent with the Hofmeister series. The zeta potentials of membranes contacted with the ionic species were calculated by streaming potential measurements and found that the increase in ionic strength decreased the electrical double layer thickness leading to a decrease in adsorption. A model based on mass balance was developed to calculate the diffusion coefficient of SAP in PES membranes. Employing experimental data evaluated in a diffusion cell along with the data of adsorption isotherms, diffusion coefficients of SAP in PES membranes in the presence of different ionic species were calculated. To detect the structural changes occurred, membrane surfaces were analysed by Fourier transform infrared-attenuated total reflectance (FTIR-ATR) measurements.
Membrane fouling consists of adsorption and aggregation. Determinations of the parameters that affect the membrane fouling and developing methods for fouling analysis are important for membrane separation systems.</description><identifier>ISSN: 0021-9797</identifier><identifier>EISSN: 1095-7103</identifier><identifier>DOI: 10.1016/j.jcis.2006.03.020</identifier><identifier>PMID: 16616180</identifier><identifier>CODEN: JCISA5</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Adsorption ; Buffers ; Chemistry ; Colloidal state and disperse state ; Diffusion ; Diffusion coefficient ; Equipment Design ; Exact sciences and technology ; General and physical chemistry ; Hofmeister series ; Hydrogen-Ion Concentration ; Kinetics ; Membranes ; Membranes, Artificial ; Osmolar Concentration ; Phosphates ; Polymers ; Protease ; Serine Endopeptidases - chemistry ; Serine Endopeptidases - isolation & purification ; Sulfones ; Surface physical chemistry ; Ultrafiltration</subject><ispartof>Journal of colloid and interface science, 2006-07, Vol.299 (2), p.806-814</ispartof><rights>2006 Elsevier Inc.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-8641af9eb748f10ba20ccc66d941e7816b76e1bbcee03c1cb6879750c61348943</citedby><cites>FETCH-LOGICAL-c384t-8641af9eb748f10ba20ccc66d941e7816b76e1bbcee03c1cb6879750c61348943</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jcis.2006.03.020$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17863616$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16616180$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SALGIN, Sema</creatorcontrib><creatorcontrib>TAKAC, Serpil</creatorcontrib><creatorcontrib>ÖZDAMAR, Tuncer H</creatorcontrib><title>Effect of ionic environments on the adsorption and diffusion characteristics of serine alkaline protease enzyme in polyethersulfone ultrafiltration membranes</title><title>Journal of colloid and interface science</title><addtitle>J Colloid Interface Sci</addtitle><description>Static adsorption of serine alkaline protease (SAP) enzyme on hydrophobic polyether sulfone (PES) ultrafiltration membranes in different ionic environments was investigated. The amount of SAP adsorbed on membranes was the lowest at its isoelectric point (IEP) where the maximum adsorption was obtained below the IEP of the enzyme. The extent of SAP adsorption in the phosphate buffer solutions including different salts followed the order: (NH
4)
2HPO
4 > KH
2PO
4 > Na
2HPO
4−NaH
2PO
4 (buffer) > CaCl
2 > ((NH
4)
2HPO
4 + H
2PO
4 + CaCl
2), which was consistent with the Hofmeister series. The zeta potentials of membranes contacted with the ionic species were calculated by streaming potential measurements and found that the increase in ionic strength decreased the electrical double layer thickness leading to a decrease in adsorption. A model based on mass balance was developed to calculate the diffusion coefficient of SAP in PES membranes. Employing experimental data evaluated in a diffusion cell along with the data of adsorption isotherms, diffusion coefficients of SAP in PES membranes in the presence of different ionic species were calculated. To detect the structural changes occurred, membrane surfaces were analysed by Fourier transform infrared-attenuated total reflectance (FTIR-ATR) measurements.
Membrane fouling consists of adsorption and aggregation. Determinations of the parameters that affect the membrane fouling and developing methods for fouling analysis are important for membrane separation systems.</description><subject>Adsorption</subject><subject>Buffers</subject><subject>Chemistry</subject><subject>Colloidal state and disperse state</subject><subject>Diffusion</subject><subject>Diffusion coefficient</subject><subject>Equipment Design</subject><subject>Exact sciences and technology</subject><subject>General and physical chemistry</subject><subject>Hofmeister series</subject><subject>Hydrogen-Ion Concentration</subject><subject>Kinetics</subject><subject>Membranes</subject><subject>Membranes, Artificial</subject><subject>Osmolar Concentration</subject><subject>Phosphates</subject><subject>Polymers</subject><subject>Protease</subject><subject>Serine Endopeptidases - chemistry</subject><subject>Serine Endopeptidases - isolation & purification</subject><subject>Sulfones</subject><subject>Surface physical chemistry</subject><subject>Ultrafiltration</subject><issn>0021-9797</issn><issn>1095-7103</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU2O1DAQhS0EYpqBC7BA3sAuoZykHUdig0bDjzQSG1hbjlPWuEnsxuWM1NyFu-LQLdixsV3SV6-e6zH2UkAtQMi3h_pgPdUNgKyhraGBR2wnYNhXvYD2MdsBNKIa-qG_Ys-IDgBC7PfDU3YlpBRSKNixX7fOoc08Ou5j8JZjePAphgVDJh4Dz_fIzUQxHXMBuAkTn7xzK22VvTfJ2IzJU_aWNhUqRSgt83czb49jihkNYRH-eVqQ-8CPcT5h0U20zi4WZp1zMs5v558hCy5jMgHpOXvizEz44nJfs28fbr_efKruvnz8fPP-rrKt6nKlZCeMG3DsO-UEjKYBa62U09AJ7JWQYy9RjKNFhNYKO0pVlrIHK0XbqaFrr9mbs25x-2NFynrxZHGei4m4kpYKumbfqAI2Z9CmSJTQ6WPyi0knLUBvoeiD3kLRWygaWl1CKU2vLurruOD0r-WSQgFeXwBD1syu_H3T-Mv1SrYFLdy7M4dlFw8ekybrMVicfCoh6in6__n4DaYKr6U</recordid><startdate>20060715</startdate><enddate>20060715</enddate><creator>SALGIN, Sema</creator><creator>TAKAC, Serpil</creator><creator>ÖZDAMAR, Tuncer H</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060715</creationdate><title>Effect of ionic environments on the adsorption and diffusion characteristics of serine alkaline protease enzyme in polyethersulfone ultrafiltration membranes</title><author>SALGIN, Sema ; TAKAC, Serpil ; ÖZDAMAR, Tuncer H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-8641af9eb748f10ba20ccc66d941e7816b76e1bbcee03c1cb6879750c61348943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adsorption</topic><topic>Buffers</topic><topic>Chemistry</topic><topic>Colloidal state and disperse state</topic><topic>Diffusion</topic><topic>Diffusion coefficient</topic><topic>Equipment Design</topic><topic>Exact sciences and technology</topic><topic>General and physical chemistry</topic><topic>Hofmeister series</topic><topic>Hydrogen-Ion Concentration</topic><topic>Kinetics</topic><topic>Membranes</topic><topic>Membranes, Artificial</topic><topic>Osmolar Concentration</topic><topic>Phosphates</topic><topic>Polymers</topic><topic>Protease</topic><topic>Serine Endopeptidases - chemistry</topic><topic>Serine Endopeptidases - isolation & purification</topic><topic>Sulfones</topic><topic>Surface physical chemistry</topic><topic>Ultrafiltration</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SALGIN, Sema</creatorcontrib><creatorcontrib>TAKAC, Serpil</creatorcontrib><creatorcontrib>ÖZDAMAR, Tuncer H</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of colloid and interface science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SALGIN, Sema</au><au>TAKAC, Serpil</au><au>ÖZDAMAR, Tuncer H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of ionic environments on the adsorption and diffusion characteristics of serine alkaline protease enzyme in polyethersulfone ultrafiltration membranes</atitle><jtitle>Journal of colloid and interface science</jtitle><addtitle>J Colloid Interface Sci</addtitle><date>2006-07-15</date><risdate>2006</risdate><volume>299</volume><issue>2</issue><spage>806</spage><epage>814</epage><pages>806-814</pages><issn>0021-9797</issn><eissn>1095-7103</eissn><coden>JCISA5</coden><abstract>Static adsorption of serine alkaline protease (SAP) enzyme on hydrophobic polyether sulfone (PES) ultrafiltration membranes in different ionic environments was investigated. The amount of SAP adsorbed on membranes was the lowest at its isoelectric point (IEP) where the maximum adsorption was obtained below the IEP of the enzyme. The extent of SAP adsorption in the phosphate buffer solutions including different salts followed the order: (NH
4)
2HPO
4 > KH
2PO
4 > Na
2HPO
4−NaH
2PO
4 (buffer) > CaCl
2 > ((NH
4)
2HPO
4 + H
2PO
4 + CaCl
2), which was consistent with the Hofmeister series. The zeta potentials of membranes contacted with the ionic species were calculated by streaming potential measurements and found that the increase in ionic strength decreased the electrical double layer thickness leading to a decrease in adsorption. A model based on mass balance was developed to calculate the diffusion coefficient of SAP in PES membranes. Employing experimental data evaluated in a diffusion cell along with the data of adsorption isotherms, diffusion coefficients of SAP in PES membranes in the presence of different ionic species were calculated. To detect the structural changes occurred, membrane surfaces were analysed by Fourier transform infrared-attenuated total reflectance (FTIR-ATR) measurements.
Membrane fouling consists of adsorption and aggregation. Determinations of the parameters that affect the membrane fouling and developing methods for fouling analysis are important for membrane separation systems.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>16616180</pmid><doi>10.1016/j.jcis.2006.03.020</doi><tpages>9</tpages></addata></record> |
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subjects | Adsorption Buffers Chemistry Colloidal state and disperse state Diffusion Diffusion coefficient Equipment Design Exact sciences and technology General and physical chemistry Hofmeister series Hydrogen-Ion Concentration Kinetics Membranes Membranes, Artificial Osmolar Concentration Phosphates Polymers Protease Serine Endopeptidases - chemistry Serine Endopeptidases - isolation & purification Sulfones Surface physical chemistry Ultrafiltration |
title | Effect of ionic environments on the adsorption and diffusion characteristics of serine alkaline protease enzyme in polyethersulfone ultrafiltration membranes |
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