A high-throughput method for quantifying gene expression data from early Drosophila embryos

We describe an automated high-throughput method to measure protein levels in single nuclei in blastoderm embryos of Drosophila melanogaster by means of immunofluorescence. The method consists of a chain of specific algorithms assembled into an image processing pipeline. This pipeline transforms a co...

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Veröffentlicht in:	Development genes and evolution 2005-07, Vol.215 (7), p.374-381
Hauptverfasser:	Janssens, Hilde, Kosman, Dave, Vanario-Alonso, Carlos E, Jaeger, Johannes, Samsonova, Maria, Reinitz, John
Format:	Artikel
Sprache:	eng
Schlagworte:	Algorithms Animals Antigens, CD20 - metabolism Blastoderm Drosophila - embryology Drosophila - genetics Drosophila Proteins - genetics Drosophila Proteins - metabolism Embryo, Nonmammalian - metabolism Embryos Fluorescent Antibody Technique, Direct Gene expression Gene Expression Regulation, Developmental Genes, Insect Hemoglobins - metabolism Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Image processing Image Processing, Computer-Assisted Immunofluorescence Insect Proteins - genetics Insect Proteins - metabolism Microscopy, Confocal Proteins Time Factors Transcription Factors - genetics Transcription Factors - metabolism
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