Inhibition of in vivo angiogenesis by N-β-alanyl-5- S-glutathionyl-3,4-dihydroxyphenylalanine
N-β-alanyl-5- S-glutathionyl-3,4-dihydroxyphenylalanine (5-S-GAD), an antibacterial substance isolated from the flesh fly, inhibits human tumor growth in the nude mice model; however, the mechanism of its action is unclear. The in vivo antitumor effect includes the inhibition of tumor cell prolifera...
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| Veröffentlicht in: | European journal of pharmacology 2006-06, Vol.539 (3), p.151-157 |
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| container_title | European journal of pharmacology |
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| creator | Nishikawa, Takeshi Akiyama, Nobuko Kunimasa, Kazuhiro Oikawa, Tsutomu Ishizuka, Masaaki Tsujimoto, Masafumi Natori, Shunji |
| description | N-β-alanyl-5-
S-glutathionyl-3,4-dihydroxyphenylalanine (5-S-GAD), an antibacterial substance isolated from the flesh fly, inhibits human tumor growth in the nude mice model; however, the mechanism of its action is unclear. The in vivo antitumor effect includes the inhibition of tumor cell proliferation and suppression of angiogenesis. Angiogenesis is essential for tumor growth in vivo. In this study, we examined whether 5-S-GAD inhibits tumor cell-induced angiogenesis by performing the mouse dorsal air sac assay. We found that intraperitoneal administration of 5-S-GAD inhibited the angiogenesis induced by S180 mouse sarcoma cells. Furthermore, 5-S-GAD also inhibited vascular endothelial growth factor-induced angiogenesis in the Matrigel plug assay and embryonic angiogenesis in the chick embryo chorioallantoic membrane assay. However, 5-S-GAD did not show any effect on the proliferation, migration, and tube formation of vascular endothelial cells. These results provide the first evidence that a bioactive substance derived from the flesh fly has antiangiogenic activity in vivo, although the mechanisms involved could not be explained. |
| doi_str_mv | 10.1016/j.ejphar.2006.03.084 |
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S-glutathionyl-3,4-dihydroxyphenylalanine (5-S-GAD), an antibacterial substance isolated from the flesh fly, inhibits human tumor growth in the nude mice model; however, the mechanism of its action is unclear. The in vivo antitumor effect includes the inhibition of tumor cell proliferation and suppression of angiogenesis. Angiogenesis is essential for tumor growth in vivo. In this study, we examined whether 5-S-GAD inhibits tumor cell-induced angiogenesis by performing the mouse dorsal air sac assay. We found that intraperitoneal administration of 5-S-GAD inhibited the angiogenesis induced by S180 mouse sarcoma cells. Furthermore, 5-S-GAD also inhibited vascular endothelial growth factor-induced angiogenesis in the Matrigel plug assay and embryonic angiogenesis in the chick embryo chorioallantoic membrane assay. However, 5-S-GAD did not show any effect on the proliferation, migration, and tube formation of vascular endothelial cells. These results provide the first evidence that a bioactive substance derived from the flesh fly has antiangiogenic activity in vivo, although the mechanisms involved could not be explained.</description><identifier>ISSN: 0014-2999</identifier><identifier>EISSN: 1879-0712</identifier><identifier>DOI: 10.1016/j.ejphar.2006.03.084</identifier><identifier>PMID: 16725138</identifier><identifier>CODEN: EJPHAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>5-S-GAD ; Angiogenesis ; Angiogenesis Inhibitors - pharmacology ; Angiogenesis Inhibitors - therapeutic use ; Animals ; Antiangiogenic agent ; Biological and medical sciences ; Cell Line, Tumor ; Chick Embryo ; Dihydroxyphenylalanine - analogs & derivatives ; Dihydroxyphenylalanine - pharmacology ; Dihydroxyphenylalanine - therapeutic use ; Dose-Response Relationship, Drug ; Female ; Glutathione - analogs & derivatives ; Glutathione - pharmacology ; Glutathione - therapeutic use ; Humans ; Medical sciences ; Mice ; Mice, Inbred C57BL ; Mice, Inbred ICR ; Neovascularization, Pathologic - drug therapy ; Neovascularization, Pathologic - pathology ; Neovascularization, Physiologic - drug effects ; Neovascularization, Physiologic - physiology ; Pharmacology. Drug treatments ; Tumor therapy ; Xenograft Model Antitumor Assays - methods</subject><ispartof>European journal of pharmacology, 2006-06, Vol.539 (3), p.151-157</ispartof><rights>2006 Elsevier B.V.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-ff551eccea514e56423eae1ef368a973f179c5082c8fdd937ae305d9fde862843</citedby><cites>FETCH-LOGICAL-c390t-ff551eccea514e56423eae1ef368a973f179c5082c8fdd937ae305d9fde862843</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ejphar.2006.03.084$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17824441$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16725138$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nishikawa, Takeshi</creatorcontrib><creatorcontrib>Akiyama, Nobuko</creatorcontrib><creatorcontrib>Kunimasa, Kazuhiro</creatorcontrib><creatorcontrib>Oikawa, Tsutomu</creatorcontrib><creatorcontrib>Ishizuka, Masaaki</creatorcontrib><creatorcontrib>Tsujimoto, Masafumi</creatorcontrib><creatorcontrib>Natori, Shunji</creatorcontrib><title>Inhibition of in vivo angiogenesis by N-β-alanyl-5- S-glutathionyl-3,4-dihydroxyphenylalanine</title><title>European journal of pharmacology</title><addtitle>Eur J Pharmacol</addtitle><description>N-β-alanyl-5-
S-glutathionyl-3,4-dihydroxyphenylalanine (5-S-GAD), an antibacterial substance isolated from the flesh fly, inhibits human tumor growth in the nude mice model; however, the mechanism of its action is unclear. The in vivo antitumor effect includes the inhibition of tumor cell proliferation and suppression of angiogenesis. Angiogenesis is essential for tumor growth in vivo. In this study, we examined whether 5-S-GAD inhibits tumor cell-induced angiogenesis by performing the mouse dorsal air sac assay. We found that intraperitoneal administration of 5-S-GAD inhibited the angiogenesis induced by S180 mouse sarcoma cells. Furthermore, 5-S-GAD also inhibited vascular endothelial growth factor-induced angiogenesis in the Matrigel plug assay and embryonic angiogenesis in the chick embryo chorioallantoic membrane assay. However, 5-S-GAD did not show any effect on the proliferation, migration, and tube formation of vascular endothelial cells. These results provide the first evidence that a bioactive substance derived from the flesh fly has antiangiogenic activity in vivo, although the mechanisms involved could not be explained.</description><subject>5-S-GAD</subject><subject>Angiogenesis</subject><subject>Angiogenesis Inhibitors - pharmacology</subject><subject>Angiogenesis Inhibitors - therapeutic use</subject><subject>Animals</subject><subject>Antiangiogenic agent</subject><subject>Biological and medical sciences</subject><subject>Cell Line, Tumor</subject><subject>Chick Embryo</subject><subject>Dihydroxyphenylalanine - analogs & derivatives</subject><subject>Dihydroxyphenylalanine - pharmacology</subject><subject>Dihydroxyphenylalanine - therapeutic use</subject><subject>Dose-Response Relationship, Drug</subject><subject>Female</subject><subject>Glutathione - analogs & derivatives</subject><subject>Glutathione - pharmacology</subject><subject>Glutathione - therapeutic use</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Inbred ICR</subject><subject>Neovascularization, Pathologic - drug therapy</subject><subject>Neovascularization, Pathologic - pathology</subject><subject>Neovascularization, Physiologic - drug effects</subject><subject>Neovascularization, Physiologic - physiology</subject><subject>Pharmacology. Drug treatments</subject><subject>Tumor therapy</subject><subject>Xenograft Model Antitumor Assays - methods</subject><issn>0014-2999</issn><issn>1879-0712</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM2O0zAUhS0EYsrAGyCUDaxwuP5L7A0SGvEz0ggWwBbLda4bV6lT7LQir8WD8EyktNLsWF3p6DtHVx8hzxnUDFjzZlvjdt-7XHOApgZRg5YPyIrp1lBoGX9IVgBMUm6MuSJPStkCgDJcPSZXrGm5YkKvyI_b1Md1nOKYqjFUMVXHeBwrlzZx3GDCEku1nqvP9M9v6gaX5oEqWn2lm-EwualfaksiXkvaxX7u8vhr3ve4ZCc2JnxKHgU3FHx2udfk-4f3324-0bsvH29v3t1RLwxMNASlGHqPTjGJqpFcoEOGQTTamVYE1hqvQHOvQ9cZ0ToUoDoTOtQN11Jck1fn3X0efx6wTHYXi8dh-QLHQ7GNBmG4bhdQnkGfx1IyBrvPcefybBnYk1e7tWev9uTVgrDwb__FZf-w3mF3X7qIXICXF8AV74aQXfKx3HOt5lJKtnBvzxwuNo4Rsy0-YvLYxYx-st0Y___JX5UNmWI</recordid><startdate>20060613</startdate><enddate>20060613</enddate><creator>Nishikawa, Takeshi</creator><creator>Akiyama, Nobuko</creator><creator>Kunimasa, Kazuhiro</creator><creator>Oikawa, Tsutomu</creator><creator>Ishizuka, Masaaki</creator><creator>Tsujimoto, Masafumi</creator><creator>Natori, Shunji</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060613</creationdate><title>Inhibition of in vivo angiogenesis by N-β-alanyl-5- S-glutathionyl-3,4-dihydroxyphenylalanine</title><author>Nishikawa, Takeshi ; Akiyama, Nobuko ; Kunimasa, Kazuhiro ; Oikawa, Tsutomu ; Ishizuka, Masaaki ; Tsujimoto, Masafumi ; Natori, Shunji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-ff551eccea514e56423eae1ef368a973f179c5082c8fdd937ae305d9fde862843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>5-S-GAD</topic><topic>Angiogenesis</topic><topic>Angiogenesis Inhibitors - pharmacology</topic><topic>Angiogenesis Inhibitors - therapeutic use</topic><topic>Animals</topic><topic>Antiangiogenic agent</topic><topic>Biological and medical sciences</topic><topic>Cell Line, Tumor</topic><topic>Chick Embryo</topic><topic>Dihydroxyphenylalanine - analogs & derivatives</topic><topic>Dihydroxyphenylalanine - pharmacology</topic><topic>Dihydroxyphenylalanine - therapeutic use</topic><topic>Dose-Response Relationship, Drug</topic><topic>Female</topic><topic>Glutathione - analogs & derivatives</topic><topic>Glutathione - pharmacology</topic><topic>Glutathione - therapeutic use</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Inbred ICR</topic><topic>Neovascularization, Pathologic - drug therapy</topic><topic>Neovascularization, Pathologic - pathology</topic><topic>Neovascularization, Physiologic - drug effects</topic><topic>Neovascularization, Physiologic - physiology</topic><topic>Pharmacology. Drug treatments</topic><topic>Tumor therapy</topic><topic>Xenograft Model Antitumor Assays - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nishikawa, Takeshi</creatorcontrib><creatorcontrib>Akiyama, Nobuko</creatorcontrib><creatorcontrib>Kunimasa, Kazuhiro</creatorcontrib><creatorcontrib>Oikawa, Tsutomu</creatorcontrib><creatorcontrib>Ishizuka, Masaaki</creatorcontrib><creatorcontrib>Tsujimoto, Masafumi</creatorcontrib><creatorcontrib>Natori, Shunji</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nishikawa, Takeshi</au><au>Akiyama, Nobuko</au><au>Kunimasa, Kazuhiro</au><au>Oikawa, Tsutomu</au><au>Ishizuka, Masaaki</au><au>Tsujimoto, Masafumi</au><au>Natori, Shunji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of in vivo angiogenesis by N-β-alanyl-5- S-glutathionyl-3,4-dihydroxyphenylalanine</atitle><jtitle>European journal of pharmacology</jtitle><addtitle>Eur J Pharmacol</addtitle><date>2006-06-13</date><risdate>2006</risdate><volume>539</volume><issue>3</issue><spage>151</spage><epage>157</epage><pages>151-157</pages><issn>0014-2999</issn><eissn>1879-0712</eissn><coden>EJPHAZ</coden><abstract>N-β-alanyl-5-
S-glutathionyl-3,4-dihydroxyphenylalanine (5-S-GAD), an antibacterial substance isolated from the flesh fly, inhibits human tumor growth in the nude mice model; however, the mechanism of its action is unclear. The in vivo antitumor effect includes the inhibition of tumor cell proliferation and suppression of angiogenesis. Angiogenesis is essential for tumor growth in vivo. In this study, we examined whether 5-S-GAD inhibits tumor cell-induced angiogenesis by performing the mouse dorsal air sac assay. We found that intraperitoneal administration of 5-S-GAD inhibited the angiogenesis induced by S180 mouse sarcoma cells. Furthermore, 5-S-GAD also inhibited vascular endothelial growth factor-induced angiogenesis in the Matrigel plug assay and embryonic angiogenesis in the chick embryo chorioallantoic membrane assay. However, 5-S-GAD did not show any effect on the proliferation, migration, and tube formation of vascular endothelial cells. These results provide the first evidence that a bioactive substance derived from the flesh fly has antiangiogenic activity in vivo, although the mechanisms involved could not be explained.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>16725138</pmid><doi>10.1016/j.ejphar.2006.03.084</doi><tpages>7</tpages></addata></record> |
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| subjects | 5-S-GAD Angiogenesis Angiogenesis Inhibitors - pharmacology Angiogenesis Inhibitors - therapeutic use Animals Antiangiogenic agent Biological and medical sciences Cell Line, Tumor Chick Embryo Dihydroxyphenylalanine - analogs & derivatives Dihydroxyphenylalanine - pharmacology Dihydroxyphenylalanine - therapeutic use Dose-Response Relationship, Drug Female Glutathione - analogs & derivatives Glutathione - pharmacology Glutathione - therapeutic use Humans Medical sciences Mice Mice, Inbred C57BL Mice, Inbred ICR Neovascularization, Pathologic - drug therapy Neovascularization, Pathologic - pathology Neovascularization, Physiologic - drug effects Neovascularization, Physiologic - physiology Pharmacology. Drug treatments Tumor therapy Xenograft Model Antitumor Assays - methods |
| title | Inhibition of in vivo angiogenesis by N-β-alanyl-5- S-glutathionyl-3,4-dihydroxyphenylalanine |
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