Determination of the big head carp myofibrillar ( Aristichthys nobilis) adenosine triphosphatase activity by ion chromatography

A method for the determination of adenosine triphosphatase (ATPase) activity of myofibrils of big head carp by using ion chromatography was introduced. Adenosine triphosphate (ATP), adenosine diphosphate (ADP) and orthophosphate (Pi) were separated completely. Recoveries for ATP, ADP and Pi were 98...

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Veröffentlicht in:Journal of Chromatography A 2006-06, Vol.1118 (2), p.278-280
Hauptverfasser: Gao, Rui-chang, Xue, Chang-hu, Yuan, Li, Li, Zhao-jie, Xue, Yong, Cui, Fengxia, Sun, Yan
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container_end_page 280
container_issue 2
container_start_page 278
container_title Journal of Chromatography A
container_volume 1118
creator Gao, Rui-chang
Xue, Chang-hu
Yuan, Li
Li, Zhao-jie
Xue, Yong
Cui, Fengxia
Sun, Yan
description A method for the determination of adenosine triphosphatase (ATPase) activity of myofibrils of big head carp by using ion chromatography was introduced. Adenosine triphosphate (ATP), adenosine diphosphate (ADP) and orthophosphate (Pi) were separated completely. Recoveries for ATP, ADP and Pi were 98 ± 5%, 97 ± 4% and 98 ± 5%, respectively. Pi liberated from ATP during reaction was monitored by ion chromatography using the suggested method. This method was applicable to the determination of myofibrils ATPase activity for quick quality evaluation of surimi.
doi_str_mv 10.1016/j.chroma.2006.04.066
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subjects Adenosine Triphosphatases - metabolism
Adenosine Triphosphate - metabolism
Animals
ATPase activity
Biological and medical sciences
Calibration
Carps
Chromatography, Liquid - methods
Fish and seafood industries
Food industries
Fundamental and applied biological sciences. Psychology
Hydrolysis
Ion chromatography
Myofibrils
Reproducibility of Results
Surimi quality
title Determination of the big head carp myofibrillar ( Aristichthys nobilis) adenosine triphosphatase activity by ion chromatography
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