An internal ribosome entry site promotes translation of a novel SIV Pr55(Gag) isoform
In complex retroviruses including simian immunodeficiency virus (SIV) and human immunodeficiency virus type 1 (HIV-1), the major structural proteins are encoded by the gag gene and translated as a precursor polyprotein, Pr55(Gag). An internal ribosome entry site (IRES) within the coding region of HI...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 2006-06, Vol.349 (2), p.325-334 |
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| container_title | Virology (New York, N.Y.) |
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| creator | Nicholson, Michael G Rue, Sarah M Clements, Janice E Barber, Sheila A |
| description | In complex retroviruses including simian immunodeficiency virus (SIV) and human immunodeficiency virus type 1 (HIV-1), the major structural proteins are encoded by the gag gene and translated as a precursor polyprotein, Pr55(Gag). An internal ribosome entry site (IRES) within the coding region of HIV-1 and HIV type 2 (HIV-2) gag RNA mediates expression of N-terminally truncated isoforms of the precursor polyprotein. In this study, we identify an N-terminally truncated SIV Pr55(Gag) isoform expressed from the SIV gag gene SIV p43. We demonstrate that translation of p43 occurs independently of Pr55(Gag) translation and initiates at an in-frame AUG within the gag transcript. We test several mechanisms that could mediate translation of p43 and report that translation of SIV p43 is driven by an IRES located entirely within the coding region of gag mRNA. Additionally, we present data that suggest SIV p43 affects viral replication in cell culture. |
| doi_str_mv | 10.1016/j.virol.2006.01.034 |
| format | Article |
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An internal ribosome entry site (IRES) within the coding region of HIV-1 and HIV type 2 (HIV-2) gag RNA mediates expression of N-terminally truncated isoforms of the precursor polyprotein. In this study, we identify an N-terminally truncated SIV Pr55(Gag) isoform expressed from the SIV gag gene SIV p43. We demonstrate that translation of p43 occurs independently of Pr55(Gag) translation and initiates at an in-frame AUG within the gag transcript. We test several mechanisms that could mediate translation of p43 and report that translation of SIV p43 is driven by an IRES located entirely within the coding region of gag mRNA. 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Additionally, we present data that suggest SIV p43 affects viral replication in cell culture.</description><subject>Animals</subject><subject>Cell Line</subject><subject>Cercopithecus aethiops</subject><subject>Codon, Initiator</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Gene Products, gag - biosynthesis</subject><subject>Humans</subject><subject>Immunoprecipitation</subject><subject>Protein Biosynthesis</subject><subject>Protein Isoforms - biosynthesis</subject><subject>Protein Precursors - biosynthesis</subject><subject>Retroviridae Proteins - biosynthesis</subject><subject>Ribosomes - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA, Viral - genetics</subject><subject>RNA, Viral - metabolism</subject><subject>Simian Immunodeficiency Virus - genetics</subject><subject>Simian Immunodeficiency Virus - physiology</subject><issn>0042-6822</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kE1Lw0AYhPeg2Fr9BYLsSfSQ-O5HssmxlFoLBQWt17BJ3siWZLfubgv99wasp2HgYZgZQu4YpAxY_rxLj8a7PuUAeQosBSEvyBRA8iQvOJ-Q6xB2MHql4IpMWC5LWTI5Jdu5pcZG9Fb31JvaBTcgRRv9iQYTke69G1zEQKPXNvQ6Gmep66im1h2xpx_rL_rus-xxpb-fqAmuc364IZed7gPennVGti_Lz8VrsnlbrRfzTbJnXMakLlTJVNmAqutMt4BYYFeonKlMNEqJrsllV7ZlwduSIx-HAIMCsM2UaOpOihl5-MsdW_4cMMRqMKHBvtcW3SFUeQFCSQUjeH8GD_WAbbX3ZtD-VP0fIX4BV45ezQ</recordid><startdate>20060605</startdate><enddate>20060605</enddate><creator>Nicholson, Michael G</creator><creator>Rue, Sarah M</creator><creator>Clements, Janice E</creator><creator>Barber, Sheila A</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20060605</creationdate><title>An internal ribosome entry site promotes translation of a novel SIV Pr55(Gag) isoform</title><author>Nicholson, Michael G ; Rue, Sarah M ; Clements, Janice E ; Barber, Sheila A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p124t-b879179c07bb5ad0ee8ef8761753c773fc64f9d982d92e282201080ed573cbf43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Cell Line</topic><topic>Cercopithecus aethiops</topic><topic>Codon, Initiator</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Gene Products, gag - biosynthesis</topic><topic>Humans</topic><topic>Immunoprecipitation</topic><topic>Protein Biosynthesis</topic><topic>Protein Isoforms - biosynthesis</topic><topic>Protein Precursors - biosynthesis</topic><topic>Retroviridae Proteins - biosynthesis</topic><topic>Ribosomes - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>RNA, Viral - genetics</topic><topic>RNA, Viral - metabolism</topic><topic>Simian Immunodeficiency Virus - genetics</topic><topic>Simian Immunodeficiency Virus - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nicholson, Michael G</creatorcontrib><creatorcontrib>Rue, Sarah M</creatorcontrib><creatorcontrib>Clements, Janice E</creatorcontrib><creatorcontrib>Barber, Sheila A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nicholson, Michael G</au><au>Rue, Sarah M</au><au>Clements, Janice E</au><au>Barber, Sheila A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An internal ribosome entry site promotes translation of a novel SIV Pr55(Gag) isoform</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>2006-06-05</date><risdate>2006</risdate><volume>349</volume><issue>2</issue><spage>325</spage><epage>334</epage><pages>325-334</pages><issn>0042-6822</issn><abstract>In complex retroviruses including simian immunodeficiency virus (SIV) and human immunodeficiency virus type 1 (HIV-1), the major structural proteins are encoded by the gag gene and translated as a precursor polyprotein, Pr55(Gag). 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| identifier | ISSN: 0042-6822 |
| ispartof | Virology (New York, N.Y.), 2006-06, Vol.349 (2), p.325-334 |
| issn | 0042-6822 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Cell Line Cercopithecus aethiops Codon, Initiator Electrophoresis, Polyacrylamide Gel Gene Products, gag - biosynthesis Humans Immunoprecipitation Protein Biosynthesis Protein Isoforms - biosynthesis Protein Precursors - biosynthesis Retroviridae Proteins - biosynthesis Ribosomes - metabolism RNA, Messenger - metabolism RNA, Viral - genetics RNA, Viral - metabolism Simian Immunodeficiency Virus - genetics Simian Immunodeficiency Virus - physiology |
| title | An internal ribosome entry site promotes translation of a novel SIV Pr55(Gag) isoform |
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