A novel electroporation device for gene delivery in large animals and humans
Intramuscular injection of plasmid DNA followed by electrical stimulation (electroporation) is an efficient method for achieving therapeutic levels of encoded proteins or eliciting efficient immune responses in smaller animals such as mice and rats. Electroporation in larger animals and humans poses...
Gespeichert in:
| Veröffentlicht in: | Vaccine 2006-05, Vol.24 (21), p.4667-4670 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 4670 |
|---|---|
| container_issue | 21 |
| container_start_page | 4667 |
| container_title | Vaccine |
| container_volume | 24 |
| creator | Tjelle, Torunn Elisabeth Salte, Ragnar Mathiesen, Iacob Kjeken, Rune |
| description | Intramuscular injection of plasmid DNA followed by electrical stimulation (electroporation) is an efficient method for achieving therapeutic levels of encoded proteins or eliciting efficient immune responses in smaller animals such as mice and rats. Electroporation in larger animals and humans poses new technical challenges, the main difficulty being to maintain efficacy while limiting invasiveness and pain.
Here we present data using a new device for combined injection and electroporation in large animals and humans. The device injects DNA through two needles during insertion into the muscle and thus distributes the injection volume along the needles which also serve as electrodes. Since the electrical field is strongest close to the needle-electrode, a near perfect match between the DNA and the electric field is achieved. We show that using moderate amounts of DNA: (1) muscle tissue is transfected along the entire length of the needle path, (2) the efficacy is higher compared to when the DNA is injected between the electrodes, (3) level of protein expression can be tightly controlled by the number of treatments, and (4) efficient immunization is achieved. |
| doi_str_mv | 10.1016/j.vaccine.2005.08.068 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68035937</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0264410X05008431</els_id><sourcerecordid>68035937</sourcerecordid><originalsourceid>FETCH-LOGICAL-c422t-ad9e2b44a016f4854bbb8fd927eb35718b4f1e282feaeb82cbeda66e221d571b3</originalsourceid><addsrcrecordid>eNqFkU1LJDEQQIOs6Kz6E5TAgrduk3Q6nT6JiO4KA14UvIV8VGuGnmRMphv890ZmYMGLp6IqrypUPYTOKakpoeJqVc_aWh-gZoS0NZE1EfIALajsmoq1VP5CC8IErzglL8fod84rUsCG9kfomAoqWCO6BVre4BBnGDGMYLcpbmLSWx8DdjB7C3iICb9CgJKPfob0gX3Ao06vgHXwaz3mEh1-m9Y65FN0OJQKnO3jCXq-v3u6_VctH_8-3N4sK8sZ21ba9cAM57rsMXDZcmOMHFzPOjBN21Fp-ECBSTaABiOZNeC0EMAYdeXZNCfocjd3k-L7BHmr1j5bGEcdIE5ZCUmatm-6H0HaMUp4zwv45xu4ilMKZQlF27YnRHQdK1S7o2yKOScY1CaVG6QPRYn6sqJWam9FfVlRRKpipfRd7KdPZg3uf9deQwGudwCUq80eksrWQ7DgfCpalIv-hy8-AS2AoFg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1559006772</pqid></control><display><type>article</type><title>A novel electroporation device for gene delivery in large animals and humans</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Tjelle, Torunn Elisabeth ; Salte, Ragnar ; Mathiesen, Iacob ; Kjeken, Rune</creator><creatorcontrib>Tjelle, Torunn Elisabeth ; Salte, Ragnar ; Mathiesen, Iacob ; Kjeken, Rune</creatorcontrib><description>Intramuscular injection of plasmid DNA followed by electrical stimulation (electroporation) is an efficient method for achieving therapeutic levels of encoded proteins or eliciting efficient immune responses in smaller animals such as mice and rats. Electroporation in larger animals and humans poses new technical challenges, the main difficulty being to maintain efficacy while limiting invasiveness and pain.
Here we present data using a new device for combined injection and electroporation in large animals and humans. The device injects DNA through two needles during insertion into the muscle and thus distributes the injection volume along the needles which also serve as electrodes. Since the electrical field is strongest close to the needle-electrode, a near perfect match between the DNA and the electric field is achieved. We show that using moderate amounts of DNA: (1) muscle tissue is transfected along the entire length of the needle path, (2) the efficacy is higher compared to when the DNA is injected between the electrodes, (3) level of protein expression can be tightly controlled by the number of treatments, and (4) efficient immunization is achieved.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2005.08.068</identifier><identifier>PMID: 16162367</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Animals ; Antibody Formation ; beta-Galactosidase - genetics ; Deoxyribonucleic acid ; Design ; DNA ; DNA vaccination ; Efficiency ; Electrodes ; Electroporation ; Electroporation - instrumentation ; Equipment Design ; Gene expression ; Gene Transfer Techniques ; Genetic Therapy ; Green Fluorescent Proteins - genetics ; Humans ; Immunization ; Injection ; Mice ; Needles ; Pain ; Rats ; Sheep</subject><ispartof>Vaccine, 2006-05, Vol.24 (21), p.4667-4670</ispartof><rights>2005 Elsevier Ltd</rights><rights>Copyright Elsevier Limited May 22, 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-ad9e2b44a016f4854bbb8fd927eb35718b4f1e282feaeb82cbeda66e221d571b3</citedby><cites>FETCH-LOGICAL-c422t-ad9e2b44a016f4854bbb8fd927eb35718b4f1e282feaeb82cbeda66e221d571b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0264410X05008431$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16162367$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tjelle, Torunn Elisabeth</creatorcontrib><creatorcontrib>Salte, Ragnar</creatorcontrib><creatorcontrib>Mathiesen, Iacob</creatorcontrib><creatorcontrib>Kjeken, Rune</creatorcontrib><title>A novel electroporation device for gene delivery in large animals and humans</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Intramuscular injection of plasmid DNA followed by electrical stimulation (electroporation) is an efficient method for achieving therapeutic levels of encoded proteins or eliciting efficient immune responses in smaller animals such as mice and rats. Electroporation in larger animals and humans poses new technical challenges, the main difficulty being to maintain efficacy while limiting invasiveness and pain.
Here we present data using a new device for combined injection and electroporation in large animals and humans. The device injects DNA through two needles during insertion into the muscle and thus distributes the injection volume along the needles which also serve as electrodes. Since the electrical field is strongest close to the needle-electrode, a near perfect match between the DNA and the electric field is achieved. We show that using moderate amounts of DNA: (1) muscle tissue is transfected along the entire length of the needle path, (2) the efficacy is higher compared to when the DNA is injected between the electrodes, (3) level of protein expression can be tightly controlled by the number of treatments, and (4) efficient immunization is achieved.</description><subject>Animals</subject><subject>Antibody Formation</subject><subject>beta-Galactosidase - genetics</subject><subject>Deoxyribonucleic acid</subject><subject>Design</subject><subject>DNA</subject><subject>DNA vaccination</subject><subject>Efficiency</subject><subject>Electrodes</subject><subject>Electroporation</subject><subject>Electroporation - instrumentation</subject><subject>Equipment Design</subject><subject>Gene expression</subject><subject>Gene Transfer Techniques</subject><subject>Genetic Therapy</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>Humans</subject><subject>Immunization</subject><subject>Injection</subject><subject>Mice</subject><subject>Needles</subject><subject>Pain</subject><subject>Rats</subject><subject>Sheep</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkU1LJDEQQIOs6Kz6E5TAgrduk3Q6nT6JiO4KA14UvIV8VGuGnmRMphv890ZmYMGLp6IqrypUPYTOKakpoeJqVc_aWh-gZoS0NZE1EfIALajsmoq1VP5CC8IErzglL8fod84rUsCG9kfomAoqWCO6BVre4BBnGDGMYLcpbmLSWx8DdjB7C3iICb9CgJKPfob0gX3Ao06vgHXwaz3mEh1-m9Y65FN0OJQKnO3jCXq-v3u6_VctH_8-3N4sK8sZ21ba9cAM57rsMXDZcmOMHFzPOjBN21Fp-ECBSTaABiOZNeC0EMAYdeXZNCfocjd3k-L7BHmr1j5bGEcdIE5ZCUmatm-6H0HaMUp4zwv45xu4ilMKZQlF27YnRHQdK1S7o2yKOScY1CaVG6QPRYn6sqJWam9FfVlRRKpipfRd7KdPZg3uf9deQwGudwCUq80eksrWQ7DgfCpalIv-hy8-AS2AoFg</recordid><startdate>20060522</startdate><enddate>20060522</enddate><creator>Tjelle, Torunn Elisabeth</creator><creator>Salte, Ragnar</creator><creator>Mathiesen, Iacob</creator><creator>Kjeken, Rune</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20060522</creationdate><title>A novel electroporation device for gene delivery in large animals and humans</title><author>Tjelle, Torunn Elisabeth ; Salte, Ragnar ; Mathiesen, Iacob ; Kjeken, Rune</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-ad9e2b44a016f4854bbb8fd927eb35718b4f1e282feaeb82cbeda66e221d571b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Antibody Formation</topic><topic>beta-Galactosidase - genetics</topic><topic>Deoxyribonucleic acid</topic><topic>Design</topic><topic>DNA</topic><topic>DNA vaccination</topic><topic>Efficiency</topic><topic>Electrodes</topic><topic>Electroporation</topic><topic>Electroporation - instrumentation</topic><topic>Equipment Design</topic><topic>Gene expression</topic><topic>Gene Transfer Techniques</topic><topic>Genetic Therapy</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>Humans</topic><topic>Immunization</topic><topic>Injection</topic><topic>Mice</topic><topic>Needles</topic><topic>Pain</topic><topic>Rats</topic><topic>Sheep</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tjelle, Torunn Elisabeth</creatorcontrib><creatorcontrib>Salte, Ragnar</creatorcontrib><creatorcontrib>Mathiesen, Iacob</creatorcontrib><creatorcontrib>Kjeken, Rune</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nursing & Allied Health Database</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Healthcare Administration Database (Alumni)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Healthcare Administration Database</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tjelle, Torunn Elisabeth</au><au>Salte, Ragnar</au><au>Mathiesen, Iacob</au><au>Kjeken, Rune</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel electroporation device for gene delivery in large animals and humans</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2006-05-22</date><risdate>2006</risdate><volume>24</volume><issue>21</issue><spage>4667</spage><epage>4670</epage><pages>4667-4670</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><abstract>Intramuscular injection of plasmid DNA followed by electrical stimulation (electroporation) is an efficient method for achieving therapeutic levels of encoded proteins or eliciting efficient immune responses in smaller animals such as mice and rats. Electroporation in larger animals and humans poses new technical challenges, the main difficulty being to maintain efficacy while limiting invasiveness and pain.
Here we present data using a new device for combined injection and electroporation in large animals and humans. The device injects DNA through two needles during insertion into the muscle and thus distributes the injection volume along the needles which also serve as electrodes. Since the electrical field is strongest close to the needle-electrode, a near perfect match between the DNA and the electric field is achieved. We show that using moderate amounts of DNA: (1) muscle tissue is transfected along the entire length of the needle path, (2) the efficacy is higher compared to when the DNA is injected between the electrodes, (3) level of protein expression can be tightly controlled by the number of treatments, and (4) efficient immunization is achieved.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>16162367</pmid><doi>10.1016/j.vaccine.2005.08.068</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0264-410X |
| ispartof | Vaccine, 2006-05, Vol.24 (21), p.4667-4670 |
| issn | 0264-410X 1873-2518 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68035937 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Antibody Formation beta-Galactosidase - genetics Deoxyribonucleic acid Design DNA DNA vaccination Efficiency Electrodes Electroporation Electroporation - instrumentation Equipment Design Gene expression Gene Transfer Techniques Genetic Therapy Green Fluorescent Proteins - genetics Humans Immunization Injection Mice Needles Pain Rats Sheep |
| title | A novel electroporation device for gene delivery in large animals and humans |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T08%3A17%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20novel%20electroporation%20device%20for%20gene%20delivery%20in%20large%20animals%20and%20humans&rft.jtitle=Vaccine&rft.au=Tjelle,%20Torunn%20Elisabeth&rft.date=2006-05-22&rft.volume=24&rft.issue=21&rft.spage=4667&rft.epage=4670&rft.pages=4667-4670&rft.issn=0264-410X&rft.eissn=1873-2518&rft_id=info:doi/10.1016/j.vaccine.2005.08.068&rft_dat=%3Cproquest_cross%3E68035937%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1559006772&rft_id=info:pmid/16162367&rft_els_id=S0264410X05008431&rfr_iscdi=true |