Feasibility of in vivo dual-energy myocardial SPECT for monitoring the distribution of transplanted cells in relation to the infarction site
Cell therapy using bone marrow mesenchymal stem cells (BMSCs) shows promise in the treatment of myocardial infarction (MI) but accurate cell delivery within MI areas remains critical. In the present study, we tested the feasibility of in vivo pinhole SPECT imaging for monitoring the sites of intramy...
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| Veröffentlicht in: | European journal of nuclear medicine and molecular imaging 2006-06, Vol.33 (6), p.709-715 |
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| container_title | European journal of nuclear medicine and molecular imaging |
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| creator | Tran, Nguyen Poussier, Sylvain Franken, Philippe R Maskali, Fatiha Groubatch, Frederique Vanhove, Chris Antunes, Laurent Karcher, Gilles Villemot, Jean-Pierre Marie, Pierre-Yves |
| description | Cell therapy using bone marrow mesenchymal stem cells (BMSCs) shows promise in the treatment of myocardial infarction (MI) but accurate cell delivery within MI areas remains critical. In the present study, we tested the feasibility of in vivo pinhole SPECT imaging for monitoring the sites of intramyocardial implanted BMSCs in relation to targeted MI areas in rats.
BMSCs were labelled with (111)In-oxine and injected within the fibrotic areas of 3-month-old MI in ten rats. Two days later, dual (111)In/(99m)Tc-sestamibi pinhole SPECT was recorded for localisation of (111)In-BMSCs on a 15-segment left ventricular (LV) division. Additional (99m)Tc-sestamibi pinhole SPECT had been performed 1 month earlier and on the day before transplantation. In vitro counting on histological sections was used to validate the pinhole SPECT determination of (111)In-BMSC activity within LV segments.
The underperfused MI area (segments with |
| doi_str_mv | 10.1007/s00259-006-0075-9 |
| format | Article |
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BMSCs were labelled with (111)In-oxine and injected within the fibrotic areas of 3-month-old MI in ten rats. Two days later, dual (111)In/(99m)Tc-sestamibi pinhole SPECT was recorded for localisation of (111)In-BMSCs on a 15-segment left ventricular (LV) division. Additional (99m)Tc-sestamibi pinhole SPECT had been performed 1 month earlier and on the day before transplantation. In vitro counting on histological sections was used to validate the pinhole SPECT determination of (111)In-BMSC activity within LV segments.
The underperfused MI area (segments with <70% uptake) was stable between the (99m)Tc-sestamibi SPECT study recorded at 1 month (4.6+/-1.9 segments) and at 1 day (4.7+/-2.3 segments) before transplantation. (111)In-BMSCs were detected by dual-energy SPECT in 56 segments: 33 (59%) were underperfused MI segments but 23 (41%) were not (14 adjacent and nine remote segments). Finally, (111)In-labelled BMSCs were not detected in 14 out of the 47 (30%) underperfused MI segments.
When BMSCs are injected within MI areas in rats, sites of early cell retention do not always match the targeted MI areas. The dual-energy pinhole SPECT technique may be used for monitoring the sites of early retention of implanted BMSCs and the data obtained may have critical importance when analysing the effects of cardiac cell therapy.</description><identifier>ISSN: 1619-7070</identifier><identifier>EISSN: 1619-7089</identifier><identifier>DOI: 10.1007/s00259-006-0075-9</identifier><identifier>PMID: 16572303</identifier><language>eng</language><publisher>Germany: Springer Nature B.V</publisher><subject>Animals ; Feasibility Studies ; Image Enhancement - methods ; Male ; Mesenchymal Stem Cell Transplantation - methods ; Mesenchymal Stromal Cells - diagnostic imaging ; Myocardial Infarction - diagnostic imaging ; Myocardial Infarction - surgery ; Rats ; Rats, Wistar ; Tomography, Emission-Computed, Single-Photon - methods ; Treatment Outcome</subject><ispartof>European journal of nuclear medicine and molecular imaging, 2006-06, Vol.33 (6), p.709-715</ispartof><rights>Springer-Verlag 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c326t-42153a829ae1d5a9d19d413aaabf3b239b17a78057386cdb6c3939150c0b13463</citedby><cites>FETCH-LOGICAL-c326t-42153a829ae1d5a9d19d413aaabf3b239b17a78057386cdb6c3939150c0b13463</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16572303$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tran, Nguyen</creatorcontrib><creatorcontrib>Poussier, Sylvain</creatorcontrib><creatorcontrib>Franken, Philippe R</creatorcontrib><creatorcontrib>Maskali, Fatiha</creatorcontrib><creatorcontrib>Groubatch, Frederique</creatorcontrib><creatorcontrib>Vanhove, Chris</creatorcontrib><creatorcontrib>Antunes, Laurent</creatorcontrib><creatorcontrib>Karcher, Gilles</creatorcontrib><creatorcontrib>Villemot, Jean-Pierre</creatorcontrib><creatorcontrib>Marie, Pierre-Yves</creatorcontrib><title>Feasibility of in vivo dual-energy myocardial SPECT for monitoring the distribution of transplanted cells in relation to the infarction site</title><title>European journal of nuclear medicine and molecular imaging</title><addtitle>Eur J Nucl Med Mol Imaging</addtitle><description>Cell therapy using bone marrow mesenchymal stem cells (BMSCs) shows promise in the treatment of myocardial infarction (MI) but accurate cell delivery within MI areas remains critical. In the present study, we tested the feasibility of in vivo pinhole SPECT imaging for monitoring the sites of intramyocardial implanted BMSCs in relation to targeted MI areas in rats.
BMSCs were labelled with (111)In-oxine and injected within the fibrotic areas of 3-month-old MI in ten rats. Two days later, dual (111)In/(99m)Tc-sestamibi pinhole SPECT was recorded for localisation of (111)In-BMSCs on a 15-segment left ventricular (LV) division. Additional (99m)Tc-sestamibi pinhole SPECT had been performed 1 month earlier and on the day before transplantation. In vitro counting on histological sections was used to validate the pinhole SPECT determination of (111)In-BMSC activity within LV segments.
The underperfused MI area (segments with <70% uptake) was stable between the (99m)Tc-sestamibi SPECT study recorded at 1 month (4.6+/-1.9 segments) and at 1 day (4.7+/-2.3 segments) before transplantation. (111)In-BMSCs were detected by dual-energy SPECT in 56 segments: 33 (59%) were underperfused MI segments but 23 (41%) were not (14 adjacent and nine remote segments). Finally, (111)In-labelled BMSCs were not detected in 14 out of the 47 (30%) underperfused MI segments.
When BMSCs are injected within MI areas in rats, sites of early cell retention do not always match the targeted MI areas. The dual-energy pinhole SPECT technique may be used for monitoring the sites of early retention of implanted BMSCs and the data obtained may have critical importance when analysing the effects of cardiac cell therapy.</description><subject>Animals</subject><subject>Feasibility Studies</subject><subject>Image Enhancement - methods</subject><subject>Male</subject><subject>Mesenchymal Stem Cell Transplantation - methods</subject><subject>Mesenchymal Stromal Cells - diagnostic imaging</subject><subject>Myocardial Infarction - diagnostic imaging</subject><subject>Myocardial Infarction - surgery</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Tomography, Emission-Computed, Single-Photon - methods</subject><subject>Treatment Outcome</subject><issn>1619-7070</issn><issn>1619-7089</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkcFq3DAQhkVJadK0D5BLEDnk5nYkWbJ1LEvSFgItND2LsSWnCra0keTAvkMfOvbukkIPgwbx_T8DHyEXDD4xgOZzBuBSVwBqmUZW-g05Y4rpqoFWn7zuDZyS9zk_ArCWt_odOWVKNlyAOCN_bx1m3_nRlx2NA_WBPvvnSO2MY-WCSw87Ou1ij8l6HOmvnzebezrERKcYfInJhwda_jhqfS7Jd3PxMaw9JWHI2xFDcZb2bhzzWp3ciHuixH3KhwFTv__JvrgP5O2AY3Yfj-85-X17c7_5Vt39-Pp98-Wu6gVXpao5kwJbrtExK1Fbpm3NBCJ2g-i40B1rsGlBNqJVve1UL7TQTEIPHRO1Eufk-tC7TfFpdrmYyef1SAwuztmoFoRUDBbw6j_wMc4pLLcZzmqluKxXiB2gPsWckxvMNvkJ084wMKsnc_BkFk9m9WT0krk8Fs_d5Oy_xFGMeAF51Y87</recordid><startdate>200606</startdate><enddate>200606</enddate><creator>Tran, Nguyen</creator><creator>Poussier, Sylvain</creator><creator>Franken, Philippe R</creator><creator>Maskali, Fatiha</creator><creator>Groubatch, Frederique</creator><creator>Vanhove, Chris</creator><creator>Antunes, Laurent</creator><creator>Karcher, Gilles</creator><creator>Villemot, Jean-Pierre</creator><creator>Marie, Pierre-Yves</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>200606</creationdate><title>Feasibility of in vivo dual-energy myocardial SPECT for monitoring the distribution of transplanted cells in relation to the infarction site</title><author>Tran, Nguyen ; Poussier, Sylvain ; Franken, Philippe R ; Maskali, Fatiha ; Groubatch, Frederique ; Vanhove, Chris ; Antunes, Laurent ; Karcher, Gilles ; Villemot, Jean-Pierre ; Marie, Pierre-Yves</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c326t-42153a829ae1d5a9d19d413aaabf3b239b17a78057386cdb6c3939150c0b13463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Feasibility Studies</topic><topic>Image Enhancement - 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Academic</collection><jtitle>European journal of nuclear medicine and molecular imaging</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tran, Nguyen</au><au>Poussier, Sylvain</au><au>Franken, Philippe R</au><au>Maskali, Fatiha</au><au>Groubatch, Frederique</au><au>Vanhove, Chris</au><au>Antunes, Laurent</au><au>Karcher, Gilles</au><au>Villemot, Jean-Pierre</au><au>Marie, Pierre-Yves</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Feasibility of in vivo dual-energy myocardial SPECT for monitoring the distribution of transplanted cells in relation to the infarction site</atitle><jtitle>European journal of nuclear medicine and molecular imaging</jtitle><addtitle>Eur J Nucl Med Mol Imaging</addtitle><date>2006-06</date><risdate>2006</risdate><volume>33</volume><issue>6</issue><spage>709</spage><epage>715</epage><pages>709-715</pages><issn>1619-7070</issn><eissn>1619-7089</eissn><abstract>Cell therapy using bone marrow mesenchymal stem cells (BMSCs) shows promise in the treatment of myocardial infarction (MI) but accurate cell delivery within MI areas remains critical. In the present study, we tested the feasibility of in vivo pinhole SPECT imaging for monitoring the sites of intramyocardial implanted BMSCs in relation to targeted MI areas in rats.
BMSCs were labelled with (111)In-oxine and injected within the fibrotic areas of 3-month-old MI in ten rats. Two days later, dual (111)In/(99m)Tc-sestamibi pinhole SPECT was recorded for localisation of (111)In-BMSCs on a 15-segment left ventricular (LV) division. Additional (99m)Tc-sestamibi pinhole SPECT had been performed 1 month earlier and on the day before transplantation. In vitro counting on histological sections was used to validate the pinhole SPECT determination of (111)In-BMSC activity within LV segments.
The underperfused MI area (segments with <70% uptake) was stable between the (99m)Tc-sestamibi SPECT study recorded at 1 month (4.6+/-1.9 segments) and at 1 day (4.7+/-2.3 segments) before transplantation. (111)In-BMSCs were detected by dual-energy SPECT in 56 segments: 33 (59%) were underperfused MI segments but 23 (41%) were not (14 adjacent and nine remote segments). Finally, (111)In-labelled BMSCs were not detected in 14 out of the 47 (30%) underperfused MI segments.
When BMSCs are injected within MI areas in rats, sites of early cell retention do not always match the targeted MI areas. The dual-energy pinhole SPECT technique may be used for monitoring the sites of early retention of implanted BMSCs and the data obtained may have critical importance when analysing the effects of cardiac cell therapy.</abstract><cop>Germany</cop><pub>Springer Nature B.V</pub><pmid>16572303</pmid><doi>10.1007/s00259-006-0075-9</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Feasibility Studies Image Enhancement - methods Male Mesenchymal Stem Cell Transplantation - methods Mesenchymal Stromal Cells - diagnostic imaging Myocardial Infarction - diagnostic imaging Myocardial Infarction - surgery Rats Rats, Wistar Tomography, Emission-Computed, Single-Photon - methods Treatment Outcome |
| title | Feasibility of in vivo dual-energy myocardial SPECT for monitoring the distribution of transplanted cells in relation to the infarction site |
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