Procolipase gene expression in the rat brain: Source of endogenous enterostatin production in the brain
Enterostatin is a pentapeptide released from its precursor protein procolipase, which is synthesized in the exocrine pancreas and gastric mucosa. As central injection of enterostatin has potent effects on feeding, we hypothesized that the procolipase may also be expressed in the brain. We confirmed...
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| Veröffentlicht in: | Brain research 2006-05, Vol.1087 (1), p.52-59 |
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| creator | York, David A. Lin, Ling Thomas, Sonjya R. Braymer, Hugh D. Park, MieJung |
| description | Enterostatin is a pentapeptide released from its precursor protein procolipase, which is synthesized in the exocrine pancreas and gastric mucosa. As central injection of enterostatin has potent effects on feeding, we hypothesized that the procolipase may also be expressed in the brain. We confirmed the presence of preprocolipase gene expression in amygdala by reverse transcription-polymerase chain reaction and Northern blot analysis and of protein expression by Western blots. Immunohistochemical analysis using antibodies for procolipase and enterostatin identified their immunoreactivity (IR) in rat brain. Procolipase IR was present in the cytoplasm of paraventricular, amygdala, and the dorsal thalamus nucleus. Enterostatin IR was evident in the fibers of the dorsal thalamus and arcuate nucleus. In vivo injection of enterostatin antibody into rat amygdala increased food intake. These data suggest that procolipase and enterostatin are synthesized within specific regions of the brain that function in the regulation of food intake centrally. |
| doi_str_mv | 10.1016/j.brainres.2006.03.013 |
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As central injection of enterostatin has potent effects on feeding, we hypothesized that the procolipase may also be expressed in the brain. We confirmed the presence of preprocolipase gene expression in amygdala by reverse transcription-polymerase chain reaction and Northern blot analysis and of protein expression by Western blots. Immunohistochemical analysis using antibodies for procolipase and enterostatin identified their immunoreactivity (IR) in rat brain. Procolipase IR was present in the cytoplasm of paraventricular, amygdala, and the dorsal thalamus nucleus. Enterostatin IR was evident in the fibers of the dorsal thalamus and arcuate nucleus. In vivo injection of enterostatin antibody into rat amygdala increased food intake. These data suggest that procolipase and enterostatin are synthesized within specific regions of the brain that function in the regulation of food intake centrally.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/j.brainres.2006.03.013</identifier><identifier>PMID: 16624258</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Amygdala ; Analysis of Variance ; Animals ; Antibodies - pharmacology ; Behavior, Animal ; Biochemistry and metabolism ; Biological and medical sciences ; Blotting, Northern - methods ; Blotting, Western - methods ; Brain - physiology ; Central nervous system ; Colipases - genetics ; Colipases - immunology ; Colipases - metabolism ; Eating - drug effects ; Eating - physiology ; Enzyme Precursors ; Fundamental and applied biological sciences. Psychology ; Gene Expression - physiology ; Hypothalamus ; Immunohistochemistry ; Immunohistochemistry - methods ; Male ; Northern blot ; Protein Precursors - genetics ; Protein Precursors - immunology ; Protein Precursors - metabolism ; Rats ; Reverse Transcriptase Polymerase Chain Reaction - methods ; RNA, Messenger - metabolism ; RT-PCR ; Vertebrates: nervous system and sense organs ; Western blot</subject><ispartof>Brain research, 2006-05, Vol.1087 (1), p.52-59</ispartof><rights>2006 Elsevier B.V.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-66650a8975e2f625d4de298df9bd029fe61f6f4eb350ce1dfdde87a4397126cf3</citedby><cites>FETCH-LOGICAL-c427t-66650a8975e2f625d4de298df9bd029fe61f6f4eb350ce1dfdde87a4397126cf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.brainres.2006.03.013$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17848005$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16624258$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>York, David A.</creatorcontrib><creatorcontrib>Lin, Ling</creatorcontrib><creatorcontrib>Thomas, Sonjya R.</creatorcontrib><creatorcontrib>Braymer, Hugh D.</creatorcontrib><creatorcontrib>Park, MieJung</creatorcontrib><title>Procolipase gene expression in the rat brain: Source of endogenous enterostatin production in the brain</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>Enterostatin is a pentapeptide released from its precursor protein procolipase, which is synthesized in the exocrine pancreas and gastric mucosa. As central injection of enterostatin has potent effects on feeding, we hypothesized that the procolipase may also be expressed in the brain. We confirmed the presence of preprocolipase gene expression in amygdala by reverse transcription-polymerase chain reaction and Northern blot analysis and of protein expression by Western blots. Immunohistochemical analysis using antibodies for procolipase and enterostatin identified their immunoreactivity (IR) in rat brain. Procolipase IR was present in the cytoplasm of paraventricular, amygdala, and the dorsal thalamus nucleus. Enterostatin IR was evident in the fibers of the dorsal thalamus and arcuate nucleus. In vivo injection of enterostatin antibody into rat amygdala increased food intake. These data suggest that procolipase and enterostatin are synthesized within specific regions of the brain that function in the regulation of food intake centrally.</description><subject>Amygdala</subject><subject>Analysis of Variance</subject><subject>Animals</subject><subject>Antibodies - pharmacology</subject><subject>Behavior, Animal</subject><subject>Biochemistry and metabolism</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern - methods</subject><subject>Blotting, Western - methods</subject><subject>Brain - physiology</subject><subject>Central nervous system</subject><subject>Colipases - genetics</subject><subject>Colipases - immunology</subject><subject>Colipases - metabolism</subject><subject>Eating - drug effects</subject><subject>Eating - physiology</subject><subject>Enzyme Precursors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression - physiology</subject><subject>Hypothalamus</subject><subject>Immunohistochemistry</subject><subject>Immunohistochemistry - methods</subject><subject>Male</subject><subject>Northern blot</subject><subject>Protein Precursors - genetics</subject><subject>Protein Precursors - immunology</subject><subject>Protein Precursors - metabolism</subject><subject>Rats</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - methods</subject><subject>RNA, Messenger - metabolism</subject><subject>RT-PCR</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>Western blot</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-PFCEQxYnRuOPqV9hw0Vu3BXTT4Emz8V-yiSbqmTBQrEx6mhHojX572Z0x621PQOr3qh71CLlg0DNg8vWu32Ybl4yl5wCyB9EDE4_IhqmJd5IP8JhsoFU6pbU4I89K2bWnEBqekjMmG8FHtSHXX3NyaY4HW5Be44IUfx9a1xLTQuNC60-k2VZ6N-0N_ZbW7JCmQHHxqfFpLe1aMadSbW2CQ05-dfU_-Z30OXkS7Fzwxek8Jz8-vP9--am7-vLx8-W7q84NfKqdlHIEq_Q0Ig-Sj37wyLXyQW89cB1QsiDDgFsxgkPmg_eoJjsIPTEuXRDn5NWxb_Pxa8VSzT4Wh_NsF2xejVQgxhGmB0E2ccY40w2UR9C1P5aMwRxy3Nv8xzAwt1mYnfmXhbnNwoAwLYsmvDhNWLd79Pey0_Ib8PIE2OLsHLJdXCz33KQGBTA27u2Rw7a4m4jZFBdxcehjRleNT_EhL38BTlmswA</recordid><startdate>20060504</startdate><enddate>20060504</enddate><creator>York, David A.</creator><creator>Lin, Ling</creator><creator>Thomas, Sonjya R.</creator><creator>Braymer, Hugh D.</creator><creator>Park, MieJung</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20060504</creationdate><title>Procolipase gene expression in the rat brain: Source of endogenous enterostatin production in the brain</title><author>York, David A. ; Lin, Ling ; Thomas, Sonjya R. ; Braymer, Hugh D. ; Park, MieJung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-66650a8975e2f625d4de298df9bd029fe61f6f4eb350ce1dfdde87a4397126cf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Amygdala</topic><topic>Analysis of Variance</topic><topic>Animals</topic><topic>Antibodies - pharmacology</topic><topic>Behavior, Animal</topic><topic>Biochemistry and metabolism</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern - methods</topic><topic>Blotting, Western - methods</topic><topic>Brain - physiology</topic><topic>Central nervous system</topic><topic>Colipases - genetics</topic><topic>Colipases - immunology</topic><topic>Colipases - metabolism</topic><topic>Eating - drug effects</topic><topic>Eating - physiology</topic><topic>Enzyme Precursors</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression - physiology</topic><topic>Hypothalamus</topic><topic>Immunohistochemistry</topic><topic>Immunohistochemistry - methods</topic><topic>Male</topic><topic>Northern blot</topic><topic>Protein Precursors - genetics</topic><topic>Protein Precursors - immunology</topic><topic>Protein Precursors - metabolism</topic><topic>Rats</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - methods</topic><topic>RNA, Messenger - metabolism</topic><topic>RT-PCR</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>Western blot</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>York, David A.</creatorcontrib><creatorcontrib>Lin, Ling</creatorcontrib><creatorcontrib>Thomas, Sonjya R.</creatorcontrib><creatorcontrib>Braymer, Hugh D.</creatorcontrib><creatorcontrib>Park, MieJung</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>York, David A.</au><au>Lin, Ling</au><au>Thomas, Sonjya R.</au><au>Braymer, Hugh D.</au><au>Park, MieJung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Procolipase gene expression in the rat brain: Source of endogenous enterostatin production in the brain</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>2006-05-04</date><risdate>2006</risdate><volume>1087</volume><issue>1</issue><spage>52</spage><epage>59</epage><pages>52-59</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>Enterostatin is a pentapeptide released from its precursor protein procolipase, which is synthesized in the exocrine pancreas and gastric mucosa. As central injection of enterostatin has potent effects on feeding, we hypothesized that the procolipase may also be expressed in the brain. We confirmed the presence of preprocolipase gene expression in amygdala by reverse transcription-polymerase chain reaction and Northern blot analysis and of protein expression by Western blots. Immunohistochemical analysis using antibodies for procolipase and enterostatin identified their immunoreactivity (IR) in rat brain. Procolipase IR was present in the cytoplasm of paraventricular, amygdala, and the dorsal thalamus nucleus. Enterostatin IR was evident in the fibers of the dorsal thalamus and arcuate nucleus. In vivo injection of enterostatin antibody into rat amygdala increased food intake. 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| subjects | Amygdala Analysis of Variance Animals Antibodies - pharmacology Behavior, Animal Biochemistry and metabolism Biological and medical sciences Blotting, Northern - methods Blotting, Western - methods Brain - physiology Central nervous system Colipases - genetics Colipases - immunology Colipases - metabolism Eating - drug effects Eating - physiology Enzyme Precursors Fundamental and applied biological sciences. Psychology Gene Expression - physiology Hypothalamus Immunohistochemistry Immunohistochemistry - methods Male Northern blot Protein Precursors - genetics Protein Precursors - immunology Protein Precursors - metabolism Rats Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Messenger - metabolism RT-PCR Vertebrates: nervous system and sense organs Western blot |
| title | Procolipase gene expression in the rat brain: Source of endogenous enterostatin production in the brain |
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