A Temporal Map of Transcription Factor Activity: Mef2 Directly Regulates Target Genes at All Stages of Muscle Development
Dissecting components of key transcriptional networks is essential for understanding complex developmental processes and phenotypes. Genetic studies have highlighted the role of members of the Mef2 family of transcription factors as essential regulators in myogenesis from flies to man. To understand...
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| Veröffentlicht in: | Developmental cell 2006-06, Vol.10 (6), p.797-807 |
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| container_title | Developmental cell |
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| creator | Sandmann, Thomas Jensen, Lars J. Jakobsen, Janus S. Karzynski, Michal M. Eichenlaub, Michael P. Bork, Peer Furlong, Eileen E.M. |
| description | Dissecting components of key transcriptional networks is essential for understanding complex developmental processes and phenotypes. Genetic studies have highlighted the role of members of the Mef2 family of transcription factors as essential regulators in myogenesis from flies to man. To understand how these transcription factors control diverse processes in muscle development, we have combined chromatin immunoprecipitation analysis with gene expression profiling to obtain a temporal map of Mef2 activity during
Drosophila embryonic development. This global approach revealed three temporal patterns of Mef2 enhancer binding, providing a glimpse of dynamic enhancer use within the context of a developing embryo. Our results provide mechanistic insight into the regulation of Mef2's activity at the level of DNA binding and suggest cooperativity with the bHLH protein Twist. The number and diversity of new direct target genes indicates a much broader role for Mef2, at all stages of myogenesis, than previously anticipated. |
| doi_str_mv | 10.1016/j.devcel.2006.04.009 |
| format | Article |
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Drosophila embryonic development. This global approach revealed three temporal patterns of Mef2 enhancer binding, providing a glimpse of dynamic enhancer use within the context of a developing embryo. Our results provide mechanistic insight into the regulation of Mef2's activity at the level of DNA binding and suggest cooperativity with the bHLH protein Twist. The number and diversity of new direct target genes indicates a much broader role for Mef2, at all stages of myogenesis, than previously anticipated.</description><identifier>ISSN: 1534-5807</identifier><identifier>EISSN: 1878-1551</identifier><identifier>DOI: 10.1016/j.devcel.2006.04.009</identifier><identifier>PMID: 16740481</identifier><language>eng</language><publisher>Cambridge, MA: Elsevier Inc</publisher><subject>Animals ; Binding Sites ; Biological and medical sciences ; Cell differentiation, maturation, development, hematopoiesis ; Cell physiology ; Chromatin Immunoprecipitation ; DEVBIO ; DNA ; Drosophila ; Drosophila - embryology ; Drosophila - genetics ; Drosophila Proteins - genetics ; Drosophila Proteins - physiology ; Embryo, Nonmammalian ; Enhancer Elements, Genetic ; Fundamental and applied biological sciences. Psychology ; Gene Expression Profiling ; Gene Expression Regulation, Developmental ; Gene Targeting ; Genes, Insect ; Kinetics ; Models, Biological ; Molecular and cellular biology ; Muscle Development - physiology ; Mutation ; Myogenic Regulatory Factors - genetics ; Myogenic Regulatory Factors - physiology ; Protein Binding ; SYSBIO ; Twist-Related Protein 1 - genetics ; Twist-Related Protein 1 - physiology</subject><ispartof>Developmental cell, 2006-06, Vol.10 (6), p.797-807</ispartof><rights>2006 Elsevier Inc.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4489-8204d1421d83b96f89590e81fabfdfe77ea04ee5faa32081ae1b8cfebab950ca3</citedby><cites>FETCH-LOGICAL-c4489-8204d1421d83b96f89590e81fabfdfe77ea04ee5faa32081ae1b8cfebab950ca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1534580706001705$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17871380$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16740481$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sandmann, Thomas</creatorcontrib><creatorcontrib>Jensen, Lars J.</creatorcontrib><creatorcontrib>Jakobsen, Janus S.</creatorcontrib><creatorcontrib>Karzynski, Michal M.</creatorcontrib><creatorcontrib>Eichenlaub, Michael P.</creatorcontrib><creatorcontrib>Bork, Peer</creatorcontrib><creatorcontrib>Furlong, Eileen E.M.</creatorcontrib><title>A Temporal Map of Transcription Factor Activity: Mef2 Directly Regulates Target Genes at All Stages of Muscle Development</title><title>Developmental cell</title><addtitle>Dev Cell</addtitle><description>Dissecting components of key transcriptional networks is essential for understanding complex developmental processes and phenotypes. Genetic studies have highlighted the role of members of the Mef2 family of transcription factors as essential regulators in myogenesis from flies to man. To understand how these transcription factors control diverse processes in muscle development, we have combined chromatin immunoprecipitation analysis with gene expression profiling to obtain a temporal map of Mef2 activity during
Drosophila embryonic development. This global approach revealed three temporal patterns of Mef2 enhancer binding, providing a glimpse of dynamic enhancer use within the context of a developing embryo. Our results provide mechanistic insight into the regulation of Mef2's activity at the level of DNA binding and suggest cooperativity with the bHLH protein Twist. The number and diversity of new direct target genes indicates a much broader role for Mef2, at all stages of myogenesis, than previously anticipated.</description><subject>Animals</subject><subject>Binding Sites</subject><subject>Biological and medical sciences</subject><subject>Cell differentiation, maturation, development, hematopoiesis</subject><subject>Cell physiology</subject><subject>Chromatin Immunoprecipitation</subject><subject>DEVBIO</subject><subject>DNA</subject><subject>Drosophila</subject><subject>Drosophila - embryology</subject><subject>Drosophila - genetics</subject><subject>Drosophila Proteins - genetics</subject><subject>Drosophila Proteins - physiology</subject><subject>Embryo, Nonmammalian</subject><subject>Enhancer Elements, Genetic</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gene Targeting</subject><subject>Genes, Insect</subject><subject>Kinetics</subject><subject>Models, Biological</subject><subject>Molecular and cellular biology</subject><subject>Muscle Development - physiology</subject><subject>Mutation</subject><subject>Myogenic Regulatory Factors - genetics</subject><subject>Myogenic Regulatory Factors - physiology</subject><subject>Protein Binding</subject><subject>SYSBIO</subject><subject>Twist-Related Protein 1 - genetics</subject><subject>Twist-Related Protein 1 - physiology</subject><issn>1534-5807</issn><issn>1878-1551</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkdGK1DAUhoso7jr6BiK50bvWkzZtUy-EYdddhR0EHa_DaXoyZMi0NUkH5u3NOgN7p1fJge__OZwvy95yKDjw5uO-GOioyRUlQFOAKAC6Z9k1l63MeV3z5-lfVyKvJbRX2asQ9pBiXMLL7Io3rQAh-XV2WrMtHebJo2MbnNlk2NbjGLS3c7TTyO5Qx8mztY72aOPpE9uQKdmt9aSjO7EftFscRgpsi35Hkd3TmAaMbO0c-xlxl6ZUulmCdsRu6Uhumg80xtfZC4Mu0JvLu8p-3X3Z3nzNH77ff7tZP-RaCNnlsgQxcFHyQVZ91xjZ1R2Q5AZ7MxhqW0IQRLVBrEqQHIn3Uhvqse9q0Fitsg_n3tlPvxcKUR1sSHdzONK0BNVIqGoh2v-CvOVdJ0EkUJxB7acQPBk1e3tAf1Ic1KMbtVdnN-rRjQKhkpsUe3fpX_oDDU-hi4wEvL8AGDQ6kzxoG564Vra8Stuuss9njtLZjpa8CtrSqGn4a0UNk_33Jn8Am3-vXQ</recordid><startdate>200606</startdate><enddate>200606</enddate><creator>Sandmann, Thomas</creator><creator>Jensen, Lars J.</creator><creator>Jakobsen, Janus S.</creator><creator>Karzynski, Michal M.</creator><creator>Eichenlaub, Michael P.</creator><creator>Bork, Peer</creator><creator>Furlong, Eileen E.M.</creator><general>Elsevier Inc</general><general>Cell Press</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200606</creationdate><title>A Temporal Map of Transcription Factor Activity: Mef2 Directly Regulates Target Genes at All Stages of Muscle Development</title><author>Sandmann, Thomas ; Jensen, Lars J. ; Jakobsen, Janus S. ; Karzynski, Michal M. ; Eichenlaub, Michael P. ; Bork, Peer ; Furlong, Eileen E.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4489-8204d1421d83b96f89590e81fabfdfe77ea04ee5faa32081ae1b8cfebab950ca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Binding Sites</topic><topic>Biological and medical sciences</topic><topic>Cell differentiation, maturation, development, hematopoiesis</topic><topic>Cell physiology</topic><topic>Chromatin Immunoprecipitation</topic><topic>DEVBIO</topic><topic>DNA</topic><topic>Drosophila</topic><topic>Drosophila - embryology</topic><topic>Drosophila - genetics</topic><topic>Drosophila Proteins - genetics</topic><topic>Drosophila Proteins - physiology</topic><topic>Embryo, Nonmammalian</topic><topic>Enhancer Elements, Genetic</topic><topic>Fundamental and applied biological sciences. 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Drosophila embryonic development. This global approach revealed three temporal patterns of Mef2 enhancer binding, providing a glimpse of dynamic enhancer use within the context of a developing embryo. Our results provide mechanistic insight into the regulation of Mef2's activity at the level of DNA binding and suggest cooperativity with the bHLH protein Twist. The number and diversity of new direct target genes indicates a much broader role for Mef2, at all stages of myogenesis, than previously anticipated.</abstract><cop>Cambridge, MA</cop><pub>Elsevier Inc</pub><pmid>16740481</pmid><doi>10.1016/j.devcel.2006.04.009</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Cell Press Free Archives; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Binding Sites Biological and medical sciences Cell differentiation, maturation, development, hematopoiesis Cell physiology Chromatin Immunoprecipitation DEVBIO DNA Drosophila Drosophila - embryology Drosophila - genetics Drosophila Proteins - genetics Drosophila Proteins - physiology Embryo, Nonmammalian Enhancer Elements, Genetic Fundamental and applied biological sciences. Psychology Gene Expression Profiling Gene Expression Regulation, Developmental Gene Targeting Genes, Insect Kinetics Models, Biological Molecular and cellular biology Muscle Development - physiology Mutation Myogenic Regulatory Factors - genetics Myogenic Regulatory Factors - physiology Protein Binding SYSBIO Twist-Related Protein 1 - genetics Twist-Related Protein 1 - physiology |
| title | A Temporal Map of Transcription Factor Activity: Mef2 Directly Regulates Target Genes at All Stages of Muscle Development |
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