Comparison of the immunological memory after DNA vaccination and protein vaccination against anthrax in sheep
Currently available live spore vaccines against anthrax in animals have many drawbacks, one of which is their presumed inability to induce a long lasting immunity. In the present study we compared the immunological memory after a protein vaccination with DNA vaccinations in sheep. The antigen used w...
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| Veröffentlicht in: | Vaccine 2006-05, Vol.24 (21), p.4595-4597 |
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| creator | Hahn, Ulrike K. Aichler, Michaela Boehm, Reinhard Beyer, Wolfgang |
| description | Currently available live spore vaccines against anthrax in animals have many drawbacks, one of which is their presumed inability to induce a long lasting immunity. In the present study we compared the immunological memory after a protein vaccination with DNA vaccinations in sheep. The antigen used was the protective antigen (PA83) of
Bacillus anthracis. Sheep were vaccinated three times with either PA83 plus alhydrogel, or with one of four different plasmid DNA formulations, which all encoded either the full-length PA83 or its domain 4. Two pDNA formulations included Vaxfectin™ adjuvant, the other two were injected in PBS without adjuvant. Initially, the antibody titres of protein vaccinated sheep were significantly higher than the titres of pDNA vaccinated sheep. After 5 months, however, the antibody titres of protein vaccinated sheep had dropped remarkably, while the titres of all four pDNA vaccinated groups were either stable or had increased. Humoral responses of sheep immunised with pDNA formulated with Vaxfectin™ adjuvant were higher than the responses of the corresponding groups that received pDNA in PBS only. |
| doi_str_mv | 10.1016/j.vaccine.2005.08.025 |
| format | Article |
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Bacillus anthracis. Sheep were vaccinated three times with either PA83 plus alhydrogel, or with one of four different plasmid DNA formulations, which all encoded either the full-length PA83 or its domain 4. Two pDNA formulations included Vaxfectin™ adjuvant, the other two were injected in PBS without adjuvant. Initially, the antibody titres of protein vaccinated sheep were significantly higher than the titres of pDNA vaccinated sheep. After 5 months, however, the antibody titres of protein vaccinated sheep had dropped remarkably, while the titres of all four pDNA vaccinated groups were either stable or had increased. Humoral responses of sheep immunised with pDNA formulated with Vaxfectin™ adjuvant were higher than the responses of the corresponding groups that received pDNA in PBS only.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2005.08.025</identifier><identifier>PMID: 16198452</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Animals ; Antigens ; Bacillus anthracis ; Bacterial Proteins - administration & dosage ; Base Sequence ; Deoxyribonucleic acid ; DNA ; DNA Primers ; DNA vaccine ; Enzyme-Linked Immunosorbent Assay ; Immunization ; Immunologic Memory ; Lymphocytes ; Plasmids ; Proteins ; Sheep ; Sheep Diseases - prevention & control ; Vaccines ; Vaccines, DNA - administration & dosage ; Vaxfectin</subject><ispartof>Vaccine, 2006-05, Vol.24 (21), p.4595-4597</ispartof><rights>2005 Elsevier Ltd</rights><rights>Copyright Elsevier Limited May 22, 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-9b0114140192a4c896bc9b9c5b18c8fb0f723eb11fc17da870d0fdccbc1f5a5a3</citedby><cites>FETCH-LOGICAL-c422t-9b0114140192a4c896bc9b9c5b18c8fb0f723eb11fc17da870d0fdccbc1f5a5a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1559006556?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995,64385,64387,64389,72469</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16198452$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hahn, Ulrike K.</creatorcontrib><creatorcontrib>Aichler, Michaela</creatorcontrib><creatorcontrib>Boehm, Reinhard</creatorcontrib><creatorcontrib>Beyer, Wolfgang</creatorcontrib><title>Comparison of the immunological memory after DNA vaccination and protein vaccination against anthrax in sheep</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Currently available live spore vaccines against anthrax in animals have many drawbacks, one of which is their presumed inability to induce a long lasting immunity. In the present study we compared the immunological memory after a protein vaccination with DNA vaccinations in sheep. The antigen used was the protective antigen (PA83) of
Bacillus anthracis. Sheep were vaccinated three times with either PA83 plus alhydrogel, or with one of four different plasmid DNA formulations, which all encoded either the full-length PA83 or its domain 4. Two pDNA formulations included Vaxfectin™ adjuvant, the other two were injected in PBS without adjuvant. Initially, the antibody titres of protein vaccinated sheep were significantly higher than the titres of pDNA vaccinated sheep. After 5 months, however, the antibody titres of protein vaccinated sheep had dropped remarkably, while the titres of all four pDNA vaccinated groups were either stable or had increased. Humoral responses of sheep immunised with pDNA formulated with Vaxfectin™ adjuvant were higher than the responses of the corresponding groups that received pDNA in PBS only.</description><subject>Animals</subject><subject>Antigens</subject><subject>Bacillus anthracis</subject><subject>Bacterial Proteins - administration & dosage</subject><subject>Base Sequence</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Primers</subject><subject>DNA vaccine</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Immunization</subject><subject>Immunologic Memory</subject><subject>Lymphocytes</subject><subject>Plasmids</subject><subject>Proteins</subject><subject>Sheep</subject><subject>Sheep Diseases - prevention & control</subject><subject>Vaccines</subject><subject>Vaccines, DNA - administration & dosage</subject><subject>Vaxfectin</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkcFu1DAQhi0EokvhEUCRkLglzDix45xQtbSAVLUXkLhZjjPuepXEi51U9O1xtSshuPRkyfP9M2N_jL1FqBBQftxX98ZaP1PFAUQFqgIunrENqrYuuUD1nG2Ay6ZsEH6esVcp7SGDNXYv2RlK7FQj-IZN2zAdTPQpzEVwxbKjwk_TOocx3HlrxmKiKcSHwriFYvH55qI4jjWLzwkzD8UhhoX8_O_9nfFzWnJ92UXzu8jltCM6vGYvnBkTvTmd5-zH1eX37dfy-vbLt-3FdWkbzpey6wGxwQaw46axqpO97frOih6VVa4H1_KaekRnsR2MamEAN1jbW3TCCFOfsw_Hvnm5XyulRU8-WRpHM1NYk5YK6qaT7ZMgtpxziU0G3_8H7sMa5_wIjUJ0AFIImSlxpGwMKUVy-hD9ZOKDRtCP2vRen7TpR20alM7acu7dqfvaTzT8TZ08ZeDTEaD8a_eeok7W02xp8JHsoofgnxjxBzbfrMQ</recordid><startdate>20060522</startdate><enddate>20060522</enddate><creator>Hahn, Ulrike K.</creator><creator>Aichler, Michaela</creator><creator>Boehm, Reinhard</creator><creator>Beyer, Wolfgang</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20060522</creationdate><title>Comparison of the immunological memory after DNA vaccination and protein vaccination against anthrax in sheep</title><author>Hahn, Ulrike K. ; Aichler, Michaela ; Boehm, Reinhard ; Beyer, Wolfgang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-9b0114140192a4c896bc9b9c5b18c8fb0f723eb11fc17da870d0fdccbc1f5a5a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Antigens</topic><topic>Bacillus anthracis</topic><topic>Bacterial Proteins - 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Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hahn, Ulrike K.</au><au>Aichler, Michaela</au><au>Boehm, Reinhard</au><au>Beyer, Wolfgang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of the immunological memory after DNA vaccination and protein vaccination against anthrax in sheep</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2006-05-22</date><risdate>2006</risdate><volume>24</volume><issue>21</issue><spage>4595</spage><epage>4597</epage><pages>4595-4597</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><abstract>Currently available live spore vaccines against anthrax in animals have many drawbacks, one of which is their presumed inability to induce a long lasting immunity. In the present study we compared the immunological memory after a protein vaccination with DNA vaccinations in sheep. The antigen used was the protective antigen (PA83) of
Bacillus anthracis. Sheep were vaccinated three times with either PA83 plus alhydrogel, or with one of four different plasmid DNA formulations, which all encoded either the full-length PA83 or its domain 4. Two pDNA formulations included Vaxfectin™ adjuvant, the other two were injected in PBS without adjuvant. Initially, the antibody titres of protein vaccinated sheep were significantly higher than the titres of pDNA vaccinated sheep. After 5 months, however, the antibody titres of protein vaccinated sheep had dropped remarkably, while the titres of all four pDNA vaccinated groups were either stable or had increased. Humoral responses of sheep immunised with pDNA formulated with Vaxfectin™ adjuvant were higher than the responses of the corresponding groups that received pDNA in PBS only.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>16198452</pmid><doi>10.1016/j.vaccine.2005.08.025</doi><tpages>3</tpages></addata></record> |
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| subjects | Animals Antigens Bacillus anthracis Bacterial Proteins - administration & dosage Base Sequence Deoxyribonucleic acid DNA DNA Primers DNA vaccine Enzyme-Linked Immunosorbent Assay Immunization Immunologic Memory Lymphocytes Plasmids Proteins Sheep Sheep Diseases - prevention & control Vaccines Vaccines, DNA - administration & dosage Vaxfectin |
| title | Comparison of the immunological memory after DNA vaccination and protein vaccination against anthrax in sheep |
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