Development of detection methods for ruminant interleukin (IL)-4
Recombinant bovine IL-4 (rbo IL-4) was transiently expressed in COS-7 cells. Mice were immunised with a plasmid encoding rbo IL-4 and boosted with rbo IL-4. A number of monoclonal antibodies (mAb) were generated that reacted with rbo IL-4 in an ELISA and these cloned hybridomas were termed CC311, CC...
Gespeichert in:
| Veröffentlicht in: | Journal of immunological methods 2005-06, Vol.301 (1), p.114-123 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 123 |
|---|---|
| container_issue | 1 |
| container_start_page | 114 |
| container_title | Journal of immunological methods |
| container_volume | 301 |
| creator | Hope, J.C. Kwong, L.S. Thom, M. Sopp, P. Mwangi, W. Brown, W.C. Palmer, G.H. Wattegedera, S. Entrican, G. Howard, C.J. |
| description | Recombinant bovine IL-4 (rbo IL-4) was transiently expressed in COS-7 cells. Mice were immunised with a plasmid encoding rbo IL-4 and boosted with rbo IL-4. A number of monoclonal antibodies (mAb) were generated that reacted with rbo IL-4 in an ELISA and these cloned hybridomas were termed CC311, CC312, CC313 and CC314. A pair of mAb (CC313 and CC314) was identified that together could be used to detect both recombinant and native bovine IL-4 by ELISA and a luminometric detection method was applied to the ELISA. Using this method native bovine IL-4 was detected in supernatants of PBMC stimulated with mitogens. In addition, high level secretion of IL-4 by
Fasciola hepatica specific Th2 clones, but not by a
Babesia bovis specific Th1 clone, was confirmed. The ELISA was also able to detect recombinant ovine IL-4. The pair of mAb used for ELISA could also be used for the detection of IL-4 spot forming cells by ELISPOT. In addition intracytoplasmic expression of IL-4 could be detected. The ability to detect ruminant IL-4 by three methods: ELISA, ELISPOT and by flow cytometric analysis of intracytoplasmic expression will permit studies of the role of this important cytokine in the immunology and pathogenesis of animal diseases. |
| doi_str_mv | 10.1016/j.jim.2005.04.010 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68034218</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0022175905001171</els_id><sourcerecordid>68034218</sourcerecordid><originalsourceid>FETCH-LOGICAL-c382t-36b65be306bb307c449fd86ecddc11dc4629024b1df499fd0731c9ae7c61ca213</originalsourceid><addsrcrecordid>eNqF0LFP3DAUx3GrKioH9A_oUmWqYEh4z3acRCytDmhPOomFzlZiv6i-JvHVTpD47zG6k7rB5MHf9xs-jH1BKBBQXe-KnRsLDlAWIAtA-MBWWFc8rxooP7IVAOc5VmVzys5i3AGkRMEndoplUzVKqBX7fktPNPj9SNOc-T6zNJOZnZ-ykeY_3sas9yELy-imNhVumikMtPx1U3a52V7l8oKd9O0Q6fPxPWe_7-8e17_y7cPPzfrHNjei5nMuVKfKjgSorhNQGSmb3taKjLUG0RqpeANcdmh72aQvqASapqXKKDQtR3HOvh1298H_WyjOenTR0DC0E_klalWDkBzrd8PkoVAApBAPoQk-xkC93gc3tuFZI-hXX73TyVe_-mqQOuGlm6_H8aUbyf6_OIKm4OYQULJ4chR0NI4mQ9aFBKutd2_MvwB_64ob</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17561300</pqid></control><display><type>article</type><title>Development of detection methods for ruminant interleukin (IL)-4</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Hope, J.C. ; Kwong, L.S. ; Thom, M. ; Sopp, P. ; Mwangi, W. ; Brown, W.C. ; Palmer, G.H. ; Wattegedera, S. ; Entrican, G. ; Howard, C.J.</creator><creatorcontrib>Hope, J.C. ; Kwong, L.S. ; Thom, M. ; Sopp, P. ; Mwangi, W. ; Brown, W.C. ; Palmer, G.H. ; Wattegedera, S. ; Entrican, G. ; Howard, C.J.</creatorcontrib><description>Recombinant bovine IL-4 (rbo IL-4) was transiently expressed in COS-7 cells. Mice were immunised with a plasmid encoding rbo IL-4 and boosted with rbo IL-4. A number of monoclonal antibodies (mAb) were generated that reacted with rbo IL-4 in an ELISA and these cloned hybridomas were termed CC311, CC312, CC313 and CC314. A pair of mAb (CC313 and CC314) was identified that together could be used to detect both recombinant and native bovine IL-4 by ELISA and a luminometric detection method was applied to the ELISA. Using this method native bovine IL-4 was detected in supernatants of PBMC stimulated with mitogens. In addition, high level secretion of IL-4 by
Fasciola hepatica specific Th2 clones, but not by a
Babesia bovis specific Th1 clone, was confirmed. The ELISA was also able to detect recombinant ovine IL-4. The pair of mAb used for ELISA could also be used for the detection of IL-4 spot forming cells by ELISPOT. In addition intracytoplasmic expression of IL-4 could be detected. The ability to detect ruminant IL-4 by three methods: ELISA, ELISPOT and by flow cytometric analysis of intracytoplasmic expression will permit studies of the role of this important cytokine in the immunology and pathogenesis of animal diseases.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/j.jim.2005.04.010</identifier><identifier>PMID: 15979636</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Antibodies, Monoclonal - immunology ; Antigens - immunology ; Babesia bovis ; Cattle - immunology ; Cercopithecus aethiops ; COS Cells ; ELISA ; ELISPOT ; Enzyme-Linked Immunosorbent Assay ; Fasciola hepatica ; Interleukin-4 ; Interleukin-4 - analysis ; Interleukin-4 - immunology ; Th1 Cells - secretion ; Th2 Cells - secretion</subject><ispartof>Journal of immunological methods, 2005-06, Vol.301 (1), p.114-123</ispartof><rights>2005 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-36b65be306bb307c449fd86ecddc11dc4629024b1df499fd0731c9ae7c61ca213</citedby><cites>FETCH-LOGICAL-c382t-36b65be306bb307c449fd86ecddc11dc4629024b1df499fd0731c9ae7c61ca213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0022175905001171$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15979636$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hope, J.C.</creatorcontrib><creatorcontrib>Kwong, L.S.</creatorcontrib><creatorcontrib>Thom, M.</creatorcontrib><creatorcontrib>Sopp, P.</creatorcontrib><creatorcontrib>Mwangi, W.</creatorcontrib><creatorcontrib>Brown, W.C.</creatorcontrib><creatorcontrib>Palmer, G.H.</creatorcontrib><creatorcontrib>Wattegedera, S.</creatorcontrib><creatorcontrib>Entrican, G.</creatorcontrib><creatorcontrib>Howard, C.J.</creatorcontrib><title>Development of detection methods for ruminant interleukin (IL)-4</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>Recombinant bovine IL-4 (rbo IL-4) was transiently expressed in COS-7 cells. Mice were immunised with a plasmid encoding rbo IL-4 and boosted with rbo IL-4. A number of monoclonal antibodies (mAb) were generated that reacted with rbo IL-4 in an ELISA and these cloned hybridomas were termed CC311, CC312, CC313 and CC314. A pair of mAb (CC313 and CC314) was identified that together could be used to detect both recombinant and native bovine IL-4 by ELISA and a luminometric detection method was applied to the ELISA. Using this method native bovine IL-4 was detected in supernatants of PBMC stimulated with mitogens. In addition, high level secretion of IL-4 by
Fasciola hepatica specific Th2 clones, but not by a
Babesia bovis specific Th1 clone, was confirmed. The ELISA was also able to detect recombinant ovine IL-4. The pair of mAb used for ELISA could also be used for the detection of IL-4 spot forming cells by ELISPOT. In addition intracytoplasmic expression of IL-4 could be detected. The ability to detect ruminant IL-4 by three methods: ELISA, ELISPOT and by flow cytometric analysis of intracytoplasmic expression will permit studies of the role of this important cytokine in the immunology and pathogenesis of animal diseases.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antigens - immunology</subject><subject>Babesia bovis</subject><subject>Cattle - immunology</subject><subject>Cercopithecus aethiops</subject><subject>COS Cells</subject><subject>ELISA</subject><subject>ELISPOT</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fasciola hepatica</subject><subject>Interleukin-4</subject><subject>Interleukin-4 - analysis</subject><subject>Interleukin-4 - immunology</subject><subject>Th1 Cells - secretion</subject><subject>Th2 Cells - secretion</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0LFP3DAUx3GrKioH9A_oUmWqYEh4z3acRCytDmhPOomFzlZiv6i-JvHVTpD47zG6k7rB5MHf9xs-jH1BKBBQXe-KnRsLDlAWIAtA-MBWWFc8rxooP7IVAOc5VmVzys5i3AGkRMEndoplUzVKqBX7fktPNPj9SNOc-T6zNJOZnZ-ykeY_3sas9yELy-imNhVumikMtPx1U3a52V7l8oKd9O0Q6fPxPWe_7-8e17_y7cPPzfrHNjei5nMuVKfKjgSorhNQGSmb3taKjLUG0RqpeANcdmh72aQvqASapqXKKDQtR3HOvh1298H_WyjOenTR0DC0E_klalWDkBzrd8PkoVAApBAPoQk-xkC93gc3tuFZI-hXX73TyVe_-mqQOuGlm6_H8aUbyf6_OIKm4OYQULJ4chR0NI4mQ9aFBKutd2_MvwB_64ob</recordid><startdate>20050601</startdate><enddate>20050601</enddate><creator>Hope, J.C.</creator><creator>Kwong, L.S.</creator><creator>Thom, M.</creator><creator>Sopp, P.</creator><creator>Mwangi, W.</creator><creator>Brown, W.C.</creator><creator>Palmer, G.H.</creator><creator>Wattegedera, S.</creator><creator>Entrican, G.</creator><creator>Howard, C.J.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20050601</creationdate><title>Development of detection methods for ruminant interleukin (IL)-4</title><author>Hope, J.C. ; Kwong, L.S. ; Thom, M. ; Sopp, P. ; Mwangi, W. ; Brown, W.C. ; Palmer, G.H. ; Wattegedera, S. ; Entrican, G. ; Howard, C.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-36b65be306bb307c449fd86ecddc11dc4629024b1df499fd0731c9ae7c61ca213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antigens - immunology</topic><topic>Babesia bovis</topic><topic>Cattle - immunology</topic><topic>Cercopithecus aethiops</topic><topic>COS Cells</topic><topic>ELISA</topic><topic>ELISPOT</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fasciola hepatica</topic><topic>Interleukin-4</topic><topic>Interleukin-4 - analysis</topic><topic>Interleukin-4 - immunology</topic><topic>Th1 Cells - secretion</topic><topic>Th2 Cells - secretion</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hope, J.C.</creatorcontrib><creatorcontrib>Kwong, L.S.</creatorcontrib><creatorcontrib>Thom, M.</creatorcontrib><creatorcontrib>Sopp, P.</creatorcontrib><creatorcontrib>Mwangi, W.</creatorcontrib><creatorcontrib>Brown, W.C.</creatorcontrib><creatorcontrib>Palmer, G.H.</creatorcontrib><creatorcontrib>Wattegedera, S.</creatorcontrib><creatorcontrib>Entrican, G.</creatorcontrib><creatorcontrib>Howard, C.J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hope, J.C.</au><au>Kwong, L.S.</au><au>Thom, M.</au><au>Sopp, P.</au><au>Mwangi, W.</au><au>Brown, W.C.</au><au>Palmer, G.H.</au><au>Wattegedera, S.</au><au>Entrican, G.</au><au>Howard, C.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of detection methods for ruminant interleukin (IL)-4</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>2005-06-01</date><risdate>2005</risdate><volume>301</volume><issue>1</issue><spage>114</spage><epage>123</epage><pages>114-123</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><abstract>Recombinant bovine IL-4 (rbo IL-4) was transiently expressed in COS-7 cells. Mice were immunised with a plasmid encoding rbo IL-4 and boosted with rbo IL-4. A number of monoclonal antibodies (mAb) were generated that reacted with rbo IL-4 in an ELISA and these cloned hybridomas were termed CC311, CC312, CC313 and CC314. A pair of mAb (CC313 and CC314) was identified that together could be used to detect both recombinant and native bovine IL-4 by ELISA and a luminometric detection method was applied to the ELISA. Using this method native bovine IL-4 was detected in supernatants of PBMC stimulated with mitogens. In addition, high level secretion of IL-4 by
Fasciola hepatica specific Th2 clones, but not by a
Babesia bovis specific Th1 clone, was confirmed. The ELISA was also able to detect recombinant ovine IL-4. The pair of mAb used for ELISA could also be used for the detection of IL-4 spot forming cells by ELISPOT. In addition intracytoplasmic expression of IL-4 could be detected. The ability to detect ruminant IL-4 by three methods: ELISA, ELISPOT and by flow cytometric analysis of intracytoplasmic expression will permit studies of the role of this important cytokine in the immunology and pathogenesis of animal diseases.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>15979636</pmid><doi>10.1016/j.jim.2005.04.010</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1759 |
| ispartof | Journal of immunological methods, 2005-06, Vol.301 (1), p.114-123 |
| issn | 0022-1759 1872-7905 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68034218 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Antibodies, Monoclonal - immunology Antigens - immunology Babesia bovis Cattle - immunology Cercopithecus aethiops COS Cells ELISA ELISPOT Enzyme-Linked Immunosorbent Assay Fasciola hepatica Interleukin-4 Interleukin-4 - analysis Interleukin-4 - immunology Th1 Cells - secretion Th2 Cells - secretion |
| title | Development of detection methods for ruminant interleukin (IL)-4 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T20%3A10%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20detection%20methods%20for%20ruminant%20interleukin%20(IL)-4&rft.jtitle=Journal%20of%20immunological%20methods&rft.au=Hope,%20J.C.&rft.date=2005-06-01&rft.volume=301&rft.issue=1&rft.spage=114&rft.epage=123&rft.pages=114-123&rft.issn=0022-1759&rft.eissn=1872-7905&rft_id=info:doi/10.1016/j.jim.2005.04.010&rft_dat=%3Cproquest_cross%3E68034218%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17561300&rft_id=info:pmid/15979636&rft_els_id=S0022175905001171&rfr_iscdi=true |