Long-term RNA interference from optimized siRNA expression constructs in adult mice

DNA constructs for small interfering RNA (siRNA) expression in mammalian cells have the potential for longer-term target gene knockdown than synthetic siRNAs. We compared in adult mice the efficacy and longevity of target gene knockdown from siRNA expression cassettes contained in plasmids, PCR-gene...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biochemical and biophysical research communications 2005-08, Vol.334 (1), p.117-127
Hauptverfasser:	Wooddell, Christine I., Van Hout, Cristopher V., Reppen, Thomas, Lewis, David L., Herweijer, Hans
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals CHO Cells Cricetinae Cricetulus Gene Silencing Gene Targeting - methods Genes, Reporter - genetics H1 promoter HeLa Cells Humans In vivo gene transfer Longevity of expression Mice Mice, Inbred C57BL Mice, Knockout Non-viral gene transfer PCR product Polymerase Chain Reaction - methods RNA Interference RNA polymerase III promoter RNA, Small Interfering - administration & dosage RNA, Small Interfering - genetics siRNA expression vectors Time Factors Transfection - methods U6 promoter
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!