Psychrophilic trypsin-type protease from Serratia proteamaculans

A preparative method for purification of a novel protease from the psychrotolerant Gram-negative microorganism Serratia proteamaculans (PSP) was developed using affinity chromatography on BPTI-Sepharose. It yielded electrophoretically homogeneous PSP preparation of 60 kD. The PSP properties (tempera...

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Veröffentlicht in:	Biochemistry (Moscow) 2006-05, Vol.71 (5), p.563-570
Hauptverfasser:	Mikhailova, A G, Likhareva, V V, Khairullin, R F, Lubenets, N L, Rumsh, L D, Demidyuk, I V, Kostrov, S V
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity chromatography Bacteria Bacterial Proteins - antagonists & inhibitors Bacterial Proteins - isolation & purification Bacterial Proteins - metabolism Chromatography, Affinity - methods Chromatography, Ion Exchange - methods Enzyme Stability - drug effects Enzymes Hydrogen-Ion Concentration Kinetics Nocardia corallina Peptide Hydrolases - isolation & purification Peptide Hydrolases - metabolism Protease Protease Inhibitors - pharmacology Proteinase Psychrotrophic organisms Serratia Serratia - enzymology Serratia proteamaculans Substrate inhibition Substrate Specificity Temperature Trypsin Trypsin - metabolism
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