Identification, isolation, and partial characterization of a novel Streptococcus uberis adhesion molecule (SUAM)
The ability to attach to the host cell surface has been considered an important virulence strategy in many bovine mammary gland pathogens, including Streptococcus uberis. Research conducted in our laboratory lead to the identification of an S. uberis adhesion molecule (SUAM) with affinity for bovine...
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| Veröffentlicht in: | Veterinary microbiology 2006-06, Vol.115 (1), p.183-191 |
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| creator | Almeida, Raul A. Luther, Douglas A. Park, Hee-Myung Oliver, Stephen P. |
| description | The ability to attach to the host cell surface has been considered an important virulence strategy in many bovine mammary gland pathogens, including
Streptococcus uberis. Research conducted in our laboratory lead to the identification of an
S. uberis adhesion molecule (SUAM) with affinity for bovine lactoferrin (LF) and delineation of its role in adherence of
S. uberis to bovine mammary epithelial cells. Using a selected bacterial surface protein extraction protocol and affinity chromatography, a 112-kDa protein that had a similar molecular mass and the LF affinity as one of the identified
S. uberis LBP described by Fang and Oliver in 1999 was found. To further characterize SUAM, the N-terminal amino acid sequence of this protein was elucidated. A protein query versus translated database TBLASTN search of the National Center for Biotechnology (NCBI), non-redundant database, nr, with the LBP N-terminal amino acid sequence showed no significant similarity with previous entries. Antibodies directed against SUAM and a 17 amino acid long N-terminal sequence (pep-SUAM) inhibited adherence to and internalization of
S. uberis UT888 into bovine mammary epithelial cells. Data presented suggests that we have discovered a novel bacterial protein involved in the pathogenesis of this economically important mastitis pathogen. |
| doi_str_mv | 10.1016/j.vetmic.2006.02.005 |
| format | Article |
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Streptococcus uberis. Research conducted in our laboratory lead to the identification of an
S. uberis adhesion molecule (SUAM) with affinity for bovine lactoferrin (LF) and delineation of its role in adherence of
S. uberis to bovine mammary epithelial cells. Using a selected bacterial surface protein extraction protocol and affinity chromatography, a 112-kDa protein that had a similar molecular mass and the LF affinity as one of the identified
S. uberis LBP described by Fang and Oliver in 1999 was found. To further characterize SUAM, the N-terminal amino acid sequence of this protein was elucidated. A protein query versus translated database TBLASTN search of the National Center for Biotechnology (NCBI), non-redundant database, nr, with the LBP N-terminal amino acid sequence showed no significant similarity with previous entries. Antibodies directed against SUAM and a 17 amino acid long N-terminal sequence (pep-SUAM) inhibited adherence to and internalization of
S. uberis UT888 into bovine mammary epithelial cells. Data presented suggests that we have discovered a novel bacterial protein involved in the pathogenesis of this economically important mastitis pathogen.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/j.vetmic.2006.02.005</identifier><identifier>PMID: 16564651</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Adhesins, Bacterial - chemistry ; Adhesins, Bacterial - genetics ; Adhesins, Bacterial - isolation & purification ; Adhesins, Bacterial - metabolism ; Adhesion molecule ; Amino Acid Sequence ; animal pathogenic bacteria ; Animals ; antibody detection ; bacterial adhesion ; Bacterial Adhesion - genetics ; Bacterial Adhesion - physiology ; Bacterial Proteins - chemistry ; Bacterial Proteins - genetics ; Bacterial Proteins - isolation & purification ; Bacterial Proteins - metabolism ; Bacteriology ; binding proteins ; Biological and medical sciences ; Bovine mastitis ; Carrier Proteins - chemistry ; Carrier Proteins - genetics ; Carrier Proteins - isolation & purification ; Carrier Proteins - metabolism ; Cattle ; Cell Line ; Chromatography, Affinity - veterinary ; Electrophoresis, Polyacrylamide Gel - veterinary ; Epithelial Cells ; Female ; Fundamental and applied biological sciences. Psychology ; isolation ; Lactoferrin - metabolism ; Mammary Glands, Animal - microbiology ; Microbiology ; Miscellaneous ; Molecular Sequence Data ; Molecular Weight ; pathogen identification ; Pathogenesis ; strain differences ; strains ; Streptococcus - classification ; Streptococcus - isolation & purification ; Streptococcus - metabolism ; Streptococcus - pathogenicity ; Streptococcus uberis ; Virulence Factors</subject><ispartof>Veterinary microbiology, 2006-06, Vol.115 (1), p.183-191</ispartof><rights>2006 Elsevier B.V.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c511t-6ebeffcf098babc42ab1db62e98244bbbecf8c09b147c4876f4fb188029ad7f13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378113506000496$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17850783$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16564651$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Almeida, Raul A.</creatorcontrib><creatorcontrib>Luther, Douglas A.</creatorcontrib><creatorcontrib>Park, Hee-Myung</creatorcontrib><creatorcontrib>Oliver, Stephen P.</creatorcontrib><title>Identification, isolation, and partial characterization of a novel Streptococcus uberis adhesion molecule (SUAM)</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>The ability to attach to the host cell surface has been considered an important virulence strategy in many bovine mammary gland pathogens, including
Streptococcus uberis. Research conducted in our laboratory lead to the identification of an
S. uberis adhesion molecule (SUAM) with affinity for bovine lactoferrin (LF) and delineation of its role in adherence of
S. uberis to bovine mammary epithelial cells. Using a selected bacterial surface protein extraction protocol and affinity chromatography, a 112-kDa protein that had a similar molecular mass and the LF affinity as one of the identified
S. uberis LBP described by Fang and Oliver in 1999 was found. To further characterize SUAM, the N-terminal amino acid sequence of this protein was elucidated. A protein query versus translated database TBLASTN search of the National Center for Biotechnology (NCBI), non-redundant database, nr, with the LBP N-terminal amino acid sequence showed no significant similarity with previous entries. Antibodies directed against SUAM and a 17 amino acid long N-terminal sequence (pep-SUAM) inhibited adherence to and internalization of
S. uberis UT888 into bovine mammary epithelial cells. Data presented suggests that we have discovered a novel bacterial protein involved in the pathogenesis of this economically important mastitis pathogen.</description><subject>Adhesins, Bacterial - chemistry</subject><subject>Adhesins, Bacterial - genetics</subject><subject>Adhesins, Bacterial - isolation & purification</subject><subject>Adhesins, Bacterial - metabolism</subject><subject>Adhesion molecule</subject><subject>Amino Acid Sequence</subject><subject>animal pathogenic bacteria</subject><subject>Animals</subject><subject>antibody detection</subject><subject>bacterial adhesion</subject><subject>Bacterial Adhesion - genetics</subject><subject>Bacterial Adhesion - physiology</subject><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - isolation & purification</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacteriology</subject><subject>binding proteins</subject><subject>Biological and medical sciences</subject><subject>Bovine mastitis</subject><subject>Carrier Proteins - chemistry</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - isolation & purification</subject><subject>Carrier Proteins - metabolism</subject><subject>Cattle</subject><subject>Cell Line</subject><subject>Chromatography, Affinity - veterinary</subject><subject>Electrophoresis, Polyacrylamide Gel - veterinary</subject><subject>Epithelial Cells</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>isolation</subject><subject>Lactoferrin - metabolism</subject><subject>Mammary Glands, Animal - microbiology</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Molecular Sequence Data</subject><subject>Molecular Weight</subject><subject>pathogen identification</subject><subject>Pathogenesis</subject><subject>strain differences</subject><subject>strains</subject><subject>Streptococcus - classification</subject><subject>Streptococcus - isolation & purification</subject><subject>Streptococcus - metabolism</subject><subject>Streptococcus - pathogenicity</subject><subject>Streptococcus uberis</subject><subject>Virulence Factors</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0d-L1DAQB_Aiiree_geifVEUbJ1J2zR9EY7DHwcnPqz7HJLpxMvSbdakXdC_3q67cG_6lIF8Zhjmm2XPEUoElO-35YGnnadSAMgSRAnQPMhWqNqqEE0tHmYrqFpVIFbNRfYkpS0A1J2Ex9kFykbWssFVtr_peZy882QmH8Z3uU9hOJdm7PO9iZM3Q053JhqaOPrff3_z4HKTj-HAQ76eIu-nQIFoTvlsF5Ry099xOsJdGJjmgfM3683V17dPs0fODImfnd_LbPPp4_frL8Xtt88311e3BTWIUyHZsnPkoFPWWKqFsdhbKbhToq6ttUxOEXQW65Zq1UpXO4tKgehM3zqsLrPXp7n7GH7OnCa984l4GMzIYU5aLlQ0ovovxBYVVFIusD5BiiGlyE7vo9-Z-Esj6GMkeqtPkehjJBqEXiJZ2l6c5892x_190zmDBbw6A5PIDC6akXy6d61qoFXHRV-enDNBmx_LkfVmLQArQESFIBbx4SR4OezBc9SJPI_EvY9Mk-6D__eufwAuULbn</recordid><startdate>20060615</startdate><enddate>20060615</enddate><creator>Almeida, Raul A.</creator><creator>Luther, Douglas A.</creator><creator>Park, Hee-Myung</creator><creator>Oliver, Stephen P.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20060615</creationdate><title>Identification, isolation, and partial characterization of a novel Streptococcus uberis adhesion molecule (SUAM)</title><author>Almeida, Raul A. ; Luther, Douglas A. ; Park, Hee-Myung ; Oliver, Stephen P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c511t-6ebeffcf098babc42ab1db62e98244bbbecf8c09b147c4876f4fb188029ad7f13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adhesins, Bacterial - chemistry</topic><topic>Adhesins, Bacterial - genetics</topic><topic>Adhesins, Bacterial - isolation & purification</topic><topic>Adhesins, Bacterial - metabolism</topic><topic>Adhesion molecule</topic><topic>Amino Acid Sequence</topic><topic>animal pathogenic bacteria</topic><topic>Animals</topic><topic>antibody detection</topic><topic>bacterial adhesion</topic><topic>Bacterial Adhesion - genetics</topic><topic>Bacterial Adhesion - physiology</topic><topic>Bacterial Proteins - chemistry</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - isolation & purification</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacteriology</topic><topic>binding proteins</topic><topic>Biological and medical sciences</topic><topic>Bovine mastitis</topic><topic>Carrier Proteins - chemistry</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - isolation & purification</topic><topic>Carrier Proteins - metabolism</topic><topic>Cattle</topic><topic>Cell Line</topic><topic>Chromatography, Affinity - veterinary</topic><topic>Electrophoresis, Polyacrylamide Gel - veterinary</topic><topic>Epithelial Cells</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>isolation</topic><topic>Lactoferrin - metabolism</topic><topic>Mammary Glands, Animal - microbiology</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Molecular Sequence Data</topic><topic>Molecular Weight</topic><topic>pathogen identification</topic><topic>Pathogenesis</topic><topic>strain differences</topic><topic>strains</topic><topic>Streptococcus - classification</topic><topic>Streptococcus - isolation & purification</topic><topic>Streptococcus - metabolism</topic><topic>Streptococcus - pathogenicity</topic><topic>Streptococcus uberis</topic><topic>Virulence Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Almeida, Raul A.</creatorcontrib><creatorcontrib>Luther, Douglas A.</creatorcontrib><creatorcontrib>Park, Hee-Myung</creatorcontrib><creatorcontrib>Oliver, Stephen P.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Almeida, Raul A.</au><au>Luther, Douglas A.</au><au>Park, Hee-Myung</au><au>Oliver, Stephen P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification, isolation, and partial characterization of a novel Streptococcus uberis adhesion molecule (SUAM)</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2006-06-15</date><risdate>2006</risdate><volume>115</volume><issue>1</issue><spage>183</spage><epage>191</epage><pages>183-191</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>The ability to attach to the host cell surface has been considered an important virulence strategy in many bovine mammary gland pathogens, including
Streptococcus uberis. Research conducted in our laboratory lead to the identification of an
S. uberis adhesion molecule (SUAM) with affinity for bovine lactoferrin (LF) and delineation of its role in adherence of
S. uberis to bovine mammary epithelial cells. Using a selected bacterial surface protein extraction protocol and affinity chromatography, a 112-kDa protein that had a similar molecular mass and the LF affinity as one of the identified
S. uberis LBP described by Fang and Oliver in 1999 was found. To further characterize SUAM, the N-terminal amino acid sequence of this protein was elucidated. A protein query versus translated database TBLASTN search of the National Center for Biotechnology (NCBI), non-redundant database, nr, with the LBP N-terminal amino acid sequence showed no significant similarity with previous entries. Antibodies directed against SUAM and a 17 amino acid long N-terminal sequence (pep-SUAM) inhibited adherence to and internalization of
S. uberis UT888 into bovine mammary epithelial cells. Data presented suggests that we have discovered a novel bacterial protein involved in the pathogenesis of this economically important mastitis pathogen.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>16564651</pmid><doi>10.1016/j.vetmic.2006.02.005</doi><tpages>9</tpages></addata></record> |
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| ispartof | Veterinary microbiology, 2006-06, Vol.115 (1), p.183-191 |
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| subjects | Adhesins, Bacterial - chemistry Adhesins, Bacterial - genetics Adhesins, Bacterial - isolation & purification Adhesins, Bacterial - metabolism Adhesion molecule Amino Acid Sequence animal pathogenic bacteria Animals antibody detection bacterial adhesion Bacterial Adhesion - genetics Bacterial Adhesion - physiology Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - isolation & purification Bacterial Proteins - metabolism Bacteriology binding proteins Biological and medical sciences Bovine mastitis Carrier Proteins - chemistry Carrier Proteins - genetics Carrier Proteins - isolation & purification Carrier Proteins - metabolism Cattle Cell Line Chromatography, Affinity - veterinary Electrophoresis, Polyacrylamide Gel - veterinary Epithelial Cells Female Fundamental and applied biological sciences. Psychology isolation Lactoferrin - metabolism Mammary Glands, Animal - microbiology Microbiology Miscellaneous Molecular Sequence Data Molecular Weight pathogen identification Pathogenesis strain differences strains Streptococcus - classification Streptococcus - isolation & purification Streptococcus - metabolism Streptococcus - pathogenicity Streptococcus uberis Virulence Factors |
| title | Identification, isolation, and partial characterization of a novel Streptococcus uberis adhesion molecule (SUAM) |
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