First isolation and molecular characterization of Toxoplasma gondii from finishing pigs from São Paulo State, Brazil
Toxoplasma gondii infection is widely prevalent in humans in Brazil. Among the food animals, pigs are considered the most important meat source of T. gondii for infection in humans. In the present study, we report the first isolation of viable T. gondii from finishing pigs in Brazil. Antibodies to T...
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| Veröffentlicht in: | Veterinary parasitology 2005-08, Vol.131 (3), p.207-211 |
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| creator | de A. dos Santos, Cláudia B. Carvalho, Ângela C.F.B. de Ragozo, Alessandra M.A. Soares, Rodrigo M. Amaku, Marcos Yai, Lúcia E.O. Dubey, J.P. Gennari, Solange M. |
| description | Toxoplasma gondii infection is widely prevalent in humans in Brazil. Among the food animals, pigs are considered the most important meat source of
T. gondii for infection in humans. In the present study, we report the first isolation of viable
T. gondii from finishing pigs in Brazil. Antibodies to
T. gondii were found in 49 (17%) of 286 pigs prior slaughter using the modified agglutination test (MAT) at a serum dilution of 1:25. Attempts were made to isolate
T. gondii from 28 seropositive pigs. Samples of heart, brain, and tongue from each pig were pooled, digested in acid pepsin, and bioassayed in five mice per pig. Viable
T. gondii was isolated from seven pigs; all isolates were lethal for mice. Restriction fragment length polymorphism on products of SAG2 locus amplified by PCR revealed that two isolates were Type I and five were Type III. The results indicate that phenotypically and genetically
T. gondii isolates from pigs from Brazil are distinct from isolates of
T. gondii from pigs in the USA. |
| doi_str_mv | 10.1016/j.vetpar.2005.04.039 |
| format | Article |
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T. gondii for infection in humans. In the present study, we report the first isolation of viable
T. gondii from finishing pigs in Brazil. Antibodies to
T. gondii were found in 49 (17%) of 286 pigs prior slaughter using the modified agglutination test (MAT) at a serum dilution of 1:25. Attempts were made to isolate
T. gondii from 28 seropositive pigs. Samples of heart, brain, and tongue from each pig were pooled, digested in acid pepsin, and bioassayed in five mice per pig. Viable
T. gondii was isolated from seven pigs; all isolates were lethal for mice. Restriction fragment length polymorphism on products of SAG2 locus amplified by PCR revealed that two isolates were Type I and five were Type III. The results indicate that phenotypically and genetically
T. gondii isolates from pigs from Brazil are distinct from isolates of
T. gondii from pigs in the USA.</description><identifier>ISSN: 0304-4017</identifier><identifier>EISSN: 1873-2550</identifier><identifier>DOI: 10.1016/j.vetpar.2005.04.039</identifier><identifier>PMID: 15951111</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Agglutination Tests - veterinary ; Animals ; Antibodies, Protozoan - blood ; antibody detection ; Antigens, Protozoan - chemistry ; Antigens, Protozoan - genetics ; Bioassay ; bioassays ; Biological Assay - veterinary ; Brazil ; DNA, Protozoan - chemistry ; DNA, Protozoan - genetics ; Female ; Genotype ; Genotyping ; isolation ; Mice ; Pigs ; Polymerase Chain Reaction - veterinary ; Protozoan Proteins - chemistry ; Protozoan Proteins - genetics ; restriction fragment length polymorphism ; seroprevalence ; strain differences ; Swine ; Swine Diseases - parasitology ; Toxoplasma - genetics ; Toxoplasma - isolation & purification ; Toxoplasma gondii ; toxoplasmosis ; Toxoplasmosis, Animal - parasitology ; Zoonoses - parasitology</subject><ispartof>Veterinary parasitology, 2005-08, Vol.131 (3), p.207-211</ispartof><rights>2005 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-fbaa4ad9f8ee1b3bf55b1c222c884129900934e87a9b230bc1cd9f5655d97aac3</citedby><cites>FETCH-LOGICAL-c415t-fbaa4ad9f8ee1b3bf55b1c222c884129900934e87a9b230bc1cd9f5655d97aac3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0304401705002165$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15951111$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de A. dos Santos, Cláudia B.</creatorcontrib><creatorcontrib>Carvalho, Ângela C.F.B. de</creatorcontrib><creatorcontrib>Ragozo, Alessandra M.A.</creatorcontrib><creatorcontrib>Soares, Rodrigo M.</creatorcontrib><creatorcontrib>Amaku, Marcos</creatorcontrib><creatorcontrib>Yai, Lúcia E.O.</creatorcontrib><creatorcontrib>Dubey, J.P.</creatorcontrib><creatorcontrib>Gennari, Solange M.</creatorcontrib><title>First isolation and molecular characterization of Toxoplasma gondii from finishing pigs from São Paulo State, Brazil</title><title>Veterinary parasitology</title><addtitle>Vet Parasitol</addtitle><description>Toxoplasma gondii infection is widely prevalent in humans in Brazil. Among the food animals, pigs are considered the most important meat source of
T. gondii for infection in humans. In the present study, we report the first isolation of viable
T. gondii from finishing pigs in Brazil. Antibodies to
T. gondii were found in 49 (17%) of 286 pigs prior slaughter using the modified agglutination test (MAT) at a serum dilution of 1:25. Attempts were made to isolate
T. gondii from 28 seropositive pigs. Samples of heart, brain, and tongue from each pig were pooled, digested in acid pepsin, and bioassayed in five mice per pig. Viable
T. gondii was isolated from seven pigs; all isolates were lethal for mice. Restriction fragment length polymorphism on products of SAG2 locus amplified by PCR revealed that two isolates were Type I and five were Type III. The results indicate that phenotypically and genetically
T. gondii isolates from pigs from Brazil are distinct from isolates of
T. gondii from pigs in the USA.</description><subject>Agglutination Tests - veterinary</subject><subject>Animals</subject><subject>Antibodies, Protozoan - blood</subject><subject>antibody detection</subject><subject>Antigens, Protozoan - chemistry</subject><subject>Antigens, Protozoan - genetics</subject><subject>Bioassay</subject><subject>bioassays</subject><subject>Biological Assay - veterinary</subject><subject>Brazil</subject><subject>DNA, Protozoan - chemistry</subject><subject>DNA, Protozoan - genetics</subject><subject>Female</subject><subject>Genotype</subject><subject>Genotyping</subject><subject>isolation</subject><subject>Mice</subject><subject>Pigs</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>Protozoan Proteins - chemistry</subject><subject>Protozoan Proteins - genetics</subject><subject>restriction fragment length polymorphism</subject><subject>seroprevalence</subject><subject>strain differences</subject><subject>Swine</subject><subject>Swine Diseases - parasitology</subject><subject>Toxoplasma - genetics</subject><subject>Toxoplasma - isolation & purification</subject><subject>Toxoplasma gondii</subject><subject>toxoplasmosis</subject><subject>Toxoplasmosis, Animal - parasitology</subject><subject>Zoonoses - parasitology</subject><issn>0304-4017</issn><issn>1873-2550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi0EokvhDRD4xKkJ49hO4gtSqVpAqlSkbc_WxHG2XiVxsJMK-jo8Ci-Gq6zUW-vLSPY3_1jzEfKeQc6AlZ_3-Z2dJwx5ASBzEDlw9YJsWF3xrJASXpINcBCZAFYdkTcx7gFAQFm9JkdMKsnS2ZDlwoU4Uxd9j7PzI8WxpYPvrVl6DNTcYkAz2-Du12ff0Wv_2089xgHpzo-tc7QLfqCdG128deOOTm4X17vtv7-e_sSl93Q742xP6NeA965_S1512Ef77lCPyc3F-fXZ9-zy6tuPs9PLzAgm56xrEAW2qqutZQ1vOikbZoqiMHUtWKEUgOLC1hWqpuDQGGYSLEspW1UhGn5MPq25U_C_FhtnPbhobN_jaP0SdVkDq5UqnwVZVXCZRiRQrKAJPsZgOz0FN2D4oxnoBy96r1cv-sGLBqGTl9T24ZC_NINtH5sOIhLwcQU69Bp3wUV9sy2AcUihtRAiEV9WwqaF3TkbdDTOjsa2Llgz69a7p__wH9uIrF0</recordid><startdate>20050810</startdate><enddate>20050810</enddate><creator>de A. dos Santos, Cláudia B.</creator><creator>Carvalho, Ângela C.F.B. de</creator><creator>Ragozo, Alessandra M.A.</creator><creator>Soares, Rodrigo M.</creator><creator>Amaku, Marcos</creator><creator>Yai, Lúcia E.O.</creator><creator>Dubey, J.P.</creator><creator>Gennari, Solange M.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20050810</creationdate><title>First isolation and molecular characterization of Toxoplasma gondii from finishing pigs from São Paulo State, Brazil</title><author>de A. dos Santos, Cláudia B. ; Carvalho, Ângela C.F.B. de ; Ragozo, Alessandra M.A. ; Soares, Rodrigo M. ; Amaku, Marcos ; Yai, Lúcia E.O. ; Dubey, J.P. ; Gennari, Solange M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-fbaa4ad9f8ee1b3bf55b1c222c884129900934e87a9b230bc1cd9f5655d97aac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Agglutination Tests - veterinary</topic><topic>Animals</topic><topic>Antibodies, Protozoan - blood</topic><topic>antibody detection</topic><topic>Antigens, Protozoan - chemistry</topic><topic>Antigens, Protozoan - genetics</topic><topic>Bioassay</topic><topic>bioassays</topic><topic>Biological Assay - veterinary</topic><topic>Brazil</topic><topic>DNA, Protozoan - chemistry</topic><topic>DNA, Protozoan - genetics</topic><topic>Female</topic><topic>Genotype</topic><topic>Genotyping</topic><topic>isolation</topic><topic>Mice</topic><topic>Pigs</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>Protozoan Proteins - chemistry</topic><topic>Protozoan Proteins - genetics</topic><topic>restriction fragment length polymorphism</topic><topic>seroprevalence</topic><topic>strain differences</topic><topic>Swine</topic><topic>Swine Diseases - parasitology</topic><topic>Toxoplasma - genetics</topic><topic>Toxoplasma - isolation & purification</topic><topic>Toxoplasma gondii</topic><topic>toxoplasmosis</topic><topic>Toxoplasmosis, Animal - parasitology</topic><topic>Zoonoses - parasitology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de A. dos Santos, Cláudia B.</creatorcontrib><creatorcontrib>Carvalho, Ângela C.F.B. de</creatorcontrib><creatorcontrib>Ragozo, Alessandra M.A.</creatorcontrib><creatorcontrib>Soares, Rodrigo M.</creatorcontrib><creatorcontrib>Amaku, Marcos</creatorcontrib><creatorcontrib>Yai, Lúcia E.O.</creatorcontrib><creatorcontrib>Dubey, J.P.</creatorcontrib><creatorcontrib>Gennari, Solange M.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de A. dos Santos, Cláudia B.</au><au>Carvalho, Ângela C.F.B. de</au><au>Ragozo, Alessandra M.A.</au><au>Soares, Rodrigo M.</au><au>Amaku, Marcos</au><au>Yai, Lúcia E.O.</au><au>Dubey, J.P.</au><au>Gennari, Solange M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>First isolation and molecular characterization of Toxoplasma gondii from finishing pigs from São Paulo State, Brazil</atitle><jtitle>Veterinary parasitology</jtitle><addtitle>Vet Parasitol</addtitle><date>2005-08-10</date><risdate>2005</risdate><volume>131</volume><issue>3</issue><spage>207</spage><epage>211</epage><pages>207-211</pages><issn>0304-4017</issn><eissn>1873-2550</eissn><abstract>Toxoplasma gondii infection is widely prevalent in humans in Brazil. Among the food animals, pigs are considered the most important meat source of
T. gondii for infection in humans. In the present study, we report the first isolation of viable
T. gondii from finishing pigs in Brazil. Antibodies to
T. gondii were found in 49 (17%) of 286 pigs prior slaughter using the modified agglutination test (MAT) at a serum dilution of 1:25. Attempts were made to isolate
T. gondii from 28 seropositive pigs. Samples of heart, brain, and tongue from each pig were pooled, digested in acid pepsin, and bioassayed in five mice per pig. Viable
T. gondii was isolated from seven pigs; all isolates were lethal for mice. Restriction fragment length polymorphism on products of SAG2 locus amplified by PCR revealed that two isolates were Type I and five were Type III. The results indicate that phenotypically and genetically
T. gondii isolates from pigs from Brazil are distinct from isolates of
T. gondii from pigs in the USA.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>15951111</pmid><doi>10.1016/j.vetpar.2005.04.039</doi><tpages>5</tpages></addata></record> |
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| ispartof | Veterinary parasitology, 2005-08, Vol.131 (3), p.207-211 |
| issn | 0304-4017 1873-2550 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Agglutination Tests - veterinary Animals Antibodies, Protozoan - blood antibody detection Antigens, Protozoan - chemistry Antigens, Protozoan - genetics Bioassay bioassays Biological Assay - veterinary Brazil DNA, Protozoan - chemistry DNA, Protozoan - genetics Female Genotype Genotyping isolation Mice Pigs Polymerase Chain Reaction - veterinary Protozoan Proteins - chemistry Protozoan Proteins - genetics restriction fragment length polymorphism seroprevalence strain differences Swine Swine Diseases - parasitology Toxoplasma - genetics Toxoplasma - isolation & purification Toxoplasma gondii toxoplasmosis Toxoplasmosis, Animal - parasitology Zoonoses - parasitology |
| title | First isolation and molecular characterization of Toxoplasma gondii from finishing pigs from São Paulo State, Brazil |
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