Precise method for the measurement of catalase activity in honey

An improved method is reported for the determination of catalase activity in honey. We tested different dialysis membranes, dialysis fluid compositions and amounts, dialysis temperatures, sample amounts, and dialysis times. The best results were obtained by dialysis of 7.50 g sample in a cellulose d...

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Veröffentlicht in:	Journal of AOAC International 2005-05, Vol.88 (3), p.800-804
Hauptverfasser:	Huidobro, J.F, Sanchez, M.P, Muniategui, S, Sancho, M.T
Format:	Artikel
Sprache:	eng
Schlagworte:	analytical methods Biological and medical sciences botanical composition Buffers Calibration catalase Catalase - analysis Catalase - metabolism Catalysis Cellulose Chemistry Techniques, Analytical - methods Confectionery products and chocolate industries, honey Dialysis Dianisidine - analysis enzyme activity food analysis food composition Food industries Fundamental and applied biological sciences. Psychology Honey hydrogen peroxide Hydrogen Peroxide - chemistry Hydrogen-Ion Concentration Kinetics Measurement Membranes - metabolism Methods Peroxidase - analysis Phosphates Phosphates - chemistry Polysaccharides Reproducibility of Results Research Design Spectrophotometry Temperature Time Factors
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