Yatein from Chamaecyparis obtusa suppresses herpes simplex virus type 1 replication in HeLa cells by interruption the immediate-early gene expression
Inhibitory effects of methanolic extracts from nine Chinese herbs on herpes simplex virus type 1 (HSV-1) replication were studied. By a bioassay-guided fractionation procedure, yatein (C 22H 23O 7; M.W.399) was isolated from Chamaecyparis obtusa; yatein significantly suppressed HSV-1 multiplication...
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| Veröffentlicht in: | Antiviral research 2006-07, Vol.70 (3), p.112-120 |
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| creator | Kuo, Yuh-Chi Kuo, Yueh-Hsiung Lin, Yuang-Lian Tsai, Wei-Jern |
| description | Inhibitory effects of methanolic extracts from nine Chinese herbs on herpes simplex virus type 1 (HSV-1) replication were studied. By a bioassay-guided fractionation procedure, yatein (C
22H
23O
7; M.W.399) was isolated from
Chamaecyparis obtusa; yatein significantly suppressed HSV-1 multiplication in HeLa cells without apparent cytotoxicity. To further localize the point in the HSV-1 replication cycle where arrest occurred, a set of key regulatory events leading to the viral multiplication was examined, including viral immediate-early (α) and late (γ) gene expression and DNA replication. Results indicated that levels of glycoprotein B (gB) and gC mRNA expression in HeLa cells were impeded by yatein. Data from polymerase chain reaction showed that replication of HSV-1 DNA in HeLa cells was arrested by yatein. Furthermore, yatein decreased ICP0 and ICP4 gene expression in HeLa cells. Results of an electrophoretic mobility shift assay demonstrated that yatein interrupted the formation of α-
trans-induction factor/C1/Oct-1/GARAT multiprotein complex. The mechanisms of antiviral action of yatein seem to be mediated, by inhibiting HSV-1 α gene expression, including expression of the ICP0 and ICP4 genes, and by arresting HSV-1 DNA synthesis and structural protein expression in HeLa cells. These results suggest that yatein is an antiviral agent against HSV-1 replication. |
| doi_str_mv | 10.1016/j.antiviral.2006.01.011 |
| format | Article |
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22H
23O
7; M.W.399) was isolated from
Chamaecyparis obtusa; yatein significantly suppressed HSV-1 multiplication in HeLa cells without apparent cytotoxicity. To further localize the point in the HSV-1 replication cycle where arrest occurred, a set of key regulatory events leading to the viral multiplication was examined, including viral immediate-early (α) and late (γ) gene expression and DNA replication. Results indicated that levels of glycoprotein B (gB) and gC mRNA expression in HeLa cells were impeded by yatein. Data from polymerase chain reaction showed that replication of HSV-1 DNA in HeLa cells was arrested by yatein. Furthermore, yatein decreased ICP0 and ICP4 gene expression in HeLa cells. Results of an electrophoretic mobility shift assay demonstrated that yatein interrupted the formation of α-
trans-induction factor/C1/Oct-1/GARAT multiprotein complex. The mechanisms of antiviral action of yatein seem to be mediated, by inhibiting HSV-1 α gene expression, including expression of the ICP0 and ICP4 genes, and by arresting HSV-1 DNA synthesis and structural protein expression in HeLa cells. These results suggest that yatein is an antiviral agent against HSV-1 replication.</description><identifier>ISSN: 0166-3542</identifier><identifier>EISSN: 1872-9096</identifier><identifier>DOI: 10.1016/j.antiviral.2006.01.011</identifier><identifier>PMID: 16540181</identifier><identifier>CODEN: ARSRDR</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>4-Butyrolactone - analogs & derivatives ; 4-Butyrolactone - pharmacology ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Antiviral agents ; Antiviral Agents - pharmacology ; Biological and medical sciences ; Chamaecyparis - chemistry ; Chamaecyparis obtusa ; Dioxoles - pharmacology ; Drugs, Chinese Herbal ; General pharmacology ; Genes, Immediate-Early ; HeLa Cells ; Herpes simplex virus 1 ; Herpesvirus 1, Human - drug effects ; Herpesvirus 1, Human - physiology ; HSV-1 ; Humans ; ICP4 ; Immediate-Early Proteins - drug effects ; Immediate-Early Proteins - genetics ; Immediate-Early Proteins - metabolism ; Medical sciences ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; Virus Replication - drug effects ; Yatein ; α- Trans-induction factor</subject><ispartof>Antiviral research, 2006-07, Vol.70 (3), p.112-120</ispartof><rights>2006 Elsevier B.V.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c496t-28cda93b818dce58d9564e6502d9385001804ea1bd91edf18d3a41f3279f4ad43</citedby><cites>FETCH-LOGICAL-c496t-28cda93b818dce58d9564e6502d9385001804ea1bd91edf18d3a41f3279f4ad43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.antiviral.2006.01.011$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17825245$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16540181$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kuo, Yuh-Chi</creatorcontrib><creatorcontrib>Kuo, Yueh-Hsiung</creatorcontrib><creatorcontrib>Lin, Yuang-Lian</creatorcontrib><creatorcontrib>Tsai, Wei-Jern</creatorcontrib><title>Yatein from Chamaecyparis obtusa suppresses herpes simplex virus type 1 replication in HeLa cells by interruption the immediate-early gene expression</title><title>Antiviral research</title><addtitle>Antiviral Res</addtitle><description>Inhibitory effects of methanolic extracts from nine Chinese herbs on herpes simplex virus type 1 (HSV-1) replication were studied. By a bioassay-guided fractionation procedure, yatein (C
22H
23O
7; M.W.399) was isolated from
Chamaecyparis obtusa; yatein significantly suppressed HSV-1 multiplication in HeLa cells without apparent cytotoxicity. To further localize the point in the HSV-1 replication cycle where arrest occurred, a set of key regulatory events leading to the viral multiplication was examined, including viral immediate-early (α) and late (γ) gene expression and DNA replication. Results indicated that levels of glycoprotein B (gB) and gC mRNA expression in HeLa cells were impeded by yatein. Data from polymerase chain reaction showed that replication of HSV-1 DNA in HeLa cells was arrested by yatein. Furthermore, yatein decreased ICP0 and ICP4 gene expression in HeLa cells. Results of an electrophoretic mobility shift assay demonstrated that yatein interrupted the formation of α-
trans-induction factor/C1/Oct-1/GARAT multiprotein complex. The mechanisms of antiviral action of yatein seem to be mediated, by inhibiting HSV-1 α gene expression, including expression of the ICP0 and ICP4 genes, and by arresting HSV-1 DNA synthesis and structural protein expression in HeLa cells. These results suggest that yatein is an antiviral agent against HSV-1 replication.</description><subject>4-Butyrolactone - analogs & derivatives</subject><subject>4-Butyrolactone - pharmacology</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Antiviral agents</subject><subject>Antiviral Agents - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Chamaecyparis - chemistry</subject><subject>Chamaecyparis obtusa</subject><subject>Dioxoles - pharmacology</subject><subject>Drugs, Chinese Herbal</subject><subject>General pharmacology</subject><subject>Genes, Immediate-Early</subject><subject>HeLa Cells</subject><subject>Herpes simplex virus 1</subject><subject>Herpesvirus 1, Human - drug effects</subject><subject>Herpesvirus 1, Human - physiology</subject><subject>HSV-1</subject><subject>Humans</subject><subject>ICP4</subject><subject>Immediate-Early Proteins - drug effects</subject><subject>Immediate-Early Proteins - genetics</subject><subject>Immediate-Early Proteins - metabolism</subject><subject>Medical sciences</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>Virus Replication - drug effects</subject><subject>Yatein</subject><subject>α- Trans-induction factor</subject><issn>0166-3542</issn><issn>1872-9096</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9u1DAQxiMEokvhFcAXuGXxJE5iH6tVoUgrcYEDJ8txJqxX-WM8TtU8CO-Lt7uix0ojjeT5zXzj-bLsA_AtcKg_H7dmiu7eBTNsC87rLYcU8CLbgGyKXHFVv8w2iazzshLFVfaG6MgT2Cj5OruCuhIcJGyyv79MRDexPswj2x3MaNCu3gRHbG7jQobR4n1AIiR2wOBTIjf6AR9Ykl-IxdUjAxbQD86a6OaJpXl3uDfM4jAQa9f0EDGExT9W4wGZG0fsXJLO0YRhZb9xQoYPj0KJeZu96s1A-O6Sr7OfX25_7O7y_fev33Y3-9wKVce8kLYzqmwlyM5iJTtV1QLrihedKmXF0xe5QANtpwC7PlGlEdCXRaN6YTpRXmefznN9mP8sSFGPjk5bmwnnhXQtOQgF5bMgNAUo1ZzA5gzaMBMF7LUPbjRh1cD1yTp91P-t0yfrNIcUkDrfXySWNh3nqe_iVQI-XgBD1gx9MJN19MQ1sqgKUSXu5sxhuty9w6DJOpxsOnhAG3U3u2eX-QeVGr89</recordid><startdate>20060701</startdate><enddate>20060701</enddate><creator>Kuo, Yuh-Chi</creator><creator>Kuo, Yueh-Hsiung</creator><creator>Lin, Yuang-Lian</creator><creator>Tsai, Wei-Jern</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20060701</creationdate><title>Yatein from Chamaecyparis obtusa suppresses herpes simplex virus type 1 replication in HeLa cells by interruption the immediate-early gene expression</title><author>Kuo, Yuh-Chi ; Kuo, Yueh-Hsiung ; Lin, Yuang-Lian ; Tsai, Wei-Jern</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c496t-28cda93b818dce58d9564e6502d9385001804ea1bd91edf18d3a41f3279f4ad43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>4-Butyrolactone - analogs & derivatives</topic><topic>4-Butyrolactone - pharmacology</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Antiviral agents</topic><topic>Antiviral Agents - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Chamaecyparis - chemistry</topic><topic>Chamaecyparis obtusa</topic><topic>Dioxoles - pharmacology</topic><topic>Drugs, Chinese Herbal</topic><topic>General pharmacology</topic><topic>Genes, Immediate-Early</topic><topic>HeLa Cells</topic><topic>Herpes simplex virus 1</topic><topic>Herpesvirus 1, Human - drug effects</topic><topic>Herpesvirus 1, Human - physiology</topic><topic>HSV-1</topic><topic>Humans</topic><topic>ICP4</topic><topic>Immediate-Early Proteins - drug effects</topic><topic>Immediate-Early Proteins - genetics</topic><topic>Immediate-Early Proteins - metabolism</topic><topic>Medical sciences</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>Virus Replication - drug effects</topic><topic>Yatein</topic><topic>α- Trans-induction factor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kuo, Yuh-Chi</creatorcontrib><creatorcontrib>Kuo, Yueh-Hsiung</creatorcontrib><creatorcontrib>Lin, Yuang-Lian</creatorcontrib><creatorcontrib>Tsai, Wei-Jern</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Antiviral research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kuo, Yuh-Chi</au><au>Kuo, Yueh-Hsiung</au><au>Lin, Yuang-Lian</au><au>Tsai, Wei-Jern</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Yatein from Chamaecyparis obtusa suppresses herpes simplex virus type 1 replication in HeLa cells by interruption the immediate-early gene expression</atitle><jtitle>Antiviral research</jtitle><addtitle>Antiviral Res</addtitle><date>2006-07-01</date><risdate>2006</risdate><volume>70</volume><issue>3</issue><spage>112</spage><epage>120</epage><pages>112-120</pages><issn>0166-3542</issn><eissn>1872-9096</eissn><coden>ARSRDR</coden><abstract>Inhibitory effects of methanolic extracts from nine Chinese herbs on herpes simplex virus type 1 (HSV-1) replication were studied. By a bioassay-guided fractionation procedure, yatein (C
22H
23O
7; M.W.399) was isolated from
Chamaecyparis obtusa; yatein significantly suppressed HSV-1 multiplication in HeLa cells without apparent cytotoxicity. To further localize the point in the HSV-1 replication cycle where arrest occurred, a set of key regulatory events leading to the viral multiplication was examined, including viral immediate-early (α) and late (γ) gene expression and DNA replication. Results indicated that levels of glycoprotein B (gB) and gC mRNA expression in HeLa cells were impeded by yatein. Data from polymerase chain reaction showed that replication of HSV-1 DNA in HeLa cells was arrested by yatein. Furthermore, yatein decreased ICP0 and ICP4 gene expression in HeLa cells. Results of an electrophoretic mobility shift assay demonstrated that yatein interrupted the formation of α-
trans-induction factor/C1/Oct-1/GARAT multiprotein complex. The mechanisms of antiviral action of yatein seem to be mediated, by inhibiting HSV-1 α gene expression, including expression of the ICP0 and ICP4 genes, and by arresting HSV-1 DNA synthesis and structural protein expression in HeLa cells. These results suggest that yatein is an antiviral agent against HSV-1 replication.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>16540181</pmid><doi>10.1016/j.antiviral.2006.01.011</doi><tpages>9</tpages></addata></record> |
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| ispartof | Antiviral research, 2006-07, Vol.70 (3), p.112-120 |
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| subjects | 4-Butyrolactone - analogs & derivatives 4-Butyrolactone - pharmacology Antibiotics. Antiinfectious agents. Antiparasitic agents Antiviral agents Antiviral Agents - pharmacology Biological and medical sciences Chamaecyparis - chemistry Chamaecyparis obtusa Dioxoles - pharmacology Drugs, Chinese Herbal General pharmacology Genes, Immediate-Early HeLa Cells Herpes simplex virus 1 Herpesvirus 1, Human - drug effects Herpesvirus 1, Human - physiology HSV-1 Humans ICP4 Immediate-Early Proteins - drug effects Immediate-Early Proteins - genetics Immediate-Early Proteins - metabolism Medical sciences Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Virus Replication - drug effects Yatein α- Trans-induction factor |
| title | Yatein from Chamaecyparis obtusa suppresses herpes simplex virus type 1 replication in HeLa cells by interruption the immediate-early gene expression |
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