Detection of Mycobacterium tuberculosis complex with Real Time PCR: Comparison of different primer-probe sets based on the IS6110 element
The sensitivity and specificity to detect Mycobacterium tuberculosis complex of four Real Time PCR primer-probe sets was compared. Three sets targeted nearly the same location on the IS6110 sequence and set 4 targeted a location 200 bp downstream on IS6110. Real Time PCR's with sets 1, 2 and 3...
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| Veröffentlicht in: | Journal of microbiological methods 2006-07, Vol.66 (1), p.177-180 |
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| container_issue | 1 |
| container_start_page | 177 |
| container_title | Journal of microbiological methods |
| container_volume | 66 |
| creator | Savelkoul, Paul H.M. Catsburg, Arnold Mulder, Sije Oostendorp, Ludo Schirm, Jurjen Wilke, Hans van der Zanden, Adri G.M. Noordhoek, Gerda T. |
| description | The sensitivity and specificity to detect
Mycobacterium tuberculosis complex of four Real Time PCR primer-probe sets was compared. Three sets targeted nearly the same location on the
IS6110 sequence and set 4 targeted a location 200 bp downstream on
IS6110. Real Time PCR's with sets 1, 2 and 3 were carried out with co-amplification of a modified target as an internal amplification control. By testing identical DNA samples it was shown that small changes in primer and probe sequences result in differences in the performance of the assays, regarding analytical sensitivity and specificity. |
| doi_str_mv | 10.1016/j.mimet.2005.12.003 |
| format | Article |
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IS6110 sequence and set 4 targeted a location 200 bp downstream on
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Mycobacterium tuberculosis complex of four Real Time PCR primer-probe sets was compared. Three sets targeted nearly the same location on the
IS6110 sequence and set 4 targeted a location 200 bp downstream on
IS6110. Real Time PCR's with sets 1, 2 and 3 were carried out with co-amplification of a modified target as an internal amplification control. By testing identical DNA samples it was shown that small changes in primer and probe sequences result in differences in the performance of the assays, regarding analytical sensitivity and specificity.</description><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>DNA Primers - genetics</subject><subject>DNA Probes - genetics</subject><subject>DNA Transposable Elements - genetics</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics</subject><subject>IS6110</subject><subject>Microbiology</subject><subject>Mycobacterium tuberculosis</subject><subject>Mycobacterium tuberculosis - genetics</subject><subject>Mycobacterium tuberculosis - isolation & purification</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Real Time PCR</subject><subject>Sensitivity and Specificity</subject><subject>Tuberculosis - microbiology</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcuOFCEUhonROO3oE5gYNrqrkksVUCYuTHubZIxmHNcEqEOGTlXRAqXOI_jW0nYns9MVC77_5xw-hJ5S0lJCxctdO4cZSssI6VvKWkL4PbShSrJG8X64jzaVko0klJ2hRznvCKE979RDdEZFx6SkbIN-v4UCroS44Ojxp1sXrXEFUlhnXFYLya1TzCFjF-f9BL_wz1Bu8BWYCV_X1_GX7dUrvK13JoV8LBmD95BgKXifKpKafYoWcIaSsTUZRly5cgP44quglGCYYK70Y_TAmynDk9N5jr69f3e9_dhcfv5wsX1z2biOqtIMgslRqK6rUc8dk16RzjjbWQUD2NH6XsLonfWC9-MgvTA9dT1XwnDuOsLP0Ytjbx3r-wq56DlkB9NkFohr1kIRygcx_BekklGuiKwgP4IuxZwTeH1Y3KRbTYk-qNI7_VeVPqjSlOmqqqaenepXO8N4lzm5qcDzE2CyM5NPZnEh33FS9YPih4VeHzmov_YjQNLZBVgcjCFVtXqM4Z-D_AF96rP_</recordid><startdate>20060701</startdate><enddate>20060701</enddate><creator>Savelkoul, Paul H.M.</creator><creator>Catsburg, Arnold</creator><creator>Mulder, Sije</creator><creator>Oostendorp, Ludo</creator><creator>Schirm, Jurjen</creator><creator>Wilke, Hans</creator><creator>van der Zanden, Adri G.M.</creator><creator>Noordhoek, Gerda T.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20060701</creationdate><title>Detection of Mycobacterium tuberculosis complex with Real Time PCR: Comparison of different primer-probe sets based on the IS6110 element</title><author>Savelkoul, Paul H.M. ; Catsburg, Arnold ; Mulder, Sije ; Oostendorp, Ludo ; Schirm, Jurjen ; Wilke, Hans ; van der Zanden, Adri G.M. ; Noordhoek, Gerda T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-9627d6844110f3c27f804acb4b8e9ebdbf57edfcbf635d97f6a51c5386a33c403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>DNA Primers - genetics</topic><topic>DNA Probes - genetics</topic><topic>DNA Transposable Elements - genetics</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>Fundamental and applied biological sciences. 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IS6110 sequence and set 4 targeted a location 200 bp downstream on
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| ispartof | Journal of microbiological methods, 2006-07, Vol.66 (1), p.177-180 |
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| subjects | Bacteriology Biological and medical sciences DNA Primers - genetics DNA Probes - genetics DNA Transposable Elements - genetics DNA, Bacterial - chemistry DNA, Bacterial - genetics Fundamental and applied biological sciences. Psychology Genetics IS6110 Microbiology Mycobacterium tuberculosis Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - isolation & purification Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Polymerase Chain Reaction - methods Real Time PCR Sensitivity and Specificity Tuberculosis - microbiology |
| title | Detection of Mycobacterium tuberculosis complex with Real Time PCR: Comparison of different primer-probe sets based on the IS6110 element |
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