Deletion of smooth muscle α-actin alters blood-retina barrier permeability and retinal function
Vascular smooth muscle (SM) cells and pericytes are essential for normal vascular development. SM alpha-actin null mice were used to determine whether vascular SM and pericyte contractile functions, and not merely their presence, are necessary for vascular development, normal blood-retina barrier (B...
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| Veröffentlicht in: | Investigative ophthalmology & visual science 2006-06, Vol.47 (6), p.2693-2700 |
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| container_title | Investigative ophthalmology & visual science |
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| creator | TOMASEK, James J HAAKSMA, Carol J SCHWARTZ, Robert J VUONG, Duc T ZHANG, Sarah X ASH, John D MA, Jian-Xing AL-UBAIDI, Muayyad R |
| description | Vascular smooth muscle (SM) cells and pericytes are essential for normal vascular development. SM alpha-actin null mice were used to determine whether vascular SM and pericyte contractile functions, and not merely their presence, are necessary for vascular development, normal blood-retina barrier (BRB) permeability, and retinal function.
Age-matched SM alpha-actin null and wild-type mice were analyzed. Retinal structure, vascular pattern, and SM cell and pericyte distribution were analyzed histologically. Retinal vascular permeability (RVP) was measured with the Evans blue dye method. Electroretinography (ERG) was performed to evaluate retinal function.
Deletion of SM alpha-actin did not result in any alterations in retinal morphology, vascular pattern, or SM cell and pericyte ensheathing of vessels in SM alpha-actin null mice. A significant increase in RVP was observed in SM alpha-actin null mice at both postnatal day (P)50 and P75 (P |
| doi_str_mv | 10.1167/iovs.05-1297 |
| format | Article |
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Age-matched SM alpha-actin null and wild-type mice were analyzed. Retinal structure, vascular pattern, and SM cell and pericyte distribution were analyzed histologically. Retinal vascular permeability (RVP) was measured with the Evans blue dye method. Electroretinography (ERG) was performed to evaluate retinal function.
Deletion of SM alpha-actin did not result in any alterations in retinal morphology, vascular pattern, or SM cell and pericyte ensheathing of vessels in SM alpha-actin null mice. A significant increase in RVP was observed in SM alpha-actin null mice at both postnatal day (P)50 and P75 (P<0.05 and P<0.001, respectively). ERG analysis demonstrated a significant reduction in both rod and cone function in SM alpha-actin null mice at P22, P45, and P75 (P<0.01 at all ages).
These results demonstrate that SM alpha-actin in SM cells and pericytes is not necessary for the formation of a normal retinal vascular pattern; however, SM alpha-actin is necessary for SM cells and pericytes to interact with endothelial cells to form a fully functional BRB. These results are important in understanding the role of contractile gene expression in the maintenance and function of the BRB and may provide a model for studying pathologic conditions, such as diabetes, that alter the function of this barrier.</description><identifier>ISSN: 0146-0404</identifier><identifier>ISSN: 1552-5783</identifier><identifier>EISSN: 1552-5783</identifier><identifier>DOI: 10.1167/iovs.05-1297</identifier><identifier>PMID: 16723488</identifier><identifier>CODEN: IOVSDA</identifier><language>eng</language><publisher>Rockville, MD: Association for Research in Vision and Ophtalmology</publisher><subject>Actins - physiology ; Albumins - metabolism ; Animals ; Biological and medical sciences ; Blood-Retinal Barrier - physiology ; Blotting, Western ; Capillary Permeability - physiology ; Electroretinography ; Eye and associated structures. Visual pathways and centers. Vision ; Fundamental and applied biological sciences. Psychology ; Gene Silencing - physiology ; Immunoenzyme Techniques ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Muscle, Smooth, Vascular - physiology ; Pericytes - physiology ; Retina - physiology ; Retinal Vessels - metabolism ; Vertebrates: nervous system and sense organs</subject><ispartof>Investigative ophthalmology & visual science, 2006-06, Vol.47 (6), p.2693-2700</ispartof><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c319t-c536a9f0476e8534089e12456a878537ae17046288f24e6e87099259c7544ab23</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17801546$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16723488$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TOMASEK, James J</creatorcontrib><creatorcontrib>HAAKSMA, Carol J</creatorcontrib><creatorcontrib>SCHWARTZ, Robert J</creatorcontrib><creatorcontrib>VUONG, Duc T</creatorcontrib><creatorcontrib>ZHANG, Sarah X</creatorcontrib><creatorcontrib>ASH, John D</creatorcontrib><creatorcontrib>MA, Jian-Xing</creatorcontrib><creatorcontrib>AL-UBAIDI, Muayyad R</creatorcontrib><title>Deletion of smooth muscle α-actin alters blood-retina barrier permeability and retinal function</title><title>Investigative ophthalmology & visual science</title><addtitle>Invest Ophthalmol Vis Sci</addtitle><description>Vascular smooth muscle (SM) cells and pericytes are essential for normal vascular development. SM alpha-actin null mice were used to determine whether vascular SM and pericyte contractile functions, and not merely their presence, are necessary for vascular development, normal blood-retina barrier (BRB) permeability, and retinal function.
Age-matched SM alpha-actin null and wild-type mice were analyzed. Retinal structure, vascular pattern, and SM cell and pericyte distribution were analyzed histologically. Retinal vascular permeability (RVP) was measured with the Evans blue dye method. Electroretinography (ERG) was performed to evaluate retinal function.
Deletion of SM alpha-actin did not result in any alterations in retinal morphology, vascular pattern, or SM cell and pericyte ensheathing of vessels in SM alpha-actin null mice. A significant increase in RVP was observed in SM alpha-actin null mice at both postnatal day (P)50 and P75 (P<0.05 and P<0.001, respectively). ERG analysis demonstrated a significant reduction in both rod and cone function in SM alpha-actin null mice at P22, P45, and P75 (P<0.01 at all ages).
These results demonstrate that SM alpha-actin in SM cells and pericytes is not necessary for the formation of a normal retinal vascular pattern; however, SM alpha-actin is necessary for SM cells and pericytes to interact with endothelial cells to form a fully functional BRB. These results are important in understanding the role of contractile gene expression in the maintenance and function of the BRB and may provide a model for studying pathologic conditions, such as diabetes, that alter the function of this barrier.</description><subject>Actins - physiology</subject><subject>Albumins - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood-Retinal Barrier - physiology</subject><subject>Blotting, Western</subject><subject>Capillary Permeability - physiology</subject><subject>Electroretinography</subject><subject>Eye and associated structures. Visual pathways and centers. Vision</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Silencing - physiology</subject><subject>Immunoenzyme Techniques</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Knockout</subject><subject>Muscle, Smooth, Vascular - physiology</subject><subject>Pericytes - physiology</subject><subject>Retina - physiology</subject><subject>Retinal Vessels - metabolism</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0146-0404</issn><issn>1552-5783</issn><issn>1552-5783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0M1KxDAUBeAgijOO7lxLNroyY5ImTbqU8RcG3Oi6pplbjKTNmLTCPJYv4jPZMgVX4ZLvngsHoXNGl4zl6saF77SkkjBeqAM0Z1JyIpXODtGcMpETKqiYoZOUPinljHF6jGbDHs-E1nP0fgceOhdaHGqcmhC6D9z0yXrAvz_E2M612PgOYsKVD2FD4qBbgysTo4OItxAbMJXzrtth027w_t_jum_tmHuKjmrjE5xN7wK9Pdy_rp7I-uXxeXW7JjZjRUeszHJT1FSoHLTMBNUFMC5kbrQaZmWAKSpyrnXNBQxG0aLgsrBKCmEqni3Q1T53G8NXD6krG5cseG9aCH0qc00Zlyob4PUe2hhSilCX2-gaE3clo-XYaDk2WlJZjo0O_GLK7asGNv94qnAAlxMwyRpfR9Nal_6dGg5LkWd_KYd_JQ</recordid><startdate>20060601</startdate><enddate>20060601</enddate><creator>TOMASEK, James J</creator><creator>HAAKSMA, Carol J</creator><creator>SCHWARTZ, Robert J</creator><creator>VUONG, Duc T</creator><creator>ZHANG, Sarah X</creator><creator>ASH, John D</creator><creator>MA, Jian-Xing</creator><creator>AL-UBAIDI, Muayyad R</creator><general>Association for Research in Vision and Ophtalmology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060601</creationdate><title>Deletion of smooth muscle α-actin alters blood-retina barrier permeability and retinal function</title><author>TOMASEK, James J ; HAAKSMA, Carol J ; SCHWARTZ, Robert J ; VUONG, Duc T ; ZHANG, Sarah X ; ASH, John D ; MA, Jian-Xing ; AL-UBAIDI, Muayyad R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c319t-c536a9f0476e8534089e12456a878537ae17046288f24e6e87099259c7544ab23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Actins - physiology</topic><topic>Albumins - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood-Retinal Barrier - physiology</topic><topic>Blotting, Western</topic><topic>Capillary Permeability - physiology</topic><topic>Electroretinography</topic><topic>Eye and associated structures. Visual pathways and centers. Vision</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Silencing - physiology</topic><topic>Immunoenzyme Techniques</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Knockout</topic><topic>Muscle, Smooth, Vascular - physiology</topic><topic>Pericytes - physiology</topic><topic>Retina - physiology</topic><topic>Retinal Vessels - metabolism</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TOMASEK, James J</creatorcontrib><creatorcontrib>HAAKSMA, Carol J</creatorcontrib><creatorcontrib>SCHWARTZ, Robert J</creatorcontrib><creatorcontrib>VUONG, Duc T</creatorcontrib><creatorcontrib>ZHANG, Sarah X</creatorcontrib><creatorcontrib>ASH, John D</creatorcontrib><creatorcontrib>MA, Jian-Xing</creatorcontrib><creatorcontrib>AL-UBAIDI, Muayyad R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Investigative ophthalmology & visual science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TOMASEK, James J</au><au>HAAKSMA, Carol J</au><au>SCHWARTZ, Robert J</au><au>VUONG, Duc T</au><au>ZHANG, Sarah X</au><au>ASH, John D</au><au>MA, Jian-Xing</au><au>AL-UBAIDI, Muayyad R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Deletion of smooth muscle α-actin alters blood-retina barrier permeability and retinal function</atitle><jtitle>Investigative ophthalmology & visual science</jtitle><addtitle>Invest Ophthalmol Vis Sci</addtitle><date>2006-06-01</date><risdate>2006</risdate><volume>47</volume><issue>6</issue><spage>2693</spage><epage>2700</epage><pages>2693-2700</pages><issn>0146-0404</issn><issn>1552-5783</issn><eissn>1552-5783</eissn><coden>IOVSDA</coden><abstract>Vascular smooth muscle (SM) cells and pericytes are essential for normal vascular development. SM alpha-actin null mice were used to determine whether vascular SM and pericyte contractile functions, and not merely their presence, are necessary for vascular development, normal blood-retina barrier (BRB) permeability, and retinal function.
Age-matched SM alpha-actin null and wild-type mice were analyzed. Retinal structure, vascular pattern, and SM cell and pericyte distribution were analyzed histologically. Retinal vascular permeability (RVP) was measured with the Evans blue dye method. Electroretinography (ERG) was performed to evaluate retinal function.
Deletion of SM alpha-actin did not result in any alterations in retinal morphology, vascular pattern, or SM cell and pericyte ensheathing of vessels in SM alpha-actin null mice. A significant increase in RVP was observed in SM alpha-actin null mice at both postnatal day (P)50 and P75 (P<0.05 and P<0.001, respectively). ERG analysis demonstrated a significant reduction in both rod and cone function in SM alpha-actin null mice at P22, P45, and P75 (P<0.01 at all ages).
These results demonstrate that SM alpha-actin in SM cells and pericytes is not necessary for the formation of a normal retinal vascular pattern; however, SM alpha-actin is necessary for SM cells and pericytes to interact with endothelial cells to form a fully functional BRB. These results are important in understanding the role of contractile gene expression in the maintenance and function of the BRB and may provide a model for studying pathologic conditions, such as diabetes, that alter the function of this barrier.</abstract><cop>Rockville, MD</cop><pub>Association for Research in Vision and Ophtalmology</pub><pmid>16723488</pmid><doi>10.1167/iovs.05-1297</doi><tpages>8</tpages></addata></record> |
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| subjects | Actins - physiology Albumins - metabolism Animals Biological and medical sciences Blood-Retinal Barrier - physiology Blotting, Western Capillary Permeability - physiology Electroretinography Eye and associated structures. Visual pathways and centers. Vision Fundamental and applied biological sciences. Psychology Gene Silencing - physiology Immunoenzyme Techniques Mice Mice, Inbred C57BL Mice, Knockout Muscle, Smooth, Vascular - physiology Pericytes - physiology Retina - physiology Retinal Vessels - metabolism Vertebrates: nervous system and sense organs |
| title | Deletion of smooth muscle α-actin alters blood-retina barrier permeability and retinal function |
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