Differential gene silencing induced by short interfering RNA in cultured pine cells associates with the cell cycle phase
The double-stranded short interfering RNA (siRNA) molecules can silence targeted genes through sequence-specific cleavage of the cognate RNA transcript. The rapid adoption of technologies based on this siRNA interference mechanism has been a widely used method to analyze gene function in plants, inv...
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| Veröffentlicht in: | Planta 2006-06, Vol.224 (1), p.53-60, Article 53 |
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| description | The double-stranded short interfering RNA (siRNA) molecules can silence targeted genes through sequence-specific cleavage of the cognate RNA transcript. The rapid adoption of technologies based on this siRNA interference mechanism has been a widely used method to analyze gene function in plants, invertebrates, and mammalian systems. In order to understand the dynamics of siRNA-mediated gene inactivation during cell division, we have investigated the relationship between the cell cycle phase and the post-transcriptional gene silencing mediated by siRNA in gfp transgenic Virginia pine (Pinus virginiana Mill.) cells. Among the different phases of the cell cycle, transgenic cells at the M phase gave 2-3 times lower gfp silencing than those at the G1, S, and G2 phases. The similar results of the siRNA-mediated gfp silencing were obtained in three transgenic cell lines. Differential gfp silencing induced by siRNA has been confirmed by northern blot, laser scanning microscopy, and siRNA analysis. These data suggested that siRNA-mediated gene inactivation is associated with the cell cycle phase in Virginia pine. |
| doi_str_mv | 10.1007/s00425-005-0190-z |
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The rapid adoption of technologies based on this siRNA interference mechanism has been a widely used method to analyze gene function in plants, invertebrates, and mammalian systems. In order to understand the dynamics of siRNA-mediated gene inactivation during cell division, we have investigated the relationship between the cell cycle phase and the post-transcriptional gene silencing mediated by siRNA in gfp transgenic Virginia pine (Pinus virginiana Mill.) cells. Among the different phases of the cell cycle, transgenic cells at the M phase gave 2-3 times lower gfp silencing than those at the G1, S, and G2 phases. The similar results of the siRNA-mediated gfp silencing were obtained in three transgenic cell lines. Differential gfp silencing induced by siRNA has been confirmed by northern blot, laser scanning microscopy, and siRNA analysis. These data suggested that siRNA-mediated gene inactivation is associated with the cell cycle phase in Virginia pine.</description><identifier>ISSN: 0032-0935</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/s00425-005-0190-z</identifier><identifier>PMID: 16341704</identifier><identifier>CODEN: PLANAB</identifier><language>eng</language><publisher>Berlin: Springer-Verlag</publisher><subject>Biological and medical sciences ; Cell culture techniques ; Cell cycle ; Cell Cycle - genetics ; Cell division ; Cell kinetics ; Cell lines ; Cell physiology ; Cells, Cultured ; Cultured cells ; double-stranded RNA ; Fundamental and applied biological sciences. Psychology ; Gene Dosage ; Gene silencing ; Genes ; Green Fluorescent Proteins - genetics ; Green Fluorescent Proteins - metabolism ; Inactivation ; Microscopy, Confocal ; Pine trees ; Pinus - cytology ; Pinus - genetics ; Pinus - metabolism ; Pinus virginiana ; Plant cells ; Plant physiology and development ; Plants, Genetically Modified - cytology ; Plants, Genetically Modified - metabolism ; Proteins ; RNA ; RNA Interference ; RNA, Messenger - metabolism ; RNA, Small Interfering - analysis ; RNA, Small Interfering - metabolism ; Small interfering RNA ; Transgenic animals</subject><ispartof>Planta, 2006-06, Vol.224 (1), p.53-60, Article 53</ispartof><rights>Springer-Verlag Berlin Heidelberg 2006</rights><rights>2006 INIST-CNRS</rights><rights>Springer-Verlag 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c433t-5a29c0f1b84e14b70abca2c0b2bcc3955e03a4452e7670da616109522c1278703</citedby><cites>FETCH-LOGICAL-c433t-5a29c0f1b84e14b70abca2c0b2bcc3955e03a4452e7670da616109522c1278703</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23389411$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23389411$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17824455$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16341704$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tang, W</creatorcontrib><creatorcontrib>Newton, R.J</creatorcontrib><creatorcontrib>Weidner, D.A</creatorcontrib><title>Differential gene silencing induced by short interfering RNA in cultured pine cells associates with the cell cycle phase</title><title>Planta</title><addtitle>Planta</addtitle><description>The double-stranded short interfering RNA (siRNA) molecules can silence targeted genes through sequence-specific cleavage of the cognate RNA transcript. The rapid adoption of technologies based on this siRNA interference mechanism has been a widely used method to analyze gene function in plants, invertebrates, and mammalian systems. In order to understand the dynamics of siRNA-mediated gene inactivation during cell division, we have investigated the relationship between the cell cycle phase and the post-transcriptional gene silencing mediated by siRNA in gfp transgenic Virginia pine (Pinus virginiana Mill.) cells. Among the different phases of the cell cycle, transgenic cells at the M phase gave 2-3 times lower gfp silencing than those at the G1, S, and G2 phases. The similar results of the siRNA-mediated gfp silencing were obtained in three transgenic cell lines. Differential gfp silencing induced by siRNA has been confirmed by northern blot, laser scanning microscopy, and siRNA analysis. These data suggested that siRNA-mediated gene inactivation is associated with the cell cycle phase in Virginia pine.</description><subject>Biological and medical sciences</subject><subject>Cell culture techniques</subject><subject>Cell cycle</subject><subject>Cell Cycle - genetics</subject><subject>Cell division</subject><subject>Cell kinetics</subject><subject>Cell lines</subject><subject>Cell physiology</subject><subject>Cells, Cultured</subject><subject>Cultured cells</subject><subject>double-stranded RNA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Dosage</subject><subject>Gene silencing</subject><subject>Genes</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Inactivation</subject><subject>Microscopy, Confocal</subject><subject>Pine trees</subject><subject>Pinus - cytology</subject><subject>Pinus - genetics</subject><subject>Pinus - metabolism</subject><subject>Pinus virginiana</subject><subject>Plant cells</subject><subject>Plant physiology and development</subject><subject>Plants, Genetically Modified - cytology</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>Proteins</subject><subject>RNA</subject><subject>RNA Interference</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA, Small Interfering - analysis</subject><subject>RNA, Small Interfering - metabolism</subject><subject>Small interfering RNA</subject><subject>Transgenic animals</subject><issn>0032-0935</issn><issn>1432-2048</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkctu1DAUhiMEokPhAVgAFhLsAufYTuwsq3KVKpCAri3H48x4lEkG2xFMn54TZUSlLmBh-fJ_5-LzF8VThDcIoN4mAMmrEoAWNlDe3CtWKAUvOUh9v1gB0BkaUZ0Vj1LaAZCo1MPiDGshUYFcFb_fha7z0Q852J5t_OBZCr0fXBg2LAzryfk1a48sbceY6SH7SPgsfvtyQXfmpj5PkaBDoFjn-z4xm9Logs0-sV8hb1neLgpzR9d7dtja5B8XDzrbJ__ktJ8X1x_e_7j8VF59_fj58uKqdFKIXFaWNw46bLX0KFsFtnWWO2h565xoqsqDsFJW3KtawdrWWCM0FecOudIKxHnxesl7iOPPyads9iHNzdjBj1MytabxVaD_C2JDM4NGEfjyDrgbpzjQJ4zmoFWNqiIIF8jFMaXoO3OIYW_j0SCY2TyzmGeoupnNMzcU8_yUeGr3fn0bcXKLgFcnwCZn-y5a8indckpzGsVcXN0p7kK2OYxDjjb0_2zh2RK5S3mMfzNzIXQjEUl_seidHY3dRKp-_Z0DCkDQEnUj_gDGUMY0</recordid><startdate>20060601</startdate><enddate>20060601</enddate><creator>Tang, W</creator><creator>Newton, R.J</creator><creator>Weidner, D.A</creator><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7TM</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20060601</creationdate><title>Differential gene silencing induced by short interfering RNA in cultured pine cells associates with the cell cycle phase</title><author>Tang, W ; Newton, R.J ; Weidner, D.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c433t-5a29c0f1b84e14b70abca2c0b2bcc3955e03a4452e7670da616109522c1278703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Biological and medical sciences</topic><topic>Cell culture techniques</topic><topic>Cell cycle</topic><topic>Cell Cycle - genetics</topic><topic>Cell division</topic><topic>Cell kinetics</topic><topic>Cell lines</topic><topic>Cell physiology</topic><topic>Cells, Cultured</topic><topic>Cultured cells</topic><topic>double-stranded RNA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Dosage</topic><topic>Gene silencing</topic><topic>Genes</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>Inactivation</topic><topic>Microscopy, Confocal</topic><topic>Pine trees</topic><topic>Pinus - cytology</topic><topic>Pinus - genetics</topic><topic>Pinus - metabolism</topic><topic>Pinus virginiana</topic><topic>Plant cells</topic><topic>Plant physiology and development</topic><topic>Plants, Genetically Modified - cytology</topic><topic>Plants, Genetically Modified - metabolism</topic><topic>Proteins</topic><topic>RNA</topic><topic>RNA Interference</topic><topic>RNA, Messenger - metabolism</topic><topic>RNA, Small Interfering - analysis</topic><topic>RNA, Small Interfering - metabolism</topic><topic>Small interfering RNA</topic><topic>Transgenic animals</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tang, W</creatorcontrib><creatorcontrib>Newton, R.J</creatorcontrib><creatorcontrib>Weidner, D.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Planta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tang, W</au><au>Newton, R.J</au><au>Weidner, D.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential gene silencing induced by short interfering RNA in cultured pine cells associates with the cell cycle phase</atitle><jtitle>Planta</jtitle><addtitle>Planta</addtitle><date>2006-06-01</date><risdate>2006</risdate><volume>224</volume><issue>1</issue><spage>53</spage><epage>60</epage><pages>53-60</pages><artnum>53</artnum><issn>0032-0935</issn><eissn>1432-2048</eissn><coden>PLANAB</coden><abstract>The double-stranded short interfering RNA (siRNA) molecules can silence targeted genes through sequence-specific cleavage of the cognate RNA transcript. The rapid adoption of technologies based on this siRNA interference mechanism has been a widely used method to analyze gene function in plants, invertebrates, and mammalian systems. In order to understand the dynamics of siRNA-mediated gene inactivation during cell division, we have investigated the relationship between the cell cycle phase and the post-transcriptional gene silencing mediated by siRNA in gfp transgenic Virginia pine (Pinus virginiana Mill.) cells. Among the different phases of the cell cycle, transgenic cells at the M phase gave 2-3 times lower gfp silencing than those at the G1, S, and G2 phases. The similar results of the siRNA-mediated gfp silencing were obtained in three transgenic cell lines. Differential gfp silencing induced by siRNA has been confirmed by northern blot, laser scanning microscopy, and siRNA analysis. These data suggested that siRNA-mediated gene inactivation is associated with the cell cycle phase in Virginia pine.</abstract><cop>Berlin</cop><pub>Springer-Verlag</pub><pmid>16341704</pmid><doi>10.1007/s00425-005-0190-z</doi><tpages>8</tpages></addata></record> |
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| subjects | Biological and medical sciences Cell culture techniques Cell cycle Cell Cycle - genetics Cell division Cell kinetics Cell lines Cell physiology Cells, Cultured Cultured cells double-stranded RNA Fundamental and applied biological sciences. Psychology Gene Dosage Gene silencing Genes Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Inactivation Microscopy, Confocal Pine trees Pinus - cytology Pinus - genetics Pinus - metabolism Pinus virginiana Plant cells Plant physiology and development Plants, Genetically Modified - cytology Plants, Genetically Modified - metabolism Proteins RNA RNA Interference RNA, Messenger - metabolism RNA, Small Interfering - analysis RNA, Small Interfering - metabolism Small interfering RNA Transgenic animals |
| title | Differential gene silencing induced by short interfering RNA in cultured pine cells associates with the cell cycle phase |
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