Regioselective Glucosylation of Aromatic Compounds: Screening of a Recombinant Glycosyltransferase Library to Identify Biocatalysts
A novel whole‐cell screen for the identification of new glucosyltransferase (GT) biocatalysts within a recombinant enzyme library was developed. Following biotransformation, levels of D‐glucose were used as a measure of biocatalysis. Twenty five enzymes that transfer D‐glucose to trans‐resveratrol w...
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| Veröffentlicht in: | Angewandte Chemie International Edition 2006-05, Vol.45 (21), p.3534-3538 |
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| container_issue | 21 |
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| container_title | Angewandte Chemie International Edition |
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| creator | Weis, Markus Lim, Eng-Kiat Bruce, Neil Bowles, Dianna |
| description | A novel whole‐cell screen for the identification of new glucosyltransferase (GT) biocatalysts within a recombinant enzyme library was developed. Following biotransformation, levels of D‐glucose were used as a measure of biocatalysis. Twenty five enzymes that transfer D‐glucose to trans‐resveratrol were identified that have the regioselectivity shown in the picture. |
| doi_str_mv | 10.1002/anie.200504505 |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 1433-7851 |
| ispartof | Angewandte Chemie International Edition, 2006-05, Vol.45 (21), p.3534-3538 |
| issn | 1433-7851 1521-3773 |
| language | eng |
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| source | MEDLINE; Wiley Journals |
| subjects | Catalysis Chromatography, High Pressure Liquid Drug Evaluation, Preclinical enzymes Glucose - metabolism Glucosides - chemistry glycosides Glycosylation Glycosyltransferases - genetics Glycosyltransferases - metabolism Hydrocarbons, Aromatic - chemistry Peptide Library Recombinant Proteins - genetics Recombinant Proteins - metabolism regioselectivity screening |
| title | Regioselective Glucosylation of Aromatic Compounds: Screening of a Recombinant Glycosyltransferase Library to Identify Biocatalysts |
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