Regioselective Glucosylation of Aromatic Compounds: Screening of a Recombinant Glycosyltransferase Library to Identify Biocatalysts

A novel whole‐cell screen for the identification of new glucosyltransferase (GT) biocatalysts within a recombinant enzyme library was developed. Following biotransformation, levels of D‐glucose were used as a measure of biocatalysis. Twenty five enzymes that transfer D‐glucose to trans‐resveratrol w...

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Veröffentlicht in:	Angewandte Chemie International Edition 2006-05, Vol.45 (21), p.3534-3538
Hauptverfasser:	Weis, Markus, Lim, Eng-Kiat, Bruce, Neil, Bowles, Dianna
Format:	Artikel
Sprache:	eng
Schlagworte:	Catalysis Chromatography, High Pressure Liquid Drug Evaluation, Preclinical enzymes Glucose - metabolism Glucosides - chemistry glycosides Glycosylation Glycosyltransferases - genetics Glycosyltransferases - metabolism Hydrocarbons, Aromatic - chemistry Peptide Library Recombinant Proteins - genetics Recombinant Proteins - metabolism regioselectivity screening
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Beschreibung
Zusammenfassung:	A novel whole‐cell screen for the identification of new glucosyltransferase (GT) biocatalysts within a recombinant enzyme library was developed. Following biotransformation, levels of D‐glucose were used as a measure of biocatalysis. Twenty five enzymes that transfer D‐glucose to trans‐resveratrol were identified that have the regioselectivity shown in the picture.
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.200504505