POSH, a scaffold protein for JNK signaling, binds to ALG-2 and ALIX in Drosophila
Plenty of SH3s (POSH) functions as a scaffold protein for the Jun N-terminal kinase (JNK) signal transduction pathway, which leads to cell death in mammalian cultured cells and Drosophila. Here, we show that POSH forms a complex with Apoptosis-linked gene-2 (ALG-2) and ALG-2-interacting protein (ALI...
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| Veröffentlicht in: | FEBS letters 2006-05, Vol.580 (13), p.3296-3300 |
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| creator | Tsuda, Manabu Seong, Ki-Hyeon Aigaki, Toshiro |
| description | Plenty of SH3s (POSH) functions as a scaffold protein for the Jun N-terminal kinase (JNK) signal transduction pathway, which leads to cell death in mammalian cultured cells and
Drosophila. Here, we show that POSH forms a complex with Apoptosis-linked gene-2 (ALG-2) and ALG-2-interacting protein (ALIX/AIP1) in a calcium-dependent manner. Overexpression of ALG-2 or ALIX in developing imaginal eye discs resulted in roughened or melanized eyes, respectively. These phenotypes were enhanced by co-overexpression of POSH. We found that overexpression of either gene could induce ectopic JNK activation, suggesting that POSH/ALG-2/ALIX may function together in the regulation of the JNK pathway. |
| doi_str_mv | 10.1016/j.febslet.2006.05.005 |
| format | Article |
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Drosophila. Here, we show that POSH forms a complex with Apoptosis-linked gene-2 (ALG-2) and ALG-2-interacting protein (ALIX/AIP1) in a calcium-dependent manner. Overexpression of ALG-2 or ALIX in developing imaginal eye discs resulted in roughened or melanized eyes, respectively. These phenotypes were enhanced by co-overexpression of POSH. We found that overexpression of either gene could induce ectopic JNK activation, suggesting that POSH/ALG-2/ALIX may function together in the regulation of the JNK pathway.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/j.febslet.2006.05.005</identifier><identifier>PMID: 16698022</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>ALG-2 ; ALG-2-interacting protein X ; ALIX ; Animals ; Apoptosis-linked gene-2 ; c-Jun N-terminal kinase ; Calcium-Binding Proteins - genetics ; Calcium-Binding Proteins - metabolism ; Carrier Proteins - genetics ; Carrier Proteins - metabolism ; Cell death ; Cytoskeletal Proteins - genetics ; Cytoskeletal Proteins - metabolism ; Drosophila ; Drosophila - chemistry ; Drosophila - growth & development ; Drosophila - metabolism ; Drosophila Proteins - genetics ; Drosophila Proteins - metabolism ; Eye - anatomy & histology ; Eye - growth & development ; Eye - metabolism ; Gene Expression Regulation, Developmental ; Immunoprecipitation ; JNK ; MAP Kinase Kinase 4 - metabolism ; Microfilament Proteins - genetics ; Microfilament Proteins - metabolism ; Nerve Tissue Proteins - genetics ; Nerve Tissue Proteins - metabolism ; Plenty of SH3s ; POSH ; Protein Interaction Mapping ; reverse transcription-polymerase chain reaction ; RNA, Messenger - analysis ; RNA, Messenger - metabolism ; RT-PCR ; UAS ; upstream activating sequence</subject><ispartof>FEBS letters, 2006-05, Vol.580 (13), p.3296-3300</ispartof><rights>2006 Federation of European Biochemical Societies</rights><rights>FEBS Letters 580 (2006) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5724-651e0a1f63b317c43c146e249f6c1a81e73fe846e204cda2c307a6af8bb6e0883</citedby><cites>FETCH-LOGICAL-c5724-651e0a1f63b317c43c146e249f6c1a81e73fe846e204cda2c307a6af8bb6e0883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.febslet.2006.05.005$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.febslet.2006.05.005$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,1417,1433,3550,27924,27925,45574,45575,45995,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16698022$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tsuda, Manabu</creatorcontrib><creatorcontrib>Seong, Ki-Hyeon</creatorcontrib><creatorcontrib>Aigaki, Toshiro</creatorcontrib><title>POSH, a scaffold protein for JNK signaling, binds to ALG-2 and ALIX in Drosophila</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Plenty of SH3s (POSH) functions as a scaffold protein for the Jun N-terminal kinase (JNK) signal transduction pathway, which leads to cell death in mammalian cultured cells and
Drosophila. Here, we show that POSH forms a complex with Apoptosis-linked gene-2 (ALG-2) and ALG-2-interacting protein (ALIX/AIP1) in a calcium-dependent manner. Overexpression of ALG-2 or ALIX in developing imaginal eye discs resulted in roughened or melanized eyes, respectively. These phenotypes were enhanced by co-overexpression of POSH. We found that overexpression of either gene could induce ectopic JNK activation, suggesting that POSH/ALG-2/ALIX may function together in the regulation of the JNK pathway.</description><subject>ALG-2</subject><subject>ALG-2-interacting protein X</subject><subject>ALIX</subject><subject>Animals</subject><subject>Apoptosis-linked gene-2</subject><subject>c-Jun N-terminal kinase</subject><subject>Calcium-Binding Proteins - genetics</subject><subject>Calcium-Binding Proteins - metabolism</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - metabolism</subject><subject>Cell death</subject><subject>Cytoskeletal Proteins - genetics</subject><subject>Cytoskeletal Proteins - metabolism</subject><subject>Drosophila</subject><subject>Drosophila - chemistry</subject><subject>Drosophila - growth & development</subject><subject>Drosophila - metabolism</subject><subject>Drosophila Proteins - genetics</subject><subject>Drosophila Proteins - metabolism</subject><subject>Eye - anatomy & histology</subject><subject>Eye - growth & development</subject><subject>Eye - metabolism</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Immunoprecipitation</subject><subject>JNK</subject><subject>MAP Kinase Kinase 4 - metabolism</subject><subject>Microfilament Proteins - genetics</subject><subject>Microfilament Proteins - metabolism</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Plenty of SH3s</subject><subject>POSH</subject><subject>Protein Interaction Mapping</subject><subject>reverse transcription-polymerase chain reaction</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - metabolism</subject><subject>RT-PCR</subject><subject>UAS</subject><subject>upstream activating sequence</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkV1P2zAUhi00RDvgJ2zy1a6acBwnjnM1ASuUUfEhQNqd5TjHnas06eJ0iH-Po1TaJbuyffT4PcePCfnCIGbAxNk6tlj6Gvs4ARAxZDFAdkCmTOY84qmQn8gUgKVRlhd8Qj57v4Zwlqw4IhMmRCEhSabk8eH-aTGjmnqjrW3rim67tkfXUNt29OfdLfVu1ejaNasZLV1Tedq39Hx5HSVUN1XY3fyigf7Rtb7d_na1PiGHVtceT_frMXm5mj9fLqLl_fXN5fkyMlmepJHIGIJmVvCSs9yk3LBUYJIWVhimJcOcW5RDCVJT6cRwyLXQVpalQJCSH5NvY24Y-M8Ofa82zhusa91gu_NKSAAenHwIsiIPoBgSsxE04TG-Q6u2ndvo7k0xUIN0tVZ76WqQriBTY4Ov-wa7coPVv1t7ywFYjMCrq_Ht_1LV1fwieRp-cPhAEKGU8zREfR-jMKj967BT3jhsDFauQ9OrqnUfTPsOND2nlA</recordid><startdate>20060529</startdate><enddate>20060529</enddate><creator>Tsuda, Manabu</creator><creator>Seong, Ki-Hyeon</creator><creator>Aigaki, Toshiro</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7X8</scope></search><sort><creationdate>20060529</creationdate><title>POSH, a scaffold protein for JNK signaling, binds to ALG-2 and ALIX in Drosophila</title><author>Tsuda, Manabu ; Seong, Ki-Hyeon ; Aigaki, Toshiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5724-651e0a1f63b317c43c146e249f6c1a81e73fe846e204cda2c307a6af8bb6e0883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>ALG-2</topic><topic>ALG-2-interacting protein X</topic><topic>ALIX</topic><topic>Animals</topic><topic>Apoptosis-linked gene-2</topic><topic>c-Jun N-terminal kinase</topic><topic>Calcium-Binding Proteins - genetics</topic><topic>Calcium-Binding Proteins - metabolism</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - metabolism</topic><topic>Cell death</topic><topic>Cytoskeletal Proteins - genetics</topic><topic>Cytoskeletal Proteins - metabolism</topic><topic>Drosophila</topic><topic>Drosophila - chemistry</topic><topic>Drosophila - growth & development</topic><topic>Drosophila - metabolism</topic><topic>Drosophila Proteins - genetics</topic><topic>Drosophila Proteins - metabolism</topic><topic>Eye - anatomy & histology</topic><topic>Eye - growth & development</topic><topic>Eye - metabolism</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Immunoprecipitation</topic><topic>JNK</topic><topic>MAP Kinase Kinase 4 - metabolism</topic><topic>Microfilament Proteins - genetics</topic><topic>Microfilament Proteins - metabolism</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Plenty of SH3s</topic><topic>POSH</topic><topic>Protein Interaction Mapping</topic><topic>reverse transcription-polymerase chain reaction</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - metabolism</topic><topic>RT-PCR</topic><topic>UAS</topic><topic>upstream activating sequence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsuda, Manabu</creatorcontrib><creatorcontrib>Seong, Ki-Hyeon</creatorcontrib><creatorcontrib>Aigaki, Toshiro</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsuda, Manabu</au><au>Seong, Ki-Hyeon</au><au>Aigaki, Toshiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>POSH, a scaffold protein for JNK signaling, binds to ALG-2 and ALIX in Drosophila</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2006-05-29</date><risdate>2006</risdate><volume>580</volume><issue>13</issue><spage>3296</spage><epage>3300</epage><pages>3296-3300</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Plenty of SH3s (POSH) functions as a scaffold protein for the Jun N-terminal kinase (JNK) signal transduction pathway, which leads to cell death in mammalian cultured cells and
Drosophila. Here, we show that POSH forms a complex with Apoptosis-linked gene-2 (ALG-2) and ALG-2-interacting protein (ALIX/AIP1) in a calcium-dependent manner. Overexpression of ALG-2 or ALIX in developing imaginal eye discs resulted in roughened or melanized eyes, respectively. These phenotypes were enhanced by co-overexpression of POSH. We found that overexpression of either gene could induce ectopic JNK activation, suggesting that POSH/ALG-2/ALIX may function together in the regulation of the JNK pathway.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>16698022</pmid><doi>10.1016/j.febslet.2006.05.005</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Free Content; Access via Wiley Online Library; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | ALG-2 ALG-2-interacting protein X ALIX Animals Apoptosis-linked gene-2 c-Jun N-terminal kinase Calcium-Binding Proteins - genetics Calcium-Binding Proteins - metabolism Carrier Proteins - genetics Carrier Proteins - metabolism Cell death Cytoskeletal Proteins - genetics Cytoskeletal Proteins - metabolism Drosophila Drosophila - chemistry Drosophila - growth & development Drosophila - metabolism Drosophila Proteins - genetics Drosophila Proteins - metabolism Eye - anatomy & histology Eye - growth & development Eye - metabolism Gene Expression Regulation, Developmental Immunoprecipitation JNK MAP Kinase Kinase 4 - metabolism Microfilament Proteins - genetics Microfilament Proteins - metabolism Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism Plenty of SH3s POSH Protein Interaction Mapping reverse transcription-polymerase chain reaction RNA, Messenger - analysis RNA, Messenger - metabolism RT-PCR UAS upstream activating sequence |
| title | POSH, a scaffold protein for JNK signaling, binds to ALG-2 and ALIX in Drosophila |
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