Protective Effects of Aucubin Isolated from Eucommia ulmoides against UVB-Induced Oxidative Stress in Human Skin Fibroblasts
Ultraviolet-B (UVB) irradiation has been demonstrated to produce reactive oxygen species (ROS) in the cells and skin, which induces the synthesis of matrix metalloproteinases (MMPs), causing skin photoaging. Using the human skin fibroblast HS68 cell line in the present study, we investigated the pho...
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| Veröffentlicht in: | Biological & Pharmaceutical Bulletin 2005, Vol.28(7), pp.1244-1248 |
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| creator | Ho, Jin Nyoung Lee, Yoo Hyun Park, Jong Seok Jun, Woo Jin Kim, Hye Kyung Hong, Bum Shik Shin, Dong Hoon Cho, Hong Yon |
| description | Ultraviolet-B (UVB) irradiation has been demonstrated to produce reactive oxygen species (ROS) in the cells and skin, which induces the synthesis of matrix metalloproteinases (MMPs), causing skin photoaging. Using the human skin fibroblast HS68 cell line in the present study, we investigated the photoprotective effects of aucubin from Eucommia ulmoides. Pretreatment with aucubin significantly inhibited the production of MMP-1 by 57% when compared to the UVB-irradiated cells. Additionally, the senescence-associated β-galactosidase (SA β-gal) activity was markedly decreased in the presence of aucubin, which indicates it as an antiphoto-induced aging compound. As the effect of aucubin was determined against ROS, the inhibited ROS formation and malondialdehyde (MDA) levels, and the increased cell viability and glutathione (GSH) level were observed with aucubin under UVB irradiation. Based upon these results, it was suggested that aucubin might play an important role in the cellular defense mechanism against UV radiation-induced photoaging. An understanding of the antioxidant properties of aucubin could, in part, act to elucidate its protective mechanism on the human skin photoaging. |
| doi_str_mv | 10.1248/bpb.28.1244 |
| format | Article |
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Using the human skin fibroblast HS68 cell line in the present study, we investigated the photoprotective effects of aucubin from Eucommia ulmoides. Pretreatment with aucubin significantly inhibited the production of MMP-1 by 57% when compared to the UVB-irradiated cells. Additionally, the senescence-associated β-galactosidase (SA β-gal) activity was markedly decreased in the presence of aucubin, which indicates it as an antiphoto-induced aging compound. As the effect of aucubin was determined against ROS, the inhibited ROS formation and malondialdehyde (MDA) levels, and the increased cell viability and glutathione (GSH) level were observed with aucubin under UVB irradiation. Based upon these results, it was suggested that aucubin might play an important role in the cellular defense mechanism against UV radiation-induced photoaging. An understanding of the antioxidant properties of aucubin could, in part, act to elucidate its protective mechanism on the human skin photoaging.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.28.1244</identifier><identifier>PMID: 15997107</identifier><language>eng</language><publisher>Japan: The Pharmaceutical Society of Japan</publisher><subject>aucubin ; beta-Glucosidase - metabolism ; Eucommia ulmoides ; Eucommiaceae - chemistry ; Fibroblasts - drug effects ; Fibroblasts - enzymology ; Fibroblasts - metabolism ; Free Radicals ; Glucosides - isolation & purification ; Glucosides - pharmacology ; Glutathione - metabolism ; Humans ; Hydrogen Peroxide - pharmacology ; Iridoid Glucosides ; Iridoids - isolation & purification ; Iridoids - pharmacology ; Matrix Metalloproteinase 1 - biosynthesis ; Oxidative Stress ; Penicillamine - analogs & derivatives ; Penicillamine - pharmacology ; photoaging ; Skin - cytology ; Skin - drug effects ; Skin - enzymology ; Skin - metabolism ; ultraviolet B (UVB) ; Ultraviolet Rays</subject><ispartof>Biological and Pharmaceutical Bulletin, 2005, Vol.28(7), pp.1244-1248</ispartof><rights>2005 The Pharmaceutical Society of Japan</rights><rights>Copyright Japan Science and Technology Agency 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c689t-e00e3c2235c2e06e3af85b7e1f68fd8e52fdd239418d385fe74a1a35b04b51193</citedby><cites>FETCH-LOGICAL-c689t-e00e3c2235c2e06e3af85b7e1f68fd8e52fdd239418d385fe74a1a35b04b51193</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15997107$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ho, Jin Nyoung</creatorcontrib><creatorcontrib>Lee, Yoo Hyun</creatorcontrib><creatorcontrib>Park, Jong Seok</creatorcontrib><creatorcontrib>Jun, Woo Jin</creatorcontrib><creatorcontrib>Kim, Hye Kyung</creatorcontrib><creatorcontrib>Hong, Bum Shik</creatorcontrib><creatorcontrib>Shin, Dong Hoon</creatorcontrib><creatorcontrib>Cho, Hong Yon</creatorcontrib><creatorcontrib>Korea University</creatorcontrib><creatorcontrib>cDepartment of Food and Nutrition</creatorcontrib><creatorcontrib>Korea Food and Drug Administration</creatorcontrib><creatorcontrib>aDepartment of Food Technology</creatorcontrib><creatorcontrib>dDepartment of Food and Biotechnology</creatorcontrib><creatorcontrib>bDivision of Food Microbiology</creatorcontrib><creatorcontrib>Han-Seo University</creatorcontrib><creatorcontrib>Graduated School of Life Sciences and Biotechnology</creatorcontrib><creatorcontrib>Chonnam National University</creatorcontrib><title>Protective Effects of Aucubin Isolated from Eucommia ulmoides against UVB-Induced Oxidative Stress in Human Skin Fibroblasts</title><title>Biological & Pharmaceutical Bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>Ultraviolet-B (UVB) irradiation has been demonstrated to produce reactive oxygen species (ROS) in the cells and skin, which induces the synthesis of matrix metalloproteinases (MMPs), causing skin photoaging. Using the human skin fibroblast HS68 cell line in the present study, we investigated the photoprotective effects of aucubin from Eucommia ulmoides. Pretreatment with aucubin significantly inhibited the production of MMP-1 by 57% when compared to the UVB-irradiated cells. Additionally, the senescence-associated β-galactosidase (SA β-gal) activity was markedly decreased in the presence of aucubin, which indicates it as an antiphoto-induced aging compound. As the effect of aucubin was determined against ROS, the inhibited ROS formation and malondialdehyde (MDA) levels, and the increased cell viability and glutathione (GSH) level were observed with aucubin under UVB irradiation. Based upon these results, it was suggested that aucubin might play an important role in the cellular defense mechanism against UV radiation-induced photoaging. An understanding of the antioxidant properties of aucubin could, in part, act to elucidate its protective mechanism on the human skin photoaging.</description><subject>aucubin</subject><subject>beta-Glucosidase - metabolism</subject><subject>Eucommia ulmoides</subject><subject>Eucommiaceae - chemistry</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - enzymology</subject><subject>Fibroblasts - metabolism</subject><subject>Free Radicals</subject><subject>Glucosides - isolation & purification</subject><subject>Glucosides - pharmacology</subject><subject>Glutathione - metabolism</subject><subject>Humans</subject><subject>Hydrogen Peroxide - pharmacology</subject><subject>Iridoid Glucosides</subject><subject>Iridoids - isolation & purification</subject><subject>Iridoids - pharmacology</subject><subject>Matrix Metalloproteinase 1 - biosynthesis</subject><subject>Oxidative Stress</subject><subject>Penicillamine - analogs & derivatives</subject><subject>Penicillamine - pharmacology</subject><subject>photoaging</subject><subject>Skin - cytology</subject><subject>Skin - drug effects</subject><subject>Skin - enzymology</subject><subject>Skin - metabolism</subject><subject>ultraviolet B (UVB)</subject><subject>Ultraviolet Rays</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkd9rFDEQx4Mo9qw--S4BwRfZmh-b2-ybtVzbg0KFWl9Dkp3UnLubM9lIC_7x5m6vFnzJfGE--czAIPSWkhPKavnJbM0Jk7tcP0MLyuumEoyK52hBWiqrJRXyCL1KaUMIaQjjL9ERFW3bUNIs0J-vMUxgJ_8b8Mq5khIODp9mm40f8TqFXk_QYRfDgFfZhmHwGud-CL6DhPWd9mOa8O33L9V67LIt6PW97_ReeDNFSAkXz2Ue9IhvfpZ47k0MptdpSq_RC6f7BG8O9Rjdnq--nV1WV9cX67PTq8ouZTtVQAhwyxgXlgFZAtdOCtMAdUvpOgmCua5jvK2p7LgUDppaU82FIbURlLb8GH2YvdsYfmVIkxp8stD3eoSQk1pKQpigpIDv_wM3Icex7KZoXbe8FlKwQn2cKRtDShGc2kY_6PigKFG7k6hyEsXkLteFfndwZjNA98QeblCAixkoXW91H8bej_A02abG-NAHxQgRqmwqSaMIY3v9fl6xyFaIYvo8mzZp0nfwb5SOk7c9PK7VzM_--2PL_tBRwcj_Aq9MtNs</recordid><startdate>20050701</startdate><enddate>20050701</enddate><creator>Ho, Jin Nyoung</creator><creator>Lee, Yoo Hyun</creator><creator>Park, Jong Seok</creator><creator>Jun, Woo Jin</creator><creator>Kim, Hye Kyung</creator><creator>Hong, Bum Shik</creator><creator>Shin, Dong Hoon</creator><creator>Cho, Hong Yon</creator><general>The Pharmaceutical Society of Japan</general><general>Pharmaceutical Society of Japan</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20050701</creationdate><title>Protective Effects of Aucubin Isolated from Eucommia ulmoides against UVB-Induced Oxidative Stress in Human Skin Fibroblasts</title><author>Ho, Jin Nyoung ; Lee, Yoo Hyun ; Park, Jong Seok ; Jun, Woo Jin ; Kim, Hye Kyung ; Hong, Bum Shik ; Shin, Dong Hoon ; Cho, Hong Yon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c689t-e00e3c2235c2e06e3af85b7e1f68fd8e52fdd239418d385fe74a1a35b04b51193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>aucubin</topic><topic>beta-Glucosidase - metabolism</topic><topic>Eucommia ulmoides</topic><topic>Eucommiaceae - chemistry</topic><topic>Fibroblasts - drug effects</topic><topic>Fibroblasts - enzymology</topic><topic>Fibroblasts - metabolism</topic><topic>Free Radicals</topic><topic>Glucosides - isolation & purification</topic><topic>Glucosides - pharmacology</topic><topic>Glutathione - metabolism</topic><topic>Humans</topic><topic>Hydrogen Peroxide - pharmacology</topic><topic>Iridoid Glucosides</topic><topic>Iridoids - isolation & purification</topic><topic>Iridoids - pharmacology</topic><topic>Matrix Metalloproteinase 1 - biosynthesis</topic><topic>Oxidative Stress</topic><topic>Penicillamine - analogs & derivatives</topic><topic>Penicillamine - pharmacology</topic><topic>photoaging</topic><topic>Skin - cytology</topic><topic>Skin - drug effects</topic><topic>Skin - enzymology</topic><topic>Skin - metabolism</topic><topic>ultraviolet B (UVB)</topic><topic>Ultraviolet Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ho, Jin Nyoung</creatorcontrib><creatorcontrib>Lee, Yoo Hyun</creatorcontrib><creatorcontrib>Park, Jong Seok</creatorcontrib><creatorcontrib>Jun, Woo Jin</creatorcontrib><creatorcontrib>Kim, Hye Kyung</creatorcontrib><creatorcontrib>Hong, Bum Shik</creatorcontrib><creatorcontrib>Shin, Dong Hoon</creatorcontrib><creatorcontrib>Cho, Hong Yon</creatorcontrib><creatorcontrib>Korea University</creatorcontrib><creatorcontrib>cDepartment of Food and Nutrition</creatorcontrib><creatorcontrib>Korea Food and Drug Administration</creatorcontrib><creatorcontrib>aDepartment of Food Technology</creatorcontrib><creatorcontrib>dDepartment of Food and Biotechnology</creatorcontrib><creatorcontrib>bDivision of Food Microbiology</creatorcontrib><creatorcontrib>Han-Seo University</creatorcontrib><creatorcontrib>Graduated School of Life Sciences and Biotechnology</creatorcontrib><creatorcontrib>Chonnam National University</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biological & Pharmaceutical Bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ho, Jin Nyoung</au><au>Lee, Yoo Hyun</au><au>Park, Jong Seok</au><au>Jun, Woo Jin</au><au>Kim, Hye Kyung</au><au>Hong, Bum Shik</au><au>Shin, Dong Hoon</au><au>Cho, Hong Yon</au><aucorp>Korea University</aucorp><aucorp>cDepartment of Food and Nutrition</aucorp><aucorp>Korea Food and Drug Administration</aucorp><aucorp>aDepartment of Food Technology</aucorp><aucorp>dDepartment of Food and Biotechnology</aucorp><aucorp>bDivision of Food Microbiology</aucorp><aucorp>Han-Seo University</aucorp><aucorp>Graduated School of Life Sciences and Biotechnology</aucorp><aucorp>Chonnam National University</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective Effects of Aucubin Isolated from Eucommia ulmoides against UVB-Induced Oxidative Stress in Human Skin Fibroblasts</atitle><jtitle>Biological & Pharmaceutical Bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>2005-07-01</date><risdate>2005</risdate><volume>28</volume><issue>7</issue><spage>1244</spage><epage>1248</epage><pages>1244-1248</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>Ultraviolet-B (UVB) irradiation has been demonstrated to produce reactive oxygen species (ROS) in the cells and skin, which induces the synthesis of matrix metalloproteinases (MMPs), causing skin photoaging. Using the human skin fibroblast HS68 cell line in the present study, we investigated the photoprotective effects of aucubin from Eucommia ulmoides. Pretreatment with aucubin significantly inhibited the production of MMP-1 by 57% when compared to the UVB-irradiated cells. Additionally, the senescence-associated β-galactosidase (SA β-gal) activity was markedly decreased in the presence of aucubin, which indicates it as an antiphoto-induced aging compound. As the effect of aucubin was determined against ROS, the inhibited ROS formation and malondialdehyde (MDA) levels, and the increased cell viability and glutathione (GSH) level were observed with aucubin under UVB irradiation. Based upon these results, it was suggested that aucubin might play an important role in the cellular defense mechanism against UV radiation-induced photoaging. An understanding of the antioxidant properties of aucubin could, in part, act to elucidate its protective mechanism on the human skin photoaging.</abstract><cop>Japan</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>15997107</pmid><doi>10.1248/bpb.28.1244</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | aucubin beta-Glucosidase - metabolism Eucommia ulmoides Eucommiaceae - chemistry Fibroblasts - drug effects Fibroblasts - enzymology Fibroblasts - metabolism Free Radicals Glucosides - isolation & purification Glucosides - pharmacology Glutathione - metabolism Humans Hydrogen Peroxide - pharmacology Iridoid Glucosides Iridoids - isolation & purification Iridoids - pharmacology Matrix Metalloproteinase 1 - biosynthesis Oxidative Stress Penicillamine - analogs & derivatives Penicillamine - pharmacology photoaging Skin - cytology Skin - drug effects Skin - enzymology Skin - metabolism ultraviolet B (UVB) Ultraviolet Rays |
| title | Protective Effects of Aucubin Isolated from Eucommia ulmoides against UVB-Induced Oxidative Stress in Human Skin Fibroblasts |
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