A robust, streamlined, and reproducible method for proteomic analysis of serum by delipidation, albumin and IgG depletion, and two-dimensional gel electrophoresis

Serum is a readily available source for diagnostic assays, but the identification of disease‐specific serum biomarkers has been impeded by the dominance of human serum albumin and immunoglobulins (Igs) in the serum proteome. There is a need to reduce the technical variation in serum processing and a...

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Bibliographische Detailangaben
Veröffentlicht in:	Proteomics (Weinheim) 2005-07, Vol.5 (10), p.2656-2664
Hauptverfasser:	Fu, Qin, Garnham, Christopher P., Elliott, Steven T., Bovenkamp, Diane E., Van Eyk, Jennifer E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Albumin Analytical, structural and metabolic biochemistry Biological and medical sciences Blood Proteins - chemistry Blood Proteins - isolation & purification Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Humans Image Processing, Computer-Assisted Immunoglobulin Immunoglobulin G - blood Immunoglobulin G - isolation & purification Lipids - blood Lipids - isolation & purification Miscellaneous Proteins Proteome Reproducibility of Results Serum Serum Albumin - isolation & purification Two-dimensional gel electrophoresis Two-dimensional gel image analysis
Online-Zugang:	Volltext
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