Efficient biosynthesis of d-allose from d-psicose by cross-linked recombinant l-rhamnose isomerase: Separation of product by ethanol crystallization
Mass production of a rare aldohexose d-allose from d-psicose was achieved in a batch reaction by crude recombinant l-rhamnose isomerase ( l-RhI) cross-linked with glutaraldehyde. The d-psicose substrate was, in turn, mass produced from a naturally abundant ketohexose d-fructose by immobilized recomb...
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| Veröffentlicht in: | Journal of bioscience and bioengineering 2006-04, Vol.101 (4), p.340-345 |
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| container_title | Journal of bioscience and bioengineering |
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| creator | Menavuvu, Buetusiwa Thomas Poonperm, Wayoon Leang, Khim Noguchi, Naoki Okada, Hiromi Morimoto, Kenji Granström, Tom Birger Takada, Goro Izumori, Ken |
| description | Mass production of a rare aldohexose
d-allose from
d-psicose was achieved in a batch reaction by crude recombinant
l-rhamnose isomerase (
l-RhI) cross-linked with glutaraldehyde. The
d-psicose substrate was, in turn, mass produced from a naturally abundant ketohexose
d-fructose by immobilized recombinant
d-tagatose 3-epimerase (
d-TE). At an equilibrium state, 25% of
d-psicose was isomerized to
d-allose, that is, 25 g of
d-allose was obtained from 100 g of
d-psicose. The
d-allose product was easily separated and crystallized from the reaction mixture that contains 25%
d-allose, 8%
d-altrose and 67%
d-psicose using ethanol. Empirically, approximately 338 g, that is, 90% of a theoretical overall yield for the purification of pure
d-allose crystals was produced from 1.5 kg of
d-psicose within 30 d using a constructed bioreactor. The cross-linked enzyme had an operative half-life of two months after repeated usages. |
| doi_str_mv | 10.1263/jbb.101.340 |
| format | Article |
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d-allose from
d-psicose was achieved in a batch reaction by crude recombinant
l-rhamnose isomerase (
l-RhI) cross-linked with glutaraldehyde. The
d-psicose substrate was, in turn, mass produced from a naturally abundant ketohexose
d-fructose by immobilized recombinant
d-tagatose 3-epimerase (
d-TE). At an equilibrium state, 25% of
d-psicose was isomerized to
d-allose, that is, 25 g of
d-allose was obtained from 100 g of
d-psicose. The
d-allose product was easily separated and crystallized from the reaction mixture that contains 25%
d-allose, 8%
d-altrose and 67%
d-psicose using ethanol. Empirically, approximately 338 g, that is, 90% of a theoretical overall yield for the purification of pure
d-allose crystals was produced from 1.5 kg of
d-psicose within 30 d using a constructed bioreactor. The cross-linked enzyme had an operative half-life of two months after repeated usages.</description><identifier>ISSN: 1389-1723</identifier><identifier>EISSN: 1347-4421</identifier><identifier>DOI: 10.1263/jbb.101.340</identifier><identifier>PMID: 16716943</identifier><language>eng</language><publisher>Amsterdarm: Elsevier B.V</publisher><subject>Aldose-Ketose Isomerases - chemistry ; Biological and medical sciences ; Biotechnology ; Biotechnology - methods ; Cell-Free System ; Cross-Linking Reagents - pharmacology ; Crystallization ; d-allose ; d-psicose ; Dose-Response Relationship, Drug ; Escherichia coli - metabolism ; Ethanol - chemistry ; Fructose - biosynthesis ; Fundamental and applied biological sciences. Psychology ; Glucose - biosynthesis ; Glutaral - chemistry ; Hydrogen-Ion Concentration ; l-rhamnose isomerase ; Models, Chemical ; rare sugar ; Recombinant Proteins - chemistry ; Rhamnose - chemistry</subject><ispartof>Journal of bioscience and bioengineering, 2006-04, Vol.101 (4), p.340-345</ispartof><rights>2006 The Society for Biotechnology, Japan</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c566t-cec991972fceede9619d062a6a724db024665e5bbf6407a628c9a7cdc2e3c69f3</citedby><cites>FETCH-LOGICAL-c566t-cec991972fceede9619d062a6a724db024665e5bbf6407a628c9a7cdc2e3c69f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1263/jbb.101.340$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17732008$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16716943$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Menavuvu, Buetusiwa Thomas</creatorcontrib><creatorcontrib>Poonperm, Wayoon</creatorcontrib><creatorcontrib>Leang, Khim</creatorcontrib><creatorcontrib>Noguchi, Naoki</creatorcontrib><creatorcontrib>Okada, Hiromi</creatorcontrib><creatorcontrib>Morimoto, Kenji</creatorcontrib><creatorcontrib>Granström, Tom Birger</creatorcontrib><creatorcontrib>Takada, Goro</creatorcontrib><creatorcontrib>Izumori, Ken</creatorcontrib><title>Efficient biosynthesis of d-allose from d-psicose by cross-linked recombinant l-rhamnose isomerase: Separation of product by ethanol crystallization</title><title>Journal of bioscience and bioengineering</title><addtitle>J Biosci Bioeng</addtitle><description>Mass production of a rare aldohexose
d-allose from
d-psicose was achieved in a batch reaction by crude recombinant
l-rhamnose isomerase (
l-RhI) cross-linked with glutaraldehyde. The
d-psicose substrate was, in turn, mass produced from a naturally abundant ketohexose
d-fructose by immobilized recombinant
d-tagatose 3-epimerase (
d-TE). At an equilibrium state, 25% of
d-psicose was isomerized to
d-allose, that is, 25 g of
d-allose was obtained from 100 g of
d-psicose. The
d-allose product was easily separated and crystallized from the reaction mixture that contains 25%
d-allose, 8%
d-altrose and 67%
d-psicose using ethanol. Empirically, approximately 338 g, that is, 90% of a theoretical overall yield for the purification of pure
d-allose crystals was produced from 1.5 kg of
d-psicose within 30 d using a constructed bioreactor. The cross-linked enzyme had an operative half-life of two months after repeated usages.</description><subject>Aldose-Ketose Isomerases - chemistry</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Biotechnology - methods</subject><subject>Cell-Free System</subject><subject>Cross-Linking Reagents - pharmacology</subject><subject>Crystallization</subject><subject>d-allose</subject><subject>d-psicose</subject><subject>Dose-Response Relationship, Drug</subject><subject>Escherichia coli - metabolism</subject><subject>Ethanol - chemistry</subject><subject>Fructose - biosynthesis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose - biosynthesis</subject><subject>Glutaral - chemistry</subject><subject>Hydrogen-Ion Concentration</subject><subject>l-rhamnose isomerase</subject><subject>Models, Chemical</subject><subject>rare sugar</subject><subject>Recombinant Proteins - chemistry</subject><subject>Rhamnose - chemistry</subject><issn>1389-1723</issn><issn>1347-4421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkT1rHDEQhpeQEDtOqvRhm6Qxe5G0u9IpnTHOBxhcOKmFPkacHO3qotEZLr_DPzha34GbgKuZgYd3hnma5j0lK8p4__nOmBUldNUP5EVzSvtBdMPA6MulX8uOCtafNG8Q7wihggj6ujmhXFAuh_60ebjyPtgAc2lNSLifywYwYJt86zodY0JofU5TnbYY7DKafWtzQuximH-DazPYNJkw65oRu7zR07xgAdMEWSN8aW9hq7MuIc1L7jYnt7NlyYGy0XOKNW-PpW4Lfx-pt80rryPCu2M9a359vfp5-b27vvn24_LiurMj56WzYKWkUjBvARxITqUjnGmuBRucIWzgfITRGM8HIjRnayu1sM4y6C2Xvj9rPh1y60l_doBFTQEtxKhnSDtUXEjJRzI-C1JBhRxGXsHzA_j4oQxebXOYdN4rStRiS1Vbtaeq2qr0h2Pszkzgntijngp8PAIarY4-69kGfOKE6Bkh68qNBw7qt-4DZIWLUwsuVDtFuRT-e8A_jhezDg</recordid><startdate>20060401</startdate><enddate>20060401</enddate><creator>Menavuvu, Buetusiwa Thomas</creator><creator>Poonperm, Wayoon</creator><creator>Leang, Khim</creator><creator>Noguchi, Naoki</creator><creator>Okada, Hiromi</creator><creator>Morimoto, Kenji</creator><creator>Granström, Tom Birger</creator><creator>Takada, Goro</creator><creator>Izumori, Ken</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20060401</creationdate><title>Efficient biosynthesis of d-allose from d-psicose by cross-linked recombinant l-rhamnose isomerase: Separation of product by ethanol crystallization</title><author>Menavuvu, Buetusiwa Thomas ; Poonperm, Wayoon ; Leang, Khim ; Noguchi, Naoki ; Okada, Hiromi ; Morimoto, Kenji ; Granström, Tom Birger ; Takada, Goro ; Izumori, Ken</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c566t-cec991972fceede9619d062a6a724db024665e5bbf6407a628c9a7cdc2e3c69f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Aldose-Ketose Isomerases - chemistry</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Biotechnology - methods</topic><topic>Cell-Free System</topic><topic>Cross-Linking Reagents - pharmacology</topic><topic>Crystallization</topic><topic>d-allose</topic><topic>d-psicose</topic><topic>Dose-Response Relationship, Drug</topic><topic>Escherichia coli - metabolism</topic><topic>Ethanol - chemistry</topic><topic>Fructose - biosynthesis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose - biosynthesis</topic><topic>Glutaral - chemistry</topic><topic>Hydrogen-Ion Concentration</topic><topic>l-rhamnose isomerase</topic><topic>Models, Chemical</topic><topic>rare sugar</topic><topic>Recombinant Proteins - chemistry</topic><topic>Rhamnose - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Menavuvu, Buetusiwa Thomas</creatorcontrib><creatorcontrib>Poonperm, Wayoon</creatorcontrib><creatorcontrib>Leang, Khim</creatorcontrib><creatorcontrib>Noguchi, Naoki</creatorcontrib><creatorcontrib>Okada, Hiromi</creatorcontrib><creatorcontrib>Morimoto, Kenji</creatorcontrib><creatorcontrib>Granström, Tom Birger</creatorcontrib><creatorcontrib>Takada, Goro</creatorcontrib><creatorcontrib>Izumori, Ken</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of bioscience and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Menavuvu, Buetusiwa Thomas</au><au>Poonperm, Wayoon</au><au>Leang, Khim</au><au>Noguchi, Naoki</au><au>Okada, Hiromi</au><au>Morimoto, Kenji</au><au>Granström, Tom Birger</au><au>Takada, Goro</au><au>Izumori, Ken</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Efficient biosynthesis of d-allose from d-psicose by cross-linked recombinant l-rhamnose isomerase: Separation of product by ethanol crystallization</atitle><jtitle>Journal of bioscience and bioengineering</jtitle><addtitle>J Biosci Bioeng</addtitle><date>2006-04-01</date><risdate>2006</risdate><volume>101</volume><issue>4</issue><spage>340</spage><epage>345</epage><pages>340-345</pages><issn>1389-1723</issn><eissn>1347-4421</eissn><abstract>Mass production of a rare aldohexose
d-allose from
d-psicose was achieved in a batch reaction by crude recombinant
l-rhamnose isomerase (
l-RhI) cross-linked with glutaraldehyde. The
d-psicose substrate was, in turn, mass produced from a naturally abundant ketohexose
d-fructose by immobilized recombinant
d-tagatose 3-epimerase (
d-TE). At an equilibrium state, 25% of
d-psicose was isomerized to
d-allose, that is, 25 g of
d-allose was obtained from 100 g of
d-psicose. The
d-allose product was easily separated and crystallized from the reaction mixture that contains 25%
d-allose, 8%
d-altrose and 67%
d-psicose using ethanol. Empirically, approximately 338 g, that is, 90% of a theoretical overall yield for the purification of pure
d-allose crystals was produced from 1.5 kg of
d-psicose within 30 d using a constructed bioreactor. The cross-linked enzyme had an operative half-life of two months after repeated usages.</abstract><cop>Amsterdarm</cop><pub>Elsevier B.V</pub><pmid>16716943</pmid><doi>10.1263/jbb.101.340</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1389-1723 |
| ispartof | Journal of bioscience and bioengineering, 2006-04, Vol.101 (4), p.340-345 |
| issn | 1389-1723 1347-4421 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67996505 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Aldose-Ketose Isomerases - chemistry Biological and medical sciences Biotechnology Biotechnology - methods Cell-Free System Cross-Linking Reagents - pharmacology Crystallization d-allose d-psicose Dose-Response Relationship, Drug Escherichia coli - metabolism Ethanol - chemistry Fructose - biosynthesis Fundamental and applied biological sciences. Psychology Glucose - biosynthesis Glutaral - chemistry Hydrogen-Ion Concentration l-rhamnose isomerase Models, Chemical rare sugar Recombinant Proteins - chemistry Rhamnose - chemistry |
| title | Efficient biosynthesis of d-allose from d-psicose by cross-linked recombinant l-rhamnose isomerase: Separation of product by ethanol crystallization |
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