Expression of thick filament proteins during ontogenesis of the mussel Mytilus trossulus (Mollusca: Bivalvia)
The appearance of thick filament proteins organized into supramolecular complexes was studied by SDS-PAGE and Western-blot analysis at different developmental stages of the mussel Mytilus trossulus. Paramyosin appeared at the egg stage, while twitchin and myorod appeared at the blastula stage (12 h...
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| Veröffentlicht in: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2006-06, Vol.144 (2), p.238-244 |
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| container_title | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
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| creator | Odintsova, N. Dyachuk, V. Kiselev, K. Shelud'ko, N. |
| description | The appearance of thick filament proteins organized into supramolecular complexes was studied by SDS-PAGE and Western-blot analysis at different developmental stages of the mussel
Mytilus trossulus. Paramyosin appeared at the egg stage, while twitchin and myorod appeared at the blastula stage (12 h after fertilization). In addition, RT-PCR analysis showed that the twitchin genes were expressed starting from the blastula stage. Thus, the proteins forming thick filaments of the contractile apparatus of mussel muscles are expressed long before the formation of the first well-organized muscle system of the veliger larvae (55 h). Further, the ratios actin/myosin heavy chain (MHC) and paramyosin/MHC at the veliger stage (96 h) distinctly differed from those in the adult mussel. |
| doi_str_mv | 10.1016/j.cbpb.2006.03.002 |
| format | Article |
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Mytilus trossulus. Paramyosin appeared at the egg stage, while twitchin and myorod appeared at the blastula stage (12 h after fertilization). In addition, RT-PCR analysis showed that the twitchin genes were expressed starting from the blastula stage. Thus, the proteins forming thick filaments of the contractile apparatus of mussel muscles are expressed long before the formation of the first well-organized muscle system of the veliger larvae (55 h). Further, the ratios actin/myosin heavy chain (MHC) and paramyosin/MHC at the veliger stage (96 h) distinctly differed from those in the adult mussel.</description><identifier>ISSN: 1096-4959</identifier><identifier>EISSN: 1879-1107</identifier><identifier>DOI: 10.1016/j.cbpb.2006.03.002</identifier><identifier>PMID: 16626989</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Animals ; Bivalvia ; Embryo, Nonmammalian ; Gene Expression ; Larva ; Life Cycle Stages ; Marine ; Mollusc ; Mussel ; Myogenesis ; Myorod ; Myosin ; Myosins - metabolism ; Mytilus - embryology ; Mytilus - growth & development ; Mytilus trossulus ; Paramyosin ; Thick filament ; Time ; Tropomyosin - metabolism ; Twitchin</subject><ispartof>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2006-06, Vol.144 (2), p.238-244</ispartof><rights>2006 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-95d7362c94700f77ba1ba40cd543fcda09967fb5d7643a47c6c0560e522eece23</citedby><cites>FETCH-LOGICAL-c451t-95d7362c94700f77ba1ba40cd543fcda09967fb5d7643a47c6c0560e522eece23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.cbpb.2006.03.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16626989$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Odintsova, N.</creatorcontrib><creatorcontrib>Dyachuk, V.</creatorcontrib><creatorcontrib>Kiselev, K.</creatorcontrib><creatorcontrib>Shelud'ko, N.</creatorcontrib><title>Expression of thick filament proteins during ontogenesis of the mussel Mytilus trossulus (Mollusca: Bivalvia)</title><title>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology</title><addtitle>Comp Biochem Physiol B Biochem Mol Biol</addtitle><description>The appearance of thick filament proteins organized into supramolecular complexes was studied by SDS-PAGE and Western-blot analysis at different developmental stages of the mussel
Mytilus trossulus. Paramyosin appeared at the egg stage, while twitchin and myorod appeared at the blastula stage (12 h after fertilization). In addition, RT-PCR analysis showed that the twitchin genes were expressed starting from the blastula stage. Thus, the proteins forming thick filaments of the contractile apparatus of mussel muscles are expressed long before the formation of the first well-organized muscle system of the veliger larvae (55 h). Further, the ratios actin/myosin heavy chain (MHC) and paramyosin/MHC at the veliger stage (96 h) distinctly differed from those in the adult mussel.</description><subject>Animals</subject><subject>Bivalvia</subject><subject>Embryo, Nonmammalian</subject><subject>Gene Expression</subject><subject>Larva</subject><subject>Life Cycle Stages</subject><subject>Marine</subject><subject>Mollusc</subject><subject>Mussel</subject><subject>Myogenesis</subject><subject>Myorod</subject><subject>Myosin</subject><subject>Myosins - metabolism</subject><subject>Mytilus - embryology</subject><subject>Mytilus - growth & development</subject><subject>Mytilus trossulus</subject><subject>Paramyosin</subject><subject>Thick filament</subject><subject>Time</subject><subject>Tropomyosin - metabolism</subject><subject>Twitchin</subject><issn>1096-4959</issn><issn>1879-1107</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkTtv3DAQhIkgRvxI_kAKg1UQF1KWlEQegzSJ4RdgI01SExS1cniWxDNJHex_bwp3gLu4mi2-HWBmCPnMoGTAxLd1adtNW3IAUUJVAvB35IitpCoYA_k-36BEUatGHZLjGNcA1YpV7AM5ZEJwoVbqiIwXT5uAMTo_Ud_T9M_ZB9q7wYw4JboJPqGbIu3m4KZ76qfk73HC6OKORjrOMeJA756TG-ZIU_Axzsv19c4PWa35Tn-5rRm2zpx9JAe9GSJ-2usJ-Xt58ef8urj9fXVz_vO2sHXDUqGaTlaCW1VLgF7K1rDW1GC7pq562xlQSsi-zZSoK1NLKyw0ArDhHNEir07Il51vDvA4Y0x6dNHiMJgJ_Ry1kErVjDdvgkzmPiWsMsh3oF0CBuz1JrjRhGfNQC9r6LVe1tDLGhoqndfIT6d797kdsXt92defgR87AHMZW4dBR-twsti5gDbpzrv_-b8AEkCc8g</recordid><startdate>20060601</startdate><enddate>20060601</enddate><creator>Odintsova, N.</creator><creator>Dyachuk, V.</creator><creator>Kiselev, K.</creator><creator>Shelud'ko, N.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TN</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20060601</creationdate><title>Expression of thick filament proteins during ontogenesis of the mussel Mytilus trossulus (Mollusca: Bivalvia)</title><author>Odintsova, N. ; Dyachuk, V. ; Kiselev, K. ; Shelud'ko, N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-95d7362c94700f77ba1ba40cd543fcda09967fb5d7643a47c6c0560e522eece23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Bivalvia</topic><topic>Embryo, Nonmammalian</topic><topic>Gene Expression</topic><topic>Larva</topic><topic>Life Cycle Stages</topic><topic>Marine</topic><topic>Mollusc</topic><topic>Mussel</topic><topic>Myogenesis</topic><topic>Myorod</topic><topic>Myosin</topic><topic>Myosins - metabolism</topic><topic>Mytilus - embryology</topic><topic>Mytilus - growth & development</topic><topic>Mytilus trossulus</topic><topic>Paramyosin</topic><topic>Thick filament</topic><topic>Time</topic><topic>Tropomyosin - metabolism</topic><topic>Twitchin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Odintsova, N.</creatorcontrib><creatorcontrib>Dyachuk, V.</creatorcontrib><creatorcontrib>Kiselev, K.</creatorcontrib><creatorcontrib>Shelud'ko, N.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oceanic Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Odintsova, N.</au><au>Dyachuk, V.</au><au>Kiselev, K.</au><au>Shelud'ko, N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of thick filament proteins during ontogenesis of the mussel Mytilus trossulus (Mollusca: Bivalvia)</atitle><jtitle>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology</jtitle><addtitle>Comp Biochem Physiol B Biochem Mol Biol</addtitle><date>2006-06-01</date><risdate>2006</risdate><volume>144</volume><issue>2</issue><spage>238</spage><epage>244</epage><pages>238-244</pages><issn>1096-4959</issn><eissn>1879-1107</eissn><abstract>The appearance of thick filament proteins organized into supramolecular complexes was studied by SDS-PAGE and Western-blot analysis at different developmental stages of the mussel
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| ispartof | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2006-06, Vol.144 (2), p.238-244 |
| issn | 1096-4959 1879-1107 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Bivalvia Embryo, Nonmammalian Gene Expression Larva Life Cycle Stages Marine Mollusc Mussel Myogenesis Myorod Myosin Myosins - metabolism Mytilus - embryology Mytilus - growth & development Mytilus trossulus Paramyosin Thick filament Time Tropomyosin - metabolism Twitchin |
| title | Expression of thick filament proteins during ontogenesis of the mussel Mytilus trossulus (Mollusca: Bivalvia) |
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