Effect of Glycosylation on the Function of a Soluble, Recombinant Form of the Transferrin Receptor

Production of the soluble portion of the transferrin receptor (sTFR) by baby hamster kidney (BHK) cells is described, and the effect of glycosylation on the biological function of sTFR is evaluated for the first time. The sTFR (residues 121−760) has three N-linked glycosylation sites (Asn251, Asn317...

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Veröffentlicht in:	Biochemistry (Easton) 2006-05, Vol.45 (21), p.6663-6673
Hauptverfasser:	Byrne, Shaina L, Leverence, Rachael, Klein, Joshua S, Giannetti, Anthony M, Smith, Valerie C, MacGillivray, Ross T. A, Kaltashov, Igor A, Mason, Anne B
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Dimerization DNA Primers Glycosylation Kinetics Protein Binding Receptors, Transferrin - chemistry Receptors, Transferrin - genetics Receptors, Transferrin - metabolism Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism Spectrometry, Mass, Electrospray Ionization Surface Plasmon Resonance Transferrin - metabolism
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