AhSSK1, a novel SKP1‐like protein that interacts with the S‐locus F‐box protein SLF
Summary The S‐locus F‐box (SLF/SFB) protein, recently identified as the pollen determinant of S‐RNase‐based self‐incompatibility (SI) in Solanaceae, Scrophulariaceae and Rosaceae, has been proposed to serve as the subunit of an SCF (SKP1–CUL1–F‐box) ubiquitin ligase and to target its pistil counterp...
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| Veröffentlicht in: | The Plant journal : for cell and molecular biology 2006-06, Vol.46 (5), p.780-793 |
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| container_title | The Plant journal : for cell and molecular biology |
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| creator | Huang, Jian Zhao, Lan Yang, Qiuying Xue, Yongbiao |
| description | Summary
The S‐locus F‐box (SLF/SFB) protein, recently identified as the pollen determinant of S‐RNase‐based self‐incompatibility (SI) in Solanaceae, Scrophulariaceae and Rosaceae, has been proposed to serve as the subunit of an SCF (SKP1–CUL1–F‐box) ubiquitin ligase and to target its pistil counterpart S‐RNase during the SI response. However, the underlying mechanism is still in dispute, and the putative SLF‐binding SKP1‐equivalent protein remains unknown. Here, we report the identification of AhSSK1, Antirrhinum hispanicumSLF‐interacting SKP1‐like1, using a yeast two‐hybrid screen against a pollen cDNA library. GST pull‐down assays confirmed the SSK1–SLF interaction, and showed that AhSSK1 could connect AhSLF to a CUL1‐like protein. AhSSK1, despite having a similar secondary structure to other SKP1‐like proteins, appeared quite distinctive in sequence and unique in a phylogenetic analysis, in which no SSK1 ortholog could be predicted in the sequenced genomes of Arabidopsis and rice. Thus, our results suggest that the pollen‐specific SSK1 could be recruited exclusively as the adaptor of putative SCFSLF in those plants with S‐RNase‐based SI, providing an important clue to dissecting the function of the pollen determinant. |
| doi_str_mv | 10.1111/j.1365-313X.2006.02735.x |
| format | Article |
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The S‐locus F‐box (SLF/SFB) protein, recently identified as the pollen determinant of S‐RNase‐based self‐incompatibility (SI) in Solanaceae, Scrophulariaceae and Rosaceae, has been proposed to serve as the subunit of an SCF (SKP1–CUL1–F‐box) ubiquitin ligase and to target its pistil counterpart S‐RNase during the SI response. However, the underlying mechanism is still in dispute, and the putative SLF‐binding SKP1‐equivalent protein remains unknown. Here, we report the identification of AhSSK1, Antirrhinum hispanicumSLF‐interacting SKP1‐like1, using a yeast two‐hybrid screen against a pollen cDNA library. GST pull‐down assays confirmed the SSK1–SLF interaction, and showed that AhSSK1 could connect AhSLF to a CUL1‐like protein. AhSSK1, despite having a similar secondary structure to other SKP1‐like proteins, appeared quite distinctive in sequence and unique in a phylogenetic analysis, in which no SSK1 ortholog could be predicted in the sequenced genomes of Arabidopsis and rice. Thus, our results suggest that the pollen‐specific SSK1 could be recruited exclusively as the adaptor of putative SCFSLF in those plants with S‐RNase‐based SI, providing an important clue to dissecting the function of the pollen determinant.</description><identifier>ISSN: 0960-7412</identifier><identifier>EISSN: 1365-313X</identifier><identifier>DOI: 10.1111/j.1365-313X.2006.02735.x</identifier><identifier>PMID: 16709194</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Alternative Splicing ; Amino Acid Motifs ; Amino Acid Sequence ; Antirrhinum ; Antirrhinum - genetics ; Antirrhinum - metabolism ; Antirrhinum hispanicum ; Biological and medical sciences ; Chemical control ; Control ; F-Box Proteins - metabolism ; Flowers & plants ; Fundamental and applied biological sciences. Psychology ; Fungal plant pathogens ; F‐box ; Genome, Plant ; Molecular Sequence Data ; Phylogeny ; Phytopathology. Animal pests. Plant and forest protection ; Plant Proteins - chemistry ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Pollen ; Pollen - cytology ; Pollen - metabolism ; Protein Interaction Mapping ; Proteins ; S-Phase Kinase-Associated Proteins - metabolism ; self‐incompatibility ; Sequence Alignment ; Sequence Analysis, Protein ; SKP1 ; SLF ; SSK1 ; Two-Hybrid System Techniques</subject><ispartof>The Plant journal : for cell and molecular biology, 2006-06, Vol.46 (5), p.780-793</ispartof><rights>2006 INIST-CNRS</rights><rights>2006 The Authors Journal compilation 2006 Blackwell Publishing Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5105-9e2b7a96be4ffa0e9669ac8e54d702f4db423ca7aee43775d4cb5da252765beb3</citedby><cites>FETCH-LOGICAL-c5105-9e2b7a96be4ffa0e9669ac8e54d702f4db423ca7aee43775d4cb5da252765beb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-313X.2006.02735.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-313X.2006.02735.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,1432,27922,27923,45572,45573,46407,46831</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17789116$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16709194$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Jian</creatorcontrib><creatorcontrib>Zhao, Lan</creatorcontrib><creatorcontrib>Yang, Qiuying</creatorcontrib><creatorcontrib>Xue, Yongbiao</creatorcontrib><title>AhSSK1, a novel SKP1‐like protein that interacts with the S‐locus F‐box protein SLF</title><title>The Plant journal : for cell and molecular biology</title><addtitle>Plant J</addtitle><description>Summary
The S‐locus F‐box (SLF/SFB) protein, recently identified as the pollen determinant of S‐RNase‐based self‐incompatibility (SI) in Solanaceae, Scrophulariaceae and Rosaceae, has been proposed to serve as the subunit of an SCF (SKP1–CUL1–F‐box) ubiquitin ligase and to target its pistil counterpart S‐RNase during the SI response. However, the underlying mechanism is still in dispute, and the putative SLF‐binding SKP1‐equivalent protein remains unknown. Here, we report the identification of AhSSK1, Antirrhinum hispanicumSLF‐interacting SKP1‐like1, using a yeast two‐hybrid screen against a pollen cDNA library. GST pull‐down assays confirmed the SSK1–SLF interaction, and showed that AhSSK1 could connect AhSLF to a CUL1‐like protein. AhSSK1, despite having a similar secondary structure to other SKP1‐like proteins, appeared quite distinctive in sequence and unique in a phylogenetic analysis, in which no SSK1 ortholog could be predicted in the sequenced genomes of Arabidopsis and rice. Thus, our results suggest that the pollen‐specific SSK1 could be recruited exclusively as the adaptor of putative SCFSLF in those plants with S‐RNase‐based SI, providing an important clue to dissecting the function of the pollen determinant.</description><subject>Alternative Splicing</subject><subject>Amino Acid Motifs</subject><subject>Amino Acid Sequence</subject><subject>Antirrhinum</subject><subject>Antirrhinum - genetics</subject><subject>Antirrhinum - metabolism</subject><subject>Antirrhinum hispanicum</subject><subject>Biological and medical sciences</subject><subject>Chemical control</subject><subject>Control</subject><subject>F-Box Proteins - metabolism</subject><subject>Flowers & plants</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal plant pathogens</subject><subject>F‐box</subject><subject>Genome, Plant</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Plant Proteins - chemistry</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Pollen</subject><subject>Pollen - cytology</subject><subject>Pollen - metabolism</subject><subject>Protein Interaction Mapping</subject><subject>Proteins</subject><subject>S-Phase Kinase-Associated Proteins - metabolism</subject><subject>self‐incompatibility</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, Protein</subject><subject>SKP1</subject><subject>SLF</subject><subject>SSK1</subject><subject>Two-Hybrid System Techniques</subject><issn>0960-7412</issn><issn>1365-313X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkctO3DAUhq2qqExpX6GykNoVCb57vGCBUKcFRgIpVGpXluOcaDLNJBAnMOz6CH3GPgkOMwKpG_DGR_b3H18-hDAlKY3jcJlSrmTCKf-ZMkJUSpjmMl2_QZOnjbdoQowiiRaU7aL3ISwJoZor8Q7tUqWJoUZM0K_jRZad0wPscNPeQo2z80v678_fuvoN-Lpre6ga3C9cj6umh875PuC7ql_ENcDZCLZ-CHgWq7xdPyWy-ewD2ildHeDjdt5DP2Zfr06-J_OLb6cnx_PES0pkYoDl2hmVgyhLR8AoZZyfghSFJqwURS4Y9047AMG1loXwuSwck0wrmUPO99CXTd949s0AoberKnioa9dAOwSrtDGUEfEiGP-DC_kI7v8HLtuha-IjLKNcCCMEj9B0A_muDaGD0l531cp195YSO0qySzu6sKMLO0qyj5LsOkY_bfsP-QqK5-DWSgQ-bwEXvKvLzjW-Cs-c1lNDqYrc0Ya7q2q4f_UF7NXl2VjxB4ZyrlE</recordid><startdate>200606</startdate><enddate>200606</enddate><creator>Huang, Jian</creator><creator>Zhao, Lan</creator><creator>Yang, Qiuying</creator><creator>Xue, Yongbiao</creator><general>Blackwell Publishing Ltd</general><general>Blackwell Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200606</creationdate><title>AhSSK1, a novel SKP1‐like protein that interacts with the S‐locus F‐box protein SLF</title><author>Huang, Jian ; Zhao, Lan ; Yang, Qiuying ; Xue, Yongbiao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5105-9e2b7a96be4ffa0e9669ac8e54d702f4db423ca7aee43775d4cb5da252765beb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Alternative Splicing</topic><topic>Amino Acid Motifs</topic><topic>Amino Acid Sequence</topic><topic>Antirrhinum</topic><topic>Antirrhinum - genetics</topic><topic>Antirrhinum - metabolism</topic><topic>Antirrhinum hispanicum</topic><topic>Biological and medical sciences</topic><topic>Chemical control</topic><topic>Control</topic><topic>F-Box Proteins - metabolism</topic><topic>Flowers & plants</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal plant pathogens</topic><topic>F‐box</topic><topic>Genome, Plant</topic><topic>Molecular Sequence Data</topic><topic>Phylogeny</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Plant Proteins - chemistry</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Pollen</topic><topic>Pollen - cytology</topic><topic>Pollen - metabolism</topic><topic>Protein Interaction Mapping</topic><topic>Proteins</topic><topic>S-Phase Kinase-Associated Proteins - metabolism</topic><topic>self‐incompatibility</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, Protein</topic><topic>SKP1</topic><topic>SLF</topic><topic>SSK1</topic><topic>Two-Hybrid System Techniques</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Jian</creatorcontrib><creatorcontrib>Zhao, Lan</creatorcontrib><creatorcontrib>Yang, Qiuying</creatorcontrib><creatorcontrib>Xue, Yongbiao</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Plant journal : for cell and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Jian</au><au>Zhao, Lan</au><au>Yang, Qiuying</au><au>Xue, Yongbiao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>AhSSK1, a novel SKP1‐like protein that interacts with the S‐locus F‐box protein SLF</atitle><jtitle>The Plant journal : for cell and molecular biology</jtitle><addtitle>Plant J</addtitle><date>2006-06</date><risdate>2006</risdate><volume>46</volume><issue>5</issue><spage>780</spage><epage>793</epage><pages>780-793</pages><issn>0960-7412</issn><eissn>1365-313X</eissn><abstract>Summary
The S‐locus F‐box (SLF/SFB) protein, recently identified as the pollen determinant of S‐RNase‐based self‐incompatibility (SI) in Solanaceae, Scrophulariaceae and Rosaceae, has been proposed to serve as the subunit of an SCF (SKP1–CUL1–F‐box) ubiquitin ligase and to target its pistil counterpart S‐RNase during the SI response. However, the underlying mechanism is still in dispute, and the putative SLF‐binding SKP1‐equivalent protein remains unknown. Here, we report the identification of AhSSK1, Antirrhinum hispanicumSLF‐interacting SKP1‐like1, using a yeast two‐hybrid screen against a pollen cDNA library. GST pull‐down assays confirmed the SSK1–SLF interaction, and showed that AhSSK1 could connect AhSLF to a CUL1‐like protein. AhSSK1, despite having a similar secondary structure to other SKP1‐like proteins, appeared quite distinctive in sequence and unique in a phylogenetic analysis, in which no SSK1 ortholog could be predicted in the sequenced genomes of Arabidopsis and rice. Thus, our results suggest that the pollen‐specific SSK1 could be recruited exclusively as the adaptor of putative SCFSLF in those plants with S‐RNase‐based SI, providing an important clue to dissecting the function of the pollen determinant.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>16709194</pmid><doi>10.1111/j.1365-313X.2006.02735.x</doi><tpages>14</tpages></addata></record> |
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| ispartof | The Plant journal : for cell and molecular biology, 2006-06, Vol.46 (5), p.780-793 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Wiley Free Content; IngentaConnect Free/Open Access Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Alternative Splicing Amino Acid Motifs Amino Acid Sequence Antirrhinum Antirrhinum - genetics Antirrhinum - metabolism Antirrhinum hispanicum Biological and medical sciences Chemical control Control F-Box Proteins - metabolism Flowers & plants Fundamental and applied biological sciences. Psychology Fungal plant pathogens F‐box Genome, Plant Molecular Sequence Data Phylogeny Phytopathology. Animal pests. Plant and forest protection Plant Proteins - chemistry Plant Proteins - genetics Plant Proteins - metabolism Pollen Pollen - cytology Pollen - metabolism Protein Interaction Mapping Proteins S-Phase Kinase-Associated Proteins - metabolism self‐incompatibility Sequence Alignment Sequence Analysis, Protein SKP1 SLF SSK1 Two-Hybrid System Techniques |
| title | AhSSK1, a novel SKP1‐like protein that interacts with the S‐locus F‐box protein SLF |
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