Semaphorin 4B interacts with the post-synaptic density protein PSD-95/SAP90 and is recruited to synapses through a C-terminal PDZ-binding motif
The semaphorins are a large family of proteins that act as guidance signals for axons and dendrites. The class 4 semaphorins are integral membrane proteins that are widely expressed throughout the nervous system. Here, we show that a subclass of these semaphorins is characterized by a PDZ-binding mo...
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| Veröffentlicht in: | FEBS letters 2005-07, Vol.579 (17), p.3821-3828 |
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| creator | Burkhardt, Constanze Müller, Myriam Badde, Anja Garner, Craig C. Gundelfinger, Eckart D. Püschel, Andreas W. |
| description | The semaphorins are a large family of proteins that act as guidance signals for axons and dendrites. The class 4 semaphorins are integral membrane proteins that are widely expressed throughout the nervous system. Here, we show that a subclass of these semaphorins is characterized by a PDZ-binding motif at their carboxy-terminus. This sequence mediates the interaction with the post-synaptic density protein PSD-95/SAP90. Co-expression of Sema4B with PSD-95 in COS 7 cells results in the clustering of Sema4B. Sema4B co-localizes with PSD-95 at synaptic contacts between cultured hippocampal neurons. This synaptic localization depends on the presence of the PDZ-binding motif. |
| doi_str_mv | 10.1016/j.febslet.2005.05.079 |
| format | Article |
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The class 4 semaphorins are integral membrane proteins that are widely expressed throughout the nervous system. Here, we show that a subclass of these semaphorins is characterized by a PDZ-binding motif at their carboxy-terminus. This sequence mediates the interaction with the post-synaptic density protein PSD-95/SAP90. Co-expression of Sema4B with PSD-95 in COS 7 cells results in the clustering of Sema4B. Sema4B co-localizes with PSD-95 at synaptic contacts between cultured hippocampal neurons. This synaptic localization depends on the presence of the PDZ-binding motif.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/j.febslet.2005.05.079</identifier><identifier>PMID: 15978582</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Amino Acid Motifs ; Animals ; Axon guidance ; Cercopithecus aethiops ; class ; COS Cells ; days in vitro ; Disks Large Homolog 4 Protein ; DIV ; family ; GAIP interacting protein, C terminus ; GIPC ; glutathione S-transferase ; GST ; Guanylate Kinases ; hemagglutinin ; Hippocampus ; Hippocampus - cytology ; Intracellular Signaling Peptides and Proteins ; Membrane Proteins ; Mice ; N-methyl-d-aspartate ; Nerve Tissue Proteins - analysis ; Nerve Tissue Proteins - genetics ; Nerve Tissue Proteins - metabolism ; nervous system ; Neurons - metabolism ; NMDA ; NMDA receptor 1 ; NR1 ; PDZ ; post-synaptic density ; post-synaptic density-95 ; Protein Structure, Tertiary ; PSD ; PSD-95 ; PSD-95/Discs large/ZO-1 ; SAP90 ; SAP90-PSD95 Associated Proteins ; Semaphorin ; Semaphorins - analysis ; Semaphorins - chemistry ; Semaphorins - metabolism ; subclass ; Synapse ; synapse-associated protein 90 ; Synapses - chemistry ; Synapses - metabolism</subject><ispartof>FEBS letters, 2005-07, Vol.579 (17), p.3821-3828</ispartof><rights>2005 Federation of European Biochemical Societies</rights><rights>FEBS Letters 579 (2005) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4926-c40f1b763524e210a1d9adcc74e1ff65c9ddf050a80444864e47402eff7ab7e43</citedby><cites>FETCH-LOGICAL-c4926-c40f1b763524e210a1d9adcc74e1ff65c9ddf050a80444864e47402eff7ab7e43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.febslet.2005.05.079$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014579305007106$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,1411,1427,3537,27901,27902,45550,45551,46384,46808,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15978582$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Burkhardt, Constanze</creatorcontrib><creatorcontrib>Müller, Myriam</creatorcontrib><creatorcontrib>Badde, Anja</creatorcontrib><creatorcontrib>Garner, Craig C.</creatorcontrib><creatorcontrib>Gundelfinger, Eckart D.</creatorcontrib><creatorcontrib>Püschel, Andreas W.</creatorcontrib><title>Semaphorin 4B interacts with the post-synaptic density protein PSD-95/SAP90 and is recruited to synapses through a C-terminal PDZ-binding motif</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>The semaphorins are a large family of proteins that act as guidance signals for axons and dendrites. The class 4 semaphorins are integral membrane proteins that are widely expressed throughout the nervous system. Here, we show that a subclass of these semaphorins is characterized by a PDZ-binding motif at their carboxy-terminus. This sequence mediates the interaction with the post-synaptic density protein PSD-95/SAP90. Co-expression of Sema4B with PSD-95 in COS 7 cells results in the clustering of Sema4B. Sema4B co-localizes with PSD-95 at synaptic contacts between cultured hippocampal neurons. This synaptic localization depends on the presence of the PDZ-binding motif.</description><subject>Amino Acid Motifs</subject><subject>Animals</subject><subject>Axon guidance</subject><subject>Cercopithecus aethiops</subject><subject>class</subject><subject>COS Cells</subject><subject>days in vitro</subject><subject>Disks Large Homolog 4 Protein</subject><subject>DIV</subject><subject>family</subject><subject>GAIP interacting protein, C terminus</subject><subject>GIPC</subject><subject>glutathione S-transferase</subject><subject>GST</subject><subject>Guanylate Kinases</subject><subject>hemagglutinin</subject><subject>Hippocampus</subject><subject>Hippocampus - cytology</subject><subject>Intracellular Signaling Peptides and Proteins</subject><subject>Membrane Proteins</subject><subject>Mice</subject><subject>N-methyl-d-aspartate</subject><subject>Nerve Tissue Proteins - analysis</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>nervous system</subject><subject>Neurons - metabolism</subject><subject>NMDA</subject><subject>NMDA receptor 1</subject><subject>NR1</subject><subject>PDZ</subject><subject>post-synaptic density</subject><subject>post-synaptic density-95</subject><subject>Protein Structure, Tertiary</subject><subject>PSD</subject><subject>PSD-95</subject><subject>PSD-95/Discs large/ZO-1</subject><subject>SAP90</subject><subject>SAP90-PSD95 Associated Proteins</subject><subject>Semaphorin</subject><subject>Semaphorins - analysis</subject><subject>Semaphorins - chemistry</subject><subject>Semaphorins - metabolism</subject><subject>subclass</subject><subject>Synapse</subject><subject>synapse-associated protein 90</subject><subject>Synapses - chemistry</subject><subject>Synapses - metabolism</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNks1uEzEUhS0EoiHwCCCvEJtJ7Rl7bK9Qm7YUqRKRAhs2lse-0zian2A7VHkKXrmeJhI7inRly9I559r-LkLvKVlQQuvz7aKFJnaQFiUhfDGVUC_QjEpRFRWr5Us0I4SyggtVnaE3MW5JPkuqXqMzypWQXJYz9GcNvdltxuAHzC6xHxIEY1PEDz5tcNoA3o0xFfEwmF3yFjsYok8HvAtjguxZra8Kxc_XFytFsBkc9hEHsGHvEzicRvzkjBBzVhj39xts8LLITXo_mA6vrn4WjR-cH-5xPybfvkWvWtNFeHfa5-jHzfX35W1x9-3L1-XFXWGZKuu8kpY2oq54yaCkxFCnjLNWMKBtW3OrnGsJJ0YSxpisGTDBSAltK0wjgFVz9PGYmx_yaw8x6d5HC11nBhj3UddCKSLLSfjpn8KK8krySlDxbGZJmFS0llnIj0IbxhgDtHoXfG_CQVOiJ7p6q0909URXT5UxztGHU4N904P76zrhzILbo-DBd3D4v1R9c31ZrqdRmSYl_xkRlNQ56vMxCjKF3x6CjtbDYMH5zDdpN_pnbvsI0tjOTw</recordid><startdate>20050704</startdate><enddate>20050704</enddate><creator>Burkhardt, Constanze</creator><creator>Müller, Myriam</creator><creator>Badde, Anja</creator><creator>Garner, Craig C.</creator><creator>Gundelfinger, Eckart D.</creator><creator>Püschel, Andreas W.</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7S9</scope><scope>L.6</scope><scope>7X8</scope></search><sort><creationdate>20050704</creationdate><title>Semaphorin 4B interacts with the post-synaptic density protein PSD-95/SAP90 and is recruited to synapses through a C-terminal PDZ-binding motif</title><author>Burkhardt, Constanze ; Müller, Myriam ; Badde, Anja ; Garner, Craig C. ; Gundelfinger, Eckart D. ; Püschel, Andreas W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4926-c40f1b763524e210a1d9adcc74e1ff65c9ddf050a80444864e47402eff7ab7e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Amino Acid Motifs</topic><topic>Animals</topic><topic>Axon guidance</topic><topic>Cercopithecus aethiops</topic><topic>class</topic><topic>COS Cells</topic><topic>days in vitro</topic><topic>Disks Large Homolog 4 Protein</topic><topic>DIV</topic><topic>family</topic><topic>GAIP interacting protein, C terminus</topic><topic>GIPC</topic><topic>glutathione S-transferase</topic><topic>GST</topic><topic>Guanylate Kinases</topic><topic>hemagglutinin</topic><topic>Hippocampus</topic><topic>Hippocampus - cytology</topic><topic>Intracellular Signaling Peptides and Proteins</topic><topic>Membrane Proteins</topic><topic>Mice</topic><topic>N-methyl-d-aspartate</topic><topic>Nerve Tissue Proteins - analysis</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>nervous system</topic><topic>Neurons - metabolism</topic><topic>NMDA</topic><topic>NMDA receptor 1</topic><topic>NR1</topic><topic>PDZ</topic><topic>post-synaptic density</topic><topic>post-synaptic density-95</topic><topic>Protein Structure, Tertiary</topic><topic>PSD</topic><topic>PSD-95</topic><topic>PSD-95/Discs large/ZO-1</topic><topic>SAP90</topic><topic>SAP90-PSD95 Associated Proteins</topic><topic>Semaphorin</topic><topic>Semaphorins - analysis</topic><topic>Semaphorins - chemistry</topic><topic>Semaphorins - metabolism</topic><topic>subclass</topic><topic>Synapse</topic><topic>synapse-associated protein 90</topic><topic>Synapses - chemistry</topic><topic>Synapses - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Burkhardt, Constanze</creatorcontrib><creatorcontrib>Müller, Myriam</creatorcontrib><creatorcontrib>Badde, Anja</creatorcontrib><creatorcontrib>Garner, Craig C.</creatorcontrib><creatorcontrib>Gundelfinger, Eckart D.</creatorcontrib><creatorcontrib>Püschel, Andreas W.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Burkhardt, Constanze</au><au>Müller, Myriam</au><au>Badde, Anja</au><au>Garner, Craig C.</au><au>Gundelfinger, Eckart D.</au><au>Püschel, Andreas W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Semaphorin 4B interacts with the post-synaptic density protein PSD-95/SAP90 and is recruited to synapses through a C-terminal PDZ-binding motif</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2005-07-04</date><risdate>2005</risdate><volume>579</volume><issue>17</issue><spage>3821</spage><epage>3828</epage><pages>3821-3828</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>The semaphorins are a large family of proteins that act as guidance signals for axons and dendrites. The class 4 semaphorins are integral membrane proteins that are widely expressed throughout the nervous system. Here, we show that a subclass of these semaphorins is characterized by a PDZ-binding motif at their carboxy-terminus. This sequence mediates the interaction with the post-synaptic density protein PSD-95/SAP90. Co-expression of Sema4B with PSD-95 in COS 7 cells results in the clustering of Sema4B. Sema4B co-localizes with PSD-95 at synaptic contacts between cultured hippocampal neurons. This synaptic localization depends on the presence of the PDZ-binding motif.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>15978582</pmid><doi>10.1016/j.febslet.2005.05.079</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 2005-07, Vol.579 (17), p.3821-3828 |
| issn | 0014-5793 1873-3468 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals; Wiley Online Library Free Content; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Amino Acid Motifs Animals Axon guidance Cercopithecus aethiops class COS Cells days in vitro Disks Large Homolog 4 Protein DIV family GAIP interacting protein, C terminus GIPC glutathione S-transferase GST Guanylate Kinases hemagglutinin Hippocampus Hippocampus - cytology Intracellular Signaling Peptides and Proteins Membrane Proteins Mice N-methyl-d-aspartate Nerve Tissue Proteins - analysis Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism nervous system Neurons - metabolism NMDA NMDA receptor 1 NR1 PDZ post-synaptic density post-synaptic density-95 Protein Structure, Tertiary PSD PSD-95 PSD-95/Discs large/ZO-1 SAP90 SAP90-PSD95 Associated Proteins Semaphorin Semaphorins - analysis Semaphorins - chemistry Semaphorins - metabolism subclass Synapse synapse-associated protein 90 Synapses - chemistry Synapses - metabolism |
| title | Semaphorin 4B interacts with the post-synaptic density protein PSD-95/SAP90 and is recruited to synapses through a C-terminal PDZ-binding motif |
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