Comparison of electrospray ionization mass spectrometry and evaporative light scattering detections for the determination of Poloxamer 188 in itraconazole injectable formulation
A high performance liquid chromatography (HPLC) method for Poloxamer 188 using size-exclusion chromatography (SEC) was developed and two different detection mechanisms, evaporative light scattering (ELSD) and electrospray ionization mass spectrometry (ESI-MS), were compared for their quantification...
Gespeichert in:
| Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 2006-06, Vol.41 (3), p.725-730 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 730 |
|---|---|
| container_issue | 3 |
| container_start_page | 725 |
| container_title | Journal of pharmaceutical and biomedical analysis |
| container_volume | 41 |
| creator | Nair, Lakshmy M. Konkel, Jamie Thomas, Mary Koberda, Michael |
| description | A high performance liquid chromatography (HPLC) method for Poloxamer 188 using size-exclusion chromatography (SEC) was developed and two different detection mechanisms, evaporative light scattering (ELSD) and electrospray ionization mass spectrometry (ESI-MS), were compared for their quantification capabilities in itraconazole formulation. Both detection techniques coupled with SEC separation were highly effective for the determination of Poloxamer 188, which is difficult to analyze by other common HPLC methods. As expected, ESI-MS detection provided sensitivity and selectivity superior to ELSD. But since the analyte is an excipient in the formulation, high sensitivity was not required and ELSD's simplicity and ruggedness made it more appropriate for routine analysis of this formulation. |
| doi_str_mv | 10.1016/j.jpba.2005.12.028 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67977593</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0731708506000367</els_id><sourcerecordid>67977593</sourcerecordid><originalsourceid>FETCH-LOGICAL-c384t-645b811c83def033f64ac2b7b1810c5918680899f2cbc091f08d8f37f3be251b3</originalsourceid><addsrcrecordid>eNp9kc1u1DAUhS0EokPhBVggb2CXYMdJ7Ehs0Ki0lSrBAiR2luNct44SO9ieEdO34g3rzIzUHSv_ffdc33MQek9JSQltP4_luPSqrAhpSlqVpBIv0IYKzoqqrX-_RBvCGS04Ec0FehPjSDJIu_o1uqBt3YiaNxv0b-vnRQUbvcPeYJhAp-DjEtQBW-_so0p5wbOKEcfl-DhDCges3IBhrxYfMrEHPNn7h4SjVilBsO4eD5AynosjNj7g9ADHqzBbd9LM7X74yf9VMwRMhcDWYZuC0t6pRz9BPo9ZQfV5mxXm3XSse4teGTVFeHdeL9Gvb1c_tzfF3ffr2-3Xu0IzUaciT9gLSrVgAxjCmGlrpaue91RQopuOilYQ0XWm0r0mHTVEDMIwblgPVUN7dok-nXSX4P_sICY526hhmpQDv4uy5R3nTccyWJ1AnY2LAYxcgp1VOEhK5BqUHOUalFyDkrSSOahc9OGsvutnGJ5Lzslk4OMZUNnUyQTltI3PHOfrCGv3LycOshd7C0FGbcFpGGzI7snB2__94wnsLLcK</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>67977593</pqid></control><display><type>article</type><title>Comparison of electrospray ionization mass spectrometry and evaporative light scattering detections for the determination of Poloxamer 188 in itraconazole injectable formulation</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Nair, Lakshmy M. ; Konkel, Jamie ; Thomas, Mary ; Koberda, Michael</creator><creatorcontrib>Nair, Lakshmy M. ; Konkel, Jamie ; Thomas, Mary ; Koberda, Michael</creatorcontrib><description>A high performance liquid chromatography (HPLC) method for Poloxamer 188 using size-exclusion chromatography (SEC) was developed and two different detection mechanisms, evaporative light scattering (ELSD) and electrospray ionization mass spectrometry (ESI-MS), were compared for their quantification capabilities in itraconazole formulation. Both detection techniques coupled with SEC separation were highly effective for the determination of Poloxamer 188, which is difficult to analyze by other common HPLC methods. As expected, ESI-MS detection provided sensitivity and selectivity superior to ELSD. But since the analyte is an excipient in the formulation, high sensitivity was not required and ELSD's simplicity and ruggedness made it more appropriate for routine analysis of this formulation.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/j.jpba.2005.12.028</identifier><identifier>PMID: 16458475</identifier><identifier>CODEN: JPBADA</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Chromatography, Gel ; ELSD ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Itraconazole ; Itraconazole - analysis ; LC–MS ; Light ; Medical sciences ; Pharmaceutical Preparations - chemistry ; Pharmacology. Drug treatments ; Poloxamer - analysis ; Poloxamer 188 ; Reproducibility of Results ; Scattering, Radiation ; Sensitivity and Specificity ; Spectrometry, Mass, Electrospray Ionization - methods</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2006-06, Vol.41 (3), p.725-730</ispartof><rights>2006 Elsevier B.V.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-645b811c83def033f64ac2b7b1810c5918680899f2cbc091f08d8f37f3be251b3</citedby><cites>FETCH-LOGICAL-c384t-645b811c83def033f64ac2b7b1810c5918680899f2cbc091f08d8f37f3be251b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0731708506000367$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17791863$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16458475$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nair, Lakshmy M.</creatorcontrib><creatorcontrib>Konkel, Jamie</creatorcontrib><creatorcontrib>Thomas, Mary</creatorcontrib><creatorcontrib>Koberda, Michael</creatorcontrib><title>Comparison of electrospray ionization mass spectrometry and evaporative light scattering detections for the determination of Poloxamer 188 in itraconazole injectable formulation</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>A high performance liquid chromatography (HPLC) method for Poloxamer 188 using size-exclusion chromatography (SEC) was developed and two different detection mechanisms, evaporative light scattering (ELSD) and electrospray ionization mass spectrometry (ESI-MS), were compared for their quantification capabilities in itraconazole formulation. Both detection techniques coupled with SEC separation were highly effective for the determination of Poloxamer 188, which is difficult to analyze by other common HPLC methods. As expected, ESI-MS detection provided sensitivity and selectivity superior to ELSD. But since the analyte is an excipient in the formulation, high sensitivity was not required and ELSD's simplicity and ruggedness made it more appropriate for routine analysis of this formulation.</description><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Chromatography, Gel</subject><subject>ELSD</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Itraconazole</subject><subject>Itraconazole - analysis</subject><subject>LC–MS</subject><subject>Light</subject><subject>Medical sciences</subject><subject>Pharmaceutical Preparations - chemistry</subject><subject>Pharmacology. Drug treatments</subject><subject>Poloxamer - analysis</subject><subject>Poloxamer 188</subject><subject>Reproducibility of Results</subject><subject>Scattering, Radiation</subject><subject>Sensitivity and Specificity</subject><subject>Spectrometry, Mass, Electrospray Ionization - methods</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1u1DAUhS0EokPhBVggb2CXYMdJ7Ehs0Ki0lSrBAiR2luNct44SO9ieEdO34g3rzIzUHSv_ffdc33MQek9JSQltP4_luPSqrAhpSlqVpBIv0IYKzoqqrX-_RBvCGS04Ec0FehPjSDJIu_o1uqBt3YiaNxv0b-vnRQUbvcPeYJhAp-DjEtQBW-_so0p5wbOKEcfl-DhDCges3IBhrxYfMrEHPNn7h4SjVilBsO4eD5AynosjNj7g9ADHqzBbd9LM7X74yf9VMwRMhcDWYZuC0t6pRz9BPo9ZQfV5mxXm3XSse4teGTVFeHdeL9Gvb1c_tzfF3ffr2-3Xu0IzUaciT9gLSrVgAxjCmGlrpaue91RQopuOilYQ0XWm0r0mHTVEDMIwblgPVUN7dok-nXSX4P_sICY526hhmpQDv4uy5R3nTccyWJ1AnY2LAYxcgp1VOEhK5BqUHOUalFyDkrSSOahc9OGsvutnGJ5Lzslk4OMZUNnUyQTltI3PHOfrCGv3LycOshd7C0FGbcFpGGzI7snB2__94wnsLLcK</recordid><startdate>20060607</startdate><enddate>20060607</enddate><creator>Nair, Lakshmy M.</creator><creator>Konkel, Jamie</creator><creator>Thomas, Mary</creator><creator>Koberda, Michael</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060607</creationdate><title>Comparison of electrospray ionization mass spectrometry and evaporative light scattering detections for the determination of Poloxamer 188 in itraconazole injectable formulation</title><author>Nair, Lakshmy M. ; Konkel, Jamie ; Thomas, Mary ; Koberda, Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-645b811c83def033f64ac2b7b1810c5918680899f2cbc091f08d8f37f3be251b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Chromatography, Gel</topic><topic>ELSD</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Itraconazole</topic><topic>Itraconazole - analysis</topic><topic>LC–MS</topic><topic>Light</topic><topic>Medical sciences</topic><topic>Pharmaceutical Preparations - chemistry</topic><topic>Pharmacology. Drug treatments</topic><topic>Poloxamer - analysis</topic><topic>Poloxamer 188</topic><topic>Reproducibility of Results</topic><topic>Scattering, Radiation</topic><topic>Sensitivity and Specificity</topic><topic>Spectrometry, Mass, Electrospray Ionization - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nair, Lakshmy M.</creatorcontrib><creatorcontrib>Konkel, Jamie</creatorcontrib><creatorcontrib>Thomas, Mary</creatorcontrib><creatorcontrib>Koberda, Michael</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nair, Lakshmy M.</au><au>Konkel, Jamie</au><au>Thomas, Mary</au><au>Koberda, Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of electrospray ionization mass spectrometry and evaporative light scattering detections for the determination of Poloxamer 188 in itraconazole injectable formulation</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2006-06-07</date><risdate>2006</risdate><volume>41</volume><issue>3</issue><spage>725</spage><epage>730</epage><pages>725-730</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><coden>JPBADA</coden><abstract>A high performance liquid chromatography (HPLC) method for Poloxamer 188 using size-exclusion chromatography (SEC) was developed and two different detection mechanisms, evaporative light scattering (ELSD) and electrospray ionization mass spectrometry (ESI-MS), were compared for their quantification capabilities in itraconazole formulation. Both detection techniques coupled with SEC separation were highly effective for the determination of Poloxamer 188, which is difficult to analyze by other common HPLC methods. As expected, ESI-MS detection provided sensitivity and selectivity superior to ELSD. But since the analyte is an excipient in the formulation, high sensitivity was not required and ELSD's simplicity and ruggedness made it more appropriate for routine analysis of this formulation.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>16458475</pmid><doi>10.1016/j.jpba.2005.12.028</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0731-7085 |
| ispartof | Journal of pharmaceutical and biomedical analysis, 2006-06, Vol.41 (3), p.725-730 |
| issn | 0731-7085 1873-264X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67977593 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Chromatography, Gel ELSD Fundamental and applied biological sciences. Psychology General pharmacology Itraconazole Itraconazole - analysis LC–MS Light Medical sciences Pharmaceutical Preparations - chemistry Pharmacology. Drug treatments Poloxamer - analysis Poloxamer 188 Reproducibility of Results Scattering, Radiation Sensitivity and Specificity Spectrometry, Mass, Electrospray Ionization - methods |
| title | Comparison of electrospray ionization mass spectrometry and evaporative light scattering detections for the determination of Poloxamer 188 in itraconazole injectable formulation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T11%3A01%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparison%20of%20electrospray%20ionization%20mass%20spectrometry%20and%20evaporative%20light%20scattering%20detections%20for%20the%20determination%20of%20Poloxamer%20188%20in%20itraconazole%20injectable%20formulation&rft.jtitle=Journal%20of%20pharmaceutical%20and%20biomedical%20analysis&rft.au=Nair,%20Lakshmy%20M.&rft.date=2006-06-07&rft.volume=41&rft.issue=3&rft.spage=725&rft.epage=730&rft.pages=725-730&rft.issn=0731-7085&rft.eissn=1873-264X&rft.coden=JPBADA&rft_id=info:doi/10.1016/j.jpba.2005.12.028&rft_dat=%3Cproquest_cross%3E67977593%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=67977593&rft_id=info:pmid/16458475&rft_els_id=S0731708506000367&rfr_iscdi=true |