NADPH Oxidase—Dependent Superoxide Production Is Associated With Carotid Intima-Media Thickness in Subjects Free of Clinical Atherosclerotic Disease
OBJECTIVE—Oxidative stress plays a critical role in the pathogenesis of atherosclerosis. The NADPH oxidase constitutes the main source of superoxide in phagocytic and vascular cells. This study aimed to investigate the levels of NADPH oxidase–mediated superoxide production in phagocytic cells and th...
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| Veröffentlicht in: | Arteriosclerosis, thrombosis, and vascular biology thrombosis, and vascular biology, 2005-07, Vol.25 (7), p.1452-1457 |
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| container_title | Arteriosclerosis, thrombosis, and vascular biology |
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| creator | Zalba, Guillermo Beloqui, Oscar San José, Gorka Moreno, María U Fortuño, Ana Díez, Javier |
| description | OBJECTIVE—Oxidative stress plays a critical role in the pathogenesis of atherosclerosis. The NADPH oxidase constitutes the main source of superoxide in phagocytic and vascular cells. This study aimed to investigate the levels of NADPH oxidase–mediated superoxide production in phagocytic cells and the association between phagocytic superoxide production and carotid intima-media thickness (IMT), a surrogate marker of asymptomatic atherosclerosis.
METHODS AND RESULTS—NADPH oxidase–mediated superoxide production was determined by a chemiluminescence assay using lucigenin and associated with IMT for 184 asymptomatic subjects free of overt clinical atherosclerotic disease. Compared with individuals in the lowest tertile of superoxide production, those in the upper tertile (>20 counts/sec) showed significantly higher IMT (P |
| doi_str_mv | 10.1161/01.ATV.0000168411.72483.08 |
| format | Article |
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METHODS AND RESULTS—NADPH oxidase–mediated superoxide production was determined by a chemiluminescence assay using lucigenin and associated with IMT for 184 asymptomatic subjects free of overt clinical atherosclerotic disease. Compared with individuals in the lowest tertile of superoxide production, those in the upper tertile (>20 counts/sec) showed significantly higher IMT (P<0.05). In correlation analysis, a positive relationship was found between superoxide production and carotid IMT. Superoxide production also correlated positively (P<0.05) with body mass index (BMI). In multivariate analysis, the association of superoxide production with carotid IMT remained significant after adjustment for age, sex, systolic blood pressure, BMI, triglycerides, glucose, and smoking.
CONCLUSIONS—In a population sample of adults without clinically overt atherosclerotic disease, increased NADPH oxidase activity was associated with enhanced carotid IMT, suggesting a relationship between phagocytic NADPH oxidase–mediated oxidative stress and the development of atherosclerosis.</description><identifier>ISSN: 1079-5642</identifier><identifier>EISSN: 1524-4636</identifier><identifier>DOI: 10.1161/01.ATV.0000168411.72483.08</identifier><identifier>PMID: 15860744</identifier><identifier>CODEN: ATVBFA</identifier><language>eng</language><publisher>Philadelphia, PA: American Heart Association, Inc</publisher><subject>Atherosclerosis (general aspects, experimental research) ; Biological and medical sciences ; Blood and lymphatic vessels ; Cardiology. Vascular system ; Cardiovascular system ; Carotid Arteries - metabolism ; Carotid Arteries - pathology ; Carotid Artery Diseases - epidemiology ; Carotid Artery Diseases - metabolism ; Carotid Artery Diseases - pathology ; Coronary heart disease ; Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous ; Female ; Heart ; Humans ; Investigative techniques, diagnostic techniques (general aspects) ; Linear Models ; Male ; Medical sciences ; Middle Aged ; NADPH Oxidases - metabolism ; Oxidative Stress ; Phagocytes - enzymology ; Risk Factors ; Superoxides - metabolism ; Tunica Intima - metabolism ; Tunica Intima - pathology ; Tunica Media - metabolism ; Tunica Media - pathology ; Ultrasonic investigative techniques</subject><ispartof>Arteriosclerosis, thrombosis, and vascular biology, 2005-07, Vol.25 (7), p.1452-1457</ispartof><rights>2005 American Heart Association, Inc.</rights><rights>2005 INIST-CNRS</rights><rights>Copyright American Heart Association, Inc. Jul 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5348-453192985d640d0e90e6d60499bc6cb73a582ca3e5af66cc076449f23f2a42583</citedby><cites>FETCH-LOGICAL-c5348-453192985d640d0e90e6d60499bc6cb73a582ca3e5af66cc076449f23f2a42583</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16942906$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15860744$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zalba, Guillermo</creatorcontrib><creatorcontrib>Beloqui, Oscar</creatorcontrib><creatorcontrib>San José, Gorka</creatorcontrib><creatorcontrib>Moreno, María U</creatorcontrib><creatorcontrib>Fortuño, Ana</creatorcontrib><creatorcontrib>Díez, Javier</creatorcontrib><title>NADPH Oxidase—Dependent Superoxide Production Is Associated With Carotid Intima-Media Thickness in Subjects Free of Clinical Atherosclerotic Disease</title><title>Arteriosclerosis, thrombosis, and vascular biology</title><addtitle>Arterioscler Thromb Vasc Biol</addtitle><description>OBJECTIVE—Oxidative stress plays a critical role in the pathogenesis of atherosclerosis. The NADPH oxidase constitutes the main source of superoxide in phagocytic and vascular cells. This study aimed to investigate the levels of NADPH oxidase–mediated superoxide production in phagocytic cells and the association between phagocytic superoxide production and carotid intima-media thickness (IMT), a surrogate marker of asymptomatic atherosclerosis.
METHODS AND RESULTS—NADPH oxidase–mediated superoxide production was determined by a chemiluminescence assay using lucigenin and associated with IMT for 184 asymptomatic subjects free of overt clinical atherosclerotic disease. Compared with individuals in the lowest tertile of superoxide production, those in the upper tertile (>20 counts/sec) showed significantly higher IMT (P<0.05). In correlation analysis, a positive relationship was found between superoxide production and carotid IMT. Superoxide production also correlated positively (P<0.05) with body mass index (BMI). In multivariate analysis, the association of superoxide production with carotid IMT remained significant after adjustment for age, sex, systolic blood pressure, BMI, triglycerides, glucose, and smoking.
CONCLUSIONS—In a population sample of adults without clinically overt atherosclerotic disease, increased NADPH oxidase activity was associated with enhanced carotid IMT, suggesting a relationship between phagocytic NADPH oxidase–mediated oxidative stress and the development of atherosclerosis.</description><subject>Atherosclerosis (general aspects, experimental research)</subject><subject>Biological and medical sciences</subject><subject>Blood and lymphatic vessels</subject><subject>Cardiology. Vascular system</subject><subject>Cardiovascular system</subject><subject>Carotid Arteries - metabolism</subject><subject>Carotid Arteries - pathology</subject><subject>Carotid Artery Diseases - epidemiology</subject><subject>Carotid Artery Diseases - metabolism</subject><subject>Carotid Artery Diseases - pathology</subject><subject>Coronary heart disease</subject><subject>Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous</subject><subject>Female</subject><subject>Heart</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Linear Models</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>NADPH Oxidases - metabolism</subject><subject>Oxidative Stress</subject><subject>Phagocytes - enzymology</subject><subject>Risk Factors</subject><subject>Superoxides - metabolism</subject><subject>Tunica Intima - metabolism</subject><subject>Tunica Intima - pathology</subject><subject>Tunica Media - metabolism</subject><subject>Tunica Media - pathology</subject><subject>Ultrasonic investigative techniques</subject><issn>1079-5642</issn><issn>1524-4636</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkd-KEzEUxgdR3HX1FSQs6N3U_J_Eu6F13cLqLlj1MqSZM0y605maZFi98yHEB_RJTG2hYCAkIb9838n5iuKS4BkhkrzBZFavvsxwHkQqTsisolyxGVaPinMiKC-5ZPJx3uNKl0JyelY8i3GTeU4pflqcEaEkrjg_L35_rBd31-j2u29shD8_fy1gB0MDQ0Kfph2EMV8AugtjM7nkxwEtI6pjHJ23CRr01acOzW0Yk2_Qckh-a8sP0HiLVp139wPEiPyQpdYbcCmiqwCAxhbNez94Z3tUpy6bRNfDXsOhhY-QC3lePGltH-HFcb0oPl-9W82vy5vb98t5fVM6wbgquWBEU61EIzluMGgMspGYa7120q0rZoWizjIQtpXSOVxJznVLWUstp0Kxi-L1QXcXxm8TxGS2PjroezvAOEUjK11Ryvbg5X_gZpzCkGszNHdVaapoht4eIJe_FAO0ZhdyR8IPQ7DZR2cwMTk6c4rO_IvO4L3Dy6PDtN5Cc3p6zCoDr46Ajbl1bbCD8_HESc2pxjJz_MA9jH2CEO_76QGC6cD2qdtbcyaxKCnGAlf5WOZJFfsLm7Cylw</recordid><startdate>200507</startdate><enddate>200507</enddate><creator>Zalba, Guillermo</creator><creator>Beloqui, Oscar</creator><creator>San José, Gorka</creator><creator>Moreno, María U</creator><creator>Fortuño, Ana</creator><creator>Díez, Javier</creator><general>American Heart Association, Inc</general><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>200507</creationdate><title>NADPH Oxidase—Dependent Superoxide Production Is Associated With Carotid Intima-Media Thickness in Subjects Free of Clinical Atherosclerotic Disease</title><author>Zalba, Guillermo ; Beloqui, Oscar ; San José, Gorka ; Moreno, María U ; Fortuño, Ana ; Díez, Javier</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5348-453192985d640d0e90e6d60499bc6cb73a582ca3e5af66cc076449f23f2a42583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Atherosclerosis (general aspects, experimental research)</topic><topic>Biological and medical sciences</topic><topic>Blood and lymphatic vessels</topic><topic>Cardiology. Vascular system</topic><topic>Cardiovascular system</topic><topic>Carotid Arteries - metabolism</topic><topic>Carotid Arteries - pathology</topic><topic>Carotid Artery Diseases - epidemiology</topic><topic>Carotid Artery Diseases - metabolism</topic><topic>Carotid Artery Diseases - pathology</topic><topic>Coronary heart disease</topic><topic>Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous</topic><topic>Female</topic><topic>Heart</topic><topic>Humans</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Linear Models</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>NADPH Oxidases - metabolism</topic><topic>Oxidative Stress</topic><topic>Phagocytes - enzymology</topic><topic>Risk Factors</topic><topic>Superoxides - metabolism</topic><topic>Tunica Intima - metabolism</topic><topic>Tunica Intima - pathology</topic><topic>Tunica Media - metabolism</topic><topic>Tunica Media - pathology</topic><topic>Ultrasonic investigative techniques</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zalba, Guillermo</creatorcontrib><creatorcontrib>Beloqui, Oscar</creatorcontrib><creatorcontrib>San José, Gorka</creatorcontrib><creatorcontrib>Moreno, María U</creatorcontrib><creatorcontrib>Fortuño, Ana</creatorcontrib><creatorcontrib>Díez, Javier</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Arteriosclerosis, thrombosis, and vascular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zalba, Guillermo</au><au>Beloqui, Oscar</au><au>San José, Gorka</au><au>Moreno, María U</au><au>Fortuño, Ana</au><au>Díez, Javier</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>NADPH Oxidase—Dependent Superoxide Production Is Associated With Carotid Intima-Media Thickness in Subjects Free of Clinical Atherosclerotic Disease</atitle><jtitle>Arteriosclerosis, thrombosis, and vascular biology</jtitle><addtitle>Arterioscler Thromb Vasc Biol</addtitle><date>2005-07</date><risdate>2005</risdate><volume>25</volume><issue>7</issue><spage>1452</spage><epage>1457</epage><pages>1452-1457</pages><issn>1079-5642</issn><eissn>1524-4636</eissn><coden>ATVBFA</coden><abstract>OBJECTIVE—Oxidative stress plays a critical role in the pathogenesis of atherosclerosis. The NADPH oxidase constitutes the main source of superoxide in phagocytic and vascular cells. This study aimed to investigate the levels of NADPH oxidase–mediated superoxide production in phagocytic cells and the association between phagocytic superoxide production and carotid intima-media thickness (IMT), a surrogate marker of asymptomatic atherosclerosis.
METHODS AND RESULTS—NADPH oxidase–mediated superoxide production was determined by a chemiluminescence assay using lucigenin and associated with IMT for 184 asymptomatic subjects free of overt clinical atherosclerotic disease. Compared with individuals in the lowest tertile of superoxide production, those in the upper tertile (>20 counts/sec) showed significantly higher IMT (P<0.05). In correlation analysis, a positive relationship was found between superoxide production and carotid IMT. Superoxide production also correlated positively (P<0.05) with body mass index (BMI). In multivariate analysis, the association of superoxide production with carotid IMT remained significant after adjustment for age, sex, systolic blood pressure, BMI, triglycerides, glucose, and smoking.
CONCLUSIONS—In a population sample of adults without clinically overt atherosclerotic disease, increased NADPH oxidase activity was associated with enhanced carotid IMT, suggesting a relationship between phagocytic NADPH oxidase–mediated oxidative stress and the development of atherosclerosis.</abstract><cop>Philadelphia, PA</cop><cop>Hagerstown, MD</cop><pub>American Heart Association, Inc</pub><pmid>15860744</pmid><doi>10.1161/01.ATV.0000168411.72483.08</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Arteriosclerosis, thrombosis, and vascular biology, 2005-07, Vol.25 (7), p.1452-1457 |
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| subjects | Atherosclerosis (general aspects, experimental research) Biological and medical sciences Blood and lymphatic vessels Cardiology. Vascular system Cardiovascular system Carotid Arteries - metabolism Carotid Arteries - pathology Carotid Artery Diseases - epidemiology Carotid Artery Diseases - metabolism Carotid Artery Diseases - pathology Coronary heart disease Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous Female Heart Humans Investigative techniques, diagnostic techniques (general aspects) Linear Models Male Medical sciences Middle Aged NADPH Oxidases - metabolism Oxidative Stress Phagocytes - enzymology Risk Factors Superoxides - metabolism Tunica Intima - metabolism Tunica Intima - pathology Tunica Media - metabolism Tunica Media - pathology Ultrasonic investigative techniques |
| title | NADPH Oxidase—Dependent Superoxide Production Is Associated With Carotid Intima-Media Thickness in Subjects Free of Clinical Atherosclerotic Disease |
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