A defined system to allow skeletal muscle differentiation and subsequent integration with silicon microstructures
This work documents the development of an in vitro cell culture model consisting of a novel serum-free medium and a non-biological growth substrate, N-1[3 (trimethoxysilyl) propyl] diethylenetriamine (DETA), to enable functional myotube integration with cantilevers fabricated using MEMS technology....
Gespeichert in:
| Veröffentlicht in: | Biomaterials 2006-08, Vol.27 (24), p.4374-4380 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 4380 |
|---|---|
| container_issue | 24 |
| container_start_page | 4374 |
| container_title | Biomaterials |
| container_volume | 27 |
| creator | Das, Mainak Gregory, Cassie A. Molnar, Peter Riedel, Lisa M. Wilson, Kerry Hickman, James J. |
| description | This work documents the development of an in vitro cell culture model consisting of a novel serum-free medium and a non-biological growth substrate,
N-1[3 (trimethoxysilyl) propyl] diethylenetriamine (DETA), to enable functional myotube integration with cantilevers fabricated using MEMS technology. This newly developed, defined in vitro model was used to study the differentiation of fetal rat skeletal muscle and it promoted the formation of myotubes from the dissociated rat fetal muscle cells. The myotubes were characterized by morphological analysis, immunocytochemistry and electrophysiology. Further, it was demonstrated that when the dissociated muscle cells were plated on fabricated microcantilevers, the muscle cells aligned along the major axis of the cantilever and formed robust myotubes. This novel system could not only find applications in skeletal muscle differentiation and biocompatibility studies but also in bioartificial muscle engineering, hybrid actuation system development, biorobotics and for a better understanding of myopathies and neuromuscular disorders. |
| doi_str_mv | 10.1016/j.biomaterials.2006.03.046 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67966302</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0142961206002638</els_id><sourcerecordid>19440216</sourcerecordid><originalsourceid>FETCH-LOGICAL-c409t-b65866c997eb755ffbb133dcaa9da46963479cde64179f81513c13f4f3db77093</originalsourceid><addsrcrecordid>eNqNkU2PFCEQhonRuOPqXzDEg7duqYaGwdtm_Uw28aJnQkOhjHT3LtBu9t_LZCbRm55IwfNSBQ8hr4D1wEC-OfRTXGdbMUebSj8wJnvGeybkI7KDvdp3o2bjY7JjIIZOSxguyLNSDqzVTAxPyQVIKRQA35G7K-oxxAU9LQ-l4kzrSm1K6z0tPzFhtYnOW3EJqY8hYMalRlvjulC7tMw2Fbzb2iaNS8Xv-XR0H-sPWmKKrhVzdHktNW-ubhnLc_IktLHxxXm9JN8-vP96_am7-fLx8_XVTecE07Wb5LiX0mmtcFLjGMI0AefeWau9FVJLLpR2HqUApcMeRuAOeBCB-0kppvkleX269zavbcJSzRyLw5TsgutWjFRaSs6Gf4KghWADyAa-PYHH95SMwdzmONv8YICZoxlzMH-bMUczhnHTzLTwy3OXbZrR_4meVTTg3QnA9im_ImZTXMTFoY8ZXTV-jf_T5zfNSal2</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19440216</pqid></control><display><type>article</type><title>A defined system to allow skeletal muscle differentiation and subsequent integration with silicon microstructures</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Das, Mainak ; Gregory, Cassie A. ; Molnar, Peter ; Riedel, Lisa M. ; Wilson, Kerry ; Hickman, James J.</creator><creatorcontrib>Das, Mainak ; Gregory, Cassie A. ; Molnar, Peter ; Riedel, Lisa M. ; Wilson, Kerry ; Hickman, James J.</creatorcontrib><description>This work documents the development of an in vitro cell culture model consisting of a novel serum-free medium and a non-biological growth substrate,
N-1[3 (trimethoxysilyl) propyl] diethylenetriamine (DETA), to enable functional myotube integration with cantilevers fabricated using MEMS technology. This newly developed, defined in vitro model was used to study the differentiation of fetal rat skeletal muscle and it promoted the formation of myotubes from the dissociated rat fetal muscle cells. The myotubes were characterized by morphological analysis, immunocytochemistry and electrophysiology. Further, it was demonstrated that when the dissociated muscle cells were plated on fabricated microcantilevers, the muscle cells aligned along the major axis of the cantilever and formed robust myotubes. This novel system could not only find applications in skeletal muscle differentiation and biocompatibility studies but also in bioartificial muscle engineering, hybrid actuation system development, biorobotics and for a better understanding of myopathies and neuromuscular disorders.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2006.03.046</identifier><identifier>PMID: 16647113</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Animals ; Biocompatible Materials ; Cell Culture Techniques ; Cell Differentiation - physiology ; Cells, Cultured ; Defined system ; Hybrid devices ; MEMS ; Muscle Fibers, Skeletal - cytology ; Muscle Fibers, Skeletal - physiology ; Muscle, Skeletal - cytology ; Muscle, Skeletal - physiology ; Myotubes ; Rats ; Serum-free ; Silicon</subject><ispartof>Biomaterials, 2006-08, Vol.27 (24), p.4374-4380</ispartof><rights>2006 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-b65866c997eb755ffbb133dcaa9da46963479cde64179f81513c13f4f3db77093</citedby><cites>FETCH-LOGICAL-c409t-b65866c997eb755ffbb133dcaa9da46963479cde64179f81513c13f4f3db77093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biomaterials.2006.03.046$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16647113$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Das, Mainak</creatorcontrib><creatorcontrib>Gregory, Cassie A.</creatorcontrib><creatorcontrib>Molnar, Peter</creatorcontrib><creatorcontrib>Riedel, Lisa M.</creatorcontrib><creatorcontrib>Wilson, Kerry</creatorcontrib><creatorcontrib>Hickman, James J.</creatorcontrib><title>A defined system to allow skeletal muscle differentiation and subsequent integration with silicon microstructures</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>This work documents the development of an in vitro cell culture model consisting of a novel serum-free medium and a non-biological growth substrate,
N-1[3 (trimethoxysilyl) propyl] diethylenetriamine (DETA), to enable functional myotube integration with cantilevers fabricated using MEMS technology. This newly developed, defined in vitro model was used to study the differentiation of fetal rat skeletal muscle and it promoted the formation of myotubes from the dissociated rat fetal muscle cells. The myotubes were characterized by morphological analysis, immunocytochemistry and electrophysiology. Further, it was demonstrated that when the dissociated muscle cells were plated on fabricated microcantilevers, the muscle cells aligned along the major axis of the cantilever and formed robust myotubes. This novel system could not only find applications in skeletal muscle differentiation and biocompatibility studies but also in bioartificial muscle engineering, hybrid actuation system development, biorobotics and for a better understanding of myopathies and neuromuscular disorders.</description><subject>Animals</subject><subject>Biocompatible Materials</subject><subject>Cell Culture Techniques</subject><subject>Cell Differentiation - physiology</subject><subject>Cells, Cultured</subject><subject>Defined system</subject><subject>Hybrid devices</subject><subject>MEMS</subject><subject>Muscle Fibers, Skeletal - cytology</subject><subject>Muscle Fibers, Skeletal - physiology</subject><subject>Muscle, Skeletal - cytology</subject><subject>Muscle, Skeletal - physiology</subject><subject>Myotubes</subject><subject>Rats</subject><subject>Serum-free</subject><subject>Silicon</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU2PFCEQhonRuOPqXzDEg7duqYaGwdtm_Uw28aJnQkOhjHT3LtBu9t_LZCbRm55IwfNSBQ8hr4D1wEC-OfRTXGdbMUebSj8wJnvGeybkI7KDvdp3o2bjY7JjIIZOSxguyLNSDqzVTAxPyQVIKRQA35G7K-oxxAU9LQ-l4kzrSm1K6z0tPzFhtYnOW3EJqY8hYMalRlvjulC7tMw2Fbzb2iaNS8Xv-XR0H-sPWmKKrhVzdHktNW-ubhnLc_IktLHxxXm9JN8-vP96_am7-fLx8_XVTecE07Wb5LiX0mmtcFLjGMI0AefeWau9FVJLLpR2HqUApcMeRuAOeBCB-0kppvkleX269zavbcJSzRyLw5TsgutWjFRaSs6Gf4KghWADyAa-PYHH95SMwdzmONv8YICZoxlzMH-bMUczhnHTzLTwy3OXbZrR_4meVTTg3QnA9im_ImZTXMTFoY8ZXTV-jf_T5zfNSal2</recordid><startdate>20060801</startdate><enddate>20060801</enddate><creator>Das, Mainak</creator><creator>Gregory, Cassie A.</creator><creator>Molnar, Peter</creator><creator>Riedel, Lisa M.</creator><creator>Wilson, Kerry</creator><creator>Hickman, James J.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20060801</creationdate><title>A defined system to allow skeletal muscle differentiation and subsequent integration with silicon microstructures</title><author>Das, Mainak ; Gregory, Cassie A. ; Molnar, Peter ; Riedel, Lisa M. ; Wilson, Kerry ; Hickman, James J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-b65866c997eb755ffbb133dcaa9da46963479cde64179f81513c13f4f3db77093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Biocompatible Materials</topic><topic>Cell Culture Techniques</topic><topic>Cell Differentiation - physiology</topic><topic>Cells, Cultured</topic><topic>Defined system</topic><topic>Hybrid devices</topic><topic>MEMS</topic><topic>Muscle Fibers, Skeletal - cytology</topic><topic>Muscle Fibers, Skeletal - physiology</topic><topic>Muscle, Skeletal - cytology</topic><topic>Muscle, Skeletal - physiology</topic><topic>Myotubes</topic><topic>Rats</topic><topic>Serum-free</topic><topic>Silicon</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Das, Mainak</creatorcontrib><creatorcontrib>Gregory, Cassie A.</creatorcontrib><creatorcontrib>Molnar, Peter</creatorcontrib><creatorcontrib>Riedel, Lisa M.</creatorcontrib><creatorcontrib>Wilson, Kerry</creatorcontrib><creatorcontrib>Hickman, James J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Das, Mainak</au><au>Gregory, Cassie A.</au><au>Molnar, Peter</au><au>Riedel, Lisa M.</au><au>Wilson, Kerry</au><au>Hickman, James J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A defined system to allow skeletal muscle differentiation and subsequent integration with silicon microstructures</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2006-08-01</date><risdate>2006</risdate><volume>27</volume><issue>24</issue><spage>4374</spage><epage>4380</epage><pages>4374-4380</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>This work documents the development of an in vitro cell culture model consisting of a novel serum-free medium and a non-biological growth substrate,
N-1[3 (trimethoxysilyl) propyl] diethylenetriamine (DETA), to enable functional myotube integration with cantilevers fabricated using MEMS technology. This newly developed, defined in vitro model was used to study the differentiation of fetal rat skeletal muscle and it promoted the formation of myotubes from the dissociated rat fetal muscle cells. The myotubes were characterized by morphological analysis, immunocytochemistry and electrophysiology. Further, it was demonstrated that when the dissociated muscle cells were plated on fabricated microcantilevers, the muscle cells aligned along the major axis of the cantilever and formed robust myotubes. This novel system could not only find applications in skeletal muscle differentiation and biocompatibility studies but also in bioartificial muscle engineering, hybrid actuation system development, biorobotics and for a better understanding of myopathies and neuromuscular disorders.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>16647113</pmid><doi>10.1016/j.biomaterials.2006.03.046</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0142-9612 |
| ispartof | Biomaterials, 2006-08, Vol.27 (24), p.4374-4380 |
| issn | 0142-9612 1878-5905 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67966302 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Biocompatible Materials Cell Culture Techniques Cell Differentiation - physiology Cells, Cultured Defined system Hybrid devices MEMS Muscle Fibers, Skeletal - cytology Muscle Fibers, Skeletal - physiology Muscle, Skeletal - cytology Muscle, Skeletal - physiology Myotubes Rats Serum-free Silicon |
| title | A defined system to allow skeletal muscle differentiation and subsequent integration with silicon microstructures |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T14%3A16%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20defined%20system%20to%20allow%20skeletal%20muscle%20differentiation%20and%20subsequent%20integration%20with%20silicon%20microstructures&rft.jtitle=Biomaterials&rft.au=Das,%20Mainak&rft.date=2006-08-01&rft.volume=27&rft.issue=24&rft.spage=4374&rft.epage=4380&rft.pages=4374-4380&rft.issn=0142-9612&rft.eissn=1878-5905&rft_id=info:doi/10.1016/j.biomaterials.2006.03.046&rft_dat=%3Cproquest_cross%3E19440216%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19440216&rft_id=info:pmid/16647113&rft_els_id=S0142961206002638&rfr_iscdi=true |