Cultivation of methanotrophic bacteria in opposing gradients of methane and oxygen
Abstract In sediments, methane-oxidizing bacteria live in opposing gradients of methane and oxygen. In such a gradient system, the fluxes of methane and oxygen are controlled by diffusion and consumption rates, and the rate-limiting substrate is maintained at a minimum concentration at the layer of...
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| Veröffentlicht in: | FEMS microbiology ecology 2006-06, Vol.56 (3), p.331-344 |
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| creator | Bussmann, Ingeborg Rahalkar, Monali Schink, Bernhard |
| description | Abstract
In sediments, methane-oxidizing bacteria live in opposing gradients of methane and oxygen. In such a gradient system, the fluxes of methane and oxygen are controlled by diffusion and consumption rates, and the rate-limiting substrate is maintained at a minimum concentration at the layer of consumption. Opposing gradients of methane and oxygen were mimicked in a specific cultivation set-up in which growth of methanotrophic bacteria occurred as a sharp band at either c. 5 or 20 mm below the air-exposed end. Two new strains of methanotrophic bacteria were isolated with this system. One isolate, strain LC 1, belonged to the Methylomonas genus (type I methantroph) and contained soluble methane mono-oxygenase. Another isolate, strain LC 2, was related to the Methylobacter group (type I methantroph), as determined by 16S rRNA gene and pmoA sequence similarities. However, the partial pmoA sequence was only 86% related to cultured Methylobacter species. This strain accumulated significant amounts of formaldehyde in conventional cultivation with methane and oxygen, which may explain why it is preferentially enriched in a gradient cultivation system. |
| doi_str_mv | 10.1111/j.1574-6941.2006.00076.x |
| format | Article |
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In sediments, methane-oxidizing bacteria live in opposing gradients of methane and oxygen. In such a gradient system, the fluxes of methane and oxygen are controlled by diffusion and consumption rates, and the rate-limiting substrate is maintained at a minimum concentration at the layer of consumption. Opposing gradients of methane and oxygen were mimicked in a specific cultivation set-up in which growth of methanotrophic bacteria occurred as a sharp band at either c. 5 or 20 mm below the air-exposed end. Two new strains of methanotrophic bacteria were isolated with this system. One isolate, strain LC 1, belonged to the Methylomonas genus (type I methantroph) and contained soluble methane mono-oxygenase. Another isolate, strain LC 2, was related to the Methylobacter group (type I methantroph), as determined by 16S rRNA gene and pmoA sequence similarities. However, the partial pmoA sequence was only 86% related to cultured Methylobacter species. This strain accumulated significant amounts of formaldehyde in conventional cultivation with methane and oxygen, which may explain why it is preferentially enriched in a gradient cultivation system.</description><identifier>ISSN: 0168-6496</identifier><identifier>EISSN: 1574-6941</identifier><identifier>DOI: 10.1111/j.1574-6941.2006.00076.x</identifier><identifier>PMID: 16689866</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Bacteria ; Bacteriological Techniques - methods ; Bacteriology ; Biological and medical sciences ; Concentration gradient ; Consumption ; Cultivation ; Diffusion rate ; Ecology ; Fluxes ; freshwater sediment ; Fundamental and applied biological sciences. Psychology ; Geologic Sediments - microbiology ; gradient cultivation ; Lake Constance ; Methane ; Methane - metabolism ; Methanotrophic bacteria ; methanotrophs ; Methylobacter ; Methylococcaceae - growth & development ; Methylococcaceae - isolation & purification ; Methylococcaceae - physiology ; Methylomonas ; Microbiology ; Miscellaneous ; Oxidation ; Oxygen ; Oxygen - metabolism ; Oxygen enrichment ; Oxygenase ; RNA, Bacterial - genetics ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; Sediments ; Substrates</subject><ispartof>FEMS microbiology ecology, 2006-06, Vol.56 (3), p.331-344</ispartof><rights>2006 Federation of European Microbiological Societies 2006</rights><rights>2006 INIST-CNRS</rights><rights>2006 Federation of European Microbiological Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5846-b3da61f1a658251a10b785cb689a9b0996f64a90dea31d9cadfcd9d63325cb0f3</citedby><cites>FETCH-LOGICAL-c5846-b3da61f1a658251a10b785cb689a9b0996f64a90dea31d9cadfcd9d63325cb0f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6941.2006.00076.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6941.2006.00076.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17773979$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16689866$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bussmann, Ingeborg</creatorcontrib><creatorcontrib>Rahalkar, Monali</creatorcontrib><creatorcontrib>Schink, Bernhard</creatorcontrib><title>Cultivation of methanotrophic bacteria in opposing gradients of methane and oxygen</title><title>FEMS microbiology ecology</title><addtitle>FEMS Microbiol Ecol</addtitle><description>Abstract
In sediments, methane-oxidizing bacteria live in opposing gradients of methane and oxygen. In such a gradient system, the fluxes of methane and oxygen are controlled by diffusion and consumption rates, and the rate-limiting substrate is maintained at a minimum concentration at the layer of consumption. Opposing gradients of methane and oxygen were mimicked in a specific cultivation set-up in which growth of methanotrophic bacteria occurred as a sharp band at either c. 5 or 20 mm below the air-exposed end. Two new strains of methanotrophic bacteria were isolated with this system. One isolate, strain LC 1, belonged to the Methylomonas genus (type I methantroph) and contained soluble methane mono-oxygenase. Another isolate, strain LC 2, was related to the Methylobacter group (type I methantroph), as determined by 16S rRNA gene and pmoA sequence similarities. However, the partial pmoA sequence was only 86% related to cultured Methylobacter species. This strain accumulated significant amounts of formaldehyde in conventional cultivation with methane and oxygen, which may explain why it is preferentially enriched in a gradient cultivation system.</description><subject>Bacteria</subject><subject>Bacteriological Techniques - methods</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Concentration gradient</subject><subject>Consumption</subject><subject>Cultivation</subject><subject>Diffusion rate</subject><subject>Ecology</subject><subject>Fluxes</subject><subject>freshwater sediment</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Geologic Sediments - microbiology</subject><subject>gradient cultivation</subject><subject>Lake Constance</subject><subject>Methane</subject><subject>Methane - metabolism</subject><subject>Methanotrophic bacteria</subject><subject>methanotrophs</subject><subject>Methylobacter</subject><subject>Methylococcaceae - growth & development</subject><subject>Methylococcaceae - isolation & purification</subject><subject>Methylococcaceae - physiology</subject><subject>Methylomonas</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Oxidation</subject><subject>Oxygen</subject><subject>Oxygen - metabolism</subject><subject>Oxygen enrichment</subject><subject>Oxygenase</subject><subject>RNA, Bacterial - genetics</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>rRNA 16S</subject><subject>Sediments</subject><subject>Substrates</subject><issn>0168-6496</issn><issn>1574-6941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkF2L1DAUhoMo7uyuf0EKonetSdOeNOCNDPshrAjiXofTJJ3N0Glq0u7O_HszzrADimBuEjjPm_PyEJIxWrB0Pq4LVosqB1mxoqQUCkqpgGL7giyeBy_JgjJocqgknJHzGNeUsppX9DU5YwCNbAAW5Pty7if3iJPzQ-a7bGOnBxz8FPz44HTWop5scJi5NB1HH92wylYBjbPDFE8Bm-FgMr_drexwSV512Ef75nhfkPvrqx_L2_zu282X5ee7XNdNBXnLDQLrGELdlDVDRlvR1LpNzVC2VErooEJJjUXOjNRoOm2kAc7LRNGOX5APh3_H4H_ONk5q46K2fZ_q-DkqEBIqVpcJfPcHuPZzGFI3VXIqWENrDolqDpQOPsZgOzUGt8GwU4yqvXW1Vnu5ai9X7a2r39bVNkXfHhfM7caaU_CoOQHvjwBGjX0XcNAunjghBJdCJu7TgXtyvd39dwF1ffU1PVKcH-J-Hv8Rzv9u_wuQBKyC</recordid><startdate>200606</startdate><enddate>200606</enddate><creator>Bussmann, Ingeborg</creator><creator>Rahalkar, Monali</creator><creator>Schink, Bernhard</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200606</creationdate><title>Cultivation of methanotrophic bacteria in opposing gradients of methane and oxygen</title><author>Bussmann, Ingeborg ; Rahalkar, Monali ; Schink, Bernhard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5846-b3da61f1a658251a10b785cb689a9b0996f64a90dea31d9cadfcd9d63325cb0f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Bacteria</topic><topic>Bacteriological Techniques - methods</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Concentration gradient</topic><topic>Consumption</topic><topic>Cultivation</topic><topic>Diffusion rate</topic><topic>Ecology</topic><topic>Fluxes</topic><topic>freshwater sediment</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Geologic Sediments - microbiology</topic><topic>gradient cultivation</topic><topic>Lake Constance</topic><topic>Methane</topic><topic>Methane - metabolism</topic><topic>Methanotrophic bacteria</topic><topic>methanotrophs</topic><topic>Methylobacter</topic><topic>Methylococcaceae - growth & development</topic><topic>Methylococcaceae - isolation & purification</topic><topic>Methylococcaceae - physiology</topic><topic>Methylomonas</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Oxidation</topic><topic>Oxygen</topic><topic>Oxygen - metabolism</topic><topic>Oxygen enrichment</topic><topic>Oxygenase</topic><topic>RNA, Bacterial - genetics</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>rRNA 16S</topic><topic>Sediments</topic><topic>Substrates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bussmann, Ingeborg</creatorcontrib><creatorcontrib>Rahalkar, Monali</creatorcontrib><creatorcontrib>Schink, Bernhard</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology ecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bussmann, Ingeborg</au><au>Rahalkar, Monali</au><au>Schink, Bernhard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cultivation of methanotrophic bacteria in opposing gradients of methane and oxygen</atitle><jtitle>FEMS microbiology ecology</jtitle><addtitle>FEMS Microbiol Ecol</addtitle><date>2006-06</date><risdate>2006</risdate><volume>56</volume><issue>3</issue><spage>331</spage><epage>344</epage><pages>331-344</pages><issn>0168-6496</issn><eissn>1574-6941</eissn><abstract>Abstract
In sediments, methane-oxidizing bacteria live in opposing gradients of methane and oxygen. In such a gradient system, the fluxes of methane and oxygen are controlled by diffusion and consumption rates, and the rate-limiting substrate is maintained at a minimum concentration at the layer of consumption. Opposing gradients of methane and oxygen were mimicked in a specific cultivation set-up in which growth of methanotrophic bacteria occurred as a sharp band at either c. 5 or 20 mm below the air-exposed end. Two new strains of methanotrophic bacteria were isolated with this system. One isolate, strain LC 1, belonged to the Methylomonas genus (type I methantroph) and contained soluble methane mono-oxygenase. Another isolate, strain LC 2, was related to the Methylobacter group (type I methantroph), as determined by 16S rRNA gene and pmoA sequence similarities. However, the partial pmoA sequence was only 86% related to cultured Methylobacter species. This strain accumulated significant amounts of formaldehyde in conventional cultivation with methane and oxygen, which may explain why it is preferentially enriched in a gradient cultivation system.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>16689866</pmid><doi>10.1111/j.1574-6941.2006.00076.x</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford Journals Open Access Collection; MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Bacteria Bacteriological Techniques - methods Bacteriology Biological and medical sciences Concentration gradient Consumption Cultivation Diffusion rate Ecology Fluxes freshwater sediment Fundamental and applied biological sciences. Psychology Geologic Sediments - microbiology gradient cultivation Lake Constance Methane Methane - metabolism Methanotrophic bacteria methanotrophs Methylobacter Methylococcaceae - growth & development Methylococcaceae - isolation & purification Methylococcaceae - physiology Methylomonas Microbiology Miscellaneous Oxidation Oxygen Oxygen - metabolism Oxygen enrichment Oxygenase RNA, Bacterial - genetics RNA, Ribosomal, 16S - genetics rRNA 16S Sediments Substrates |
| title | Cultivation of methanotrophic bacteria in opposing gradients of methane and oxygen |
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