Human endothelial cells allow passage of an archetypal BK virus (BKV) strain : a tool for cultivation and functional studies of natural BKV strains
The ubiquitous human polyomavirus BK (BKV) causes the serious condition BKV-nephropathy in an increasing number of renal-transplant patients. The lack of authentic cell cultures for multiplication of naturally occurring strains has hampered cultivation and functional studies of BKV. Here we demonstr...
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| Veröffentlicht in: | Archives of virology 2005-07, Vol.150 (7), p.1449-1458 |
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| creator | RINALDO, C. Hanssen HANSEN, H TRAAVIK, T |
| description | The ubiquitous human polyomavirus BK (BKV) causes the serious condition BKV-nephropathy in an increasing number of renal-transplant patients. The lack of authentic cell cultures for multiplication of naturally occurring strains has hampered cultivation and functional studies of BKV. Here we demonstrate that the most common urine shed BKV strain, the archetype, multiplies in the human endothelial cell line HUV-EC-C. Additional variants with deletions in the non-coding control region (NCCR) appear upon prolonged propagation. Although the titer produced was low, at the present HUV-EC-C is the only cell line shown to allow propagation of archetypal BKV. HUV-EC-C may therefore be a useful tool for BKV cultivation as well as functional studies. |
| doi_str_mv | 10.1007/s00705-005-0511-3 |
| format | Article |
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HUV-EC-C may therefore be a useful tool for BKV cultivation as well as functional studies.</description><identifier>ISSN: 0304-8608</identifier><identifier>EISSN: 1432-8798</identifier><identifier>DOI: 10.1007/s00705-005-0511-3</identifier><identifier>PMID: 15770354</identifier><language>eng</language><publisher>Wien: Springer</publisher><subject>Biological and medical sciences ; BK virus ; BK Virus - classification ; BK Virus - genetics ; BK Virus - growth & development ; BK Virus - physiology ; Cell culture ; Endothelial Cells - virology ; Fundamental and applied biological sciences. 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Hanssen</creatorcontrib><creatorcontrib>HANSEN, H</creatorcontrib><creatorcontrib>TRAAVIK, T</creatorcontrib><title>Human endothelial cells allow passage of an archetypal BK virus (BKV) strain : a tool for cultivation and functional studies of natural BKV strains</title><title>Archives of virology</title><addtitle>Arch Virol</addtitle><description>The ubiquitous human polyomavirus BK (BKV) causes the serious condition BKV-nephropathy in an increasing number of renal-transplant patients. The lack of authentic cell cultures for multiplication of naturally occurring strains has hampered cultivation and functional studies of BKV. Here we demonstrate that the most common urine shed BKV strain, the archetype, multiplies in the human endothelial cell line HUV-EC-C. Additional variants with deletions in the non-coding control region (NCCR) appear upon prolonged propagation. 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| subjects | Biological and medical sciences BK virus BK Virus - classification BK Virus - genetics BK Virus - growth & development BK Virus - physiology Cell culture Endothelial Cells - virology Fundamental and applied biological sciences. Psychology Genomes Humans Infections Microbiology Miscellaneous Molecular Sequence Data Mutation Patients Polyomavirus Transplants & implants Urine Virology Virus Cultivation Viruses |
| title | Human endothelial cells allow passage of an archetypal BK virus (BKV) strain : a tool for cultivation and functional studies of natural BKV strains |
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