Porcine endogenous retrovirus encodes xenoantigens involved in porcine cellular xenograft rejection by mice
Identification of the antigens that stimulate transplant rejection can help develop graft-specific antirejection strategies. The xenoantigens recognized during rejection of porcine cellular xenografts have not been clearly defined, but it has been assumed that major histocompatibility complex (MHC)...
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| Veröffentlicht in: | Transplantation 2005-06, Vol.79 (12), p.1674-1682 |
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| creator | SIMEONOVIC, Charmaine J ZIOLKOWSKI, Andrew F GIBBS, Adrian J POPP, Sarah K MILBURN, Peter J LYNCH, Celina-Ann HAMILTON, Peter HARRIS, Karla BROWN, Debra J BAIN, Simon A. F WILSON, J. Dennis |
| description | Identification of the antigens that stimulate transplant rejection can help develop graft-specific antirejection strategies. The xenoantigens recognized during rejection of porcine cellular xenografts have not been clearly defined, but it has been assumed that major histocompatibility complex (MHC) xenoantigens are involved.
The role of porcine endogenous retrovirus (PERV) as a source of xenoantigens was examined. The authors used morphometry to compare the kinetics of swine leukocyte antigen (SLA) pig thyroid xenograft rejection in control mice and mice immunized with PERV PK15 cells (porcine kidney epithelial cells), PERV SLA pig peripheral blood lymphocytes (PBL), PERV virions purified from PK15 cells, and PERV or PERV A pseudotypes produced from infected human 293 cells. The tempo of rejection for cellular xenografts of PERV A pseudotype-producing human 293 cells, uninfected human 293 cells, and PK15 cells in PERV-preimmunized and control mice was also compared.
Mice immunized with PK15 cells rejected pig thyroid xenografts significantly faster at day 5 than control mice and mice immunized with pig PBL. This correlated with the amount of PERV RNA and virions produced, but not with the amount of SLA class I MHC expressed by PK15 cells. Immunization of mice with PERV virions purified from porcine PK15 cells and with PERV virions or PERV A pseudotypes produced by human 293 cells also induced accelerated xenograft rejection by 5 days. Accelerated rejection induced by virus pretreatment was CD4 T-cell dependent and restricted to PERV-expressing cellular xenografts of porcine or nonporcine origin.
PERV acts as a significant source of xenoantigens that target porcine cellular xenografts for rejection. |
| doi_str_mv | 10.1097/01.TP.0000164316.55216.07 |
| format | Article |
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The role of porcine endogenous retrovirus (PERV) as a source of xenoantigens was examined. The authors used morphometry to compare the kinetics of swine leukocyte antigen (SLA) pig thyroid xenograft rejection in control mice and mice immunized with PERV PK15 cells (porcine kidney epithelial cells), PERV SLA pig peripheral blood lymphocytes (PBL), PERV virions purified from PK15 cells, and PERV or PERV A pseudotypes produced from infected human 293 cells. The tempo of rejection for cellular xenografts of PERV A pseudotype-producing human 293 cells, uninfected human 293 cells, and PK15 cells in PERV-preimmunized and control mice was also compared.
Mice immunized with PK15 cells rejected pig thyroid xenografts significantly faster at day 5 than control mice and mice immunized with pig PBL. This correlated with the amount of PERV RNA and virions produced, but not with the amount of SLA class I MHC expressed by PK15 cells. Immunization of mice with PERV virions purified from porcine PK15 cells and with PERV virions or PERV A pseudotypes produced by human 293 cells also induced accelerated xenograft rejection by 5 days. Accelerated rejection induced by virus pretreatment was CD4 T-cell dependent and restricted to PERV-expressing cellular xenografts of porcine or nonporcine origin.
PERV acts as a significant source of xenoantigens that target porcine cellular xenografts for rejection.</description><identifier>ISSN: 0041-1337</identifier><identifier>EISSN: 1534-6080</identifier><identifier>DOI: 10.1097/01.TP.0000164316.55216.07</identifier><identifier>PMID: 15973168</identifier><identifier>CODEN: TRPLAU</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott</publisher><subject>Animals ; Antigens, Heterophile - immunology ; Antigens, Viral - immunology ; Base Sequence ; Biological and medical sciences ; Cell Line ; DNA Primers ; Endogenous Retroviruses - genetics ; Endogenous Retroviruses - isolation & purification ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Graft Rejection - pathology ; Graft Rejection - virology ; Humans ; Major Histocompatibility Complex ; Male ; Medical sciences ; Mice ; Mice, Inbred CBA ; Mice, SCID ; Porcine endogenous retrovirus ; Reverse Transcriptase Polymerase Chain Reaction ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Swine ; Swine, Miniature ; Thyroid Gland - transplantation ; Tissue, organ and graft immunology ; Transplantation, Heterologous - pathology ; Virion - genetics ; Virion - isolation & purification</subject><ispartof>Transplantation, 2005-06, Vol.79 (12), p.1674-1682</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-e8be9eb2472fd64a87ddc349ca7a5522fc7afdc2452c47fcbd7d63f055ed781c3</citedby><cites>FETCH-LOGICAL-c427t-e8be9eb2472fd64a87ddc349ca7a5522fc7afdc2452c47fcbd7d63f055ed781c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16945666$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15973168$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SIMEONOVIC, Charmaine J</creatorcontrib><creatorcontrib>ZIOLKOWSKI, Andrew F</creatorcontrib><creatorcontrib>GIBBS, Adrian J</creatorcontrib><creatorcontrib>POPP, Sarah K</creatorcontrib><creatorcontrib>MILBURN, Peter J</creatorcontrib><creatorcontrib>LYNCH, Celina-Ann</creatorcontrib><creatorcontrib>HAMILTON, Peter</creatorcontrib><creatorcontrib>HARRIS, Karla</creatorcontrib><creatorcontrib>BROWN, Debra J</creatorcontrib><creatorcontrib>BAIN, Simon A. F</creatorcontrib><creatorcontrib>WILSON, J. Dennis</creatorcontrib><title>Porcine endogenous retrovirus encodes xenoantigens involved in porcine cellular xenograft rejection by mice</title><title>Transplantation</title><addtitle>Transplantation</addtitle><description>Identification of the antigens that stimulate transplant rejection can help develop graft-specific antirejection strategies. The xenoantigens recognized during rejection of porcine cellular xenografts have not been clearly defined, but it has been assumed that major histocompatibility complex (MHC) xenoantigens are involved.
The role of porcine endogenous retrovirus (PERV) as a source of xenoantigens was examined. The authors used morphometry to compare the kinetics of swine leukocyte antigen (SLA) pig thyroid xenograft rejection in control mice and mice immunized with PERV PK15 cells (porcine kidney epithelial cells), PERV SLA pig peripheral blood lymphocytes (PBL), PERV virions purified from PK15 cells, and PERV or PERV A pseudotypes produced from infected human 293 cells. The tempo of rejection for cellular xenografts of PERV A pseudotype-producing human 293 cells, uninfected human 293 cells, and PK15 cells in PERV-preimmunized and control mice was also compared.
Mice immunized with PK15 cells rejected pig thyroid xenografts significantly faster at day 5 than control mice and mice immunized with pig PBL. This correlated with the amount of PERV RNA and virions produced, but not with the amount of SLA class I MHC expressed by PK15 cells. Immunization of mice with PERV virions purified from porcine PK15 cells and with PERV virions or PERV A pseudotypes produced by human 293 cells also induced accelerated xenograft rejection by 5 days. Accelerated rejection induced by virus pretreatment was CD4 T-cell dependent and restricted to PERV-expressing cellular xenografts of porcine or nonporcine origin.
PERV acts as a significant source of xenoantigens that target porcine cellular xenografts for rejection.</description><subject>Animals</subject><subject>Antigens, Heterophile - immunology</subject><subject>Antigens, Viral - immunology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>DNA Primers</subject><subject>Endogenous Retroviruses - genetics</subject><subject>Endogenous Retroviruses - isolation & purification</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Graft Rejection - pathology</subject><subject>Graft Rejection - virology</subject><subject>Humans</subject><subject>Major Histocompatibility Complex</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred CBA</subject><subject>Mice, SCID</subject><subject>Porcine endogenous retrovirus</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Swine</subject><subject>Swine, Miniature</subject><subject>Thyroid Gland - transplantation</subject><subject>Tissue, organ and graft immunology</subject><subject>Transplantation, Heterologous - pathology</subject><subject>Virion - genetics</subject><subject>Virion - isolation & purification</subject><issn>0041-1337</issn><issn>1534-6080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9PGzEQxS0EKoH2K6DlQG8b_N-7R4Roi4REDunZ8tpjZLqxU3sTwbfHwEo54oM9kn_vzYweQpcELwnu1TUmy_VqieshkjMil0LQemN1hBZEMN5K3OFjtMCYk5Ywpk7RWSnPlRdMqW_olIheVV23QP9WKdsQoYHo0hPEtCtNhimnfci1hGiTg9K81B8Tp1CJ0oS4T-MeXC2a7Sy3MI670eQP8ikbP1WbZ7BTSLEZXptNsPAdnXgzFvgxv-fo76-79e2f9uHx9_3tzUNrOVVTC90APQyUK-qd5KZTzlnGe2uUqYtSb5XxzlIuqOXK28EpJ5nHQoBTHbHsHP389N3m9H8HZdKbUN4HNBHqflqqXlJB6JcgUYIJyfoK9p-gzamUDF5vc9iY_KoJ1u-RaEz0eqUPkeiPSDRWVXsxN9kNG3AH5ZxBBa5mwBRrRp9NtKEcONlzIaVkbxG7l_s</recordid><startdate>20050627</startdate><enddate>20050627</enddate><creator>SIMEONOVIC, Charmaine J</creator><creator>ZIOLKOWSKI, Andrew F</creator><creator>GIBBS, Adrian J</creator><creator>POPP, Sarah K</creator><creator>MILBURN, Peter J</creator><creator>LYNCH, Celina-Ann</creator><creator>HAMILTON, Peter</creator><creator>HARRIS, Karla</creator><creator>BROWN, Debra J</creator><creator>BAIN, Simon A. F</creator><creator>WILSON, J. Dennis</creator><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20050627</creationdate><title>Porcine endogenous retrovirus encodes xenoantigens involved in porcine cellular xenograft rejection by mice</title><author>SIMEONOVIC, Charmaine J ; ZIOLKOWSKI, Andrew F ; GIBBS, Adrian J ; POPP, Sarah K ; MILBURN, Peter J ; LYNCH, Celina-Ann ; HAMILTON, Peter ; HARRIS, Karla ; BROWN, Debra J ; BAIN, Simon A. F ; WILSON, J. Dennis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-e8be9eb2472fd64a87ddc349ca7a5522fc7afdc2452c47fcbd7d63f055ed781c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Antigens, Heterophile - immunology</topic><topic>Antigens, Viral - immunology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>DNA Primers</topic><topic>Endogenous Retroviruses - genetics</topic><topic>Endogenous Retroviruses - isolation & purification</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Graft Rejection - pathology</topic><topic>Graft Rejection - virology</topic><topic>Humans</topic><topic>Major Histocompatibility Complex</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred CBA</topic><topic>Mice, SCID</topic><topic>Porcine endogenous retrovirus</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Swine</topic><topic>Swine, Miniature</topic><topic>Thyroid Gland - transplantation</topic><topic>Tissue, organ and graft immunology</topic><topic>Transplantation, Heterologous - pathology</topic><topic>Virion - genetics</topic><topic>Virion - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SIMEONOVIC, Charmaine J</creatorcontrib><creatorcontrib>ZIOLKOWSKI, Andrew F</creatorcontrib><creatorcontrib>GIBBS, Adrian J</creatorcontrib><creatorcontrib>POPP, Sarah K</creatorcontrib><creatorcontrib>MILBURN, Peter J</creatorcontrib><creatorcontrib>LYNCH, Celina-Ann</creatorcontrib><creatorcontrib>HAMILTON, Peter</creatorcontrib><creatorcontrib>HARRIS, Karla</creatorcontrib><creatorcontrib>BROWN, Debra J</creatorcontrib><creatorcontrib>BAIN, Simon A. F</creatorcontrib><creatorcontrib>WILSON, J. Dennis</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Transplantation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SIMEONOVIC, Charmaine J</au><au>ZIOLKOWSKI, Andrew F</au><au>GIBBS, Adrian J</au><au>POPP, Sarah K</au><au>MILBURN, Peter J</au><au>LYNCH, Celina-Ann</au><au>HAMILTON, Peter</au><au>HARRIS, Karla</au><au>BROWN, Debra J</au><au>BAIN, Simon A. F</au><au>WILSON, J. Dennis</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Porcine endogenous retrovirus encodes xenoantigens involved in porcine cellular xenograft rejection by mice</atitle><jtitle>Transplantation</jtitle><addtitle>Transplantation</addtitle><date>2005-06-27</date><risdate>2005</risdate><volume>79</volume><issue>12</issue><spage>1674</spage><epage>1682</epage><pages>1674-1682</pages><issn>0041-1337</issn><eissn>1534-6080</eissn><coden>TRPLAU</coden><abstract>Identification of the antigens that stimulate transplant rejection can help develop graft-specific antirejection strategies. The xenoantigens recognized during rejection of porcine cellular xenografts have not been clearly defined, but it has been assumed that major histocompatibility complex (MHC) xenoantigens are involved.
The role of porcine endogenous retrovirus (PERV) as a source of xenoantigens was examined. The authors used morphometry to compare the kinetics of swine leukocyte antigen (SLA) pig thyroid xenograft rejection in control mice and mice immunized with PERV PK15 cells (porcine kidney epithelial cells), PERV SLA pig peripheral blood lymphocytes (PBL), PERV virions purified from PK15 cells, and PERV or PERV A pseudotypes produced from infected human 293 cells. The tempo of rejection for cellular xenografts of PERV A pseudotype-producing human 293 cells, uninfected human 293 cells, and PK15 cells in PERV-preimmunized and control mice was also compared.
Mice immunized with PK15 cells rejected pig thyroid xenografts significantly faster at day 5 than control mice and mice immunized with pig PBL. This correlated with the amount of PERV RNA and virions produced, but not with the amount of SLA class I MHC expressed by PK15 cells. Immunization of mice with PERV virions purified from porcine PK15 cells and with PERV virions or PERV A pseudotypes produced by human 293 cells also induced accelerated xenograft rejection by 5 days. Accelerated rejection induced by virus pretreatment was CD4 T-cell dependent and restricted to PERV-expressing cellular xenografts of porcine or nonporcine origin.
PERV acts as a significant source of xenoantigens that target porcine cellular xenografts for rejection.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott</pub><pmid>15973168</pmid><doi>10.1097/01.TP.0000164316.55216.07</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Antigens, Heterophile - immunology Antigens, Viral - immunology Base Sequence Biological and medical sciences Cell Line DNA Primers Endogenous Retroviruses - genetics Endogenous Retroviruses - isolation & purification Fundamental and applied biological sciences. Psychology Fundamental immunology Graft Rejection - pathology Graft Rejection - virology Humans Major Histocompatibility Complex Male Medical sciences Mice Mice, Inbred CBA Mice, SCID Porcine endogenous retrovirus Reverse Transcriptase Polymerase Chain Reaction Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Swine Swine, Miniature Thyroid Gland - transplantation Tissue, organ and graft immunology Transplantation, Heterologous - pathology Virion - genetics Virion - isolation & purification |
| title | Porcine endogenous retrovirus encodes xenoantigens involved in porcine cellular xenograft rejection by mice |
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