FIELD EVALUATION OF THE CHESSBOARD MODIFICATION FOR SEROTYPING OF STREPTOCOCCUS PNEUMONIAE IN A SMALL LABORATORY IN BANGLADESH
The chessboard modification of the quellung method for serotyping Streptococcus pneumoniae (Pneumococcus) was introduced in the Gonoshasthaya Vaccine Research Laboratory, a small laboratory in Bangladesh. We applied initial bench-side training and subsequent continuous surveillance and quality assur...
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| Veröffentlicht in: | The American journal of tropical medicine and hygiene 2006-05, Vol.74 (5), p.863-867 |
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| container_title | The American journal of tropical medicine and hygiene |
| container_volume | 74 |
| creator | HERVA, ELJA GRANAT, SIMO MIA, ZAKARIA OLLGREN, JUKKA PIIRAINEN, LIISA MAKELA, P. HELENA |
| description | The chessboard modification of the quellung method for serotyping Streptococcus pneumoniae (Pneumococcus) was introduced in the Gonoshasthaya Vaccine Research Laboratory, a small laboratory in Bangladesh. We applied initial bench-side training and subsequent continuous surveillance and quality assurance as approaches for good laboratory practice. Results obtained a this laboratory on 1,101 consecutive isolates had satisfactory sensitivity (85.1-100%) and specificity (97.9-99.9%) for serotyping the 10 most common serogroups/types of pneumococci when compared with the results obtained in the Finnish reference laboratory for pneumococcal serotyping at the National Public Health Institute. We conclude that serotyping of pneumococci by the chessboard method can be introduced into a small laboratory by providing basic bacteriologic skills, adequate initial training, and continuous external support. |
| doi_str_mv | 10.4269/ajtmh.2006.74.863 |
| format | Article |
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HELENA</creatorcontrib><title>FIELD EVALUATION OF THE CHESSBOARD MODIFICATION FOR SEROTYPING OF STREPTOCOCCUS PNEUMONIAE IN A SMALL LABORATORY IN BANGLADESH</title><title>The American journal of tropical medicine and hygiene</title><addtitle>Am J Trop Med Hyg</addtitle><description>The chessboard modification of the quellung method for serotyping Streptococcus pneumoniae (Pneumococcus) was introduced in the Gonoshasthaya Vaccine Research Laboratory, a small laboratory in Bangladesh. We applied initial bench-side training and subsequent continuous surveillance and quality assurance as approaches for good laboratory practice. Results obtained a this laboratory on 1,101 consecutive isolates had satisfactory sensitivity (85.1-100%) and specificity (97.9-99.9%) for serotyping the 10 most common serogroups/types of pneumococci when compared with the results obtained in the Finnish reference laboratory for pneumococcal serotyping at the National Public Health Institute. We conclude that serotyping of pneumococci by the chessboard method can be introduced into a small laboratory by providing basic bacteriologic skills, adequate initial training, and continuous external support.</description><subject>Adult</subject><subject>Bangladesh - epidemiology</subject><subject>Benchmarking</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>Diagnostic Tests, Routine - methods</subject><subject>Female</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Laboratories, Hospital - standards</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Nasopharynx - microbiology</subject><subject>Pneumococcal Infections - epidemiology</subject><subject>Pneumococcal Infections - microbiology</subject><subject>Population Surveillance - methods</subject><subject>Predictive Value of Tests</subject><subject>Prospective Studies</subject><subject>Quality Assurance, Health Care</subject><subject>Sensitivity and Specificity</subject><subject>Serotyping - methods</subject><subject>Serotyping - standards</subject><subject>Streptococcus pneumoniae</subject><subject>Streptococcus pneumoniae - classification</subject><subject>Streptococcus pneumoniae - immunology</subject><issn>0002-9637</issn><issn>1476-1645</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcGOmzAURa2qVSed9gO6qbxpd2RswDYsHWISJIIjIJVmZRliGkYwmcGJom767UMmkbLs6knvnXfv4gDwHaOp79LwQT8d-t3URYhOmT8NqPcBTLDPqIOpTz6CCULIdULqsTvwxdonhHDgYvwZ3GFKA0ZDfwL-xYlI51D85umGl4nMoIxhuRQwWoqimEmez-FKzpM4iS7nWOawELksH9dJtjjTRZmLdSkjGUWbAq4zsVnJLOECJhnksFjxNIUpn8mclzJ_PG9nPFukfC6K5VfwqdGdNd-u8x5sYlFGSyeVi7ExdWrf8w6Oi9y6MrTymmpbM4wNo4HLfOMjWlOiQ2JC1LjGoK32QqMDSirUUBTUTeUZjIh3D35dcl-G_evR2IPqW1ubrtPPZn-0irKQYuTS_4I49Aii5AziC1gPe2sH06iXoe318FdhpM521LsddbajmK9GO-PPj2v4serN9vZx1TECP6-AtrXumkE_1629cYwxTIh343btn92pHYyyve66MRar0-k01pH3wjdAnpuP</recordid><startdate>20060501</startdate><enddate>20060501</enddate><creator>HERVA, ELJA</creator><creator>GRANAT, SIMO</creator><creator>MIA, ZAKARIA</creator><creator>OLLGREN, JUKKA</creator><creator>PIIRAINEN, LIISA</creator><creator>MAKELA, P. 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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Adult Bangladesh - epidemiology Benchmarking Biological and medical sciences Child Diagnostic Tests, Routine - methods Female Humans Infectious diseases Laboratories, Hospital - standards Male Medical sciences Nasopharynx - microbiology Pneumococcal Infections - epidemiology Pneumococcal Infections - microbiology Population Surveillance - methods Predictive Value of Tests Prospective Studies Quality Assurance, Health Care Sensitivity and Specificity Serotyping - methods Serotyping - standards Streptococcus pneumoniae Streptococcus pneumoniae - classification Streptococcus pneumoniae - immunology |
| title | FIELD EVALUATION OF THE CHESSBOARD MODIFICATION FOR SEROTYPING OF STREPTOCOCCUS PNEUMONIAE IN A SMALL LABORATORY IN BANGLADESH |
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