Genetic analysis of Cryptosporidium from 2414 humans with diarrhoea in England between 1985 and 2000
Health Protection Agency Centre for Infections, 61 Colindale Avenue, London NW9 5EQ, UK Correspondence J. McLauchlin jim.mclauchlin{at}hpa.org.uk Received 19 July 2005 Accepted 5 February 2006 The characterization of Cryptosporidium using DNA extracted from whole faecal samples collected from 2414 h...
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| creator | Leoni, F Amar, C Nichols, G Pedraza-Diaz, S McLauchlin, J |
| description | Health Protection Agency Centre for Infections, 61 Colindale Avenue, London NW9 5EQ, UK
Correspondence J. McLauchlin jim.mclauchlin{at}hpa.org.uk
Received 19 July 2005
Accepted 5 February 2006
The characterization of Cryptosporidium using DNA extracted from whole faecal samples collected from 2414 humans with diarrhoea in England between 1985 and 2000 where cryptosporidial oocysts were detected using conventional methods is described. Characterization was achieved by PCR/RFLP and DNA sequencing of fragments of the Cryptosporidium oocyst wall protein and the 18S rDNA genes. Cryptosporidium parvum was detected in 56.1 % of cases, Cryptosporidium hominis in 41.7 % and a mixture of C. parvum and C. hominis in 0.9 %. In the remainder of cases, Cryptosporidium meleagridis (0.9 %), Cryptosporidium felis (0.2 %), Cryptosporidium andersoni (0.1 %), Cryptosporidium canis (0.04 %), Cryptosporidium suis (0.04 %) and the Cryptosporidium cervine type (0.04 %) were detected.
Abbreviations: COWP, Cryptosporidium oocyst wall protein gene.
Present address: Istituto Zooprofilattico Sperimentale Umbria e Marche, Sezione di Ancona, via Cupa di Posatora 3, 60100 Ancona, Italy.
Present address: Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Ciudad Universitaria s/n, 28040-Madrid, Spain.
The GenBank/EMBL/DDBJ accession numbers for the sequences described in this study are DQ116568 , DQ116569 , DQ116570 , DQ116571 and DQ116572 . |
| doi_str_mv | 10.1099/jmm.0.46251-0 |
| format | Article |
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Correspondence J. McLauchlin jim.mclauchlin{at}hpa.org.uk
Received 19 July 2005
Accepted 5 February 2006
The characterization of Cryptosporidium using DNA extracted from whole faecal samples collected from 2414 humans with diarrhoea in England between 1985 and 2000 where cryptosporidial oocysts were detected using conventional methods is described. Characterization was achieved by PCR/RFLP and DNA sequencing of fragments of the Cryptosporidium oocyst wall protein and the 18S rDNA genes. Cryptosporidium parvum was detected in 56.1 % of cases, Cryptosporidium hominis in 41.7 % and a mixture of C. parvum and C. hominis in 0.9 %. In the remainder of cases, Cryptosporidium meleagridis (0.9 %), Cryptosporidium felis (0.2 %), Cryptosporidium andersoni (0.1 %), Cryptosporidium canis (0.04 %), Cryptosporidium suis (0.04 %) and the Cryptosporidium cervine type (0.04 %) were detected.
Abbreviations: COWP, Cryptosporidium oocyst wall protein gene.
Present address: Istituto Zooprofilattico Sperimentale Umbria e Marche, Sezione di Ancona, via Cupa di Posatora 3, 60100 Ancona, Italy.
Present address: Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Ciudad Universitaria s/n, 28040-Madrid, Spain.
The GenBank/EMBL/DDBJ accession numbers for the sequences described in this study are DQ116568 , DQ116569 , DQ116570 , DQ116571 and DQ116572 .</description><identifier>ISSN: 0022-2615</identifier><identifier>EISSN: 1473-5644</identifier><identifier>DOI: 10.1099/jmm.0.46251-0</identifier><identifier>PMID: 16687587</identifier><identifier>CODEN: JMMIAV</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Adolescent ; Adult ; Animals ; Biological and medical sciences ; Canis ; Child ; Child, Preschool ; Cryptosporidiosis - epidemiology ; Cryptosporidiosis - parasitology ; Cryptosporidium - classification ; Cryptosporidium - genetics ; Cryptosporidium - isolation & purification ; Cryptosporidium - pathogenicity ; Cryptosporidium andersoni ; Cryptosporidium felis ; Cryptosporidium meleagridis ; Cryptosporidium parvum ; Diarrhea - microbiology ; DNA, Protozoan - genetics ; England - epidemiology ; Feces - parasitology ; Fundamental and applied biological sciences. Psychology ; Genes, Protozoan ; Humans ; Infant ; Infectious diseases ; Medical sciences ; Microbiology ; Molecular Sequence Data ; Protozoan Proteins - genetics ; RNA, Protozoan - genetics ; RNA, Ribosomal, 18S - genetics ; Species Specificity</subject><ispartof>Journal of medical microbiology, 2006-06, Vol.55 (6), p.703-707</ispartof><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3380-131066d3472a2be9e8a68ce2fe48523a313d928c8d2cac95d5fe0756bef0eec43</citedby><cites>FETCH-LOGICAL-c3380-131066d3472a2be9e8a68ce2fe48523a313d928c8d2cac95d5fe0756bef0eec43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,3748,3749,27931,27932</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17893416$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16687587$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Leoni, F</creatorcontrib><creatorcontrib>Amar, C</creatorcontrib><creatorcontrib>Nichols, G</creatorcontrib><creatorcontrib>Pedraza-Diaz, S</creatorcontrib><creatorcontrib>McLauchlin, J</creatorcontrib><title>Genetic analysis of Cryptosporidium from 2414 humans with diarrhoea in England between 1985 and 2000</title><title>Journal of medical microbiology</title><addtitle>J Med Microbiol</addtitle><description>Health Protection Agency Centre for Infections, 61 Colindale Avenue, London NW9 5EQ, UK
Correspondence J. McLauchlin jim.mclauchlin{at}hpa.org.uk
Received 19 July 2005
Accepted 5 February 2006
The characterization of Cryptosporidium using DNA extracted from whole faecal samples collected from 2414 humans with diarrhoea in England between 1985 and 2000 where cryptosporidial oocysts were detected using conventional methods is described. Characterization was achieved by PCR/RFLP and DNA sequencing of fragments of the Cryptosporidium oocyst wall protein and the 18S rDNA genes. Cryptosporidium parvum was detected in 56.1 % of cases, Cryptosporidium hominis in 41.7 % and a mixture of C. parvum and C. hominis in 0.9 %. In the remainder of cases, Cryptosporidium meleagridis (0.9 %), Cryptosporidium felis (0.2 %), Cryptosporidium andersoni (0.1 %), Cryptosporidium canis (0.04 %), Cryptosporidium suis (0.04 %) and the Cryptosporidium cervine type (0.04 %) were detected.
Abbreviations: COWP, Cryptosporidium oocyst wall protein gene.
Present address: Istituto Zooprofilattico Sperimentale Umbria e Marche, Sezione di Ancona, via Cupa di Posatora 3, 60100 Ancona, Italy.
Present address: Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Ciudad Universitaria s/n, 28040-Madrid, Spain.
The GenBank/EMBL/DDBJ accession numbers for the sequences described in this study are DQ116568 , DQ116569 , DQ116570 , DQ116571 and DQ116572 .</description><subject>Adolescent</subject><subject>Adult</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Canis</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Cryptosporidiosis - epidemiology</subject><subject>Cryptosporidiosis - parasitology</subject><subject>Cryptosporidium - classification</subject><subject>Cryptosporidium - genetics</subject><subject>Cryptosporidium - isolation & purification</subject><subject>Cryptosporidium - pathogenicity</subject><subject>Cryptosporidium andersoni</subject><subject>Cryptosporidium felis</subject><subject>Cryptosporidium meleagridis</subject><subject>Cryptosporidium parvum</subject><subject>Diarrhea - microbiology</subject><subject>DNA, Protozoan - genetics</subject><subject>England - epidemiology</subject><subject>Feces - parasitology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Protozoan</subject><subject>Humans</subject><subject>Infant</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Protozoan Proteins - genetics</subject><subject>RNA, Protozoan - genetics</subject><subject>RNA, Ribosomal, 18S - genetics</subject><subject>Species Specificity</subject><issn>0022-2615</issn><issn>1473-5644</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0E2LFDEQBuAgiju7evQquainHivfyVGGdVdY8KLnkE5XT2fpjzHpZph_b48zsEdPBcXDW8VLyAcGWwbOfX0ehi1speaKVfCKbJg0olJaytdkA8B5xTVTN-S2lGcAZoRwb8kN09oaZc2GNA844pwiDWPoTyUVOrV0l0-HeSqHKacmLQNt8zRQLpmk3TKEsdBjmjvapJBzN2GgaaT3474PY0NrnI-II2XOKnpecAB4R960oS_4_jrvyO_v9792j9XTz4cfu29PVRTCQsUEA60bIQ0PvEaHNmgbkbcoreIiCCYax220DY8hOtWoFsEoXWMLiFGKO_L5knvI058Fy-yHVCL262c4LcVr4zRoxv8LmeNGW8ZWWF1gzFMpGVt_yGkI-eQZ-HP_fu3fg__Xv4fVf7wGL_WAzYu-Fr6CT1cQSgx9m8MYU3lxxjohmV7dl4vr0r47pox-j-OQ1jfqNJ2PKuW1NyDEX9NrmhU</recordid><startdate>200606</startdate><enddate>200606</enddate><creator>Leoni, F</creator><creator>Amar, C</creator><creator>Nichols, G</creator><creator>Pedraza-Diaz, S</creator><creator>McLauchlin, J</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200606</creationdate><title>Genetic analysis of Cryptosporidium from 2414 humans with diarrhoea in England between 1985 and 2000</title><author>Leoni, F ; Amar, C ; Nichols, G ; Pedraza-Diaz, S ; McLauchlin, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3380-131066d3472a2be9e8a68ce2fe48523a313d928c8d2cac95d5fe0756bef0eec43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Canis</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Cryptosporidiosis - epidemiology</topic><topic>Cryptosporidiosis - parasitology</topic><topic>Cryptosporidium - classification</topic><topic>Cryptosporidium - genetics</topic><topic>Cryptosporidium - isolation & purification</topic><topic>Cryptosporidium - pathogenicity</topic><topic>Cryptosporidium andersoni</topic><topic>Cryptosporidium felis</topic><topic>Cryptosporidium meleagridis</topic><topic>Cryptosporidium parvum</topic><topic>Diarrhea - microbiology</topic><topic>DNA, Protozoan - genetics</topic><topic>England - epidemiology</topic><topic>Feces - parasitology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Protozoan</topic><topic>Humans</topic><topic>Infant</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Protozoan Proteins - genetics</topic><topic>RNA, Protozoan - genetics</topic><topic>RNA, Ribosomal, 18S - genetics</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Leoni, F</creatorcontrib><creatorcontrib>Amar, C</creatorcontrib><creatorcontrib>Nichols, G</creatorcontrib><creatorcontrib>Pedraza-Diaz, S</creatorcontrib><creatorcontrib>McLauchlin, J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Leoni, F</au><au>Amar, C</au><au>Nichols, G</au><au>Pedraza-Diaz, S</au><au>McLauchlin, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic analysis of Cryptosporidium from 2414 humans with diarrhoea in England between 1985 and 2000</atitle><jtitle>Journal of medical microbiology</jtitle><addtitle>J Med Microbiol</addtitle><date>2006-06</date><risdate>2006</risdate><volume>55</volume><issue>6</issue><spage>703</spage><epage>707</epage><pages>703-707</pages><issn>0022-2615</issn><eissn>1473-5644</eissn><coden>JMMIAV</coden><abstract>Health Protection Agency Centre for Infections, 61 Colindale Avenue, London NW9 5EQ, UK
Correspondence J. McLauchlin jim.mclauchlin{at}hpa.org.uk
Received 19 July 2005
Accepted 5 February 2006
The characterization of Cryptosporidium using DNA extracted from whole faecal samples collected from 2414 humans with diarrhoea in England between 1985 and 2000 where cryptosporidial oocysts were detected using conventional methods is described. Characterization was achieved by PCR/RFLP and DNA sequencing of fragments of the Cryptosporidium oocyst wall protein and the 18S rDNA genes. Cryptosporidium parvum was detected in 56.1 % of cases, Cryptosporidium hominis in 41.7 % and a mixture of C. parvum and C. hominis in 0.9 %. In the remainder of cases, Cryptosporidium meleagridis (0.9 %), Cryptosporidium felis (0.2 %), Cryptosporidium andersoni (0.1 %), Cryptosporidium canis (0.04 %), Cryptosporidium suis (0.04 %) and the Cryptosporidium cervine type (0.04 %) were detected.
Abbreviations: COWP, Cryptosporidium oocyst wall protein gene.
Present address: Istituto Zooprofilattico Sperimentale Umbria e Marche, Sezione di Ancona, via Cupa di Posatora 3, 60100 Ancona, Italy.
Present address: Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Ciudad Universitaria s/n, 28040-Madrid, Spain.
The GenBank/EMBL/DDBJ accession numbers for the sequences described in this study are DQ116568 , DQ116569 , DQ116570 , DQ116571 and DQ116572 .</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>16687587</pmid><doi>10.1099/jmm.0.46251-0</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adolescent Adult Animals Biological and medical sciences Canis Child Child, Preschool Cryptosporidiosis - epidemiology Cryptosporidiosis - parasitology Cryptosporidium - classification Cryptosporidium - genetics Cryptosporidium - isolation & purification Cryptosporidium - pathogenicity Cryptosporidium andersoni Cryptosporidium felis Cryptosporidium meleagridis Cryptosporidium parvum Diarrhea - microbiology DNA, Protozoan - genetics England - epidemiology Feces - parasitology Fundamental and applied biological sciences. Psychology Genes, Protozoan Humans Infant Infectious diseases Medical sciences Microbiology Molecular Sequence Data Protozoan Proteins - genetics RNA, Protozoan - genetics RNA, Ribosomal, 18S - genetics Species Specificity |
| title | Genetic analysis of Cryptosporidium from 2414 humans with diarrhoea in England between 1985 and 2000 |
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