PIN Proteins Perform a Rate-Limiting Function in Cellular Auxin Efflux
Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport-related development, yet the molecular function of PINs remains unknow...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2006-05, Vol.312 (5775), p.914-918 |
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| creator | Petrášek, Jan Mravec, Jozef Bouchard, Rodolphe Blakeslee, Joshua J Abas, Melinda Seifertová, Daniela Wiśniewska, Justyna Tadele, Zerihun Kubeš, Martin Čovanová, Milada Dhonukshe, Pankaj Skůpa, Petr Benková, Eva Perry, Lucie Křeček, Pavel Lee, Ok Ran Fink, Gerald R Geisler, Markus Murphy, Angus S Luschnig, Christian Zažímalová, Eva Friml, Jiří |
| description | Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport-related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux. |
| doi_str_mv | 10.1126/science.1123542 |
| format | Article |
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Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport-related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.1123542</identifier><identifier>PMID: 16601150</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Association for the Advancement of Science</publisher><subject>Arabidopsis ; Arabidopsis - cytology ; Arabidopsis - growth & development ; Arabidopsis - metabolism ; Arabidopsis - physiology ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Arabidopsis thaliana ; ATP-Binding Cassette Transporters - genetics ; ATP-Binding Cassette Transporters - metabolism ; Auxins ; Biological and medical sciences ; Biological Transport ; Botany ; cell culture ; Cell lines ; Cell Membrane - metabolism ; Cells ; Cells, Cultured ; Cellular biology ; Chemical agents ; Cultured cells ; efflux ; Fundamental and applied biological sciences. Psychology ; glycoproteins ; Gravitropism ; HeLa Cells ; Hormones ; Humans ; Indoleacetic Acids - metabolism ; Inhibitor drugs ; Insulin antibodies ; Kinetics ; Membrane Transport Proteins - genetics ; Membrane Transport Proteins - metabolism ; Mutation ; Naphthaleneacetic Acids - metabolism ; Nicotiana ; Nicotiana tabacum ; phosphoglycoproteins ; Phthalimides - pharmacology ; physiological transport ; PIN proteins ; Plant growth ; Plant physiology and development ; Plant Roots - physiology ; Proteins ; Reverse transcriptase polymerase chain reaction ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Seedlings ; tobacco ; Transfection ; Transformation, Genetic ; transporters ; Vegetative apparatus, growth and morphogenesis. Senescence ; Yeasts</subject><ispartof>Science (American Association for the Advancement of Science), 2006-05, Vol.312 (5775), p.914-918</ispartof><rights>Copyright 2006 American Association for the Advancement of Science</rights><rights>2006 INIST-CNRS</rights><rights>Copyright American Association for the Advancement of Science May 12, 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c596t-554fcf8364dac0c79c5a36c14e151a2ff652d2bb2b88c5488822c3c04d0aa27a3</citedby><cites>FETCH-LOGICAL-c596t-554fcf8364dac0c79c5a36c14e151a2ff652d2bb2b88c5488822c3c04d0aa27a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3846207$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3846207$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,2884,2885,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17781539$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16601150$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Petrášek, Jan</creatorcontrib><creatorcontrib>Mravec, Jozef</creatorcontrib><creatorcontrib>Bouchard, Rodolphe</creatorcontrib><creatorcontrib>Blakeslee, Joshua J</creatorcontrib><creatorcontrib>Abas, Melinda</creatorcontrib><creatorcontrib>Seifertová, Daniela</creatorcontrib><creatorcontrib>Wiśniewska, Justyna</creatorcontrib><creatorcontrib>Tadele, Zerihun</creatorcontrib><creatorcontrib>Kubeš, Martin</creatorcontrib><creatorcontrib>Čovanová, Milada</creatorcontrib><creatorcontrib>Dhonukshe, Pankaj</creatorcontrib><creatorcontrib>Skůpa, Petr</creatorcontrib><creatorcontrib>Benková, Eva</creatorcontrib><creatorcontrib>Perry, Lucie</creatorcontrib><creatorcontrib>Křeček, Pavel</creatorcontrib><creatorcontrib>Lee, Ok Ran</creatorcontrib><creatorcontrib>Fink, Gerald R</creatorcontrib><creatorcontrib>Geisler, Markus</creatorcontrib><creatorcontrib>Murphy, Angus S</creatorcontrib><creatorcontrib>Luschnig, Christian</creatorcontrib><creatorcontrib>Zažímalová, Eva</creatorcontrib><creatorcontrib>Friml, Jiří</creatorcontrib><title>PIN Proteins Perform a Rate-Limiting Function in Cellular Auxin Efflux</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport-related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux.</description><subject>Arabidopsis</subject><subject>Arabidopsis - cytology</subject><subject>Arabidopsis - growth & development</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis - physiology</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Arabidopsis thaliana</subject><subject>ATP-Binding Cassette Transporters - genetics</subject><subject>ATP-Binding Cassette Transporters - metabolism</subject><subject>Auxins</subject><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>Botany</subject><subject>cell culture</subject><subject>Cell lines</subject><subject>Cell Membrane - metabolism</subject><subject>Cells</subject><subject>Cells, Cultured</subject><subject>Cellular biology</subject><subject>Chemical agents</subject><subject>Cultured cells</subject><subject>efflux</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>glycoproteins</subject><subject>Gravitropism</subject><subject>HeLa Cells</subject><subject>Hormones</subject><subject>Humans</subject><subject>Indoleacetic Acids - metabolism</subject><subject>Inhibitor drugs</subject><subject>Insulin antibodies</subject><subject>Kinetics</subject><subject>Membrane Transport Proteins - genetics</subject><subject>Membrane Transport Proteins - metabolism</subject><subject>Mutation</subject><subject>Naphthaleneacetic Acids - metabolism</subject><subject>Nicotiana</subject><subject>Nicotiana tabacum</subject><subject>phosphoglycoproteins</subject><subject>Phthalimides - pharmacology</subject><subject>physiological transport</subject><subject>PIN proteins</subject><subject>Plant growth</subject><subject>Plant physiology and development</subject><subject>Plant Roots - physiology</subject><subject>Proteins</subject><subject>Reverse transcriptase polymerase chain reaction</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Seedlings</subject><subject>tobacco</subject><subject>Transfection</subject><subject>Transformation, Genetic</subject><subject>transporters</subject><subject>Vegetative apparatus, growth and morphogenesis. 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| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 2006-05, Vol.312 (5775), p.914-918 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67958665 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; American Association for the Advancement of Science |
| subjects | Arabidopsis Arabidopsis - cytology Arabidopsis - growth & development Arabidopsis - metabolism Arabidopsis - physiology Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis thaliana ATP-Binding Cassette Transporters - genetics ATP-Binding Cassette Transporters - metabolism Auxins Biological and medical sciences Biological Transport Botany cell culture Cell lines Cell Membrane - metabolism Cells Cells, Cultured Cellular biology Chemical agents Cultured cells efflux Fundamental and applied biological sciences. Psychology glycoproteins Gravitropism HeLa Cells Hormones Humans Indoleacetic Acids - metabolism Inhibitor drugs Insulin antibodies Kinetics Membrane Transport Proteins - genetics Membrane Transport Proteins - metabolism Mutation Naphthaleneacetic Acids - metabolism Nicotiana Nicotiana tabacum phosphoglycoproteins Phthalimides - pharmacology physiological transport PIN proteins Plant growth Plant physiology and development Plant Roots - physiology Proteins Reverse transcriptase polymerase chain reaction Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Seedlings tobacco Transfection Transformation, Genetic transporters Vegetative apparatus, growth and morphogenesis. Senescence Yeasts |
| title | PIN Proteins Perform a Rate-Limiting Function in Cellular Auxin Efflux |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T19%3A23%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=PIN%20Proteins%20Perform%20a%20Rate-Limiting%20Function%20in%20Cellular%20Auxin%20Efflux&rft.jtitle=Science%20(American%20Association%20for%20the%20Advancement%20of%20Science)&rft.au=Petr%C3%A1%C5%A1ek,%20Jan&rft.date=2006-05-12&rft.volume=312&rft.issue=5775&rft.spage=914&rft.epage=918&rft.pages=914-918&rft.issn=0036-8075&rft.eissn=1095-9203&rft.coden=SCIEAS&rft_id=info:doi/10.1126/science.1123542&rft_dat=%3Cjstor_proqu%3E3846207%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=213586847&rft_id=info:pmid/16601150&rft_jstor_id=3846207&rfr_iscdi=true |