Developmental regulation and expression of the zebrafish connexin43 gene
We cloned and sequenced the zebrafish (Danio rerio) connexin43 (Cx43α1) gene. The predicted protein sequence shows a high degree of sequence conservation. Transcript analyses revealed multiple transcription start sites and a potential alternative transcript encoding a N‐terminally truncated Cx43α1 p...
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| Veröffentlicht in: | Developmental dynamics 2005-07, Vol.233 (3), p.890-906 |
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| creator | Chatterjee, Bishwanath Chin, Alvin J. Valdimarsson, Gunnar Finis, Carla Sonntag, Jennifer M. Choi, Bo Yon Tao, Liang Balasubramanian, Krithika Bell, Carolyn Krufka, Alison Kozlowski, David J. Johnson, Ross G. Lo, Cecilia W. |
| description | We cloned and sequenced the zebrafish (Danio rerio) connexin43 (Cx43α1) gene. The predicted protein sequence shows a high degree of sequence conservation. Transcript analyses revealed multiple transcription start sites and a potential alternative transcript encoding a N‐terminally truncated Cx43α1 protein. Maternal Cx43α1 transcripts were detected, with zygotic expression initiated before gastrulation. In situ hybridization revealed many Cx43α1 expression domains, including the notochord and brain, heart and vasculature, many resembling patterns seen in mammalian embryos. Of interest, a reporter construct under control of the mouse Cx43α1 promoter was observed to drive green fluorescent protein expression in zebrafish embryos in domains mimicking the native Cx43α1 expression pattern in fish and mice. Sequence comparison between the mouse and zebrafish Cx43α1 promoter sequences showed the conservation of several transcription factor motifs, which otherwise shared little overall sequence homology. The conservation of protein sequence and developmental gene regulation would suggest that Cx43α1 gap junctions are likely to have conserved roles in vertebrate embryonic development. Developmental Dynamics 233:890–906, 2005. © 2005 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/dvdy.20426 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67953907</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>67953907</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4246-a8a0d67d5950f8b4f1e75d460f14da74decf599326ce997b33699c562a308c5a3</originalsourceid><addsrcrecordid>eNqFkEtLw0AURgdRbH1s_AGSlQshdSbzXkqrVii4UcFVmGRu2kgyiZmktv56G1Nwp6v7XTicxUHoguAJwTi6sWu7nUSYReIAjQnWMsREysN-cxUqqtQInXj_jjFWgpFjNCJcac4IH6P5DNZQVHUJrjVF0MCyK0ybVy4wzgawqRvwvn-rLGhXEHxB0pgs96sgrZyDTe4YDZbg4AwdZabwcL6_p-jl_u55Og8XTw-P09tFmLKIidAog62QlmuOM5WwjIDklgmcEWaNZBbSjGtNI5GC1jKhVGidchEZilXKDT1FV4O3bqqPDnwbl7lPoSiMg6rzsZCaU43lv2BEsCCcRjvwegDTpvK-gSyum7w0zTYmOO4Dx33g-CfwDr7cW7ukBPuL7ovuADIAn3kB2z9U8ex19jZIvwGEDoW2</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>21061532</pqid></control><display><type>article</type><title>Developmental regulation and expression of the zebrafish connexin43 gene</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Access via Wiley Online Library</source><source>Wiley Free Content</source><source>Alma/SFX Local Collection</source><creator>Chatterjee, Bishwanath ; Chin, Alvin J. ; Valdimarsson, Gunnar ; Finis, Carla ; Sonntag, Jennifer M. ; Choi, Bo Yon ; Tao, Liang ; Balasubramanian, Krithika ; Bell, Carolyn ; Krufka, Alison ; Kozlowski, David J. ; Johnson, Ross G. ; Lo, Cecilia W.</creator><creatorcontrib>Chatterjee, Bishwanath ; Chin, Alvin J. ; Valdimarsson, Gunnar ; Finis, Carla ; Sonntag, Jennifer M. ; Choi, Bo Yon ; Tao, Liang ; Balasubramanian, Krithika ; Bell, Carolyn ; Krufka, Alison ; Kozlowski, David J. ; Johnson, Ross G. ; Lo, Cecilia W.</creatorcontrib><description>We cloned and sequenced the zebrafish (Danio rerio) connexin43 (Cx43α1) gene. The predicted protein sequence shows a high degree of sequence conservation. Transcript analyses revealed multiple transcription start sites and a potential alternative transcript encoding a N‐terminally truncated Cx43α1 protein. Maternal Cx43α1 transcripts were detected, with zygotic expression initiated before gastrulation. In situ hybridization revealed many Cx43α1 expression domains, including the notochord and brain, heart and vasculature, many resembling patterns seen in mammalian embryos. Of interest, a reporter construct under control of the mouse Cx43α1 promoter was observed to drive green fluorescent protein expression in zebrafish embryos in domains mimicking the native Cx43α1 expression pattern in fish and mice. Sequence comparison between the mouse and zebrafish Cx43α1 promoter sequences showed the conservation of several transcription factor motifs, which otherwise shared little overall sequence homology. The conservation of protein sequence and developmental gene regulation would suggest that Cx43α1 gap junctions are likely to have conserved roles in vertebrate embryonic development. Developmental Dynamics 233:890–906, 2005. © 2005 Wiley‐Liss, Inc.</description><identifier>ISSN: 1058-8388</identifier><identifier>EISSN: 1097-0177</identifier><identifier>DOI: 10.1002/dvdy.20426</identifier><identifier>PMID: 15895415</identifier><language>eng</language><publisher>New York: Wiley‐Liss, Inc</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Cloning, Molecular ; connexin ; Connexin 43 - chemistry ; Connexin 43 - genetics ; Connexin 43 - metabolism ; Conserved Sequence - genetics ; Cx43 Cx32.2 ; Danio rerio ; DNA, Complementary - genetics ; embryo ; Freshwater ; gap junction ; Gene Expression Profiling ; Gene Expression Regulation, Developmental ; Genomics ; Humans ; In Situ Hybridization ; Mice ; Molecular Sequence Data ; mouse ; Phylogeny ; promoter ; Promoter Regions, Genetic - genetics ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Sequence Alignment ; Transcription Initiation Site ; zebrafish ; Zebrafish - embryology ; Zebrafish - genetics ; Zebrafish - metabolism</subject><ispartof>Developmental dynamics, 2005-07, Vol.233 (3), p.890-906</ispartof><rights>Copyright © 2005 Wiley‐Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4246-a8a0d67d5950f8b4f1e75d460f14da74decf599326ce997b33699c562a308c5a3</citedby><cites>FETCH-LOGICAL-c4246-a8a0d67d5950f8b4f1e75d460f14da74decf599326ce997b33699c562a308c5a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fdvdy.20426$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fdvdy.20426$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,1434,27929,27930,45579,45580,46414,46838</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15895415$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chatterjee, Bishwanath</creatorcontrib><creatorcontrib>Chin, Alvin J.</creatorcontrib><creatorcontrib>Valdimarsson, Gunnar</creatorcontrib><creatorcontrib>Finis, Carla</creatorcontrib><creatorcontrib>Sonntag, Jennifer M.</creatorcontrib><creatorcontrib>Choi, Bo Yon</creatorcontrib><creatorcontrib>Tao, Liang</creatorcontrib><creatorcontrib>Balasubramanian, Krithika</creatorcontrib><creatorcontrib>Bell, Carolyn</creatorcontrib><creatorcontrib>Krufka, Alison</creatorcontrib><creatorcontrib>Kozlowski, David J.</creatorcontrib><creatorcontrib>Johnson, Ross G.</creatorcontrib><creatorcontrib>Lo, Cecilia W.</creatorcontrib><title>Developmental regulation and expression of the zebrafish connexin43 gene</title><title>Developmental dynamics</title><addtitle>Dev Dyn</addtitle><description>We cloned and sequenced the zebrafish (Danio rerio) connexin43 (Cx43α1) gene. The predicted protein sequence shows a high degree of sequence conservation. Transcript analyses revealed multiple transcription start sites and a potential alternative transcript encoding a N‐terminally truncated Cx43α1 protein. Maternal Cx43α1 transcripts were detected, with zygotic expression initiated before gastrulation. In situ hybridization revealed many Cx43α1 expression domains, including the notochord and brain, heart and vasculature, many resembling patterns seen in mammalian embryos. Of interest, a reporter construct under control of the mouse Cx43α1 promoter was observed to drive green fluorescent protein expression in zebrafish embryos in domains mimicking the native Cx43α1 expression pattern in fish and mice. Sequence comparison between the mouse and zebrafish Cx43α1 promoter sequences showed the conservation of several transcription factor motifs, which otherwise shared little overall sequence homology. The conservation of protein sequence and developmental gene regulation would suggest that Cx43α1 gap junctions are likely to have conserved roles in vertebrate embryonic development. Developmental Dynamics 233:890–906, 2005. © 2005 Wiley‐Liss, Inc.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Cloning, Molecular</subject><subject>connexin</subject><subject>Connexin 43 - chemistry</subject><subject>Connexin 43 - genetics</subject><subject>Connexin 43 - metabolism</subject><subject>Conserved Sequence - genetics</subject><subject>Cx43 Cx32.2</subject><subject>Danio rerio</subject><subject>DNA, Complementary - genetics</subject><subject>embryo</subject><subject>Freshwater</subject><subject>gap junction</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Genomics</subject><subject>Humans</subject><subject>In Situ Hybridization</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>mouse</subject><subject>Phylogeny</subject><subject>promoter</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Alignment</subject><subject>Transcription Initiation Site</subject><subject>zebrafish</subject><subject>Zebrafish - embryology</subject><subject>Zebrafish - genetics</subject><subject>Zebrafish - metabolism</subject><issn>1058-8388</issn><issn>1097-0177</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLw0AURgdRbH1s_AGSlQshdSbzXkqrVii4UcFVmGRu2kgyiZmktv56G1Nwp6v7XTicxUHoguAJwTi6sWu7nUSYReIAjQnWMsREysN-cxUqqtQInXj_jjFWgpFjNCJcac4IH6P5DNZQVHUJrjVF0MCyK0ybVy4wzgawqRvwvn-rLGhXEHxB0pgs96sgrZyDTe4YDZbg4AwdZabwcL6_p-jl_u55Og8XTw-P09tFmLKIidAog62QlmuOM5WwjIDklgmcEWaNZBbSjGtNI5GC1jKhVGidchEZilXKDT1FV4O3bqqPDnwbl7lPoSiMg6rzsZCaU43lv2BEsCCcRjvwegDTpvK-gSyum7w0zTYmOO4Dx33g-CfwDr7cW7ukBPuL7ovuADIAn3kB2z9U8ex19jZIvwGEDoW2</recordid><startdate>200507</startdate><enddate>200507</enddate><creator>Chatterjee, Bishwanath</creator><creator>Chin, Alvin J.</creator><creator>Valdimarsson, Gunnar</creator><creator>Finis, Carla</creator><creator>Sonntag, Jennifer M.</creator><creator>Choi, Bo Yon</creator><creator>Tao, Liang</creator><creator>Balasubramanian, Krithika</creator><creator>Bell, Carolyn</creator><creator>Krufka, Alison</creator><creator>Kozlowski, David J.</creator><creator>Johnson, Ross G.</creator><creator>Lo, Cecilia W.</creator><general>Wiley‐Liss, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H98</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200507</creationdate><title>Developmental regulation and expression of the zebrafish connexin43 gene</title><author>Chatterjee, Bishwanath ; Chin, Alvin J. ; Valdimarsson, Gunnar ; Finis, Carla ; Sonntag, Jennifer M. ; Choi, Bo Yon ; Tao, Liang ; Balasubramanian, Krithika ; Bell, Carolyn ; Krufka, Alison ; Kozlowski, David J. ; Johnson, Ross G. ; Lo, Cecilia W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4246-a8a0d67d5950f8b4f1e75d460f14da74decf599326ce997b33699c562a308c5a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Cloning, Molecular</topic><topic>connexin</topic><topic>Connexin 43 - chemistry</topic><topic>Connexin 43 - genetics</topic><topic>Connexin 43 - metabolism</topic><topic>Conserved Sequence - genetics</topic><topic>Cx43 Cx32.2</topic><topic>Danio rerio</topic><topic>DNA, Complementary - genetics</topic><topic>embryo</topic><topic>Freshwater</topic><topic>gap junction</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Genomics</topic><topic>Humans</topic><topic>In Situ Hybridization</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>mouse</topic><topic>Phylogeny</topic><topic>promoter</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Alignment</topic><topic>Transcription Initiation Site</topic><topic>zebrafish</topic><topic>Zebrafish - embryology</topic><topic>Zebrafish - genetics</topic><topic>Zebrafish - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chatterjee, Bishwanath</creatorcontrib><creatorcontrib>Chin, Alvin J.</creatorcontrib><creatorcontrib>Valdimarsson, Gunnar</creatorcontrib><creatorcontrib>Finis, Carla</creatorcontrib><creatorcontrib>Sonntag, Jennifer M.</creatorcontrib><creatorcontrib>Choi, Bo Yon</creatorcontrib><creatorcontrib>Tao, Liang</creatorcontrib><creatorcontrib>Balasubramanian, Krithika</creatorcontrib><creatorcontrib>Bell, Carolyn</creatorcontrib><creatorcontrib>Krufka, Alison</creatorcontrib><creatorcontrib>Kozlowski, David J.</creatorcontrib><creatorcontrib>Johnson, Ross G.</creatorcontrib><creatorcontrib>Lo, Cecilia W.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental dynamics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chatterjee, Bishwanath</au><au>Chin, Alvin J.</au><au>Valdimarsson, Gunnar</au><au>Finis, Carla</au><au>Sonntag, Jennifer M.</au><au>Choi, Bo Yon</au><au>Tao, Liang</au><au>Balasubramanian, Krithika</au><au>Bell, Carolyn</au><au>Krufka, Alison</au><au>Kozlowski, David J.</au><au>Johnson, Ross G.</au><au>Lo, Cecilia W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Developmental regulation and expression of the zebrafish connexin43 gene</atitle><jtitle>Developmental dynamics</jtitle><addtitle>Dev Dyn</addtitle><date>2005-07</date><risdate>2005</risdate><volume>233</volume><issue>3</issue><spage>890</spage><epage>906</epage><pages>890-906</pages><issn>1058-8388</issn><eissn>1097-0177</eissn><abstract>We cloned and sequenced the zebrafish (Danio rerio) connexin43 (Cx43α1) gene. The predicted protein sequence shows a high degree of sequence conservation. Transcript analyses revealed multiple transcription start sites and a potential alternative transcript encoding a N‐terminally truncated Cx43α1 protein. Maternal Cx43α1 transcripts were detected, with zygotic expression initiated before gastrulation. In situ hybridization revealed many Cx43α1 expression domains, including the notochord and brain, heart and vasculature, many resembling patterns seen in mammalian embryos. Of interest, a reporter construct under control of the mouse Cx43α1 promoter was observed to drive green fluorescent protein expression in zebrafish embryos in domains mimicking the native Cx43α1 expression pattern in fish and mice. Sequence comparison between the mouse and zebrafish Cx43α1 promoter sequences showed the conservation of several transcription factor motifs, which otherwise shared little overall sequence homology. The conservation of protein sequence and developmental gene regulation would suggest that Cx43α1 gap junctions are likely to have conserved roles in vertebrate embryonic development. Developmental Dynamics 233:890–906, 2005. © 2005 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>Wiley‐Liss, Inc</pub><pmid>15895415</pmid><doi>10.1002/dvdy.20426</doi><tpages>17</tpages></addata></record> |
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| subjects | Amino Acid Sequence Animals Base Sequence Cloning, Molecular connexin Connexin 43 - chemistry Connexin 43 - genetics Connexin 43 - metabolism Conserved Sequence - genetics Cx43 Cx32.2 Danio rerio DNA, Complementary - genetics embryo Freshwater gap junction Gene Expression Profiling Gene Expression Regulation, Developmental Genomics Humans In Situ Hybridization Mice Molecular Sequence Data mouse Phylogeny promoter Promoter Regions, Genetic - genetics RNA, Messenger - genetics RNA, Messenger - metabolism Sequence Alignment Transcription Initiation Site zebrafish Zebrafish - embryology Zebrafish - genetics Zebrafish - metabolism |
| title | Developmental regulation and expression of the zebrafish connexin43 gene |
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