Anomalous mRNA levels of chromatin remodeling genes in swamp buffalo ( Bubalus bubalis) cloned embryos
The swamp buffalo ( Bubalus bubalis) is a multi-purpose animal in agriculture that is challenged by extinction due to low reproductive efficiency. Nuclear transfer (NT) has been used to preserve special breeds of buffalo, as well as to increase the number of animals. However, cloned buffalo embryos...
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| creator | Suteevun, T. Smith, S.L. Muenthaisong, S. Yang, X. Parnpai, R. Tian, X.C. |
| description | The swamp buffalo (
Bubalus bubalis) is a multi-purpose animal in agriculture that is challenged by extinction due to low reproductive efficiency. Nuclear transfer (NT) has been used to preserve special breeds of buffalo, as well as to increase the number of animals. However, cloned buffalo embryos have impaired development, as in other species. To understand the chromatin remodeling activities in cloned embryos and to improve NT technology, we examined the expression profiles of five genes involved in DNA and histone modifications,
DNMT1,
DNMT3A,
DNMT3B,
HAT1 and
HDAC1, in single swamp buffalo metaphase II oocytes, NT and in vitro fertilized (IVF) embryos from the two-cell to the blastocyst stage, by quantitative real time RT-PCR. We observed similar expression dynamics for all genes studied in the NT and IVF embryos: relatively constant levels of expression for all genes were found from the MII oocyte up to the eight-cell stage; the levels of mRNA for
HAT1 and
DNMT3B continued to be stably expressed up to the blastocyst stage; while dramatic increases were seen for
DNMT3A and
HDAC1. Alternatively, the levels of
DNMT1 started to decrease at the eight-cell stage. Despite the similarity in the dynamics of gene expression, dramatic differences in the relative levels of these genes between NT and IVF embryos were observed. The expression levels of all DNA modifying genes were higher in the NT embryos than in the IVF embryos at the eight-cell and blastocyst stages. The genes
HDAC1 and
HAT1 were also expressed significantly higher at the blastocyst stage in the NT embryos. Our results suggested differences in chromatin remodeling between NT and IVF embryos and that lower levels of DNA passive demethylation and higher levels of DNA de novo methylation occurred in the NT embryos. These observations are novel in the species of buffalo, and may be associated with developmental failure of cloned buffalo embryos due to the transcriptional repression effect of most genes studied here. |
| doi_str_mv | 10.1016/j.theriogenology.2005.09.015 |
| format | Article |
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Bubalus bubalis) is a multi-purpose animal in agriculture that is challenged by extinction due to low reproductive efficiency. Nuclear transfer (NT) has been used to preserve special breeds of buffalo, as well as to increase the number of animals. However, cloned buffalo embryos have impaired development, as in other species. To understand the chromatin remodeling activities in cloned embryos and to improve NT technology, we examined the expression profiles of five genes involved in DNA and histone modifications,
DNMT1,
DNMT3A,
DNMT3B,
HAT1 and
HDAC1, in single swamp buffalo metaphase II oocytes, NT and in vitro fertilized (IVF) embryos from the two-cell to the blastocyst stage, by quantitative real time RT-PCR. We observed similar expression dynamics for all genes studied in the NT and IVF embryos: relatively constant levels of expression for all genes were found from the MII oocyte up to the eight-cell stage; the levels of mRNA for
HAT1 and
DNMT3B continued to be stably expressed up to the blastocyst stage; while dramatic increases were seen for
DNMT3A and
HDAC1. Alternatively, the levels of
DNMT1 started to decrease at the eight-cell stage. Despite the similarity in the dynamics of gene expression, dramatic differences in the relative levels of these genes between NT and IVF embryos were observed. The expression levels of all DNA modifying genes were higher in the NT embryos than in the IVF embryos at the eight-cell and blastocyst stages. The genes
HDAC1 and
HAT1 were also expressed significantly higher at the blastocyst stage in the NT embryos. Our results suggested differences in chromatin remodeling between NT and IVF embryos and that lower levels of DNA passive demethylation and higher levels of DNA de novo methylation occurred in the NT embryos. These observations are novel in the species of buffalo, and may be associated with developmental failure of cloned buffalo embryos due to the transcriptional repression effect of most genes studied here.</description><identifier>ISSN: 0093-691X</identifier><identifier>EISSN: 1879-3231</identifier><identifier>DOI: 10.1016/j.theriogenology.2005.09.015</identifier><identifier>PMID: 16256185</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acetyltransferases - genetics ; Animals ; buffaloes ; Buffaloes - embryology ; Buffaloes - genetics ; Chromatin Assembly and Disassembly - genetics ; cloning (animals) ; Cloning, Organism ; DNA Methylation ; DNMT ; Embryo development ; Embryonic Development ; Epigenetic ; epigenetics ; Fertilization in Vitro - veterinary ; gene expression ; Histone acetylases ; Histone Acetyltransferases ; Histone Deacetylases - genetics ; Histones - genetics ; messenger RNA ; Nuclear Transfer Techniques ; nuclear transplantation ; Preimplantation ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - analysis</subject><ispartof>Theriogenology, 2006-06, Vol.65 (9), p.1704-1715</ispartof><rights>2005 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-cad6e4d0872e79c4c2acb28064b22dff92eef6ec0d93901c224676c7882be1483</citedby><cites>FETCH-LOGICAL-c408t-cad6e4d0872e79c4c2acb28064b22dff92eef6ec0d93901c224676c7882be1483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.theriogenology.2005.09.015$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16256185$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Suteevun, T.</creatorcontrib><creatorcontrib>Smith, S.L.</creatorcontrib><creatorcontrib>Muenthaisong, S.</creatorcontrib><creatorcontrib>Yang, X.</creatorcontrib><creatorcontrib>Parnpai, R.</creatorcontrib><creatorcontrib>Tian, X.C.</creatorcontrib><title>Anomalous mRNA levels of chromatin remodeling genes in swamp buffalo ( Bubalus bubalis) cloned embryos</title><title>Theriogenology</title><addtitle>Theriogenology</addtitle><description>The swamp buffalo (
Bubalus bubalis) is a multi-purpose animal in agriculture that is challenged by extinction due to low reproductive efficiency. Nuclear transfer (NT) has been used to preserve special breeds of buffalo, as well as to increase the number of animals. However, cloned buffalo embryos have impaired development, as in other species. To understand the chromatin remodeling activities in cloned embryos and to improve NT technology, we examined the expression profiles of five genes involved in DNA and histone modifications,
DNMT1,
DNMT3A,
DNMT3B,
HAT1 and
HDAC1, in single swamp buffalo metaphase II oocytes, NT and in vitro fertilized (IVF) embryos from the two-cell to the blastocyst stage, by quantitative real time RT-PCR. We observed similar expression dynamics for all genes studied in the NT and IVF embryos: relatively constant levels of expression for all genes were found from the MII oocyte up to the eight-cell stage; the levels of mRNA for
HAT1 and
DNMT3B continued to be stably expressed up to the blastocyst stage; while dramatic increases were seen for
DNMT3A and
HDAC1. Alternatively, the levels of
DNMT1 started to decrease at the eight-cell stage. Despite the similarity in the dynamics of gene expression, dramatic differences in the relative levels of these genes between NT and IVF embryos were observed. The expression levels of all DNA modifying genes were higher in the NT embryos than in the IVF embryos at the eight-cell and blastocyst stages. The genes
HDAC1 and
HAT1 were also expressed significantly higher at the blastocyst stage in the NT embryos. Our results suggested differences in chromatin remodeling between NT and IVF embryos and that lower levels of DNA passive demethylation and higher levels of DNA de novo methylation occurred in the NT embryos. These observations are novel in the species of buffalo, and may be associated with developmental failure of cloned buffalo embryos due to the transcriptional repression effect of most genes studied here.</description><subject>Acetyltransferases - genetics</subject><subject>Animals</subject><subject>buffaloes</subject><subject>Buffaloes - embryology</subject><subject>Buffaloes - genetics</subject><subject>Chromatin Assembly and Disassembly - genetics</subject><subject>cloning (animals)</subject><subject>Cloning, Organism</subject><subject>DNA Methylation</subject><subject>DNMT</subject><subject>Embryo development</subject><subject>Embryonic Development</subject><subject>Epigenetic</subject><subject>epigenetics</subject><subject>Fertilization in Vitro - veterinary</subject><subject>gene expression</subject><subject>Histone acetylases</subject><subject>Histone Acetyltransferases</subject><subject>Histone Deacetylases - genetics</subject><subject>Histones - genetics</subject><subject>messenger RNA</subject><subject>Nuclear Transfer Techniques</subject><subject>nuclear transplantation</subject><subject>Preimplantation</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - analysis</subject><issn>0093-691X</issn><issn>1879-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMGKFDEQhoMo7rj6CpqDiB66raQzSQe8jIurwqKgLngL3enKbIZ0Z0y6V-btzTAD4s1TQdX3VyUfIS8Z1AyYfLur5ztMPm5xiiFuDzUHWNega2DrB2TFWqWrhjfsIVkB6KaSmv28IE9y3gFAIyV7TC6Y5GvJ2vWKuM0Uxy7EJdPx25cNDXiPIdPoqL1LZTL7iSYc44DBT1tajmKmpZd_d-Oe9otzJUxf0_dL34WypD9Wn99QG-KEA8WxT4eYn5JHBcz47Fwvye31hx9Xn6qbrx8_X21uKiugnSvbDRLFAK3iqLQVlne25y1I0XM-OKc5opNoYdCNBmY5F1JJq9qW98hE21ySV6e9-xR_LZhnM_psMYRuwvJHI5UWiilRwHcn0KaYc0Jn9smPXToYBubo2ezMv57N0bMBbYrnEn9-vrP0Iw5_w2exBXhxAlwXTbdNPpvb7xxYAwyUEFwX4vpEFN947zGZbD1OFgef0M5miP7_3vIHd1eiMg</recordid><startdate>20060601</startdate><enddate>20060601</enddate><creator>Suteevun, T.</creator><creator>Smith, S.L.</creator><creator>Muenthaisong, S.</creator><creator>Yang, X.</creator><creator>Parnpai, R.</creator><creator>Tian, X.C.</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060601</creationdate><title>Anomalous mRNA levels of chromatin remodeling genes in swamp buffalo ( Bubalus bubalis) cloned embryos</title><author>Suteevun, T. ; Smith, S.L. ; Muenthaisong, S. ; Yang, X. ; Parnpai, R. ; Tian, X.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-cad6e4d0872e79c4c2acb28064b22dff92eef6ec0d93901c224676c7882be1483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Acetyltransferases - genetics</topic><topic>Animals</topic><topic>buffaloes</topic><topic>Buffaloes - embryology</topic><topic>Buffaloes - genetics</topic><topic>Chromatin Assembly and Disassembly - genetics</topic><topic>cloning (animals)</topic><topic>Cloning, Organism</topic><topic>DNA Methylation</topic><topic>DNMT</topic><topic>Embryo development</topic><topic>Embryonic Development</topic><topic>Epigenetic</topic><topic>epigenetics</topic><topic>Fertilization in Vitro - veterinary</topic><topic>gene expression</topic><topic>Histone acetylases</topic><topic>Histone Acetyltransferases</topic><topic>Histone Deacetylases - genetics</topic><topic>Histones - genetics</topic><topic>messenger RNA</topic><topic>Nuclear Transfer Techniques</topic><topic>nuclear transplantation</topic><topic>Preimplantation</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suteevun, T.</creatorcontrib><creatorcontrib>Smith, S.L.</creatorcontrib><creatorcontrib>Muenthaisong, S.</creatorcontrib><creatorcontrib>Yang, X.</creatorcontrib><creatorcontrib>Parnpai, R.</creatorcontrib><creatorcontrib>Tian, X.C.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Theriogenology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suteevun, T.</au><au>Smith, S.L.</au><au>Muenthaisong, S.</au><au>Yang, X.</au><au>Parnpai, R.</au><au>Tian, X.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anomalous mRNA levels of chromatin remodeling genes in swamp buffalo ( Bubalus bubalis) cloned embryos</atitle><jtitle>Theriogenology</jtitle><addtitle>Theriogenology</addtitle><date>2006-06-01</date><risdate>2006</risdate><volume>65</volume><issue>9</issue><spage>1704</spage><epage>1715</epage><pages>1704-1715</pages><issn>0093-691X</issn><eissn>1879-3231</eissn><abstract>The swamp buffalo (
Bubalus bubalis) is a multi-purpose animal in agriculture that is challenged by extinction due to low reproductive efficiency. Nuclear transfer (NT) has been used to preserve special breeds of buffalo, as well as to increase the number of animals. However, cloned buffalo embryos have impaired development, as in other species. To understand the chromatin remodeling activities in cloned embryos and to improve NT technology, we examined the expression profiles of five genes involved in DNA and histone modifications,
DNMT1,
DNMT3A,
DNMT3B,
HAT1 and
HDAC1, in single swamp buffalo metaphase II oocytes, NT and in vitro fertilized (IVF) embryos from the two-cell to the blastocyst stage, by quantitative real time RT-PCR. We observed similar expression dynamics for all genes studied in the NT and IVF embryos: relatively constant levels of expression for all genes were found from the MII oocyte up to the eight-cell stage; the levels of mRNA for
HAT1 and
DNMT3B continued to be stably expressed up to the blastocyst stage; while dramatic increases were seen for
DNMT3A and
HDAC1. Alternatively, the levels of
DNMT1 started to decrease at the eight-cell stage. Despite the similarity in the dynamics of gene expression, dramatic differences in the relative levels of these genes between NT and IVF embryos were observed. The expression levels of all DNA modifying genes were higher in the NT embryos than in the IVF embryos at the eight-cell and blastocyst stages. The genes
HDAC1 and
HAT1 were also expressed significantly higher at the blastocyst stage in the NT embryos. Our results suggested differences in chromatin remodeling between NT and IVF embryos and that lower levels of DNA passive demethylation and higher levels of DNA de novo methylation occurred in the NT embryos. These observations are novel in the species of buffalo, and may be associated with developmental failure of cloned buffalo embryos due to the transcriptional repression effect of most genes studied here.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16256185</pmid><doi>10.1016/j.theriogenology.2005.09.015</doi><tpages>12</tpages></addata></record> |
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| ispartof | Theriogenology, 2006-06, Vol.65 (9), p.1704-1715 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Acetyltransferases - genetics Animals buffaloes Buffaloes - embryology Buffaloes - genetics Chromatin Assembly and Disassembly - genetics cloning (animals) Cloning, Organism DNA Methylation DNMT Embryo development Embryonic Development Epigenetic epigenetics Fertilization in Vitro - veterinary gene expression Histone acetylases Histone Acetyltransferases Histone Deacetylases - genetics Histones - genetics messenger RNA Nuclear Transfer Techniques nuclear transplantation Preimplantation Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - analysis |
| title | Anomalous mRNA levels of chromatin remodeling genes in swamp buffalo ( Bubalus bubalis) cloned embryos |
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