Direct and indirect effects of leptin on preadipocyte proliferation and differentiation
Department of Foods and Nutrition, University of Georgia, Athens, Georgia Submitted 7 December 2005 ; accepted in final form 17 January 2006 Leptin has been shown to reduce body fat in vivo. Adipocytes express the leptin receptor; therefore, it is realistic to expect a direct effect of leptin on adi...
Gespeichert in:
| Veröffentlicht in: | American journal of physiology. Regulatory, integrative and comparative physiology integrative and comparative physiology, 2006-06, Vol.290 (6), p.R1557-R1564 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | R1564 |
|---|---|
| container_issue | 6 |
| container_start_page | R1557 |
| container_title | American journal of physiology. Regulatory, integrative and comparative physiology |
| container_volume | 290 |
| creator | Wagoner, Blair Hausman, Dorothy B Harris, Ruth B. S |
| description | Department of Foods and Nutrition, University of Georgia, Athens, Georgia
Submitted 7 December 2005
; accepted in final form 17 January 2006
Leptin has been shown to reduce body fat in vivo. Adipocytes express the leptin receptor; therefore, it is realistic to expect a direct effect of leptin on adipocyte growth and metabolism. In vitro studies examining the effect of leptin on adipocyte metabolism require supraphysiological doses of the protein to see a decrease in lipogenesis or stimulation of lipolysis, implying an indirect action of leptin. It also is possible that leptin reduces adipose mass by inhibiting preadipocyte proliferation (increase in cell number) and/or differentiation (lipid filling). Thus we determined direct and indirect effects of leptin on preadipocyte proliferation and differentiation in vitro. We tested the effect of leptin (0500 ng/ml), serum from leptin-infused rats (0.25% by volume), and adipose tissue-conditioned medium from leptin-infused rats (030% by volume) on preadipocyte proliferation and differentiation in a primary culture of cells from male Sprague-Dawley rat adipose tissue. Leptin (50 ng/ml) stimulated proliferation of preadipocytes ( P < 0.05), but 250 and 500 ng leptin/ml inhibited proliferation of both preadipocyte and stromal vascular cell fractions ( P < 0.01), as measured by [ 3 H]thymidine incorporation. Serum from leptin-infused rats inhibited proliferation of the adipose and stromal vascular fractions ( P = 0.01), but adipose tissue-conditioned medium had no effect on proliferation of either cell fraction. None of the treatments changed preadipocyte differentiation as measured by sn -glycerophosphate dehydrogenase activity. These results suggest that leptin could inhibit preadipocyte proliferation by modifying release of a factor from tissue other than adipose tissue.
adipogenesis; cell culture
Address for reprint requests and other correspondence: R. Harris, Dept. of Foods and Nutrition, Dawson Hall, Univ. of Georgia, Athens, GA 30602 (e-mail: harrisrb{at}uga.edu ) |
| doi_str_mv | 10.1152/ajpregu.00860.2005 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_highw</sourceid><recordid>TN_cdi_proquest_miscellaneous_67946301</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>67946301</sourcerecordid><originalsourceid>FETCH-LOGICAL-c457t-73c7588039554b92245fe32855c9d31bb21a022fb97b7c93062f805fc196138a3</originalsourceid><addsrcrecordid>eNp1kMlOwzAQhi0EgrK8AAeUE7eU8ZqYG2KXkJBQEUfLcezWVZqEOBH07XEXlgun0Xj-b8b6EDrFMMaYkws9bzs7HcYAuYAxAeA7aBQHJMVMwi4aARU0FRjLA3QYwhwAGGV0Hx1gwQiDHI_Q243vrOkTXZeJr8tNY52LJSSNSyrb9r5OmjqJt3Tp28YsexubpvLOdrr3cbSCSx-hzta9X78doz2nq2BPtvUIvd7dTq4f0qfn-8frq6fUMJ71aUZNxvMcqOScFZIQxp2lJOfcyJLioiBYAyGukFmRGUlBEJcDdwZLgWmu6RE63-yNP3ofbOjVwgdjq0rXthmCEplkggKOQbIJmq4JobNOtZ1f6G6pMKiVTrXVqdY61UpnhM6224diYctfZOsvBsabwMxPZx_Rnmpny-CbqpkufxYSCUqoF8x5FoHL_4G7oaom9rP_Jv-Aqi0d_QK7QJgl</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>67946301</pqid></control><display><type>article</type><title>Direct and indirect effects of leptin on preadipocyte proliferation and differentiation</title><source>MEDLINE</source><source>American Physiological Society</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Wagoner, Blair ; Hausman, Dorothy B ; Harris, Ruth B. S</creator><creatorcontrib>Wagoner, Blair ; Hausman, Dorothy B ; Harris, Ruth B. S</creatorcontrib><description>Department of Foods and Nutrition, University of Georgia, Athens, Georgia
Submitted 7 December 2005
; accepted in final form 17 January 2006
Leptin has been shown to reduce body fat in vivo. Adipocytes express the leptin receptor; therefore, it is realistic to expect a direct effect of leptin on adipocyte growth and metabolism. In vitro studies examining the effect of leptin on adipocyte metabolism require supraphysiological doses of the protein to see a decrease in lipogenesis or stimulation of lipolysis, implying an indirect action of leptin. It also is possible that leptin reduces adipose mass by inhibiting preadipocyte proliferation (increase in cell number) and/or differentiation (lipid filling). Thus we determined direct and indirect effects of leptin on preadipocyte proliferation and differentiation in vitro. We tested the effect of leptin (0500 ng/ml), serum from leptin-infused rats (0.25% by volume), and adipose tissue-conditioned medium from leptin-infused rats (030% by volume) on preadipocyte proliferation and differentiation in a primary culture of cells from male Sprague-Dawley rat adipose tissue. Leptin (50 ng/ml) stimulated proliferation of preadipocytes ( P < 0.05), but 250 and 500 ng leptin/ml inhibited proliferation of both preadipocyte and stromal vascular cell fractions ( P < 0.01), as measured by [ 3 H]thymidine incorporation. Serum from leptin-infused rats inhibited proliferation of the adipose and stromal vascular fractions ( P = 0.01), but adipose tissue-conditioned medium had no effect on proliferation of either cell fraction. None of the treatments changed preadipocyte differentiation as measured by sn -glycerophosphate dehydrogenase activity. These results suggest that leptin could inhibit preadipocyte proliferation by modifying release of a factor from tissue other than adipose tissue.
adipogenesis; cell culture
Address for reprint requests and other correspondence: R. Harris, Dept. of Foods and Nutrition, Dawson Hall, Univ. of Georgia, Athens, GA 30602 (e-mail: harrisrb{at}uga.edu )</description><identifier>ISSN: 0363-6119</identifier><identifier>EISSN: 1522-1490</identifier><identifier>DOI: 10.1152/ajpregu.00860.2005</identifier><identifier>PMID: 16424081</identifier><language>eng</language><publisher>United States</publisher><subject>Adipocytes - cytology ; Adipocytes - drug effects ; Adipocytes - metabolism ; Adipogenesis - drug effects ; Animals ; Body Composition - drug effects ; Body Weight - drug effects ; Cell Proliferation - drug effects ; Cell Survival - drug effects ; Culture Media, Conditioned - pharmacology ; Dose-Response Relationship, Drug ; Eating - drug effects ; Glucosephosphate Dehydrogenase - metabolism ; Insulin-Like Growth Factor I - pharmacology ; Leptin - blood ; Leptin - pharmacology ; Male ; Rats ; Rats, Sprague-Dawley ; Stromal Cells - cytology ; Stromal Cells - drug effects</subject><ispartof>American journal of physiology. Regulatory, integrative and comparative physiology, 2006-06, Vol.290 (6), p.R1557-R1564</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-73c7588039554b92245fe32855c9d31bb21a022fb97b7c93062f805fc196138a3</citedby><cites>FETCH-LOGICAL-c457t-73c7588039554b92245fe32855c9d31bb21a022fb97b7c93062f805fc196138a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,3028,27907,27908</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16424081$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wagoner, Blair</creatorcontrib><creatorcontrib>Hausman, Dorothy B</creatorcontrib><creatorcontrib>Harris, Ruth B. S</creatorcontrib><title>Direct and indirect effects of leptin on preadipocyte proliferation and differentiation</title><title>American journal of physiology. Regulatory, integrative and comparative physiology</title><addtitle>Am J Physiol Regul Integr Comp Physiol</addtitle><description>Department of Foods and Nutrition, University of Georgia, Athens, Georgia
Submitted 7 December 2005
; accepted in final form 17 January 2006
Leptin has been shown to reduce body fat in vivo. Adipocytes express the leptin receptor; therefore, it is realistic to expect a direct effect of leptin on adipocyte growth and metabolism. In vitro studies examining the effect of leptin on adipocyte metabolism require supraphysiological doses of the protein to see a decrease in lipogenesis or stimulation of lipolysis, implying an indirect action of leptin. It also is possible that leptin reduces adipose mass by inhibiting preadipocyte proliferation (increase in cell number) and/or differentiation (lipid filling). Thus we determined direct and indirect effects of leptin on preadipocyte proliferation and differentiation in vitro. We tested the effect of leptin (0500 ng/ml), serum from leptin-infused rats (0.25% by volume), and adipose tissue-conditioned medium from leptin-infused rats (030% by volume) on preadipocyte proliferation and differentiation in a primary culture of cells from male Sprague-Dawley rat adipose tissue. Leptin (50 ng/ml) stimulated proliferation of preadipocytes ( P < 0.05), but 250 and 500 ng leptin/ml inhibited proliferation of both preadipocyte and stromal vascular cell fractions ( P < 0.01), as measured by [ 3 H]thymidine incorporation. Serum from leptin-infused rats inhibited proliferation of the adipose and stromal vascular fractions ( P = 0.01), but adipose tissue-conditioned medium had no effect on proliferation of either cell fraction. None of the treatments changed preadipocyte differentiation as measured by sn -glycerophosphate dehydrogenase activity. These results suggest that leptin could inhibit preadipocyte proliferation by modifying release of a factor from tissue other than adipose tissue.
adipogenesis; cell culture
Address for reprint requests and other correspondence: R. Harris, Dept. of Foods and Nutrition, Dawson Hall, Univ. of Georgia, Athens, GA 30602 (e-mail: harrisrb{at}uga.edu )</description><subject>Adipocytes - cytology</subject><subject>Adipocytes - drug effects</subject><subject>Adipocytes - metabolism</subject><subject>Adipogenesis - drug effects</subject><subject>Animals</subject><subject>Body Composition - drug effects</subject><subject>Body Weight - drug effects</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Culture Media, Conditioned - pharmacology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Eating - drug effects</subject><subject>Glucosephosphate Dehydrogenase - metabolism</subject><subject>Insulin-Like Growth Factor I - pharmacology</subject><subject>Leptin - blood</subject><subject>Leptin - pharmacology</subject><subject>Male</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Stromal Cells - cytology</subject><subject>Stromal Cells - drug effects</subject><issn>0363-6119</issn><issn>1522-1490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMlOwzAQhi0EgrK8AAeUE7eU8ZqYG2KXkJBQEUfLcezWVZqEOBH07XEXlgun0Xj-b8b6EDrFMMaYkws9bzs7HcYAuYAxAeA7aBQHJMVMwi4aARU0FRjLA3QYwhwAGGV0Hx1gwQiDHI_Q243vrOkTXZeJr8tNY52LJSSNSyrb9r5OmjqJt3Tp28YsexubpvLOdrr3cbSCSx-hzta9X78doz2nq2BPtvUIvd7dTq4f0qfn-8frq6fUMJ71aUZNxvMcqOScFZIQxp2lJOfcyJLioiBYAyGukFmRGUlBEJcDdwZLgWmu6RE63-yNP3ofbOjVwgdjq0rXthmCEplkggKOQbIJmq4JobNOtZ1f6G6pMKiVTrXVqdY61UpnhM6224diYctfZOsvBsabwMxPZx_Rnmpny-CbqpkufxYSCUqoF8x5FoHL_4G7oaom9rP_Jv-Aqi0d_QK7QJgl</recordid><startdate>20060601</startdate><enddate>20060601</enddate><creator>Wagoner, Blair</creator><creator>Hausman, Dorothy B</creator><creator>Harris, Ruth B. S</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060601</creationdate><title>Direct and indirect effects of leptin on preadipocyte proliferation and differentiation</title><author>Wagoner, Blair ; Hausman, Dorothy B ; Harris, Ruth B. S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-73c7588039554b92245fe32855c9d31bb21a022fb97b7c93062f805fc196138a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adipocytes - cytology</topic><topic>Adipocytes - drug effects</topic><topic>Adipocytes - metabolism</topic><topic>Adipogenesis - drug effects</topic><topic>Animals</topic><topic>Body Composition - drug effects</topic><topic>Body Weight - drug effects</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Culture Media, Conditioned - pharmacology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Eating - drug effects</topic><topic>Glucosephosphate Dehydrogenase - metabolism</topic><topic>Insulin-Like Growth Factor I - pharmacology</topic><topic>Leptin - blood</topic><topic>Leptin - pharmacology</topic><topic>Male</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Stromal Cells - cytology</topic><topic>Stromal Cells - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wagoner, Blair</creatorcontrib><creatorcontrib>Hausman, Dorothy B</creatorcontrib><creatorcontrib>Harris, Ruth B. S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of physiology. Regulatory, integrative and comparative physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wagoner, Blair</au><au>Hausman, Dorothy B</au><au>Harris, Ruth B. S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Direct and indirect effects of leptin on preadipocyte proliferation and differentiation</atitle><jtitle>American journal of physiology. Regulatory, integrative and comparative physiology</jtitle><addtitle>Am J Physiol Regul Integr Comp Physiol</addtitle><date>2006-06-01</date><risdate>2006</risdate><volume>290</volume><issue>6</issue><spage>R1557</spage><epage>R1564</epage><pages>R1557-R1564</pages><issn>0363-6119</issn><eissn>1522-1490</eissn><abstract>Department of Foods and Nutrition, University of Georgia, Athens, Georgia
Submitted 7 December 2005
; accepted in final form 17 January 2006
Leptin has been shown to reduce body fat in vivo. Adipocytes express the leptin receptor; therefore, it is realistic to expect a direct effect of leptin on adipocyte growth and metabolism. In vitro studies examining the effect of leptin on adipocyte metabolism require supraphysiological doses of the protein to see a decrease in lipogenesis or stimulation of lipolysis, implying an indirect action of leptin. It also is possible that leptin reduces adipose mass by inhibiting preadipocyte proliferation (increase in cell number) and/or differentiation (lipid filling). Thus we determined direct and indirect effects of leptin on preadipocyte proliferation and differentiation in vitro. We tested the effect of leptin (0500 ng/ml), serum from leptin-infused rats (0.25% by volume), and adipose tissue-conditioned medium from leptin-infused rats (030% by volume) on preadipocyte proliferation and differentiation in a primary culture of cells from male Sprague-Dawley rat adipose tissue. Leptin (50 ng/ml) stimulated proliferation of preadipocytes ( P < 0.05), but 250 and 500 ng leptin/ml inhibited proliferation of both preadipocyte and stromal vascular cell fractions ( P < 0.01), as measured by [ 3 H]thymidine incorporation. Serum from leptin-infused rats inhibited proliferation of the adipose and stromal vascular fractions ( P = 0.01), but adipose tissue-conditioned medium had no effect on proliferation of either cell fraction. None of the treatments changed preadipocyte differentiation as measured by sn -glycerophosphate dehydrogenase activity. These results suggest that leptin could inhibit preadipocyte proliferation by modifying release of a factor from tissue other than adipose tissue.
adipogenesis; cell culture
Address for reprint requests and other correspondence: R. Harris, Dept. of Foods and Nutrition, Dawson Hall, Univ. of Georgia, Athens, GA 30602 (e-mail: harrisrb{at}uga.edu )</abstract><cop>United States</cop><pmid>16424081</pmid><doi>10.1152/ajpregu.00860.2005</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0363-6119 |
| ispartof | American journal of physiology. Regulatory, integrative and comparative physiology, 2006-06, Vol.290 (6), p.R1557-R1564 |
| issn | 0363-6119 1522-1490 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67946301 |
| source | MEDLINE; American Physiological Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Adipocytes - cytology Adipocytes - drug effects Adipocytes - metabolism Adipogenesis - drug effects Animals Body Composition - drug effects Body Weight - drug effects Cell Proliferation - drug effects Cell Survival - drug effects Culture Media, Conditioned - pharmacology Dose-Response Relationship, Drug Eating - drug effects Glucosephosphate Dehydrogenase - metabolism Insulin-Like Growth Factor I - pharmacology Leptin - blood Leptin - pharmacology Male Rats Rats, Sprague-Dawley Stromal Cells - cytology Stromal Cells - drug effects |
| title | Direct and indirect effects of leptin on preadipocyte proliferation and differentiation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T10%3A48%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_highw&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Direct%20and%20indirect%20effects%20of%20leptin%20on%20preadipocyte%20proliferation%20and%20differentiation&rft.jtitle=American%20journal%20of%20physiology.%20Regulatory,%20integrative%20and%20comparative%20physiology&rft.au=Wagoner,%20Blair&rft.date=2006-06-01&rft.volume=290&rft.issue=6&rft.spage=R1557&rft.epage=R1564&rft.pages=R1557-R1564&rft.issn=0363-6119&rft.eissn=1522-1490&rft_id=info:doi/10.1152/ajpregu.00860.2005&rft_dat=%3Cproquest_highw%3E67946301%3C/proquest_highw%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=67946301&rft_id=info:pmid/16424081&rfr_iscdi=true |