Modified expression of Bcl-2 and SOD1 proteins in lymphocytes from sporadic ALS patients
Markers of oxidative stress have been found in spinal cord, cortex, cerebrospinal fluid, and plasma of SALS patients. Mitochondrial and calcium metabolism dysfunction were also found in peripheral lymphocytes from SALS patients. In this study, we demonstrate that lymphocytes from SALS patients are m...
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| Veröffentlicht in: | Neuroscience letters 2006-05, Vol.399 (3), p.186-190 |
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| container_title | Neuroscience letters |
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| creator | Cova, Emanuela Cereda, Cristina Galli, Alberto Curti, Daniela Finotti, Chiara Di Poto, Cristina Corato, Manuel Mazzini, Giuliano Ceroni, Mauro |
| description | Markers of oxidative stress have been found in spinal cord, cortex, cerebrospinal fluid, and plasma of SALS patients. Mitochondrial and calcium metabolism dysfunction were also found in peripheral lymphocytes from SALS patients. In this study, we demonstrate that lymphocytes from SALS patients are more prone to undergo alteration of cell membrane integrity both in basal conditions and following oxidative stress induced by H
2O
2 treatment. The expression of the antioxidant proteins, Bcl-2, SOD1 and catalase in basal conditions, was significantly lower in lymphocytes from SALS patients than in lymphocytes from age and sex matched controls. Exposure to H
2O
2 induced a time-dependent decrease of Bcl-2 and SOD1 in control lymphocytes. Conversely, the levels of these proteins remained unchanged in SALS lymphocytes even after 18
h stress. Catalase expression was not significantly modified by oxidative stress. Our results demonstrate that two factors involved in the genesis and/or progression of the familial form of the disease with SOD1 mutation are altered also in the sporadic form of ALS and suggest that the oxidative stress protection pathway is deregulated in lymphocytes from ALS patients. |
| doi_str_mv | 10.1016/j.neulet.2006.01.057 |
| format | Article |
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2O
2 treatment. The expression of the antioxidant proteins, Bcl-2, SOD1 and catalase in basal conditions, was significantly lower in lymphocytes from SALS patients than in lymphocytes from age and sex matched controls. Exposure to H
2O
2 induced a time-dependent decrease of Bcl-2 and SOD1 in control lymphocytes. Conversely, the levels of these proteins remained unchanged in SALS lymphocytes even after 18
h stress. Catalase expression was not significantly modified by oxidative stress. Our results demonstrate that two factors involved in the genesis and/or progression of the familial form of the disease with SOD1 mutation are altered also in the sporadic form of ALS and suggest that the oxidative stress protection pathway is deregulated in lymphocytes from ALS patients.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/j.neulet.2006.01.057</identifier><identifier>PMID: 16495003</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Amyotrophic lateral sclerosis ; Amyotrophic Lateral Sclerosis - metabolism ; Amyotrophic Lateral Sclerosis - pathology ; Bcl-2 ; Biological and medical sciences ; Blotting, Western - methods ; Case-Control Studies ; Catalase ; Cerebrospinal fluid. Meninges. Spinal cord ; Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases ; Gene Expression Regulation - drug effects ; Gene Expression Regulation - physiology ; Humans ; Hydrogen Peroxide - pharmacology ; Lymphocytes ; Lymphocytes - drug effects ; Lymphocytes - metabolism ; Medical sciences ; Nervous system (semeiology, syndromes) ; Neurology ; Oxidative stress ; Proto-Oncogene Proteins c-bcl-2 - metabolism ; SOD1 ; Superoxide Dismutase - metabolism ; Superoxide Dismutase-1 ; Time Factors</subject><ispartof>Neuroscience letters, 2006-05, Vol.399 (3), p.186-190</ispartof><rights>2006 Elsevier Ireland Ltd</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-7f746c022724ba4e8bfd11da7005d34fb0f24608d521758cc79fbfcc1ec7eb7a3</citedby><cites>FETCH-LOGICAL-c456t-7f746c022724ba4e8bfd11da7005d34fb0f24608d521758cc79fbfcc1ec7eb7a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.neulet.2006.01.057$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17772251$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16495003$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cova, Emanuela</creatorcontrib><creatorcontrib>Cereda, Cristina</creatorcontrib><creatorcontrib>Galli, Alberto</creatorcontrib><creatorcontrib>Curti, Daniela</creatorcontrib><creatorcontrib>Finotti, Chiara</creatorcontrib><creatorcontrib>Di Poto, Cristina</creatorcontrib><creatorcontrib>Corato, Manuel</creatorcontrib><creatorcontrib>Mazzini, Giuliano</creatorcontrib><creatorcontrib>Ceroni, Mauro</creatorcontrib><title>Modified expression of Bcl-2 and SOD1 proteins in lymphocytes from sporadic ALS patients</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>Markers of oxidative stress have been found in spinal cord, cortex, cerebrospinal fluid, and plasma of SALS patients. Mitochondrial and calcium metabolism dysfunction were also found in peripheral lymphocytes from SALS patients. In this study, we demonstrate that lymphocytes from SALS patients are more prone to undergo alteration of cell membrane integrity both in basal conditions and following oxidative stress induced by H
2O
2 treatment. The expression of the antioxidant proteins, Bcl-2, SOD1 and catalase in basal conditions, was significantly lower in lymphocytes from SALS patients than in lymphocytes from age and sex matched controls. Exposure to H
2O
2 induced a time-dependent decrease of Bcl-2 and SOD1 in control lymphocytes. Conversely, the levels of these proteins remained unchanged in SALS lymphocytes even after 18
h stress. Catalase expression was not significantly modified by oxidative stress. Our results demonstrate that two factors involved in the genesis and/or progression of the familial form of the disease with SOD1 mutation are altered also in the sporadic form of ALS and suggest that the oxidative stress protection pathway is deregulated in lymphocytes from ALS patients.</description><subject>Amyotrophic lateral sclerosis</subject><subject>Amyotrophic Lateral Sclerosis - metabolism</subject><subject>Amyotrophic Lateral Sclerosis - pathology</subject><subject>Bcl-2</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western - methods</subject><subject>Case-Control Studies</subject><subject>Catalase</subject><subject>Cerebrospinal fluid. Meninges. Spinal cord</subject><subject>Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Gene Expression Regulation - physiology</subject><subject>Humans</subject><subject>Hydrogen Peroxide - pharmacology</subject><subject>Lymphocytes</subject><subject>Lymphocytes - drug effects</subject><subject>Lymphocytes - metabolism</subject><subject>Medical sciences</subject><subject>Nervous system (semeiology, syndromes)</subject><subject>Neurology</subject><subject>Oxidative stress</subject><subject>Proto-Oncogene Proteins c-bcl-2 - metabolism</subject><subject>SOD1</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Superoxide Dismutase-1</subject><subject>Time Factors</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1r3DAURUVpaSZJ_0Eo2rQ7O0-ybNmbQprmCyZkkQS6E7L0RDTYlit5SuffR2EGsuvqbs69XA4hZwxKBqw535QTbgdcSg7QlMBKqOUHsmKt5IXsJP9IVlCBKKpOwBE5TmkDADWrxWdyxBrR1QDVivy-D9Y7j5bivzliSj5MNDj60wwFp3qy9PHhF6NzDAv6KVE_0WE3zi_B7BZM1MUw0jSHqK039GL9SGe9eJyWdEo-OT0k_HLIE_J8ffV0eVusH27uLi_WhRF1sxTSSdEY4Fxy0WuBbe8sY1bL_NVWwvXguGigtTVnsm6NkZ3rnTEMjcRe6uqEfN_v5ot_tpgWNfpkcBj0hGGbVCM7IdpOZlDsQRNDShGdmqMfddwpBurNqNqovVH1ZlQBU9lorn097G_7Ee176aAwA98OgE5GDy7qyfj0zkkpOa9Z5n7sOcw2_nqMKplsyqD1Ec2ibPD_f_IKsn2WKA</recordid><startdate>20060522</startdate><enddate>20060522</enddate><creator>Cova, Emanuela</creator><creator>Cereda, Cristina</creator><creator>Galli, Alberto</creator><creator>Curti, Daniela</creator><creator>Finotti, Chiara</creator><creator>Di Poto, Cristina</creator><creator>Corato, Manuel</creator><creator>Mazzini, Giuliano</creator><creator>Ceroni, Mauro</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060522</creationdate><title>Modified expression of Bcl-2 and SOD1 proteins in lymphocytes from sporadic ALS patients</title><author>Cova, Emanuela ; Cereda, Cristina ; Galli, Alberto ; Curti, Daniela ; Finotti, Chiara ; Di Poto, Cristina ; Corato, Manuel ; Mazzini, Giuliano ; Ceroni, Mauro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-7f746c022724ba4e8bfd11da7005d34fb0f24608d521758cc79fbfcc1ec7eb7a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Amyotrophic lateral sclerosis</topic><topic>Amyotrophic Lateral Sclerosis - metabolism</topic><topic>Amyotrophic Lateral Sclerosis - pathology</topic><topic>Bcl-2</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western - methods</topic><topic>Case-Control Studies</topic><topic>Catalase</topic><topic>Cerebrospinal fluid. Meninges. Spinal cord</topic><topic>Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Gene Expression Regulation - physiology</topic><topic>Humans</topic><topic>Hydrogen Peroxide - pharmacology</topic><topic>Lymphocytes</topic><topic>Lymphocytes - drug effects</topic><topic>Lymphocytes - metabolism</topic><topic>Medical sciences</topic><topic>Nervous system (semeiology, syndromes)</topic><topic>Neurology</topic><topic>Oxidative stress</topic><topic>Proto-Oncogene Proteins c-bcl-2 - metabolism</topic><topic>SOD1</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Superoxide Dismutase-1</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cova, Emanuela</creatorcontrib><creatorcontrib>Cereda, Cristina</creatorcontrib><creatorcontrib>Galli, Alberto</creatorcontrib><creatorcontrib>Curti, Daniela</creatorcontrib><creatorcontrib>Finotti, Chiara</creatorcontrib><creatorcontrib>Di Poto, Cristina</creatorcontrib><creatorcontrib>Corato, Manuel</creatorcontrib><creatorcontrib>Mazzini, Giuliano</creatorcontrib><creatorcontrib>Ceroni, Mauro</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cova, Emanuela</au><au>Cereda, Cristina</au><au>Galli, Alberto</au><au>Curti, Daniela</au><au>Finotti, Chiara</au><au>Di Poto, Cristina</au><au>Corato, Manuel</au><au>Mazzini, Giuliano</au><au>Ceroni, Mauro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modified expression of Bcl-2 and SOD1 proteins in lymphocytes from sporadic ALS patients</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>2006-05-22</date><risdate>2006</risdate><volume>399</volume><issue>3</issue><spage>186</spage><epage>190</epage><pages>186-190</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>Markers of oxidative stress have been found in spinal cord, cortex, cerebrospinal fluid, and plasma of SALS patients. Mitochondrial and calcium metabolism dysfunction were also found in peripheral lymphocytes from SALS patients. In this study, we demonstrate that lymphocytes from SALS patients are more prone to undergo alteration of cell membrane integrity both in basal conditions and following oxidative stress induced by H
2O
2 treatment. The expression of the antioxidant proteins, Bcl-2, SOD1 and catalase in basal conditions, was significantly lower in lymphocytes from SALS patients than in lymphocytes from age and sex matched controls. Exposure to H
2O
2 induced a time-dependent decrease of Bcl-2 and SOD1 in control lymphocytes. Conversely, the levels of these proteins remained unchanged in SALS lymphocytes even after 18
h stress. Catalase expression was not significantly modified by oxidative stress. Our results demonstrate that two factors involved in the genesis and/or progression of the familial form of the disease with SOD1 mutation are altered also in the sporadic form of ALS and suggest that the oxidative stress protection pathway is deregulated in lymphocytes from ALS patients.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>16495003</pmid><doi>10.1016/j.neulet.2006.01.057</doi><tpages>5</tpages></addata></record> |
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| ispartof | Neuroscience letters, 2006-05, Vol.399 (3), p.186-190 |
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| subjects | Amyotrophic lateral sclerosis Amyotrophic Lateral Sclerosis - metabolism Amyotrophic Lateral Sclerosis - pathology Bcl-2 Biological and medical sciences Blotting, Western - methods Case-Control Studies Catalase Cerebrospinal fluid. Meninges. Spinal cord Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases Gene Expression Regulation - drug effects Gene Expression Regulation - physiology Humans Hydrogen Peroxide - pharmacology Lymphocytes Lymphocytes - drug effects Lymphocytes - metabolism Medical sciences Nervous system (semeiology, syndromes) Neurology Oxidative stress Proto-Oncogene Proteins c-bcl-2 - metabolism SOD1 Superoxide Dismutase - metabolism Superoxide Dismutase-1 Time Factors |
| title | Modified expression of Bcl-2 and SOD1 proteins in lymphocytes from sporadic ALS patients |
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