G-protein-activated phospholipase C-β, new partners for cell polarity proteins Par3 and Par6
Cell polarity and asymmetric cell division are fundamental traits of all living cells and play an essential role in embryonic development, neuronal cell chirality formation, and maintenance of mammalian epithelial cell morphology. Heterotrimeric GTP-binding proteins (G proteins) are involved in dire...
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| Veröffentlicht in: | Oncogene 2005-06, Vol.24 (26), p.4293-4300 |
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| creator | Cai, Yi Stafford, Lewis J Bryan, Brad A Mitchell, Dianne Liu, Mingyao |
| description | Cell polarity and asymmetric cell division are fundamental traits of all living cells and play an essential role in embryonic development, neuronal cell chirality formation, and maintenance of mammalian epithelial cell morphology. Heterotrimeric GTP-binding proteins (G proteins) are involved in directing cell polarity and asymmetric cell division in different organisms. However, the mechanism for G-protein-mediated cell polarity and asymmetric cell division is poorly understood. In this study, we have demonstrated that G-protein-activated phospholipase C-
β
(PLC-
β
) interacts with cell polarity proteins Par3 and Par6 (Par: partition-defective) to form protein complexes and to mediate downstream signal transduction. The interactions between PLC-
β
and Par proteins are direct and require the extreme C-terminal-specific sequence motifs of PLC-
β
and the PDZ (PSD95/Dlg/ZO-1) domains of Par proteins. Binding of Par proteins with PLC-
β
stimulates PLC-
β
enzymatic activity, leading to the hydrolysis of phosphatidylinositol-4,5-bisphosphate, and the production of diacylglycerol and inositol 1,4,5-triphosphate, important mediators in cell polarity and cell asymmetric division processes. Furthermore, we have shown that coexpression of PLC-
β
with Par proteins induces transcriptional activation coupled to intracellular Ca
2+
and the Wnt signaling pathway. Therefore, our data suggest that the interaction of PLC-
β
with cell polarity Par proteins may serve as a nexus to transduce extracellular signals to transcriptional regulation through G-protein-mediated signaling pathway in cell polarity and cell asymmetric division. |
| doi_str_mv | 10.1038/sj.onc.1208593 |
| format | Article |
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β
(PLC-
β
) interacts with cell polarity proteins Par3 and Par6 (Par: partition-defective) to form protein complexes and to mediate downstream signal transduction. The interactions between PLC-
β
and Par proteins are direct and require the extreme C-terminal-specific sequence motifs of PLC-
β
and the PDZ (PSD95/Dlg/ZO-1) domains of Par proteins. Binding of Par proteins with PLC-
β
stimulates PLC-
β
enzymatic activity, leading to the hydrolysis of phosphatidylinositol-4,5-bisphosphate, and the production of diacylglycerol and inositol 1,4,5-triphosphate, important mediators in cell polarity and cell asymmetric division processes. Furthermore, we have shown that coexpression of PLC-
β
with Par proteins induces transcriptional activation coupled to intracellular Ca
2+
and the Wnt signaling pathway. Therefore, our data suggest that the interaction of PLC-
β
with cell polarity Par proteins may serve as a nexus to transduce extracellular signals to transcriptional regulation through G-protein-mediated signaling pathway in cell polarity and cell asymmetric division.</description><identifier>ISSN: 0950-9232</identifier><identifier>EISSN: 1476-5594</identifier><identifier>DOI: 10.1038/sj.onc.1208593</identifier><identifier>PMID: 15782111</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Adaptor Proteins, Signal Transducing ; Apoptosis ; Biological and medical sciences ; Calcium (intracellular) ; Calcium signalling ; Carrier Proteins - biosynthesis ; Carrier Proteins - metabolism ; Cell Biology ; Cell Culture Techniques ; Cell division ; Cell Division - physiology ; Cell physiology ; Cell Polarity - genetics ; Cell Polarity - physiology ; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes ; Chirality ; Cytology ; Diglycerides ; Embryogenesis ; Enzymatic activity ; Epithelial cells ; Fundamental and applied biological sciences. Psychology ; Gene regulation ; GTP-binding protein ; GTP-Binding Proteins - pharmacology ; Human Genetics ; Humans ; Hydrolysis ; Inositol 1,4,5-trisphosphate receptors ; Internal Medicine ; Intracellular signalling ; Isoenzymes - biosynthesis ; Isoenzymes - pharmacology ; Kidney - cytology ; Kinases ; Medicine ; Medicine & Public Health ; Molecular and cellular biology ; Oncology ; original-paper ; Phosphatidylinositol 4,5-diphosphate ; Phospholipase C ; Phospholipase C beta ; Polarity ; Postsynaptic density proteins ; Proteins ; Receptors, Thrombin - biosynthesis ; Receptors, Thrombin - metabolism ; Signal Transduction ; Transcription activation ; Transcription, Genetic ; Transfection ; Type C Phospholipases - biosynthesis ; Type C Phospholipases - pharmacology ; Wnt protein ; Zonula occludens-1 protein</subject><ispartof>Oncogene, 2005-06, Vol.24 (26), p.4293-4300</ispartof><rights>Springer Nature Limited 2005</rights><rights>2005 INIST-CNRS</rights><rights>COPYRIGHT 2005 Nature Publishing Group</rights><rights>Nature Publishing Group 2005.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c529t-27b9b34a4831541ca653f92698cd1baa219f12ce2e11b27a56e75d3a71b59bd63</citedby><cites>FETCH-LOGICAL-c529t-27b9b34a4831541ca653f92698cd1baa219f12ce2e11b27a56e75d3a71b59bd63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/sj.onc.1208593$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/sj.onc.1208593$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16897264$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15782111$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cai, Yi</creatorcontrib><creatorcontrib>Stafford, Lewis J</creatorcontrib><creatorcontrib>Bryan, Brad A</creatorcontrib><creatorcontrib>Mitchell, Dianne</creatorcontrib><creatorcontrib>Liu, Mingyao</creatorcontrib><title>G-protein-activated phospholipase C-β, new partners for cell polarity proteins Par3 and Par6</title><title>Oncogene</title><addtitle>Oncogene</addtitle><addtitle>Oncogene</addtitle><description>Cell polarity and asymmetric cell division are fundamental traits of all living cells and play an essential role in embryonic development, neuronal cell chirality formation, and maintenance of mammalian epithelial cell morphology. Heterotrimeric GTP-binding proteins (G proteins) are involved in directing cell polarity and asymmetric cell division in different organisms. However, the mechanism for G-protein-mediated cell polarity and asymmetric cell division is poorly understood. In this study, we have demonstrated that G-protein-activated phospholipase C-
β
(PLC-
β
) interacts with cell polarity proteins Par3 and Par6 (Par: partition-defective) to form protein complexes and to mediate downstream signal transduction. The interactions between PLC-
β
and Par proteins are direct and require the extreme C-terminal-specific sequence motifs of PLC-
β
and the PDZ (PSD95/Dlg/ZO-1) domains of Par proteins. Binding of Par proteins with PLC-
β
stimulates PLC-
β
enzymatic activity, leading to the hydrolysis of phosphatidylinositol-4,5-bisphosphate, and the production of diacylglycerol and inositol 1,4,5-triphosphate, important mediators in cell polarity and cell asymmetric division processes. Furthermore, we have shown that coexpression of PLC-
β
with Par proteins induces transcriptional activation coupled to intracellular Ca
2+
and the Wnt signaling pathway. Therefore, our data suggest that the interaction of PLC-
β
with cell polarity Par proteins may serve as a nexus to transduce extracellular signals to transcriptional regulation through G-protein-mediated signaling pathway in cell polarity and cell asymmetric division.</description><subject>Adaptor Proteins, Signal Transducing</subject><subject>Apoptosis</subject><subject>Biological and medical sciences</subject><subject>Calcium (intracellular)</subject><subject>Calcium signalling</subject><subject>Carrier Proteins - biosynthesis</subject><subject>Carrier Proteins - metabolism</subject><subject>Cell Biology</subject><subject>Cell Culture Techniques</subject><subject>Cell division</subject><subject>Cell Division - physiology</subject><subject>Cell physiology</subject><subject>Cell Polarity - genetics</subject><subject>Cell Polarity - physiology</subject><subject>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</subject><subject>Chirality</subject><subject>Cytology</subject><subject>Diglycerides</subject><subject>Embryogenesis</subject><subject>Enzymatic activity</subject><subject>Epithelial cells</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene regulation</subject><subject>GTP-binding protein</subject><subject>GTP-Binding Proteins - pharmacology</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>Hydrolysis</subject><subject>Inositol 1,4,5-trisphosphate receptors</subject><subject>Internal Medicine</subject><subject>Intracellular signalling</subject><subject>Isoenzymes - biosynthesis</subject><subject>Isoenzymes - pharmacology</subject><subject>Kidney - cytology</subject><subject>Kinases</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Molecular and cellular biology</subject><subject>Oncology</subject><subject>original-paper</subject><subject>Phosphatidylinositol 4,5-diphosphate</subject><subject>Phospholipase C</subject><subject>Phospholipase C beta</subject><subject>Polarity</subject><subject>Postsynaptic density proteins</subject><subject>Proteins</subject><subject>Receptors, Thrombin - biosynthesis</subject><subject>Receptors, Thrombin - metabolism</subject><subject>Signal Transduction</subject><subject>Transcription activation</subject><subject>Transcription, Genetic</subject><subject>Transfection</subject><subject>Type C Phospholipases - biosynthesis</subject><subject>Type C Phospholipases - pharmacology</subject><subject>Wnt protein</subject><subject>Zonula occludens-1 protein</subject><issn>0950-9232</issn><issn>1476-5594</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkc2KFDEUhYMoTju6dSkB0ZXVk5_K33JodBQGdKFLCbdSqTFNdVIm1cq8lg_iM5liChpkRELIJfnuybkchJ5TsqWE64uy36botpQRLQx_gDa0VbIRwrQP0YYYQRrDODtDT0rZE0KUIewxOqNCaUYp3aCvV82U0-xDbMDN4QfMvsfTt1TqHsMExeNd8_vXGxz9TzxBnqPPBQ8pY-fHEU9phBzmW7yKFPwJMscQ-6WQT9GjAcbin63nOfry7u3n3fvm-uPVh93ldeMEM3PDVGc63kKrORUtdSAFHwyTRruedgCMmoEy55mntGMKhPRK9BwU7YTpesnP0es73Wrj-9GX2R5CWQxC9OlYrFSGG6L1f0GqREuIJhV8-Re4T8cc6xCWyZZywRmRJ-oGRm9DHNKcwS2S9pJqQ6QWcqG291B19f4QXIp-CPX-vgaXUynZD3bK4QD51lJil9ht2dsau11jrw0vVrfH7uD7E77mXIFXKwDFwThkiC6UEye1UXWuyl3ccaU-xRufT2P_4-s_FvDDnA</recordid><startdate>20050616</startdate><enddate>20050616</enddate><creator>Cai, Yi</creator><creator>Stafford, Lewis J</creator><creator>Bryan, Brad A</creator><creator>Mitchell, Dianne</creator><creator>Liu, Mingyao</creator><general>Nature Publishing Group UK</general><general>Nature Publishing</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20050616</creationdate><title>G-protein-activated phospholipase C-β, new partners for cell polarity proteins Par3 and Par6</title><author>Cai, Yi ; Stafford, Lewis J ; Bryan, Brad A ; Mitchell, Dianne ; Liu, Mingyao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c529t-27b9b34a4831541ca653f92698cd1baa219f12ce2e11b27a56e75d3a71b59bd63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adaptor Proteins, Signal Transducing</topic><topic>Apoptosis</topic><topic>Biological and medical sciences</topic><topic>Calcium (intracellular)</topic><topic>Calcium signalling</topic><topic>Carrier Proteins - biosynthesis</topic><topic>Carrier Proteins - metabolism</topic><topic>Cell Biology</topic><topic>Cell Culture Techniques</topic><topic>Cell division</topic><topic>Cell Division - physiology</topic><topic>Cell physiology</topic><topic>Cell Polarity - genetics</topic><topic>Cell Polarity - physiology</topic><topic>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</topic><topic>Chirality</topic><topic>Cytology</topic><topic>Diglycerides</topic><topic>Embryogenesis</topic><topic>Enzymatic activity</topic><topic>Epithelial cells</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene regulation</topic><topic>GTP-binding protein</topic><topic>GTP-Binding Proteins - pharmacology</topic><topic>Human Genetics</topic><topic>Humans</topic><topic>Hydrolysis</topic><topic>Inositol 1,4,5-trisphosphate receptors</topic><topic>Internal Medicine</topic><topic>Intracellular signalling</topic><topic>Isoenzymes - biosynthesis</topic><topic>Isoenzymes - pharmacology</topic><topic>Kidney - cytology</topic><topic>Kinases</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Molecular and cellular biology</topic><topic>Oncology</topic><topic>original-paper</topic><topic>Phosphatidylinositol 4,5-diphosphate</topic><topic>Phospholipase C</topic><topic>Phospholipase C beta</topic><topic>Polarity</topic><topic>Postsynaptic density proteins</topic><topic>Proteins</topic><topic>Receptors, Thrombin - biosynthesis</topic><topic>Receptors, Thrombin - metabolism</topic><topic>Signal Transduction</topic><topic>Transcription activation</topic><topic>Transcription, Genetic</topic><topic>Transfection</topic><topic>Type C Phospholipases - biosynthesis</topic><topic>Type C Phospholipases - pharmacology</topic><topic>Wnt protein</topic><topic>Zonula occludens-1 protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cai, Yi</creatorcontrib><creatorcontrib>Stafford, Lewis J</creatorcontrib><creatorcontrib>Bryan, Brad A</creatorcontrib><creatorcontrib>Mitchell, Dianne</creatorcontrib><creatorcontrib>Liu, Mingyao</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Oncogene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cai, Yi</au><au>Stafford, Lewis J</au><au>Bryan, Brad A</au><au>Mitchell, Dianne</au><au>Liu, Mingyao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>G-protein-activated phospholipase C-β, new partners for cell polarity proteins Par3 and Par6</atitle><jtitle>Oncogene</jtitle><stitle>Oncogene</stitle><addtitle>Oncogene</addtitle><date>2005-06-16</date><risdate>2005</risdate><volume>24</volume><issue>26</issue><spage>4293</spage><epage>4300</epage><pages>4293-4300</pages><issn>0950-9232</issn><eissn>1476-5594</eissn><abstract>Cell polarity and asymmetric cell division are fundamental traits of all living cells and play an essential role in embryonic development, neuronal cell chirality formation, and maintenance of mammalian epithelial cell morphology. Heterotrimeric GTP-binding proteins (G proteins) are involved in directing cell polarity and asymmetric cell division in different organisms. However, the mechanism for G-protein-mediated cell polarity and asymmetric cell division is poorly understood. In this study, we have demonstrated that G-protein-activated phospholipase C-
β
(PLC-
β
) interacts with cell polarity proteins Par3 and Par6 (Par: partition-defective) to form protein complexes and to mediate downstream signal transduction. The interactions between PLC-
β
and Par proteins are direct and require the extreme C-terminal-specific sequence motifs of PLC-
β
and the PDZ (PSD95/Dlg/ZO-1) domains of Par proteins. Binding of Par proteins with PLC-
β
stimulates PLC-
β
enzymatic activity, leading to the hydrolysis of phosphatidylinositol-4,5-bisphosphate, and the production of diacylglycerol and inositol 1,4,5-triphosphate, important mediators in cell polarity and cell asymmetric division processes. Furthermore, we have shown that coexpression of PLC-
β
with Par proteins induces transcriptional activation coupled to intracellular Ca
2+
and the Wnt signaling pathway. Therefore, our data suggest that the interaction of PLC-
β
with cell polarity Par proteins may serve as a nexus to transduce extracellular signals to transcriptional regulation through G-protein-mediated signaling pathway in cell polarity and cell asymmetric division.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>15782111</pmid><doi>10.1038/sj.onc.1208593</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 0950-9232 1476-5594 |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals; Nature Journals Online; EZB-FREE-00999 freely available EZB journals |
| subjects | Adaptor Proteins, Signal Transducing Apoptosis Biological and medical sciences Calcium (intracellular) Calcium signalling Carrier Proteins - biosynthesis Carrier Proteins - metabolism Cell Biology Cell Culture Techniques Cell division Cell Division - physiology Cell physiology Cell Polarity - genetics Cell Polarity - physiology Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes Chirality Cytology Diglycerides Embryogenesis Enzymatic activity Epithelial cells Fundamental and applied biological sciences. Psychology Gene regulation GTP-binding protein GTP-Binding Proteins - pharmacology Human Genetics Humans Hydrolysis Inositol 1,4,5-trisphosphate receptors Internal Medicine Intracellular signalling Isoenzymes - biosynthesis Isoenzymes - pharmacology Kidney - cytology Kinases Medicine Medicine & Public Health Molecular and cellular biology Oncology original-paper Phosphatidylinositol 4,5-diphosphate Phospholipase C Phospholipase C beta Polarity Postsynaptic density proteins Proteins Receptors, Thrombin - biosynthesis Receptors, Thrombin - metabolism Signal Transduction Transcription activation Transcription, Genetic Transfection Type C Phospholipases - biosynthesis Type C Phospholipases - pharmacology Wnt protein Zonula occludens-1 protein |
| title | G-protein-activated phospholipase C-β, new partners for cell polarity proteins Par3 and Par6 |
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