Addition of glycerol for improved methylation linkage analysis of polysaccharides

Addition of glycerol for improved methylation linkage analysis of polysaccharides

A presolubilization procedure with the use of glycerol is shown to be applicable for the structural analysis of polysaccharides. Neutral, acidic, high-molecular-weight and low-molecular-weight polysaccharides were solubilized in glycerol prior to methylation and subsequent linkage analysis by GC–MS....

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Veröffentlicht in:Carbohydrate research 2006-06, Vol.341 (8), p.1061-1064
Hauptverfasser: Kim, John S., Reuhs, Bradley L., Michon, Francis, Kaiser, Raymond E., Arumugham, Rasappa G.
Format: Artikel
Sprache:eng
Schlagworte:
Gas chromatography
Gas Chromatography-Mass Spectrometry
Glycerol - chemistry
Linkage analysis
Methylation
Molecular Weight
Oxidation-Reduction
PMAA
Polysaccharide
Polysaccharides - chemistry
Solubility
Sugar Alcohols - chemistry
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Beschreibung
Zusammenfassung:A presolubilization procedure with the use of glycerol is shown to be applicable for the structural analysis of polysaccharides. Neutral, acidic, high-molecular-weight and low-molecular-weight polysaccharides were solubilized in glycerol prior to methylation and subsequent linkage analysis by GC–MS. All four types of polysaccharides showed significant increases in derivatization following presolubilization as measured by recovery of partially methylated alditol acetates.
ISSN:0008-6215
1873-426X
DOI:10.1016/j.carres.2006.03.008