Systematic immunohistochemical profiling of 378 brain tumors with 37 antibodies using tissue microarray technology
We performed a systematic immunohistochemical study on 378 brain tumors using 37 antibodies and tissue microarray (TMA) technology. The aim of this study was to find new diagnostic biomarkers using antibodies established in our laboratory. Our TMA consisted of a grid of 1.5-mm cores that were extrac...
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| Veröffentlicht in: | Acta neuropathologica 2006-05, Vol.111 (5), p.475-482 |
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| creator | Ikota, Hayato Kinjo, Sawako Yokoo, Hideaki Nakazato, Yoichi |
| description | We performed a systematic immunohistochemical study on 378 brain tumors using 37 antibodies and tissue microarray (TMA) technology. The aim of this study was to find new diagnostic biomarkers using antibodies established in our laboratory. Our TMA consisted of a grid of 1.5-mm cores that were extracted from individual donor blocks. Staining for each antibody was scored using a three-point system. We used hierarchical clustering analysis to interpret these data, which resulted in separation of all the brain tumors into seven groups. Although there were some exceptions, cases with the same histological diagnosis were generally grouped together. We then carried out statistical analyses to find the most useful antibodies for grouping of brain tumors. Ten antibodies [glial fibrillary acidic protein (GFAP), Olig2, vimentin, epithelial membrane antigen (EMA), cytokeratin (AE1/AE3), alpha-internexin, nestin, pinealocytes PP5, aquaporin-4 (AQP4) M13d and AQP4M13e] discriminated between astrocytomas and oligodendroglial tumors. Six antibodies [EMA, AE1/AE3, TUJ1, nestin, neurofilament protein-MH (NF-MH) and perivascular cells GP-1] showed significant differences between high-grade and low-grade gliomas. Our data have revealed new antibodies with potential diagnostic utility (Olig2, PP5, GP-1) and demonstrate that TMA technology is highly useful for evaluating newly established antibodies in brain-tumor research. |
| doi_str_mv | 10.1007/s00401-006-0060-1 |
| format | Article |
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The aim of this study was to find new diagnostic biomarkers using antibodies established in our laboratory. Our TMA consisted of a grid of 1.5-mm cores that were extracted from individual donor blocks. Staining for each antibody was scored using a three-point system. We used hierarchical clustering analysis to interpret these data, which resulted in separation of all the brain tumors into seven groups. Although there were some exceptions, cases with the same histological diagnosis were generally grouped together. We then carried out statistical analyses to find the most useful antibodies for grouping of brain tumors. Ten antibodies [glial fibrillary acidic protein (GFAP), Olig2, vimentin, epithelial membrane antigen (EMA), cytokeratin (AE1/AE3), alpha-internexin, nestin, pinealocytes PP5, aquaporin-4 (AQP4) M13d and AQP4M13e] discriminated between astrocytomas and oligodendroglial tumors. Six antibodies [EMA, AE1/AE3, TUJ1, nestin, neurofilament protein-MH (NF-MH) and perivascular cells GP-1] showed significant differences between high-grade and low-grade gliomas. Our data have revealed new antibodies with potential diagnostic utility (Olig2, PP5, GP-1) and demonstrate that TMA technology is highly useful for evaluating newly established antibodies in brain-tumor research.</description><identifier>ISSN: 0001-6322</identifier><identifier>EISSN: 1432-0533</identifier><identifier>DOI: 10.1007/s00401-006-0060-1</identifier><identifier>PMID: 16598485</identifier><language>eng</language><publisher>Germany: Springer Nature B.V</publisher><subject>Antibodies ; Antibodies - immunology ; Antigens ; Aquaporin 4 - immunology ; Aquaporin 4 - metabolism ; Astrocytoma - diagnosis ; Astrocytoma - immunology ; Astrocytoma - metabolism ; Basic Helix-Loop-Helix Transcription Factors - immunology ; Basic Helix-Loop-Helix Transcription Factors - metabolism ; Biomarkers ; Biomarkers, Tumor - immunology ; Biomarkers, Tumor - metabolism ; Brain cancer ; Brain Neoplasms - diagnosis ; Brain Neoplasms - immunology ; Brain Neoplasms - metabolism ; Brain research ; Cluster Analysis ; Diagnosis, Differential ; Glial Fibrillary Acidic Protein - immunology ; Glial Fibrillary Acidic Protein - metabolism ; Humans ; Immunohistochemistry - methods ; Intermediate Filament Proteins - immunology ; Intermediate Filament Proteins - metabolism ; Mucin-1 - immunology ; Mucin-1 - metabolism ; Nerve Tissue Proteins - immunology ; Nerve Tissue Proteins - metabolism ; Nestin ; Oligodendrocyte Transcription Factor 2 ; Oligodendroglioma - diagnosis ; Oligodendroglioma - immunology ; Oligodendroglioma - metabolism ; Pathology ; Prognosis ; Protein Array Analysis - methods ; Proteins ; Tumors ; Vimentin - immunology ; Vimentin - metabolism</subject><ispartof>Acta neuropathologica, 2006-05, Vol.111 (5), p.475-482</ispartof><rights>Springer-Verlag 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c369t-62a57c450aa0071bbb1b64f0cb71493d1cb55cd7a3b687626ca39e196941a3743</citedby><cites>FETCH-LOGICAL-c369t-62a57c450aa0071bbb1b64f0cb71493d1cb55cd7a3b687626ca39e196941a3743</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16598485$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ikota, Hayato</creatorcontrib><creatorcontrib>Kinjo, Sawako</creatorcontrib><creatorcontrib>Yokoo, Hideaki</creatorcontrib><creatorcontrib>Nakazato, Yoichi</creatorcontrib><title>Systematic immunohistochemical profiling of 378 brain tumors with 37 antibodies using tissue microarray technology</title><title>Acta neuropathologica</title><addtitle>Acta Neuropathol</addtitle><description>We performed a systematic immunohistochemical study on 378 brain tumors using 37 antibodies and tissue microarray (TMA) technology. The aim of this study was to find new diagnostic biomarkers using antibodies established in our laboratory. Our TMA consisted of a grid of 1.5-mm cores that were extracted from individual donor blocks. Staining for each antibody was scored using a three-point system. We used hierarchical clustering analysis to interpret these data, which resulted in separation of all the brain tumors into seven groups. Although there were some exceptions, cases with the same histological diagnosis were generally grouped together. We then carried out statistical analyses to find the most useful antibodies for grouping of brain tumors. Ten antibodies [glial fibrillary acidic protein (GFAP), Olig2, vimentin, epithelial membrane antigen (EMA), cytokeratin (AE1/AE3), alpha-internexin, nestin, pinealocytes PP5, aquaporin-4 (AQP4) M13d and AQP4M13e] discriminated between astrocytomas and oligodendroglial tumors. Six antibodies [EMA, AE1/AE3, TUJ1, nestin, neurofilament protein-MH (NF-MH) and perivascular cells GP-1] showed significant differences between high-grade and low-grade gliomas. Our data have revealed new antibodies with potential diagnostic utility (Olig2, PP5, GP-1) and demonstrate that TMA technology is highly useful for evaluating newly established antibodies in brain-tumor research.</description><subject>Antibodies</subject><subject>Antibodies - immunology</subject><subject>Antigens</subject><subject>Aquaporin 4 - immunology</subject><subject>Aquaporin 4 - metabolism</subject><subject>Astrocytoma - diagnosis</subject><subject>Astrocytoma - immunology</subject><subject>Astrocytoma - metabolism</subject><subject>Basic Helix-Loop-Helix Transcription Factors - immunology</subject><subject>Basic Helix-Loop-Helix Transcription Factors - metabolism</subject><subject>Biomarkers</subject><subject>Biomarkers, Tumor - immunology</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Brain cancer</subject><subject>Brain Neoplasms - diagnosis</subject><subject>Brain Neoplasms - immunology</subject><subject>Brain Neoplasms - metabolism</subject><subject>Brain research</subject><subject>Cluster Analysis</subject><subject>Diagnosis, Differential</subject><subject>Glial Fibrillary Acidic Protein - immunology</subject><subject>Glial Fibrillary Acidic Protein - metabolism</subject><subject>Humans</subject><subject>Immunohistochemistry - methods</subject><subject>Intermediate Filament Proteins - immunology</subject><subject>Intermediate Filament Proteins - metabolism</subject><subject>Mucin-1 - immunology</subject><subject>Mucin-1 - metabolism</subject><subject>Nerve Tissue Proteins - immunology</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Nestin</subject><subject>Oligodendrocyte Transcription Factor 2</subject><subject>Oligodendroglioma - diagnosis</subject><subject>Oligodendroglioma - immunology</subject><subject>Oligodendroglioma - metabolism</subject><subject>Pathology</subject><subject>Prognosis</subject><subject>Protein Array Analysis - methods</subject><subject>Proteins</subject><subject>Tumors</subject><subject>Vimentin - immunology</subject><subject>Vimentin - metabolism</subject><issn>0001-6322</issn><issn>1432-0533</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkU9LxDAQxYMo7rr6AbxI8OCtmmnSpD3K4j9Y8KCeS5Km2yxtsyYpst_eLLsgeBhCJr95zMtD6BrIPRAiHgIhjEBGCN8XyeAEzYHRPCMFpadoTkh65TTPZ-gihE265YIV52gGvKhKVhZz5D92IZpBRquxHYZpdJ0N0enODFbLHm-9a21vxzV2LaaixMpLO-I4Dc4H_GNjl7pYjtEq11gT8BT2cLQhTAYnDe-k93KHo9Hd6Hq33l2is1b2wVwdzwX6en76XL5mq_eXt-XjKtOUVzHjuSyEZgWRMnkFpRQozlqilQBW0Qa0KgrdCEkVLwXPuZa0MlDxioGkgtEFujvoJg_fkwmxHmzQpu_laNwUai4qSjmFBN7-Azdu8mParc4BSg6spAmCA5QcheBNW2-9HaTf1UDqfRr1IY06JbGv1EwzN0fhSQ2m-Zs4fj_9BXemhfI</recordid><startdate>200605</startdate><enddate>200605</enddate><creator>Ikota, Hayato</creator><creator>Kinjo, Sawako</creator><creator>Yokoo, Hideaki</creator><creator>Nakazato, Yoichi</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>200605</creationdate><title>Systematic immunohistochemical profiling of 378 brain tumors with 37 antibodies using tissue microarray technology</title><author>Ikota, Hayato ; Kinjo, Sawako ; Yokoo, Hideaki ; Nakazato, Yoichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c369t-62a57c450aa0071bbb1b64f0cb71493d1cb55cd7a3b687626ca39e196941a3743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Antibodies</topic><topic>Antibodies - immunology</topic><topic>Antigens</topic><topic>Aquaporin 4 - immunology</topic><topic>Aquaporin 4 - metabolism</topic><topic>Astrocytoma - diagnosis</topic><topic>Astrocytoma - immunology</topic><topic>Astrocytoma - metabolism</topic><topic>Basic Helix-Loop-Helix Transcription Factors - immunology</topic><topic>Basic Helix-Loop-Helix Transcription Factors - metabolism</topic><topic>Biomarkers</topic><topic>Biomarkers, Tumor - immunology</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Brain cancer</topic><topic>Brain Neoplasms - diagnosis</topic><topic>Brain Neoplasms - immunology</topic><topic>Brain Neoplasms - metabolism</topic><topic>Brain research</topic><topic>Cluster Analysis</topic><topic>Diagnosis, Differential</topic><topic>Glial Fibrillary Acidic Protein - immunology</topic><topic>Glial Fibrillary Acidic Protein - metabolism</topic><topic>Humans</topic><topic>Immunohistochemistry - methods</topic><topic>Intermediate Filament Proteins - immunology</topic><topic>Intermediate Filament Proteins - metabolism</topic><topic>Mucin-1 - immunology</topic><topic>Mucin-1 - metabolism</topic><topic>Nerve Tissue Proteins - immunology</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Nestin</topic><topic>Oligodendrocyte Transcription Factor 2</topic><topic>Oligodendroglioma - diagnosis</topic><topic>Oligodendroglioma - immunology</topic><topic>Oligodendroglioma - metabolism</topic><topic>Pathology</topic><topic>Prognosis</topic><topic>Protein Array Analysis - methods</topic><topic>Proteins</topic><topic>Tumors</topic><topic>Vimentin - immunology</topic><topic>Vimentin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ikota, Hayato</creatorcontrib><creatorcontrib>Kinjo, Sawako</creatorcontrib><creatorcontrib>Yokoo, Hideaki</creatorcontrib><creatorcontrib>Nakazato, Yoichi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Psychology Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Acta neuropathologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ikota, Hayato</au><au>Kinjo, Sawako</au><au>Yokoo, Hideaki</au><au>Nakazato, Yoichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Systematic immunohistochemical profiling of 378 brain tumors with 37 antibodies using tissue microarray technology</atitle><jtitle>Acta neuropathologica</jtitle><addtitle>Acta Neuropathol</addtitle><date>2006-05</date><risdate>2006</risdate><volume>111</volume><issue>5</issue><spage>475</spage><epage>482</epage><pages>475-482</pages><issn>0001-6322</issn><eissn>1432-0533</eissn><abstract>We performed a systematic immunohistochemical study on 378 brain tumors using 37 antibodies and tissue microarray (TMA) technology. The aim of this study was to find new diagnostic biomarkers using antibodies established in our laboratory. Our TMA consisted of a grid of 1.5-mm cores that were extracted from individual donor blocks. Staining for each antibody was scored using a three-point system. We used hierarchical clustering analysis to interpret these data, which resulted in separation of all the brain tumors into seven groups. Although there were some exceptions, cases with the same histological diagnosis were generally grouped together. We then carried out statistical analyses to find the most useful antibodies for grouping of brain tumors. Ten antibodies [glial fibrillary acidic protein (GFAP), Olig2, vimentin, epithelial membrane antigen (EMA), cytokeratin (AE1/AE3), alpha-internexin, nestin, pinealocytes PP5, aquaporin-4 (AQP4) M13d and AQP4M13e] discriminated between astrocytomas and oligodendroglial tumors. Six antibodies [EMA, AE1/AE3, TUJ1, nestin, neurofilament protein-MH (NF-MH) and perivascular cells GP-1] showed significant differences between high-grade and low-grade gliomas. Our data have revealed new antibodies with potential diagnostic utility (Olig2, PP5, GP-1) and demonstrate that TMA technology is highly useful for evaluating newly established antibodies in brain-tumor research.</abstract><cop>Germany</cop><pub>Springer Nature B.V</pub><pmid>16598485</pmid><doi>10.1007/s00401-006-0060-1</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Antibodies Antibodies - immunology Antigens Aquaporin 4 - immunology Aquaporin 4 - metabolism Astrocytoma - diagnosis Astrocytoma - immunology Astrocytoma - metabolism Basic Helix-Loop-Helix Transcription Factors - immunology Basic Helix-Loop-Helix Transcription Factors - metabolism Biomarkers Biomarkers, Tumor - immunology Biomarkers, Tumor - metabolism Brain cancer Brain Neoplasms - diagnosis Brain Neoplasms - immunology Brain Neoplasms - metabolism Brain research Cluster Analysis Diagnosis, Differential Glial Fibrillary Acidic Protein - immunology Glial Fibrillary Acidic Protein - metabolism Humans Immunohistochemistry - methods Intermediate Filament Proteins - immunology Intermediate Filament Proteins - metabolism Mucin-1 - immunology Mucin-1 - metabolism Nerve Tissue Proteins - immunology Nerve Tissue Proteins - metabolism Nestin Oligodendrocyte Transcription Factor 2 Oligodendroglioma - diagnosis Oligodendroglioma - immunology Oligodendroglioma - metabolism Pathology Prognosis Protein Array Analysis - methods Proteins Tumors Vimentin - immunology Vimentin - metabolism |
| title | Systematic immunohistochemical profiling of 378 brain tumors with 37 antibodies using tissue microarray technology |
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