Development of a sensitive, colorometric microarray assay for allergen-responsive human IgE
With the existence of several thousand unique human allergens, a multiplex format, such as protein microarrays, is an attractive option for allergy screening. To determine the feasibility and sensitivity of using an enzyme-based, colorimetric protein microarray assay, three common allergens (mold, d...
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| Veröffentlicht in: | Journal of immunological methods 2005-05, Vol.300 (1), p.24-31 |
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| creator | Lebrun, Stewart J. Petchpud, Wasinee N. Hui, Agnas McLaughlin, Calvin S. |
| description | With the existence of several thousand unique human allergens, a multiplex format, such as protein microarrays, is an attractive option for allergy screening. To determine the feasibility and sensitivity of using an enzyme-based, colorimetric protein microarray assay, three common allergens (mold, dustmite, grass) were arrayed and added sera assayed for responsive human IgE. Normal, low positive, and negative control samples were assayed to determine optimal reaction parameters. Sensitivity of the assay (in international units, IU) was determined by constructing a standard curve using World Health Organization (WHO) standards. The system described here can reliably detect allergen-specific IgE below 0.35 IU, the current WHO standard cutoff. By taking advantage of the sensitivity of enzyme-linked immunosorbent assays (ELISAs) and the multiplex format of microarrays, we have achieved a high throughput system, capable of screening patients for allergen-susceptibility with optimal sensitivity. |
| doi_str_mv | 10.1016/j.jim.2005.01.019 |
| format | Article |
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To determine the feasibility and sensitivity of using an enzyme-based, colorimetric protein microarray assay, three common allergens (mold, dustmite, grass) were arrayed and added sera assayed for responsive human IgE. Normal, low positive, and negative control samples were assayed to determine optimal reaction parameters. Sensitivity of the assay (in international units, IU) was determined by constructing a standard curve using World Health Organization (WHO) standards. The system described here can reliably detect allergen-specific IgE below 0.35 IU, the current WHO standard cutoff. By taking advantage of the sensitivity of enzyme-linked immunosorbent assays (ELISAs) and the multiplex format of microarrays, we have achieved a high throughput system, capable of screening patients for allergen-susceptibility with optimal sensitivity.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/j.jim.2005.01.019</identifier><identifier>PMID: 15893759</identifier><identifier>CODEN: JIMMBG</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Allergens - administration & dosage ; Allergy testing ; Animals ; Biological and medical sciences ; Case-Control Studies ; Colorimetry ; Dust - immunology ; Enzyme-Linked Immunosorbent Assay - methods ; Enzyme-Linked Immunosorbent Assay - statistics & numerical data ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Fungi - immunology ; Humans ; Hypersensitivity, Immediate - diagnosis ; Hypersensitivity, Immediate - immunology ; IgE in vitro test ; Immunoglobulin E - analysis ; Immunoglobulin E - blood ; In Vitro Techniques ; Mites - immunology ; Molecular immunology ; Phleum - immunology ; Protein Array Analysis - methods ; Protein Array Analysis - standards ; Protein Array Analysis - statistics & numerical data ; Protein microarray ; Rhinitis, Allergic, Seasonal - diagnosis ; Rhinitis, Allergic, Seasonal - immunology ; Sensitivity and Specificity ; Techniques</subject><ispartof>Journal of immunological methods, 2005-05, Vol.300 (1), p.24-31</ispartof><rights>2005 Elsevier B.V.</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c509t-1188f4ff846ce8b339569826a02dc41f3cf6701a19e8d7d2a9b4304bd15aa12e3</citedby><cites>FETCH-LOGICAL-c509t-1188f4ff846ce8b339569826a02dc41f3cf6701a19e8d7d2a9b4304bd15aa12e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0022175905000499$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16884266$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15893759$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lebrun, Stewart J.</creatorcontrib><creatorcontrib>Petchpud, Wasinee N.</creatorcontrib><creatorcontrib>Hui, Agnas</creatorcontrib><creatorcontrib>McLaughlin, Calvin S.</creatorcontrib><title>Development of a sensitive, colorometric microarray assay for allergen-responsive human IgE</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>With the existence of several thousand unique human allergens, a multiplex format, such as protein microarrays, is an attractive option for allergy screening. To determine the feasibility and sensitivity of using an enzyme-based, colorimetric protein microarray assay, three common allergens (mold, dustmite, grass) were arrayed and added sera assayed for responsive human IgE. Normal, low positive, and negative control samples were assayed to determine optimal reaction parameters. Sensitivity of the assay (in international units, IU) was determined by constructing a standard curve using World Health Organization (WHO) standards. The system described here can reliably detect allergen-specific IgE below 0.35 IU, the current WHO standard cutoff. By taking advantage of the sensitivity of enzyme-linked immunosorbent assays (ELISAs) and the multiplex format of microarrays, we have achieved a high throughput system, capable of screening patients for allergen-susceptibility with optimal sensitivity.</description><subject>Allergens - administration & dosage</subject><subject>Allergy testing</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Case-Control Studies</subject><subject>Colorimetry</subject><subject>Dust - immunology</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Enzyme-Linked Immunosorbent Assay - statistics & numerical data</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Fungi - immunology</subject><subject>Humans</subject><subject>Hypersensitivity, Immediate - diagnosis</subject><subject>Hypersensitivity, Immediate - immunology</subject><subject>IgE in vitro test</subject><subject>Immunoglobulin E - analysis</subject><subject>Immunoglobulin E - blood</subject><subject>In Vitro Techniques</subject><subject>Mites - immunology</subject><subject>Molecular immunology</subject><subject>Phleum - immunology</subject><subject>Protein Array Analysis - methods</subject><subject>Protein Array Analysis - standards</subject><subject>Protein Array Analysis - statistics & numerical data</subject><subject>Protein microarray</subject><subject>Rhinitis, Allergic, Seasonal - diagnosis</subject><subject>Rhinitis, Allergic, Seasonal - immunology</subject><subject>Sensitivity and Specificity</subject><subject>Techniques</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUGLFDEQhYMo7rj6A7xILnqyx6qkO53gaVlXXVjwoicPIZOurBm6O2PSM7D_3gwzsDcXiioovlcU7zH2FmGNgOrTdr2N01oAdGvAWuYZW6HuRdMb6J6zFYAQDfaduWCvStkCAIKCl-wCO21k3a_Y7y90oDHtJpoXngJ3vNBc4hIP9JH7NKacJlpy9HyKPieXs3vgrpTaQ8rcjSPle5qbTGWXqvBA_M9-cjO_vb95zV4ENxZ6c56X7NfXm5_X35u7H99ur6_uGt-BWRpErUMbgm6VJ72R0nTKaKEciMG3GKQPqgd0aEgP_SCc2bQS2s2AnXMoSF6yD6e7u5z-7qksdorF0zi6mdK-WNUbKQXIJ0EBfa-VFE-C2CshlTAVxBNYvSklU7C7HCeXHyyCPWZkt7ZmZI8ZWcBaR8278_H9ZqLhUXEOpQLvz4Ar3o0hu9nH8sgprVuhVOU-nziq5h4iZVt8pNnTEDP5xQ4p_ueNf6EXrto</recordid><startdate>20050501</startdate><enddate>20050501</enddate><creator>Lebrun, Stewart J.</creator><creator>Petchpud, Wasinee N.</creator><creator>Hui, Agnas</creator><creator>McLaughlin, Calvin S.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20050501</creationdate><title>Development of a sensitive, colorometric microarray assay for allergen-responsive human IgE</title><author>Lebrun, Stewart J. ; Petchpud, Wasinee N. ; Hui, Agnas ; McLaughlin, Calvin S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c509t-1188f4ff846ce8b339569826a02dc41f3cf6701a19e8d7d2a9b4304bd15aa12e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Allergens - administration & dosage</topic><topic>Allergy testing</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Case-Control Studies</topic><topic>Colorimetry</topic><topic>Dust - immunology</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Enzyme-Linked Immunosorbent Assay - statistics & numerical data</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Fungi - immunology</topic><topic>Humans</topic><topic>Hypersensitivity, Immediate - diagnosis</topic><topic>Hypersensitivity, Immediate - immunology</topic><topic>IgE in vitro test</topic><topic>Immunoglobulin E - analysis</topic><topic>Immunoglobulin E - blood</topic><topic>In Vitro Techniques</topic><topic>Mites - immunology</topic><topic>Molecular immunology</topic><topic>Phleum - immunology</topic><topic>Protein Array Analysis - methods</topic><topic>Protein Array Analysis - standards</topic><topic>Protein Array Analysis - statistics & numerical data</topic><topic>Protein microarray</topic><topic>Rhinitis, Allergic, Seasonal - diagnosis</topic><topic>Rhinitis, Allergic, Seasonal - immunology</topic><topic>Sensitivity and Specificity</topic><topic>Techniques</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lebrun, Stewart J.</creatorcontrib><creatorcontrib>Petchpud, Wasinee N.</creatorcontrib><creatorcontrib>Hui, Agnas</creatorcontrib><creatorcontrib>McLaughlin, Calvin S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lebrun, Stewart J.</au><au>Petchpud, Wasinee N.</au><au>Hui, Agnas</au><au>McLaughlin, Calvin S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a sensitive, colorometric microarray assay for allergen-responsive human IgE</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>2005-05-01</date><risdate>2005</risdate><volume>300</volume><issue>1</issue><spage>24</spage><epage>31</epage><pages>24-31</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><coden>JIMMBG</coden><abstract>With the existence of several thousand unique human allergens, a multiplex format, such as protein microarrays, is an attractive option for allergy screening. To determine the feasibility and sensitivity of using an enzyme-based, colorimetric protein microarray assay, three common allergens (mold, dustmite, grass) were arrayed and added sera assayed for responsive human IgE. Normal, low positive, and negative control samples were assayed to determine optimal reaction parameters. Sensitivity of the assay (in international units, IU) was determined by constructing a standard curve using World Health Organization (WHO) standards. The system described here can reliably detect allergen-specific IgE below 0.35 IU, the current WHO standard cutoff. By taking advantage of the sensitivity of enzyme-linked immunosorbent assays (ELISAs) and the multiplex format of microarrays, we have achieved a high throughput system, capable of screening patients for allergen-susceptibility with optimal sensitivity.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>15893759</pmid><doi>10.1016/j.jim.2005.01.019</doi><tpages>8</tpages></addata></record> |
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| subjects | Allergens - administration & dosage Allergy testing Animals Biological and medical sciences Case-Control Studies Colorimetry Dust - immunology Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - statistics & numerical data Fundamental and applied biological sciences. Psychology Fundamental immunology Fungi - immunology Humans Hypersensitivity, Immediate - diagnosis Hypersensitivity, Immediate - immunology IgE in vitro test Immunoglobulin E - analysis Immunoglobulin E - blood In Vitro Techniques Mites - immunology Molecular immunology Phleum - immunology Protein Array Analysis - methods Protein Array Analysis - standards Protein Array Analysis - statistics & numerical data Protein microarray Rhinitis, Allergic, Seasonal - diagnosis Rhinitis, Allergic, Seasonal - immunology Sensitivity and Specificity Techniques |
| title | Development of a sensitive, colorometric microarray assay for allergen-responsive human IgE |
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