Determination of unconjugated neurosteroids in rat brain regions by liquid chromatography-negative atmospheric pressure ionization mass spectrometry

To simultaneously determine three unconjugated neurosteroids, dehydroepiandrosterone (DHEA) , pregnenolone (PREG), allopregnenolone (AP), from several brain regions of the rat. Neurosteroids were isolated separately in a two steps procedure by using ethyl acetate-n-hexane (90:10) as the first step t...

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Veröffentlicht in:	Yao hsüeh hsüeh pao 2005-03, Vol.40 (3), p.262-266
Hauptverfasser:	Ren, Jin-min, Hou, Yan-ning
Format:	Artikel
Sprache:	chi
Schlagworte:	Amygdala - chemistry Animals Brain Chemistry Chromatography, High Pressure Liquid Corpus Striatum - chemistry Dehydroepiandrosterone - analysis Hippocampus - chemistry Hypothalamus - chemistry Male Mass Spectrometry - methods Nucleus Accumbens - chemistry Prefrontal Cortex - chemistry Pregnenolone - analogs & derivatives Pregnenolone - analysis Rats Rats, Sprague-Dawley Sensitivity and Specificity
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