Effect of buffalo seminal plasma heparin binding protein (HBP) on freezability and in vitro fertility of buffalo cauda spermatozoa
The study was conducted to assess the effect of heparin binding seminal plasma proteins (HBP) on freezability and in vitro fertilizing ability of buffalo cauda epididymal spermatozoa. Spermatozoal motility, viability and acrosomal integrity at prefreeze and post-thaw stages were studied. The in vitr...
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| Veröffentlicht in: | Animal reproduction science 2006-06, Vol.93 (1), p.124-133 |
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| creator | Harshan, Hiron M. Singh, L.P. Arangasamy, A. Ansari, M.R. Kumar, Satish |
| description | The study was conducted to assess the effect of heparin binding seminal plasma proteins (HBP) on freezability and in vitro fertilizing ability of buffalo cauda epididymal spermatozoa. Spermatozoal motility, viability and acrosomal integrity at prefreeze and post-thaw stages were studied. The in vitro fertilizing ability of spermatozoa was assessed by the application of two tests, i.e. bovine cervical mucus penetration test (BCMPT) and hypo-osmotic sperm swelling test (HOST). HBP isolated from buffalo seminal plasma and maintained in the laboratory were used for the study. Twelve pairs of epididymis from adult buffaloes slaughtered at the local abattoir were used for the study. The results indicated that HBP addition improved the progressive motility, BCMPT and HOST response at prefreeze level. HBP at a concentration of 40
μg/ml showed better results than HBP at a concentration of 80
μg/ml. However, subjecting the HBP treated spermatozoa to cryopreservation resulted in significant reduction of motility, viability, acrosomal integrity and response to BCMPT and HOST in the HBP treated groups when compared to those in control group. The deleterious effect of HBP was found to be concentration dependent with the higher concentration causing higher post-thaw damage. |
| doi_str_mv | 10.1016/j.anireprosci.2005.07.010 |
| format | Article |
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μg/ml showed better results than HBP at a concentration of 80
μg/ml. However, subjecting the HBP treated spermatozoa to cryopreservation resulted in significant reduction of motility, viability, acrosomal integrity and response to BCMPT and HOST in the HBP treated groups when compared to those in control group. The deleterious effect of HBP was found to be concentration dependent with the higher concentration causing higher post-thaw damage.</description><identifier>ISSN: 0378-4320</identifier><identifier>EISSN: 1873-2232</identifier><identifier>DOI: 10.1016/j.anireprosci.2005.07.010</identifier><identifier>PMID: 16143473</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>acrosome ; Acrosome - physiology ; Animals ; binding proteins ; Buffalo ; buffaloes ; Buffaloes - physiology ; cauda epididymidis ; Cauda spermatozoa ; cryopreservation ; Cryopreservation - methods ; Cryopreservation - veterinary ; dosage ; Female ; Fertility ; Fertility - physiology ; Freezability ; freezing quality ; heparin ; Heparin - metabolism ; Heparin binding proteins ; in vitro fertilization ; Male ; male fertility ; males ; Membrane Proteins - metabolism ; Membrane Proteins - pharmacology ; osmotic pressure ; semen ; Semen Preservation - methods ; Semen Preservation - veterinary ; Seminal plasma ; Seminal Plasma Proteins - metabolism ; Seminal Plasma Proteins - pharmacology ; Sperm Count - veterinary ; sperm motility ; Sperm Motility - physiology ; spermatozoa ; Spermatozoa - physiology ; thawing ; viability</subject><ispartof>Animal reproduction science, 2006-06, Vol.93 (1), p.124-133</ispartof><rights>2005 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c430t-4199d6be815b36af032acb4499e2844a35ab11e0a02c17915cb5007a272dc23f3</citedby><cites>FETCH-LOGICAL-c430t-4199d6be815b36af032acb4499e2844a35ab11e0a02c17915cb5007a272dc23f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378432005002071$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16143473$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Harshan, Hiron M.</creatorcontrib><creatorcontrib>Singh, L.P.</creatorcontrib><creatorcontrib>Arangasamy, A.</creatorcontrib><creatorcontrib>Ansari, M.R.</creatorcontrib><creatorcontrib>Kumar, Satish</creatorcontrib><title>Effect of buffalo seminal plasma heparin binding protein (HBP) on freezability and in vitro fertility of buffalo cauda spermatozoa</title><title>Animal reproduction science</title><addtitle>Anim Reprod Sci</addtitle><description>The study was conducted to assess the effect of heparin binding seminal plasma proteins (HBP) on freezability and in vitro fertilizing ability of buffalo cauda epididymal spermatozoa. Spermatozoal motility, viability and acrosomal integrity at prefreeze and post-thaw stages were studied. The in vitro fertilizing ability of spermatozoa was assessed by the application of two tests, i.e. bovine cervical mucus penetration test (BCMPT) and hypo-osmotic sperm swelling test (HOST). HBP isolated from buffalo seminal plasma and maintained in the laboratory were used for the study. Twelve pairs of epididymis from adult buffaloes slaughtered at the local abattoir were used for the study. The results indicated that HBP addition improved the progressive motility, BCMPT and HOST response at prefreeze level. HBP at a concentration of 40
μg/ml showed better results than HBP at a concentration of 80
μg/ml. However, subjecting the HBP treated spermatozoa to cryopreservation resulted in significant reduction of motility, viability, acrosomal integrity and response to BCMPT and HOST in the HBP treated groups when compared to those in control group. The deleterious effect of HBP was found to be concentration dependent with the higher concentration causing higher post-thaw damage.</description><subject>acrosome</subject><subject>Acrosome - physiology</subject><subject>Animals</subject><subject>binding proteins</subject><subject>Buffalo</subject><subject>buffaloes</subject><subject>Buffaloes - physiology</subject><subject>cauda epididymidis</subject><subject>Cauda spermatozoa</subject><subject>cryopreservation</subject><subject>Cryopreservation - methods</subject><subject>Cryopreservation - veterinary</subject><subject>dosage</subject><subject>Female</subject><subject>Fertility</subject><subject>Fertility - physiology</subject><subject>Freezability</subject><subject>freezing quality</subject><subject>heparin</subject><subject>Heparin - metabolism</subject><subject>Heparin binding proteins</subject><subject>in vitro fertilization</subject><subject>Male</subject><subject>male fertility</subject><subject>males</subject><subject>Membrane Proteins - metabolism</subject><subject>Membrane Proteins - pharmacology</subject><subject>osmotic pressure</subject><subject>semen</subject><subject>Semen Preservation - methods</subject><subject>Semen Preservation - veterinary</subject><subject>Seminal plasma</subject><subject>Seminal Plasma Proteins - metabolism</subject><subject>Seminal Plasma Proteins - pharmacology</subject><subject>Sperm Count - veterinary</subject><subject>sperm motility</subject><subject>Sperm Motility - physiology</subject><subject>spermatozoa</subject><subject>Spermatozoa - physiology</subject><subject>thawing</subject><subject>viability</subject><issn>0378-4320</issn><issn>1873-2232</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhiMEotvCXwBzQXBIGH8kWR_LqqVIlUCCnq2JMy5e5Qs7qdQe-eV4yUr0BifLnmf8evxk2RsOBQdefdgXOPhAUxij9YUAKAuoC-DwJNvwbS1zIaR4mm1A1ttcSQEn2WmMewCoq0o_z054xZVUtdxkvy6cIzuz0bFmcQ67kUXq_YAdmzqMPbIfNGHwA2v80PrhlqXUmdL-3dXHr-_ZODAXiB6w8Z2f7xkOLUvFOz-HkTkK83r86HqLS4ssThR6nMeHEV9kz1Ih0svjepbdXF58313l118-fd6dX-dWSZhzxbVuq4a2vGxkhQ6kQNsopTWJrVIoS2w4J0AQlteal7Yp08AoatFaIZ08y96u96YJfi4UZ9P7aKnrcKBxiaaqtSjrrfwnyLVSoHSZQL2CNpmIgZyZgu8x3BsO5mDK7M0jU-ZgykBtkqnU--oYsjQ9tX87j2oS8HoFHI4Gb4OP5uabAC4PFqH6Q-xWgtKv3XkKJoXQYKlNkXY27ej_4yG_AbNrtTo</recordid><startdate>20060601</startdate><enddate>20060601</enddate><creator>Harshan, Hiron M.</creator><creator>Singh, L.P.</creator><creator>Arangasamy, A.</creator><creator>Ansari, M.R.</creator><creator>Kumar, Satish</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20060601</creationdate><title>Effect of buffalo seminal plasma heparin binding protein (HBP) on freezability and in vitro fertility of buffalo cauda spermatozoa</title><author>Harshan, Hiron M. ; Singh, L.P. ; Arangasamy, A. ; Ansari, M.R. ; Kumar, Satish</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c430t-4199d6be815b36af032acb4499e2844a35ab11e0a02c17915cb5007a272dc23f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>acrosome</topic><topic>Acrosome - physiology</topic><topic>Animals</topic><topic>binding proteins</topic><topic>Buffalo</topic><topic>buffaloes</topic><topic>Buffaloes - physiology</topic><topic>cauda epididymidis</topic><topic>Cauda spermatozoa</topic><topic>cryopreservation</topic><topic>Cryopreservation - methods</topic><topic>Cryopreservation - veterinary</topic><topic>dosage</topic><topic>Female</topic><topic>Fertility</topic><topic>Fertility - physiology</topic><topic>Freezability</topic><topic>freezing quality</topic><topic>heparin</topic><topic>Heparin - metabolism</topic><topic>Heparin binding proteins</topic><topic>in vitro fertilization</topic><topic>Male</topic><topic>male fertility</topic><topic>males</topic><topic>Membrane Proteins - metabolism</topic><topic>Membrane Proteins - pharmacology</topic><topic>osmotic pressure</topic><topic>semen</topic><topic>Semen Preservation - methods</topic><topic>Semen Preservation - veterinary</topic><topic>Seminal plasma</topic><topic>Seminal Plasma Proteins - metabolism</topic><topic>Seminal Plasma Proteins - pharmacology</topic><topic>Sperm Count - veterinary</topic><topic>sperm motility</topic><topic>Sperm Motility - physiology</topic><topic>spermatozoa</topic><topic>Spermatozoa - physiology</topic><topic>thawing</topic><topic>viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Harshan, Hiron M.</creatorcontrib><creatorcontrib>Singh, L.P.</creatorcontrib><creatorcontrib>Arangasamy, A.</creatorcontrib><creatorcontrib>Ansari, M.R.</creatorcontrib><creatorcontrib>Kumar, Satish</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Animal reproduction science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Harshan, Hiron M.</au><au>Singh, L.P.</au><au>Arangasamy, A.</au><au>Ansari, M.R.</au><au>Kumar, Satish</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of buffalo seminal plasma heparin binding protein (HBP) on freezability and in vitro fertility of buffalo cauda spermatozoa</atitle><jtitle>Animal reproduction science</jtitle><addtitle>Anim Reprod Sci</addtitle><date>2006-06-01</date><risdate>2006</risdate><volume>93</volume><issue>1</issue><spage>124</spage><epage>133</epage><pages>124-133</pages><issn>0378-4320</issn><eissn>1873-2232</eissn><abstract>The study was conducted to assess the effect of heparin binding seminal plasma proteins (HBP) on freezability and in vitro fertilizing ability of buffalo cauda epididymal spermatozoa. Spermatozoal motility, viability and acrosomal integrity at prefreeze and post-thaw stages were studied. The in vitro fertilizing ability of spermatozoa was assessed by the application of two tests, i.e. bovine cervical mucus penetration test (BCMPT) and hypo-osmotic sperm swelling test (HOST). HBP isolated from buffalo seminal plasma and maintained in the laboratory were used for the study. Twelve pairs of epididymis from adult buffaloes slaughtered at the local abattoir were used for the study. The results indicated that HBP addition improved the progressive motility, BCMPT and HOST response at prefreeze level. HBP at a concentration of 40
μg/ml showed better results than HBP at a concentration of 80
μg/ml. However, subjecting the HBP treated spermatozoa to cryopreservation resulted in significant reduction of motility, viability, acrosomal integrity and response to BCMPT and HOST in the HBP treated groups when compared to those in control group. The deleterious effect of HBP was found to be concentration dependent with the higher concentration causing higher post-thaw damage.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>16143473</pmid><doi>10.1016/j.anireprosci.2005.07.010</doi><tpages>10</tpages></addata></record> |
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| ispartof | Animal reproduction science, 2006-06, Vol.93 (1), p.124-133 |
| issn | 0378-4320 1873-2232 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | acrosome Acrosome - physiology Animals binding proteins Buffalo buffaloes Buffaloes - physiology cauda epididymidis Cauda spermatozoa cryopreservation Cryopreservation - methods Cryopreservation - veterinary dosage Female Fertility Fertility - physiology Freezability freezing quality heparin Heparin - metabolism Heparin binding proteins in vitro fertilization Male male fertility males Membrane Proteins - metabolism Membrane Proteins - pharmacology osmotic pressure semen Semen Preservation - methods Semen Preservation - veterinary Seminal plasma Seminal Plasma Proteins - metabolism Seminal Plasma Proteins - pharmacology Sperm Count - veterinary sperm motility Sperm Motility - physiology spermatozoa Spermatozoa - physiology thawing viability |
| title | Effect of buffalo seminal plasma heparin binding protein (HBP) on freezability and in vitro fertility of buffalo cauda spermatozoa |
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